Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice

Autores
Lackey, Atreju I.; Chen, Tina; Zhou, Yin X.; Bottasso Arias, Natalia María; Doran, Justine M.; Zacharisen, Sophia M.; Gajda, Angela M.; Jonsson, William O.; Córsico, Betina; Anthony, Tracy G.; Joseph, Laurie B.; Storch, Judith
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intestinal- fatty acid binding protein (IFABP; FABP2) is a 15-kDa intracellular protein abundantly present in the cytosol of the small intestinal (SI) enterocyte. High-fat (HF) feeding of IFABP-/- mice resulted in reduced weight gain and fat mass relative to wild-type (WT) mice. Here, we examined intestinal properties that may underlie the observed lean phenotype of high fat-fed IFABP-/- mice. No alterations in fecal lipid content were found, suggesting that the IFABP-/- mice are not malabsorbing dietary fat. However, the total excreted fecal mass, normalized to food intake, was increased for the IFABP-/- mice relative to WT mice. Moreover, intestinal transit time was more rapid in the IFABP-/- mice. IFABP-/- mice displayed a shortened average villus length, a thinner muscularis layer, reduced goblet cell density, and reduced Paneth cell abundance. The number of proliferating cells in the crypts of IFABP-/- mice did not differ from that of WT mice, suggesting that the blunt villi phenotype is not due to alterations in proliferation. IFABP-/- mice were observed to have altered expression of genes and proteins related to intestinal structure, while immunohistochemical analyses revealed increased staining for markers of inflammation. Taken together, these studies indicate that the ablation of IFABP, coupled with high-fat feeding, leads to changes in gut motility and morphology, which likely contribute to the relatively leaner phenotype occurring at the whole-body level. Thus, IFABP is likely involved in dietary lipid sensing and signaling, influencing intestinal motility, intestinal structure, and nutrient absorption, thereby impacting systemic energy metabolism.
Fil: Lackey, Atreju I.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Chen, Tina. Rutgers University; Estados Unidos
Fil: Zhou, Yin X.. Rutgers University; Estados Unidos
Fil: Bottasso Arias, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Doran, Justine M.. Rutgers University; Estados Unidos
Fil: Zacharisen, Sophia M.. Rutgers University; Estados Unidos
Fil: Gajda, Angela M.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Jonsson, William O.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Córsico, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Anthony, Tracy G.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Joseph, Laurie B.. Rutgers Center for Lipid Research; Estados Unidos. Rutgers University; Estados Unidos
Fil: Storch, Judith. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Materia
IFABP
INTESTINE
LIPID
MORPHOLOGY
NUTRITION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143771
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/143771
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null miceLackey, Atreju I.Chen, TinaZhou, Yin X.Bottasso Arias, Natalia MaríaDoran, Justine M.Zacharisen, Sophia M.Gajda, Angela M.Jonsson, William O.Córsico, BetinaAnthony, Tracy G.Joseph, Laurie B.Storch, JudithIFABPINTESTINELIPIDMORPHOLOGYNUTRITIONhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Intestinal- fatty acid binding protein (IFABP; FABP2) is a 15-kDa intracellular protein abundantly present in the cytosol of the small intestinal (SI) enterocyte. High-fat (HF) feeding of IFABP-/- mice resulted in reduced weight gain and fat mass relative to wild-type (WT) mice. Here, we examined intestinal properties that may underlie the observed lean phenotype of high fat-fed IFABP-/- mice. No alterations in fecal lipid content were found, suggesting that the IFABP-/- mice are not malabsorbing dietary fat. However, the total excreted fecal mass, normalized to food intake, was increased for the IFABP-/- mice relative to WT mice. Moreover, intestinal transit time was more rapid in the IFABP-/- mice. IFABP-/- mice displayed a shortened average villus length, a thinner muscularis layer, reduced goblet cell density, and reduced Paneth cell abundance. The number of proliferating cells in the crypts of IFABP-/- mice did not differ from that of WT mice, suggesting that the blunt villi phenotype is not due to alterations in proliferation. IFABP-/- mice were observed to have altered expression of genes and proteins related to intestinal structure, while immunohistochemical analyses revealed increased staining for markers of inflammation. Taken together, these studies indicate that the ablation of IFABP, coupled with high-fat feeding, leads to changes in gut motility and morphology, which likely contribute to the relatively leaner phenotype occurring at the whole-body level. Thus, IFABP is likely involved in dietary lipid sensing and signaling, influencing intestinal motility, intestinal structure, and nutrient absorption, thereby impacting systemic energy metabolism.Fil: Lackey, Atreju I.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados UnidosFil: Chen, Tina. Rutgers University; Estados UnidosFil: Zhou, Yin X.. Rutgers University; Estados UnidosFil: Bottasso Arias, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Doran, Justine M.. Rutgers University; Estados UnidosFil: Zacharisen, Sophia M.. Rutgers University; Estados UnidosFil: Gajda, Angela M.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados UnidosFil: Jonsson, William O.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados UnidosFil: Córsico, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Anthony, Tracy G.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados UnidosFil: Joseph, Laurie B.. Rutgers Center for Lipid Research; Estados Unidos. Rutgers University; Estados UnidosFil: Storch, Judith. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados UnidosAmerican Physiological Society2020-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143771Lackey, Atreju I.; Chen, Tina; Zhou, Yin X.; Bottasso Arias, Natalia María; Doran, Justine M.; et al.; Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 318; 3; 27-2-2020; G518-G5300193-18571522-1547CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1152/ajpgi.00120.2019info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpgi.00120.2019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:01Zoai:ri.conicet.gov.ar:11336/143771instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:01.48CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
spellingShingle Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
Lackey, Atreju I.
IFABP
INTESTINE
LIPID
MORPHOLOGY
NUTRITION
title_short Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_full Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_fullStr Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_full_unstemmed Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
title_sort Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice
dc.creator.none.fl_str_mv Lackey, Atreju I.
Chen, Tina
Zhou, Yin X.
Bottasso Arias, Natalia María
Doran, Justine M.
Zacharisen, Sophia M.
Gajda, Angela M.
Jonsson, William O.
Córsico, Betina
Anthony, Tracy G.
Joseph, Laurie B.
Storch, Judith
author Lackey, Atreju I.
author_facet Lackey, Atreju I.
Chen, Tina
Zhou, Yin X.
Bottasso Arias, Natalia María
Doran, Justine M.
Zacharisen, Sophia M.
Gajda, Angela M.
Jonsson, William O.
Córsico, Betina
Anthony, Tracy G.
Joseph, Laurie B.
Storch, Judith
author_role author
author2 Chen, Tina
Zhou, Yin X.
Bottasso Arias, Natalia María
Doran, Justine M.
Zacharisen, Sophia M.
Gajda, Angela M.
Jonsson, William O.
Córsico, Betina
Anthony, Tracy G.
Joseph, Laurie B.
Storch, Judith
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IFABP
INTESTINE
LIPID
MORPHOLOGY
NUTRITION
topic IFABP
INTESTINE
LIPID
MORPHOLOGY
NUTRITION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Intestinal- fatty acid binding protein (IFABP; FABP2) is a 15-kDa intracellular protein abundantly present in the cytosol of the small intestinal (SI) enterocyte. High-fat (HF) feeding of IFABP-/- mice resulted in reduced weight gain and fat mass relative to wild-type (WT) mice. Here, we examined intestinal properties that may underlie the observed lean phenotype of high fat-fed IFABP-/- mice. No alterations in fecal lipid content were found, suggesting that the IFABP-/- mice are not malabsorbing dietary fat. However, the total excreted fecal mass, normalized to food intake, was increased for the IFABP-/- mice relative to WT mice. Moreover, intestinal transit time was more rapid in the IFABP-/- mice. IFABP-/- mice displayed a shortened average villus length, a thinner muscularis layer, reduced goblet cell density, and reduced Paneth cell abundance. The number of proliferating cells in the crypts of IFABP-/- mice did not differ from that of WT mice, suggesting that the blunt villi phenotype is not due to alterations in proliferation. IFABP-/- mice were observed to have altered expression of genes and proteins related to intestinal structure, while immunohistochemical analyses revealed increased staining for markers of inflammation. Taken together, these studies indicate that the ablation of IFABP, coupled with high-fat feeding, leads to changes in gut motility and morphology, which likely contribute to the relatively leaner phenotype occurring at the whole-body level. Thus, IFABP is likely involved in dietary lipid sensing and signaling, influencing intestinal motility, intestinal structure, and nutrient absorption, thereby impacting systemic energy metabolism.
Fil: Lackey, Atreju I.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Chen, Tina. Rutgers University; Estados Unidos
Fil: Zhou, Yin X.. Rutgers University; Estados Unidos
Fil: Bottasso Arias, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Doran, Justine M.. Rutgers University; Estados Unidos
Fil: Zacharisen, Sophia M.. Rutgers University; Estados Unidos
Fil: Gajda, Angela M.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Jonsson, William O.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Córsico, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Anthony, Tracy G.. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
Fil: Joseph, Laurie B.. Rutgers Center for Lipid Research; Estados Unidos. Rutgers University; Estados Unidos
Fil: Storch, Judith. Rutgers University; Estados Unidos. Rutgers Center for Lipid Research; Estados Unidos
description Intestinal- fatty acid binding protein (IFABP; FABP2) is a 15-kDa intracellular protein abundantly present in the cytosol of the small intestinal (SI) enterocyte. High-fat (HF) feeding of IFABP-/- mice resulted in reduced weight gain and fat mass relative to wild-type (WT) mice. Here, we examined intestinal properties that may underlie the observed lean phenotype of high fat-fed IFABP-/- mice. No alterations in fecal lipid content were found, suggesting that the IFABP-/- mice are not malabsorbing dietary fat. However, the total excreted fecal mass, normalized to food intake, was increased for the IFABP-/- mice relative to WT mice. Moreover, intestinal transit time was more rapid in the IFABP-/- mice. IFABP-/- mice displayed a shortened average villus length, a thinner muscularis layer, reduced goblet cell density, and reduced Paneth cell abundance. The number of proliferating cells in the crypts of IFABP-/- mice did not differ from that of WT mice, suggesting that the blunt villi phenotype is not due to alterations in proliferation. IFABP-/- mice were observed to have altered expression of genes and proteins related to intestinal structure, while immunohistochemical analyses revealed increased staining for markers of inflammation. Taken together, these studies indicate that the ablation of IFABP, coupled with high-fat feeding, leads to changes in gut motility and morphology, which likely contribute to the relatively leaner phenotype occurring at the whole-body level. Thus, IFABP is likely involved in dietary lipid sensing and signaling, influencing intestinal motility, intestinal structure, and nutrient absorption, thereby impacting systemic energy metabolism.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143771
Lackey, Atreju I.; Chen, Tina; Zhou, Yin X.; Bottasso Arias, Natalia María; Doran, Justine M.; et al.; Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 318; 3; 27-2-2020; G518-G530
0193-1857
1522-1547
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143771
identifier_str_mv Lackey, Atreju I.; Chen, Tina; Zhou, Yin X.; Bottasso Arias, Natalia María; Doran, Justine M.; et al.; Mechanisms underlying reduced weight gain in intestinal fatty acid-binding protein (IFABP) null mice; American Physiological Society; American Journal of Physiology-gastrointestinal and Liver Physiology; 318; 3; 27-2-2020; G518-G530
0193-1857
1522-1547
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpgi.00120.2019
info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpgi.00120.2019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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