Proteomic atlas of organ vasculopathies triggered by Staphylococcus aureus sepsis

• Autores: Gómez Toledo, Alejandro; Golden, Gregory; Rosa Campos, Alexandre; Cuello, Héctor Adrián; Sorrentino, James; Lewis, Nathan; Varki, Nissi; Nizet, Victor; Smith, Jeffrey W.; Esko, Jeffrey D.
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Proteomics profiling; Sepsis; Vasculopathies; Staphylococcus
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Sepsis is a life-threatening condition triggered by a dysregulated host response to microbial infection resulting in vascular dysfunction, organ failure and death. Here we provide a semi-quantitative atlas of the murine vascular cell-surface proteome at the organ level, and how it changes during sepsis. Using in vivo chemical labeling and high-resolution mass spectrometry, we demonstrate the presence of a vascular proteome that is perfusable and shared across multiple organs. This proteome is enriched in membrane-anchored proteins, including multiple regulators of endothelial barrier functions and innate immunity. Further, we automated our workflows and applied them to a murine model of methicillin-resistant Staphylococcus aureus (MRSA) sepsis to unravel changes during systemic inflammatory responses. We provide an organ-specific atlas of both systemic and local changes of the vascular proteome triggered by sepsis. Collectively, the data indicates that MRSA-sepsis triggers extensive proteome remodeling of the vascular cell surfaces, in a tissue-specific manner.
Fil: Gómez Toledo, Alejandro. University of California; Estados Unidos
Fil: Golden, Gregory. University of California; Estados Unidos
Fil: Rosa Campos, Alexandre. Sanford-Burnham-Prebys Medical Discovery Institute; Estados Unidos
Fil: Cuello, Héctor Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Sorrentino, James. University of California at San Diego; Estados Unidos
Fil: Lewis, Nathan. University of California at San Diego; Estados Unidos
Fil: Varki, Nissi. University of California at San Diego; Estados Unidos
Fil: Nizet, Victor. University of California at San Diego; Estados Unidos
Fil: Smith, Jeffrey W.. Sanford-Burnham-Prebys Medical Discovery Institute; Estados Unidos
Fil: Esko, Jeffrey D.. University of California; Estados Unidos