Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides

Autores
Garrido, Paula Melisa; Antúnez, Karina; Martin, Mariana Laura; Porrini, Martín Pablo; Zunino, Pablo; Eguaras, Martin Javier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecules strongly impact on immune signaling cascades and cellular immunity. In the present work, LC50 in six-day-old bees were determined for the following acaricides: tau-fluvalinate, flumethrin, amitraz and coumaphos. According to this obtained value, a group of individuals was treated with each acaricide and then processed for qPCR analysis. Transcript levels for genes encoding antimicrobial peptides and immune-related proteins were assessed. Flumethrin increased the expression of hymenoptaecin when comparing treated and control bees. Significant differences were recorded between coumaphos and flumethrin treatments, while the first one reduced the expression of hymenoptaecin and abaecin, the last one up-regulated their expressions. No significant statistically changes were recorded in the expression levels of vitellogenin, lysozyme or glucose dehydrogenase among bees treated with acaricides and control bees. This work constitutes the first report, under laboratory conditions, about induction of immune related genes in response to synthetic miticides.
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Martin, Mariana Laura. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Apis Mellifera
Synthetic Acaricides
Immune Related Genes
Lc50
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25140
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricidesGarrido, Paula MelisaAntúnez, KarinaMartin, Mariana LauraPorrini, Martín PabloZunino, PabloEguaras, Martin JavierApis MelliferaSynthetic AcaricidesImmune Related GenesLc50https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecules strongly impact on immune signaling cascades and cellular immunity. In the present work, LC50 in six-day-old bees were determined for the following acaricides: tau-fluvalinate, flumethrin, amitraz and coumaphos. According to this obtained value, a group of individuals was treated with each acaricide and then processed for qPCR analysis. Transcript levels for genes encoding antimicrobial peptides and immune-related proteins were assessed. Flumethrin increased the expression of hymenoptaecin when comparing treated and control bees. Significant differences were recorded between coumaphos and flumethrin treatments, while the first one reduced the expression of hymenoptaecin and abaecin, the last one up-regulated their expressions. No significant statistically changes were recorded in the expression levels of vitellogenin, lysozyme or glucose dehydrogenase among bees treated with acaricides and control bees. This work constitutes the first report, under laboratory conditions, about induction of immune related genes in response to synthetic miticides.Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Martin, Mariana Laura. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaPergamon-Elsevier Science Ltd.2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25140Garrido, Paula Melisa; Antúnez, Karina; Martin, Mariana Laura; Porrini, Martín Pablo; Zunino, Pablo; et al.; Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides; Pergamon-Elsevier Science Ltd.; Journal of Insect Physiology; 59; 1; 2-2013; 113-1190022-1910CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jinsphys.2012.10.019info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022191012002727info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:22:19Zoai:ri.conicet.gov.ar:11336/25140instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:22:20.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
title Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
spellingShingle Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
Garrido, Paula Melisa
Apis Mellifera
Synthetic Acaricides
Immune Related Genes
Lc50
title_short Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
title_full Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
title_fullStr Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
title_full_unstemmed Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
title_sort Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
dc.creator.none.fl_str_mv Garrido, Paula Melisa
Antúnez, Karina
Martin, Mariana Laura
Porrini, Martín Pablo
Zunino, Pablo
Eguaras, Martin Javier
author Garrido, Paula Melisa
author_facet Garrido, Paula Melisa
Antúnez, Karina
Martin, Mariana Laura
Porrini, Martín Pablo
Zunino, Pablo
Eguaras, Martin Javier
author_role author
author2 Antúnez, Karina
Martin, Mariana Laura
Porrini, Martín Pablo
Zunino, Pablo
Eguaras, Martin Javier
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Apis Mellifera
Synthetic Acaricides
Immune Related Genes
Lc50
topic Apis Mellifera
Synthetic Acaricides
Immune Related Genes
Lc50
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecules strongly impact on immune signaling cascades and cellular immunity. In the present work, LC50 in six-day-old bees were determined for the following acaricides: tau-fluvalinate, flumethrin, amitraz and coumaphos. According to this obtained value, a group of individuals was treated with each acaricide and then processed for qPCR analysis. Transcript levels for genes encoding antimicrobial peptides and immune-related proteins were assessed. Flumethrin increased the expression of hymenoptaecin when comparing treated and control bees. Significant differences were recorded between coumaphos and flumethrin treatments, while the first one reduced the expression of hymenoptaecin and abaecin, the last one up-regulated their expressions. No significant statistically changes were recorded in the expression levels of vitellogenin, lysozyme or glucose dehydrogenase among bees treated with acaricides and control bees. This work constitutes the first report, under laboratory conditions, about induction of immune related genes in response to synthetic miticides.
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Martin, Mariana Laura. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
description The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecules strongly impact on immune signaling cascades and cellular immunity. In the present work, LC50 in six-day-old bees were determined for the following acaricides: tau-fluvalinate, flumethrin, amitraz and coumaphos. According to this obtained value, a group of individuals was treated with each acaricide and then processed for qPCR analysis. Transcript levels for genes encoding antimicrobial peptides and immune-related proteins were assessed. Flumethrin increased the expression of hymenoptaecin when comparing treated and control bees. Significant differences were recorded between coumaphos and flumethrin treatments, while the first one reduced the expression of hymenoptaecin and abaecin, the last one up-regulated their expressions. No significant statistically changes were recorded in the expression levels of vitellogenin, lysozyme or glucose dehydrogenase among bees treated with acaricides and control bees. This work constitutes the first report, under laboratory conditions, about induction of immune related genes in response to synthetic miticides.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25140
Garrido, Paula Melisa; Antúnez, Karina; Martin, Mariana Laura; Porrini, Martín Pablo; Zunino, Pablo; et al.; Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides; Pergamon-Elsevier Science Ltd.; Journal of Insect Physiology; 59; 1; 2-2013; 113-119
0022-1910
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25140
identifier_str_mv Garrido, Paula Melisa; Antúnez, Karina; Martin, Mariana Laura; Porrini, Martín Pablo; Zunino, Pablo; et al.; Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides; Pergamon-Elsevier Science Ltd.; Journal of Insect Physiology; 59; 1; 2-2013; 113-119
0022-1910
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jinsphys.2012.10.019
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022191012002727
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd.
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082622189469696
score 13.221938

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