Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish

Autores
Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Zolessi, F.R.; Casco, Victor Hugo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminantsin freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD)and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show thepresence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, amarker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker,was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxideinducedDNA damage, showing a dose-dependent response. We observed a positive comet assay response to CMthat was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and catmRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induceapoptosis in retinal cells.
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zolessi, F.R.. Universidad de la Republica.; Uruguay
Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos; Argentina
Materia
DNA damage
Apoptosis
Apoptosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102155
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafishParavani, Enrique ValentinSimoniello, Maria FernandaPoletta, Gisela LauraZolessi, F.R.Casco, Victor HugoDNA damageApoptosisApoptosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminantsin freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD)and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show thepresence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, amarker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker,was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxideinducedDNA damage, showing a dose-dependent response. We observed a positive comet assay response to CMthat was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and catmRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induceapoptosis in retinal cells.Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos; ArgentinaFil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; ArgentinaFil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zolessi, F.R.. Universidad de la Republica.; UruguayFil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos; ArgentinaElsevier Science2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102155Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Zolessi, F.R.; Casco, Victor Hugo; Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 826; 2018; 25-321383-5718CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2017.12.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:59:52Zoai:ri.conicet.gov.ar:11336/102155instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:59:52.599CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
title Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
spellingShingle Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
Paravani, Enrique Valentin
DNA damage
Apoptosis
Apoptosis
title_short Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
title_full Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
title_fullStr Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
title_full_unstemmed Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
title_sort Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
dc.creator.none.fl_str_mv Paravani, Enrique Valentin
Simoniello, Maria Fernanda
Poletta, Gisela Laura
Zolessi, F.R.
Casco, Victor Hugo
author Paravani, Enrique Valentin
author_facet Paravani, Enrique Valentin
Simoniello, Maria Fernanda
Poletta, Gisela Laura
Zolessi, F.R.
Casco, Victor Hugo
author_role author
author2 Simoniello, Maria Fernanda
Poletta, Gisela Laura
Zolessi, F.R.
Casco, Victor Hugo
author2_role author
author
author
author
dc.subject.none.fl_str_mv DNA damage
Apoptosis
Apoptosis
topic DNA damage
Apoptosis
Apoptosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminantsin freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD)and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show thepresence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, amarker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker,was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxideinducedDNA damage, showing a dose-dependent response. We observed a positive comet assay response to CMthat was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and catmRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induceapoptosis in retinal cells.
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zolessi, F.R.. Universidad de la Republica.; Uruguay
Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos; Argentina
description Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminantsin freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD)and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show thepresence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, amarker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker,was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxideinducedDNA damage, showing a dose-dependent response. We observed a positive comet assay response to CMthat was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and catmRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induceapoptosis in retinal cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102155
Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Zolessi, F.R.; Casco, Victor Hugo; Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 826; 2018; 25-32
1383-5718
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102155
identifier_str_mv Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Zolessi, F.R.; Casco, Victor Hugo; Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 826; 2018; 25-32
1383-5718
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2017.12.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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