Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells

Autores
Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Casco, Victor Hugo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cypermethrin (CYP) is a synthetic pyrethroid insecticide, used to control pests in domestic, industrial and agricultural environments. According to recent reports, it is one of the most common contaminants in freshwater aquatic systems. The aim of this study was to evaluate its potential genotoxic effect and the activation of the superoxide dismutase (SOD) and catalase (CAT) systems of adult zebrafish gill cells after in vivo exposure. The comet assay (CA) demonstrated that gill cells are sensitive to DNA damage after in vitro exposure to hydrogen peroxide (H 2 O 2 ), showing a dose-dependent response. We also found an increase in DNA damage of gill cells following a dose- and time-dependent treatment with CYP. Moreover, it was verified that SOD and CAT activities significantly increased after exposure to 0.6 µg/L CYP, both during six and nine days. The same treatment caused a significant up-regulation of the mRNA levels of Mn-sod and cat genes. These data indicate that CYP causes gill cell´s DNA damage and oxidative stress, modifying the activities of the enzymes responsible for maintaining ROS balance, as well as in their corresponding gene expression levels.
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral; Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Casco, Victor Hugo. Ibb-conicet-uner; Argentina. Universidad Nacional de Entre Ríos; Argentina
Materia
CYPERMETHRIN
DNA DAMAGE
GENE EXPRESSION
GILL CELLS
OXIDATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111269
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/111269
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cellsParavani, Enrique ValentinSimoniello, Maria FernandaPoletta, Gisela LauraCasco, Victor HugoCYPERMETHRINDNA DAMAGEGENE EXPRESSIONGILL CELLSOXIDATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cypermethrin (CYP) is a synthetic pyrethroid insecticide, used to control pests in domestic, industrial and agricultural environments. According to recent reports, it is one of the most common contaminants in freshwater aquatic systems. The aim of this study was to evaluate its potential genotoxic effect and the activation of the superoxide dismutase (SOD) and catalase (CAT) systems of adult zebrafish gill cells after in vivo exposure. The comet assay (CA) demonstrated that gill cells are sensitive to DNA damage after in vitro exposure to hydrogen peroxide (H 2 O 2 ), showing a dose-dependent response. We also found an increase in DNA damage of gill cells following a dose- and time-dependent treatment with CYP. Moreover, it was verified that SOD and CAT activities significantly increased after exposure to 0.6 µg/L CYP, both during six and nine days. The same treatment caused a significant up-regulation of the mRNA levels of Mn-sod and cat genes. These data indicate that CYP causes gill cell´s DNA damage and oxidative stress, modifying the activities of the enzymes responsible for maintaining ROS balance, as well as in their corresponding gene expression levels.Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos; ArgentinaFil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral; ArgentinaFil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Casco, Victor Hugo. Ibb-conicet-uner; Argentina. Universidad Nacional de Entre Ríos; ArgentinaAcademic Press Inc Elsevier Science2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111269Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Casco, Victor Hugo; Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 173; 5-2019; 1-70147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2019.02.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:43Zoai:ri.conicet.gov.ar:11336/111269instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:30:43.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
title Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
spellingShingle Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
Paravani, Enrique Valentin
CYPERMETHRIN
DNA DAMAGE
GENE EXPRESSION
GILL CELLS
OXIDATIVE STRESS
title_short Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
title_full Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
title_fullStr Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
title_full_unstemmed Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
title_sort Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells
dc.creator.none.fl_str_mv Paravani, Enrique Valentin
Simoniello, Maria Fernanda
Poletta, Gisela Laura
Casco, Victor Hugo
author Paravani, Enrique Valentin
author_facet Paravani, Enrique Valentin
Simoniello, Maria Fernanda
Poletta, Gisela Laura
Casco, Victor Hugo
author_role author
author2 Simoniello, Maria Fernanda
Poletta, Gisela Laura
Casco, Victor Hugo
author2_role author
author
author
dc.subject.none.fl_str_mv CYPERMETHRIN
DNA DAMAGE
GENE EXPRESSION
GILL CELLS
OXIDATIVE STRESS
topic CYPERMETHRIN
DNA DAMAGE
GENE EXPRESSION
GILL CELLS
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cypermethrin (CYP) is a synthetic pyrethroid insecticide, used to control pests in domestic, industrial and agricultural environments. According to recent reports, it is one of the most common contaminants in freshwater aquatic systems. The aim of this study was to evaluate its potential genotoxic effect and the activation of the superoxide dismutase (SOD) and catalase (CAT) systems of adult zebrafish gill cells after in vivo exposure. The comet assay (CA) demonstrated that gill cells are sensitive to DNA damage after in vitro exposure to hydrogen peroxide (H 2 O 2 ), showing a dose-dependent response. We also found an increase in DNA damage of gill cells following a dose- and time-dependent treatment with CYP. Moreover, it was verified that SOD and CAT activities significantly increased after exposure to 0.6 µg/L CYP, both during six and nine days. The same treatment caused a significant up-regulation of the mRNA levels of Mn-sod and cat genes. These data indicate that CYP causes gill cell´s DNA damage and oxidative stress, modifying the activities of the enzymes responsible for maintaining ROS balance, as well as in their corresponding gene expression levels.
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral; Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Casco, Victor Hugo. Ibb-conicet-uner; Argentina. Universidad Nacional de Entre Ríos; Argentina
description Cypermethrin (CYP) is a synthetic pyrethroid insecticide, used to control pests in domestic, industrial and agricultural environments. According to recent reports, it is one of the most common contaminants in freshwater aquatic systems. The aim of this study was to evaluate its potential genotoxic effect and the activation of the superoxide dismutase (SOD) and catalase (CAT) systems of adult zebrafish gill cells after in vivo exposure. The comet assay (CA) demonstrated that gill cells are sensitive to DNA damage after in vitro exposure to hydrogen peroxide (H 2 O 2 ), showing a dose-dependent response. We also found an increase in DNA damage of gill cells following a dose- and time-dependent treatment with CYP. Moreover, it was verified that SOD and CAT activities significantly increased after exposure to 0.6 µg/L CYP, both during six and nine days. The same treatment caused a significant up-regulation of the mRNA levels of Mn-sod and cat genes. These data indicate that CYP causes gill cell´s DNA damage and oxidative stress, modifying the activities of the enzymes responsible for maintaining ROS balance, as well as in their corresponding gene expression levels.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111269
Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Casco, Victor Hugo; Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 173; 5-2019; 1-7
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111269
identifier_str_mv Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Casco, Victor Hugo; Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 173; 5-2019; 1-7
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2019.02.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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