Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients

Autores
Farías, Adrián Alejandro; Ré, Viviana Elizabeth; Mengarelli, Silvia Estela; Kremer, Luis; Pisano, María Belén; Allende, Luis Ramón; Nicolás, Juan Carlos; Elbarcha, Osvaldo; Contigiani de Minio, Marta Silvia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids. In this study, we investigated the relationship between the detection of HCV RNA in body fluids (saliva, cervical smears, seminal fluid and peripheral blood mononuclear cells) from chronically HCV-infected patients and several viral and host factors. METHODOLOGY: This study comprised 16 HIV/HCV coinfected and 21 HCV monoinfected patients with a median age of 38 and 45 years, respectively. HCV-RNA was detected in serum and fluids samples by reverse transcription-nested polymerase chain reaction. Genotypes were determined by using RFLP and direct nucleotide sequencing of the PCR products and plasma viral loads by using NASBA HCV-QT. RESULTS: When compared on the basis of the results of the detection of HCV-RNA in fluids, patients did not differ significantly in relation to viral load, genotype, HCV-HIV coinfection, HCV/HIV coinfection and epidemiological host factors. Our data suggest that HCV can be detected in body fluids of chronically HCV-infected patients independent of these cofactors, including circulating HCV load. Studies on HCV dynamics are needed to gain insights into nonparenteral transmission of HCV.
Fil: Farías, Adrián Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Mengarelli, Silvia Estela. Gobierno de la Provincia de Córdoba. Nuevo Hospital San Roque; Argentina
Fil: Kremer, Luis. Gobierno de la Provincia de Cordoba. Hospital de Clinicas.; Argentina
Fil: Pisano, María Belén. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Allende, Luis Ramón. Gobierno de la Provincia de Cordoba. Hospital de Clinicas.; Argentina
Fil: Nicolás, Juan Carlos. Universidad Católica de Córdoba; Argentina
Fil: Elbarcha, Osvaldo. Universidad Católica de Córdoba; Argentina
Fil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Materia
HCV
HIV/HCV coinfection
body fluids
epidemiology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189807
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189807
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patientsFarías, Adrián AlejandroRé, Viviana ElizabethMengarelli, Silvia EstelaKremer, LuisPisano, María BelénAllende, Luis RamónNicolás, Juan CarlosElbarcha, OsvaldoContigiani de Minio, Marta SilviaHCVHIV/HCV coinfectionbody fluidsepidemiologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids. In this study, we investigated the relationship between the detection of HCV RNA in body fluids (saliva, cervical smears, seminal fluid and peripheral blood mononuclear cells) from chronically HCV-infected patients and several viral and host factors. METHODOLOGY: This study comprised 16 HIV/HCV coinfected and 21 HCV monoinfected patients with a median age of 38 and 45 years, respectively. HCV-RNA was detected in serum and fluids samples by reverse transcription-nested polymerase chain reaction. Genotypes were determined by using RFLP and direct nucleotide sequencing of the PCR products and plasma viral loads by using NASBA HCV-QT. RESULTS: When compared on the basis of the results of the detection of HCV-RNA in fluids, patients did not differ significantly in relation to viral load, genotype, HCV-HIV coinfection, HCV/HIV coinfection and epidemiological host factors. Our data suggest that HCV can be detected in body fluids of chronically HCV-infected patients independent of these cofactors, including circulating HCV load. Studies on HCV dynamics are needed to gain insights into nonparenteral transmission of HCV.Fil: Farías, Adrián Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Ré, Viviana Elizabeth. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Mengarelli, Silvia Estela. Gobierno de la Provincia de Córdoba. Nuevo Hospital San Roque; ArgentinaFil: Kremer, Luis. Gobierno de la Provincia de Cordoba. Hospital de Clinicas.; ArgentinaFil: Pisano, María Belén. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Allende, Luis Ramón. Gobierno de la Provincia de Cordoba. Hospital de Clinicas.; ArgentinaFil: Nicolás, Juan Carlos. Universidad Católica de Córdoba; ArgentinaFil: Elbarcha, Osvaldo. Universidad Católica de Córdoba; ArgentinaFil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; ArgentinaH G E Update Medical Publishing S A2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189807Farías, Adrián Alejandro; Ré, Viviana Elizabeth; Mengarelli, Silvia Estela; Kremer, Luis; Pisano, María Belén; et al.; Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients; H G E Update Medical Publishing S A; Hepato-gastroenterology; 57; 98; 12-2010; 300-3040172-6390CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/20583431/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:08Zoai:ri.conicet.gov.ar:11336/189807instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:08.725CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
spellingShingle Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
Farías, Adrián Alejandro
HCV
HIV/HCV coinfection
body fluids
epidemiology
title_short Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_full Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_fullStr Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_full_unstemmed Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_sort Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
dc.creator.none.fl_str_mv Farías, Adrián Alejandro
Ré, Viviana Elizabeth
Mengarelli, Silvia Estela
Kremer, Luis
Pisano, María Belén
Allende, Luis Ramón
Nicolás, Juan Carlos
Elbarcha, Osvaldo
Contigiani de Minio, Marta Silvia
author Farías, Adrián Alejandro
author_facet Farías, Adrián Alejandro
Ré, Viviana Elizabeth
Mengarelli, Silvia Estela
Kremer, Luis
Pisano, María Belén
Allende, Luis Ramón
Nicolás, Juan Carlos
Elbarcha, Osvaldo
Contigiani de Minio, Marta Silvia
author_role author
author2 Ré, Viviana Elizabeth
Mengarelli, Silvia Estela
Kremer, Luis
Pisano, María Belén
Allende, Luis Ramón
Nicolás, Juan Carlos
Elbarcha, Osvaldo
Contigiani de Minio, Marta Silvia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HCV
HIV/HCV coinfection
body fluids
epidemiology
topic HCV
HIV/HCV coinfection
body fluids
epidemiology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids. In this study, we investigated the relationship between the detection of HCV RNA in body fluids (saliva, cervical smears, seminal fluid and peripheral blood mononuclear cells) from chronically HCV-infected patients and several viral and host factors. METHODOLOGY: This study comprised 16 HIV/HCV coinfected and 21 HCV monoinfected patients with a median age of 38 and 45 years, respectively. HCV-RNA was detected in serum and fluids samples by reverse transcription-nested polymerase chain reaction. Genotypes were determined by using RFLP and direct nucleotide sequencing of the PCR products and plasma viral loads by using NASBA HCV-QT. RESULTS: When compared on the basis of the results of the detection of HCV-RNA in fluids, patients did not differ significantly in relation to viral load, genotype, HCV-HIV coinfection, HCV/HIV coinfection and epidemiological host factors. Our data suggest that HCV can be detected in body fluids of chronically HCV-infected patients independent of these cofactors, including circulating HCV load. Studies on HCV dynamics are needed to gain insights into nonparenteral transmission of HCV.
Fil: Farías, Adrián Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Mengarelli, Silvia Estela. Gobierno de la Provincia de Córdoba. Nuevo Hospital San Roque; Argentina
Fil: Kremer, Luis. Gobierno de la Provincia de Cordoba. Hospital de Clinicas.; Argentina
Fil: Pisano, María Belén. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Allende, Luis Ramón. Gobierno de la Provincia de Cordoba. Hospital de Clinicas.; Argentina
Fil: Nicolás, Juan Carlos. Universidad Católica de Córdoba; Argentina
Fil: Elbarcha, Osvaldo. Universidad Católica de Córdoba; Argentina
Fil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
description The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids. In this study, we investigated the relationship between the detection of HCV RNA in body fluids (saliva, cervical smears, seminal fluid and peripheral blood mononuclear cells) from chronically HCV-infected patients and several viral and host factors. METHODOLOGY: This study comprised 16 HIV/HCV coinfected and 21 HCV monoinfected patients with a median age of 38 and 45 years, respectively. HCV-RNA was detected in serum and fluids samples by reverse transcription-nested polymerase chain reaction. Genotypes were determined by using RFLP and direct nucleotide sequencing of the PCR products and plasma viral loads by using NASBA HCV-QT. RESULTS: When compared on the basis of the results of the detection of HCV-RNA in fluids, patients did not differ significantly in relation to viral load, genotype, HCV-HIV coinfection, HCV/HIV coinfection and epidemiological host factors. Our data suggest that HCV can be detected in body fluids of chronically HCV-infected patients independent of these cofactors, including circulating HCV load. Studies on HCV dynamics are needed to gain insights into nonparenteral transmission of HCV.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189807
Farías, Adrián Alejandro; Ré, Viviana Elizabeth; Mengarelli, Silvia Estela; Kremer, Luis; Pisano, María Belén; et al.; Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients; H G E Update Medical Publishing S A; Hepato-gastroenterology; 57; 98; 12-2010; 300-304
0172-6390
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189807
identifier_str_mv Farías, Adrián Alejandro; Ré, Viviana Elizabeth; Mengarelli, Silvia Estela; Kremer, Luis; Pisano, María Belén; et al.; Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients; H G E Update Medical Publishing S A; Hepato-gastroenterology; 57; 98; 12-2010; 300-304
0172-6390
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/20583431/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv H G E Update Medical Publishing S A
publisher.none.fl_str_mv H G E Update Medical Publishing S A
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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