Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification

Autores
Torino, Maria Ines; Sesma, Fernando Juan Manuel; Font, Graciela Maria
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Growth and exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 was evaluated in semi-defined media. Lactose (as carbon source) and CaCl2 proved to be the major stimulating factors for an optimal EPS synthesis while the highest specific EPS yield was obtained when CaCl2 and MnSO4 were the only salts added to the medium. Both EPS and cell growth were slightly affected by the nitrogen source. The analysis of the interference components (EPS-eq) in uninoculated media revealed an overestimation in EPS of ca. 76% due to beef extract and yeast extract, and of 64% due to the presence of peptone. Nevertheless, in fermented cultures, the level of interference may be lower since nitrogen compounds are used by the microorganism during growth and residual lactose showed to be easily removed by dialysis before EPS quantification.
Fil: Torino, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
LACTOBACILLUS HELVETICUS
EXOPOLYSACCHARIDE PRODUCTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51607
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantificationTorino, Maria InesSesma, Fernando Juan ManuelFont, Graciela MariaLACTOBACILLUS HELVETICUSEXOPOLYSACCHARIDE PRODUCTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Growth and exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 was evaluated in semi-defined media. Lactose (as carbon source) and CaCl2 proved to be the major stimulating factors for an optimal EPS synthesis while the highest specific EPS yield was obtained when CaCl2 and MnSO4 were the only salts added to the medium. Both EPS and cell growth were slightly affected by the nitrogen source. The analysis of the interference components (EPS-eq) in uninoculated media revealed an overestimation in EPS of ca. 76% due to beef extract and yeast extract, and of 64% due to the presence of peptone. Nevertheless, in fermented cultures, the level of interference may be lower since nitrogen compounds are used by the microorganism during growth and residual lactose showed to be easily removed by dialysis before EPS quantification.Fil: Torino, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaAVA-Verlag2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51607Torino, Maria Ines; Sesma, Fernando Juan Manuel; Font, Graciela Maria; Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification; AVA-Verlag; Milchwissenschaft-milk Science International; 55; 6; 12-2000; 314-3160026-37882567-9538CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.milkscience.de/menu-top/about-the-journal/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:34Zoai:ri.conicet.gov.ar:11336/51607instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:34.947CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
title Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
spellingShingle Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
Torino, Maria Ines
LACTOBACILLUS HELVETICUS
EXOPOLYSACCHARIDE PRODUCTION
title_short Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
title_full Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
title_fullStr Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
title_full_unstemmed Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
title_sort Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification
dc.creator.none.fl_str_mv Torino, Maria Ines
Sesma, Fernando Juan Manuel
Font, Graciela Maria
author Torino, Maria Ines
author_facet Torino, Maria Ines
Sesma, Fernando Juan Manuel
Font, Graciela Maria
author_role author
author2 Sesma, Fernando Juan Manuel
Font, Graciela Maria
author2_role author
author
dc.subject.none.fl_str_mv LACTOBACILLUS HELVETICUS
EXOPOLYSACCHARIDE PRODUCTION
topic LACTOBACILLUS HELVETICUS
EXOPOLYSACCHARIDE PRODUCTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Growth and exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 was evaluated in semi-defined media. Lactose (as carbon source) and CaCl2 proved to be the major stimulating factors for an optimal EPS synthesis while the highest specific EPS yield was obtained when CaCl2 and MnSO4 were the only salts added to the medium. Both EPS and cell growth were slightly affected by the nitrogen source. The analysis of the interference components (EPS-eq) in uninoculated media revealed an overestimation in EPS of ca. 76% due to beef extract and yeast extract, and of 64% due to the presence of peptone. Nevertheless, in fermented cultures, the level of interference may be lower since nitrogen compounds are used by the microorganism during growth and residual lactose showed to be easily removed by dialysis before EPS quantification.
Fil: Torino, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Growth and exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 was evaluated in semi-defined media. Lactose (as carbon source) and CaCl2 proved to be the major stimulating factors for an optimal EPS synthesis while the highest specific EPS yield was obtained when CaCl2 and MnSO4 were the only salts added to the medium. Both EPS and cell growth were slightly affected by the nitrogen source. The analysis of the interference components (EPS-eq) in uninoculated media revealed an overestimation in EPS of ca. 76% due to beef extract and yeast extract, and of 64% due to the presence of peptone. Nevertheless, in fermented cultures, the level of interference may be lower since nitrogen compounds are used by the microorganism during growth and residual lactose showed to be easily removed by dialysis before EPS quantification.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51607
Torino, Maria Ines; Sesma, Fernando Juan Manuel; Font, Graciela Maria; Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification; AVA-Verlag; Milchwissenschaft-milk Science International; 55; 6; 12-2000; 314-316
0026-3788
2567-9538
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51607
identifier_str_mv Torino, Maria Ines; Sesma, Fernando Juan Manuel; Font, Graciela Maria; Semi-defined media for the exopolysaccharide (EPS) production by Lactobacillus helveticus ATCC 15807 and evaluation of the components interfering with the EPS quantification; AVA-Verlag; Milchwissenschaft-milk Science International; 55; 6; 12-2000; 314-316
0026-3788
2567-9538
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.milkscience.de/menu-top/about-the-journal/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv AVA-Verlag
publisher.none.fl_str_mv AVA-Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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