The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development

Autores
Espiritu, Eugenel B.; Crunk, Amanda E.; Bais, Abha; Hochbaum, Daniel; Cervino, Ailen Soledad; Phua, Yu Leng; Butterworth, Michael B.; Goto, Toshiyasu; Ho, Jacqueline; Hukriede, Neil A.; Cirio, Maria Cecilia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The molecular events driving specification of the kidney have been well characterized. However, how the initial kidney field size is established, patterned, and proportioned is not well characterized. Lhx1 is a transcription factor expressed in pronephric progenitors and is required for specification of the kidney, but few Lhx1 interacting proteins or downstream targets have been identified. By tandem-affinity purification, we isolated FRY like transcriptional coactivator (Fryl), one of two paralogous genes, fryl and furry (fry), have been described in vertebrates. Both proteins were found to interact with the Ldb1-Lhx1 complex, but our studies focused on Lhx1/Fry functional roles, as they are expressed in overlapping domains. We found that Xenopus embryos depleted of fry exhibit loss of pronephric mesoderm, phenocopying the Lhx1-depleted animals. In addition, we demonstrated a synergism between Fry and Lhx1, identified candidate microRNAs regulated by the pair, and confirmed these microRNA clusters influence specification of the kidney. Therefore, our data shows that a constitutively-active Ldb1-Lhx1 complex interacts with a broadly expressed microRNA repressor, Fry, to establish the kidney field.
Fil: Espiritu, Eugenel B.. University of Pittsburgh; Estados Unidos
Fil: Crunk, Amanda E.. University of Pittsburgh; Estados Unidos
Fil: Bais, Abha. University of Pittsburgh; Estados Unidos
Fil: Hochbaum, Daniel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cervino, Ailen Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Phua, Yu Leng. University Of Pittsburgh Medical Center; Estados Unidos
Fil: Butterworth, Michael B.. University of Pittsburgh; Estados Unidos
Fil: Goto, Toshiyasu. Tokyo Medical And Dental University; Japón
Fil: Ho, Jacqueline. University Of Pittsburgh Medical Center; Estados Unidos
Fil: Hukriede, Neil A.. University of Pittsburgh; Estados Unidos
Fil: Cirio, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
FURRY
LHX1
MICRORNAS
KIDNEY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89280
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney developmentEspiritu, Eugenel B.Crunk, Amanda E.Bais, AbhaHochbaum, DanielCervino, Ailen SoledadPhua, Yu LengButterworth, Michael B.Goto, ToshiyasuHo, JacquelineHukriede, Neil A.Cirio, Maria CeciliaFURRYLHX1MICRORNASKIDNEYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The molecular events driving specification of the kidney have been well characterized. However, how the initial kidney field size is established, patterned, and proportioned is not well characterized. Lhx1 is a transcription factor expressed in pronephric progenitors and is required for specification of the kidney, but few Lhx1 interacting proteins or downstream targets have been identified. By tandem-affinity purification, we isolated FRY like transcriptional coactivator (Fryl), one of two paralogous genes, fryl and furry (fry), have been described in vertebrates. Both proteins were found to interact with the Ldb1-Lhx1 complex, but our studies focused on Lhx1/Fry functional roles, as they are expressed in overlapping domains. We found that Xenopus embryos depleted of fry exhibit loss of pronephric mesoderm, phenocopying the Lhx1-depleted animals. In addition, we demonstrated a synergism between Fry and Lhx1, identified candidate microRNAs regulated by the pair, and confirmed these microRNA clusters influence specification of the kidney. Therefore, our data shows that a constitutively-active Ldb1-Lhx1 complex interacts with a broadly expressed microRNA repressor, Fry, to establish the kidney field.Fil: Espiritu, Eugenel B.. University of Pittsburgh; Estados UnidosFil: Crunk, Amanda E.. University of Pittsburgh; Estados UnidosFil: Bais, Abha. University of Pittsburgh; Estados UnidosFil: Hochbaum, Daniel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cervino, Ailen Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Phua, Yu Leng. University Of Pittsburgh Medical Center; Estados UnidosFil: Butterworth, Michael B.. University of Pittsburgh; Estados UnidosFil: Goto, Toshiyasu. Tokyo Medical And Dental University; JapónFil: Ho, Jacqueline. University Of Pittsburgh Medical Center; Estados UnidosFil: Hukriede, Neil A.. University of Pittsburgh; Estados UnidosFil: Cirio, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaNature Publishing Group2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89280Espiritu, Eugenel B.; Crunk, Amanda E.; Bais, Abha; Hochbaum, Daniel; Cervino, Ailen Soledad; et al.; The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-162045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-34038-xinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-34038-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:12Zoai:ri.conicet.gov.ar:11336/89280instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:12.63CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
title The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
spellingShingle The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
Espiritu, Eugenel B.
FURRY
LHX1
MICRORNAS
KIDNEY
title_short The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
title_full The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
title_fullStr The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
title_full_unstemmed The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
title_sort The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
dc.creator.none.fl_str_mv Espiritu, Eugenel B.
Crunk, Amanda E.
Bais, Abha
Hochbaum, Daniel
Cervino, Ailen Soledad
Phua, Yu Leng
Butterworth, Michael B.
Goto, Toshiyasu
Ho, Jacqueline
Hukriede, Neil A.
Cirio, Maria Cecilia
author Espiritu, Eugenel B.
author_facet Espiritu, Eugenel B.
Crunk, Amanda E.
Bais, Abha
Hochbaum, Daniel
Cervino, Ailen Soledad
Phua, Yu Leng
Butterworth, Michael B.
Goto, Toshiyasu
Ho, Jacqueline
Hukriede, Neil A.
Cirio, Maria Cecilia
author_role author
author2 Crunk, Amanda E.
Bais, Abha
Hochbaum, Daniel
Cervino, Ailen Soledad
Phua, Yu Leng
Butterworth, Michael B.
Goto, Toshiyasu
Ho, Jacqueline
Hukriede, Neil A.
Cirio, Maria Cecilia
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FURRY
LHX1
MICRORNAS
KIDNEY
topic FURRY
LHX1
MICRORNAS
KIDNEY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The molecular events driving specification of the kidney have been well characterized. However, how the initial kidney field size is established, patterned, and proportioned is not well characterized. Lhx1 is a transcription factor expressed in pronephric progenitors and is required for specification of the kidney, but few Lhx1 interacting proteins or downstream targets have been identified. By tandem-affinity purification, we isolated FRY like transcriptional coactivator (Fryl), one of two paralogous genes, fryl and furry (fry), have been described in vertebrates. Both proteins were found to interact with the Ldb1-Lhx1 complex, but our studies focused on Lhx1/Fry functional roles, as they are expressed in overlapping domains. We found that Xenopus embryos depleted of fry exhibit loss of pronephric mesoderm, phenocopying the Lhx1-depleted animals. In addition, we demonstrated a synergism between Fry and Lhx1, identified candidate microRNAs regulated by the pair, and confirmed these microRNA clusters influence specification of the kidney. Therefore, our data shows that a constitutively-active Ldb1-Lhx1 complex interacts with a broadly expressed microRNA repressor, Fry, to establish the kidney field.
Fil: Espiritu, Eugenel B.. University of Pittsburgh; Estados Unidos
Fil: Crunk, Amanda E.. University of Pittsburgh; Estados Unidos
Fil: Bais, Abha. University of Pittsburgh; Estados Unidos
Fil: Hochbaum, Daniel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cervino, Ailen Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Phua, Yu Leng. University Of Pittsburgh Medical Center; Estados Unidos
Fil: Butterworth, Michael B.. University of Pittsburgh; Estados Unidos
Fil: Goto, Toshiyasu. Tokyo Medical And Dental University; Japón
Fil: Ho, Jacqueline. University Of Pittsburgh Medical Center; Estados Unidos
Fil: Hukriede, Neil A.. University of Pittsburgh; Estados Unidos
Fil: Cirio, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description The molecular events driving specification of the kidney have been well characterized. However, how the initial kidney field size is established, patterned, and proportioned is not well characterized. Lhx1 is a transcription factor expressed in pronephric progenitors and is required for specification of the kidney, but few Lhx1 interacting proteins or downstream targets have been identified. By tandem-affinity purification, we isolated FRY like transcriptional coactivator (Fryl), one of two paralogous genes, fryl and furry (fry), have been described in vertebrates. Both proteins were found to interact with the Ldb1-Lhx1 complex, but our studies focused on Lhx1/Fry functional roles, as they are expressed in overlapping domains. We found that Xenopus embryos depleted of fry exhibit loss of pronephric mesoderm, phenocopying the Lhx1-depleted animals. In addition, we demonstrated a synergism between Fry and Lhx1, identified candidate microRNAs regulated by the pair, and confirmed these microRNA clusters influence specification of the kidney. Therefore, our data shows that a constitutively-active Ldb1-Lhx1 complex interacts with a broadly expressed microRNA repressor, Fry, to establish the kidney field.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89280
Espiritu, Eugenel B.; Crunk, Amanda E.; Bais, Abha; Hochbaum, Daniel; Cervino, Ailen Soledad; et al.; The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89280
identifier_str_mv Espiritu, Eugenel B.; Crunk, Amanda E.; Bais, Abha; Hochbaum, Daniel; Cervino, Ailen Soledad; et al.; The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-34038-x
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-34038-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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