Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep

Autores
Pedrana, G.; Viotti, M. H.; Souza, E.; Slodoba, D.; Martin, G. B.; Cavestany, D.; Ortega, Hugo Hector
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pre-natal glucocorticoids are used in women at risk of preterm delivery to induce foetal lung maturation. However, glucocorticoids can produce negative outcomes for other tissues such as the reproductive system. We therefore tested the effects of pre-natal betamethasone on testicular morphology and apoptotic protein immune expression during pre- and post-natal development. Pregnant ewes (n = 42) bearing singleton male foetuses were randomly allocated to receive intramuscular injections of saline or betamethasone (0. 5 mg/kg) at 104, 111 and 118 days of gestation (DG). Testes were collected at 121 and 132 DG, and at 45 and 90 post-natal days (PD) and subjected to morphometric analysis (volume densities of sex cords and interstitial tissues; sex cord diameter). Immunohistochemistry (% stained area) was used to assess active caspase-3, Bax, Bcl-2 and cell-cycle proteins (PCNA). Compared with control values, betamethasone treatment decreased sex cord diameter at 121 DG, 45 and 90 PD, and sex cord volume at 90 PD. Active caspase-3 was decreased by betamethasone at 121 DG and 90 PD, but Bax was increased in all betamethasone groups. Bcl-2 and PCNA decreased in the betamethasone groups at 121 DG and 45 PD, but increased at 132 DG and 90 PD. We conclude that high levels of pre-natally administered glucocorticoid reduce foetal testicular development, perhaps via changes in the balance between pro- and anti-apoptotic proteins and cell-cycle proteins. These outcomes could compromise the future spermatogenic potential of male offspring.
Fil: Pedrana, G.. Universidad de la Republica; Uruguay
Fil: Viotti, M. H.. Universidad de la Republica; Uruguay
Fil: Souza, E.. Universidad de la Republica; Uruguay
Fil: Slodoba, D.. Mc Master University; Canadá
Fil: Martin, G. B.. University of Western Australia; Australia
Fil: Cavestany, D.. Universidad de la Republica; Uruguay
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Materia
Ovario
Pubertar
Cortisol
Ovejas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3586
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3586
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the SheepPedrana, G.Viotti, M. H.Souza, E.Slodoba, D.Martin, G. B.Cavestany, D.Ortega, Hugo HectorOvarioPubertarCortisolOvejashttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Pre-natal glucocorticoids are used in women at risk of preterm delivery to induce foetal lung maturation. However, glucocorticoids can produce negative outcomes for other tissues such as the reproductive system. We therefore tested the effects of pre-natal betamethasone on testicular morphology and apoptotic protein immune expression during pre- and post-natal development. Pregnant ewes (n = 42) bearing singleton male foetuses were randomly allocated to receive intramuscular injections of saline or betamethasone (0. 5 mg/kg) at 104, 111 and 118 days of gestation (DG). Testes were collected at 121 and 132 DG, and at 45 and 90 post-natal days (PD) and subjected to morphometric analysis (volume densities of sex cords and interstitial tissues; sex cord diameter). Immunohistochemistry (% stained area) was used to assess active caspase-3, Bax, Bcl-2 and cell-cycle proteins (PCNA). Compared with control values, betamethasone treatment decreased sex cord diameter at 121 DG, 45 and 90 PD, and sex cord volume at 90 PD. Active caspase-3 was decreased by betamethasone at 121 DG and 90 PD, but Bax was increased in all betamethasone groups. Bcl-2 and PCNA decreased in the betamethasone groups at 121 DG and 45 PD, but increased at 132 DG and 90 PD. We conclude that high levels of pre-natally administered glucocorticoid reduce foetal testicular development, perhaps via changes in the balance between pro- and anti-apoptotic proteins and cell-cycle proteins. These outcomes could compromise the future spermatogenic potential of male offspring.Fil: Pedrana, G.. Universidad de la Republica; UruguayFil: Viotti, M. H.. Universidad de la Republica; UruguayFil: Souza, E.. Universidad de la Republica; UruguayFil: Slodoba, D.. Mc Master University; CanadáFil: Martin, G. B.. University of Western Australia; AustraliaFil: Cavestany, D.. Universidad de la Republica; UruguayFil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaWiley2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3586Pedrana, G.; Viotti, M. H.; Souza, E.; Slodoba, D.; Martin, G. B.; et al.; Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep; Wiley; Reproduction in Domestic Animals; 48; 5; 5-2013; 795-8020936-6768enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12164/abstractinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/rda.12164info:eu-repo/semantics/altIdentifier/issn/0936-6768info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:40:35Zoai:ri.conicet.gov.ar:11336/3586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:40:35.991CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
title Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
spellingShingle Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
Pedrana, G.
Ovario
Pubertar
Cortisol
Ovejas
title_short Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
title_full Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
title_fullStr Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
title_full_unstemmed Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
title_sort Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep
dc.creator.none.fl_str_mv Pedrana, G.
Viotti, M. H.
Souza, E.
Slodoba, D.
Martin, G. B.
Cavestany, D.
Ortega, Hugo Hector
author Pedrana, G.
author_facet Pedrana, G.
Viotti, M. H.
Souza, E.
Slodoba, D.
Martin, G. B.
Cavestany, D.
Ortega, Hugo Hector
author_role author
author2 Viotti, M. H.
Souza, E.
Slodoba, D.
Martin, G. B.
Cavestany, D.
Ortega, Hugo Hector
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ovario
Pubertar
Cortisol
Ovejas
topic Ovario
Pubertar
Cortisol
Ovejas
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Pre-natal glucocorticoids are used in women at risk of preterm delivery to induce foetal lung maturation. However, glucocorticoids can produce negative outcomes for other tissues such as the reproductive system. We therefore tested the effects of pre-natal betamethasone on testicular morphology and apoptotic protein immune expression during pre- and post-natal development. Pregnant ewes (n = 42) bearing singleton male foetuses were randomly allocated to receive intramuscular injections of saline or betamethasone (0. 5 mg/kg) at 104, 111 and 118 days of gestation (DG). Testes were collected at 121 and 132 DG, and at 45 and 90 post-natal days (PD) and subjected to morphometric analysis (volume densities of sex cords and interstitial tissues; sex cord diameter). Immunohistochemistry (% stained area) was used to assess active caspase-3, Bax, Bcl-2 and cell-cycle proteins (PCNA). Compared with control values, betamethasone treatment decreased sex cord diameter at 121 DG, 45 and 90 PD, and sex cord volume at 90 PD. Active caspase-3 was decreased by betamethasone at 121 DG and 90 PD, but Bax was increased in all betamethasone groups. Bcl-2 and PCNA decreased in the betamethasone groups at 121 DG and 45 PD, but increased at 132 DG and 90 PD. We conclude that high levels of pre-natally administered glucocorticoid reduce foetal testicular development, perhaps via changes in the balance between pro- and anti-apoptotic proteins and cell-cycle proteins. These outcomes could compromise the future spermatogenic potential of male offspring.
Fil: Pedrana, G.. Universidad de la Republica; Uruguay
Fil: Viotti, M. H.. Universidad de la Republica; Uruguay
Fil: Souza, E.. Universidad de la Republica; Uruguay
Fil: Slodoba, D.. Mc Master University; Canadá
Fil: Martin, G. B.. University of Western Australia; Australia
Fil: Cavestany, D.. Universidad de la Republica; Uruguay
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
description Pre-natal glucocorticoids are used in women at risk of preterm delivery to induce foetal lung maturation. However, glucocorticoids can produce negative outcomes for other tissues such as the reproductive system. We therefore tested the effects of pre-natal betamethasone on testicular morphology and apoptotic protein immune expression during pre- and post-natal development. Pregnant ewes (n = 42) bearing singleton male foetuses were randomly allocated to receive intramuscular injections of saline or betamethasone (0. 5 mg/kg) at 104, 111 and 118 days of gestation (DG). Testes were collected at 121 and 132 DG, and at 45 and 90 post-natal days (PD) and subjected to morphometric analysis (volume densities of sex cords and interstitial tissues; sex cord diameter). Immunohistochemistry (% stained area) was used to assess active caspase-3, Bax, Bcl-2 and cell-cycle proteins (PCNA). Compared with control values, betamethasone treatment decreased sex cord diameter at 121 DG, 45 and 90 PD, and sex cord volume at 90 PD. Active caspase-3 was decreased by betamethasone at 121 DG and 90 PD, but Bax was increased in all betamethasone groups. Bcl-2 and PCNA decreased in the betamethasone groups at 121 DG and 45 PD, but increased at 132 DG and 90 PD. We conclude that high levels of pre-natally administered glucocorticoid reduce foetal testicular development, perhaps via changes in the balance between pro- and anti-apoptotic proteins and cell-cycle proteins. These outcomes could compromise the future spermatogenic potential of male offspring.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3586
Pedrana, G.; Viotti, M. H.; Souza, E.; Slodoba, D.; Martin, G. B.; et al.; Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep; Wiley; Reproduction in Domestic Animals; 48; 5; 5-2013; 795-802
0936-6768
url http://hdl.handle.net/11336/3586
identifier_str_mv Pedrana, G.; Viotti, M. H.; Souza, E.; Slodoba, D.; Martin, G. B.; et al.; Apoptosis-Related Protein Expression During Pre- and Post-Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep; Wiley; Reproduction in Domestic Animals; 48; 5; 5-2013; 795-802
0936-6768
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12164/abstract
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/rda.12164
info:eu-repo/semantics/altIdentifier/issn/0936-6768
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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