Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells?
- Autores
- Perez, Pablo Fernando; Doré, Joel; Leclerc, Marion; Levenez, Florence; Benyacoub, Jalil; Serrant, Patrick; Segura Roggero, Iris; Schiffrin, Eduardo J.; Donnet Hughes, Anne
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- OBJECTIVE. We examined the presence of a natural bacterial inoculum in breast milk and its intracellular transport from the maternal intestine to the breast through the circulation. METHODS. Breast milk and peripheral blood were collected aseptically from healthy donors at various times after delivery, and the presence of viable bacteria was determined through plating. Temporal temperature gradient gel electrophoresis was used to examine the bacterial ribosomal DNA content in milk cells, maternal peripheral blood mononuclear cells, and feces and in corresponding infant feces. Blood from nongravid nonlactating women served as control samples. Bacterial translocation to extraintestinal tissues was also evaluated in virgin, pregnant, and lactating mice. RESULTS. Breast milk contained a low total concentration of microbes of <103 colony-forming units per mL. Temporal temperature gradient gel electrophoresis revealed that maternal blood and milk cells contained the genetic material of a greater biodiversity of enteric bacteria. Some bacterial signatures were common to infant feces and to samples of maternal origin. Bacterial translocation from the gut to mesenteric lymph nodes and mammary gland occurred during late pregnancy and lactation in mice. CONCLUSIONS. Bacterial translocation is a unique physiologic event, which is increased during pregnancy and lactation in rodents. Human breast milk cells contain a limited number of viable bacteria but a range of bacterial DNA signatures, as also found in maternal peripheral blood mononuclear cells. Those peripheral blood mononuclear cells showed greater biodiversity than did peripheral blood mononuclear cells from control women. Taken together, our results suggest that intestinally derived bacterial components are transported to the lactating breast within mononuclear cells. We speculate that this programs the neonatal immune system to recognize specific bacterial molecular patterns and to respond appropriately to pathogens and commensal organisms.
Fil: Perez, Pablo Fernando. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Doré, Joel. Nestlé Research Centre. Nestec; Francia
Fil: Leclerc, Marion. National Institute for Agronomic Research. Unit for Ecology and Physiology of the Digestive Tract; Francia
Fil: Levenez, Florence. National Institute for Agronomic Research. Unit for Ecology and Physiology of the Digestive Tract; Francia
Fil: Benyacoub, Jalil. Nestlé Research Centre. Nestec; Francia
Fil: Serrant, Patrick. Nestlé Research Centre. Nestec; Francia
Fil: Segura Roggero, Iris. Nestlé Research Centre. Nestec; Francia
Fil: Schiffrin, Eduardo J.. Nestlé Research Centre. Nestec; Francia
Fil: Donnet Hughes, Anne. Nestlé Research Centre. Nestec; Francia - Materia
-
Bacterial translocation
Breast milk
Immunity
Maternal and child health
Lactation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/110456
Ver los metadatos del registro completo
id |
CONICETDig_3297b827cdb63110a80a1f41820da4d2 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/110456 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells?Perez, Pablo FernandoDoré, JoelLeclerc, MarionLevenez, FlorenceBenyacoub, JalilSerrant, PatrickSegura Roggero, IrisSchiffrin, Eduardo J.Donnet Hughes, AnneBacterial translocationBreast milkImmunityMaternal and child healthLactationhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2OBJECTIVE. We examined the presence of a natural bacterial inoculum in breast milk and its intracellular transport from the maternal intestine to the breast through the circulation. METHODS. Breast milk and peripheral blood were collected aseptically from healthy donors at various times after delivery, and the presence of viable bacteria was determined through plating. Temporal temperature gradient gel electrophoresis was used to examine the bacterial ribosomal DNA content in milk cells, maternal peripheral blood mononuclear cells, and feces and in corresponding infant feces. Blood from nongravid nonlactating women served as control samples. Bacterial translocation to extraintestinal tissues was also evaluated in virgin, pregnant, and lactating mice. RESULTS. Breast milk contained a low total concentration of microbes of <103 colony-forming units per mL. Temporal temperature gradient gel electrophoresis revealed that maternal blood and milk cells contained the genetic material of a greater biodiversity of enteric bacteria. Some bacterial signatures were common to infant feces and to samples of maternal origin. Bacterial translocation from the gut to mesenteric lymph nodes and mammary gland occurred during late pregnancy and lactation in mice. CONCLUSIONS. Bacterial translocation is a unique physiologic event, which is increased during pregnancy and lactation in rodents. Human breast milk cells contain a limited number of viable bacteria but a range of bacterial DNA signatures, as also found in maternal peripheral blood mononuclear cells. Those peripheral blood mononuclear cells showed greater biodiversity than did peripheral blood mononuclear cells from control women. Taken together, our results suggest that intestinally derived bacterial components are transported to the lactating breast within mononuclear cells. We speculate that this programs the neonatal immune system to recognize specific bacterial molecular patterns and to respond appropriately to pathogens and commensal organisms.Fil: Perez, Pablo Fernando. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Doré, Joel. Nestlé Research Centre. Nestec; FranciaFil: Leclerc, Marion. National Institute for Agronomic Research. Unit for Ecology and Physiology of the Digestive Tract; FranciaFil: Levenez, Florence. National Institute for Agronomic Research. Unit for Ecology and Physiology of the Digestive Tract; FranciaFil: Benyacoub, Jalil. Nestlé Research Centre. Nestec; FranciaFil: Serrant, Patrick. Nestlé Research Centre. Nestec; FranciaFil: Segura Roggero, Iris. Nestlé Research Centre. Nestec; FranciaFil: Schiffrin, Eduardo J.. Nestlé Research Centre. Nestec; FranciaFil: Donnet Hughes, Anne. Nestlé Research Centre. Nestec; FranciaAmerican Academy of Pediatrics2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110456Perez, Pablo Fernando; Doré, Joel; Leclerc, Marion; Levenez, Florence; Benyacoub, Jalil; et al.; Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells?; American Academy of Pediatrics; Pediatrics; 119; 3; 6-2007; e724-e7320031-40051098-4275CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pediatrics.aappublications.org/content/119/3/e724info:eu-repo/semantics/altIdentifier/doi/10.1542/peds.2006-1649info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:51Zoai:ri.conicet.gov.ar:11336/110456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:51.719CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? |
title |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? |
spellingShingle |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? Perez, Pablo Fernando Bacterial translocation Breast milk Immunity Maternal and child health Lactation |
title_short |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? |
title_full |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? |
title_fullStr |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? |
title_full_unstemmed |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? |
title_sort |
Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells? |
dc.creator.none.fl_str_mv |
Perez, Pablo Fernando Doré, Joel Leclerc, Marion Levenez, Florence Benyacoub, Jalil Serrant, Patrick Segura Roggero, Iris Schiffrin, Eduardo J. Donnet Hughes, Anne |
author |
Perez, Pablo Fernando |
author_facet |
Perez, Pablo Fernando Doré, Joel Leclerc, Marion Levenez, Florence Benyacoub, Jalil Serrant, Patrick Segura Roggero, Iris Schiffrin, Eduardo J. Donnet Hughes, Anne |
author_role |
author |
author2 |
Doré, Joel Leclerc, Marion Levenez, Florence Benyacoub, Jalil Serrant, Patrick Segura Roggero, Iris Schiffrin, Eduardo J. Donnet Hughes, Anne |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Bacterial translocation Breast milk Immunity Maternal and child health Lactation |
topic |
Bacterial translocation Breast milk Immunity Maternal and child health Lactation |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
OBJECTIVE. We examined the presence of a natural bacterial inoculum in breast milk and its intracellular transport from the maternal intestine to the breast through the circulation. METHODS. Breast milk and peripheral blood were collected aseptically from healthy donors at various times after delivery, and the presence of viable bacteria was determined through plating. Temporal temperature gradient gel electrophoresis was used to examine the bacterial ribosomal DNA content in milk cells, maternal peripheral blood mononuclear cells, and feces and in corresponding infant feces. Blood from nongravid nonlactating women served as control samples. Bacterial translocation to extraintestinal tissues was also evaluated in virgin, pregnant, and lactating mice. RESULTS. Breast milk contained a low total concentration of microbes of <103 colony-forming units per mL. Temporal temperature gradient gel electrophoresis revealed that maternal blood and milk cells contained the genetic material of a greater biodiversity of enteric bacteria. Some bacterial signatures were common to infant feces and to samples of maternal origin. Bacterial translocation from the gut to mesenteric lymph nodes and mammary gland occurred during late pregnancy and lactation in mice. CONCLUSIONS. Bacterial translocation is a unique physiologic event, which is increased during pregnancy and lactation in rodents. Human breast milk cells contain a limited number of viable bacteria but a range of bacterial DNA signatures, as also found in maternal peripheral blood mononuclear cells. Those peripheral blood mononuclear cells showed greater biodiversity than did peripheral blood mononuclear cells from control women. Taken together, our results suggest that intestinally derived bacterial components are transported to the lactating breast within mononuclear cells. We speculate that this programs the neonatal immune system to recognize specific bacterial molecular patterns and to respond appropriately to pathogens and commensal organisms. Fil: Perez, Pablo Fernando. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Doré, Joel. Nestlé Research Centre. Nestec; Francia Fil: Leclerc, Marion. National Institute for Agronomic Research. Unit for Ecology and Physiology of the Digestive Tract; Francia Fil: Levenez, Florence. National Institute for Agronomic Research. Unit for Ecology and Physiology of the Digestive Tract; Francia Fil: Benyacoub, Jalil. Nestlé Research Centre. Nestec; Francia Fil: Serrant, Patrick. Nestlé Research Centre. Nestec; Francia Fil: Segura Roggero, Iris. Nestlé Research Centre. Nestec; Francia Fil: Schiffrin, Eduardo J.. Nestlé Research Centre. Nestec; Francia Fil: Donnet Hughes, Anne. Nestlé Research Centre. Nestec; Francia |
description |
OBJECTIVE. We examined the presence of a natural bacterial inoculum in breast milk and its intracellular transport from the maternal intestine to the breast through the circulation. METHODS. Breast milk and peripheral blood were collected aseptically from healthy donors at various times after delivery, and the presence of viable bacteria was determined through plating. Temporal temperature gradient gel electrophoresis was used to examine the bacterial ribosomal DNA content in milk cells, maternal peripheral blood mononuclear cells, and feces and in corresponding infant feces. Blood from nongravid nonlactating women served as control samples. Bacterial translocation to extraintestinal tissues was also evaluated in virgin, pregnant, and lactating mice. RESULTS. Breast milk contained a low total concentration of microbes of <103 colony-forming units per mL. Temporal temperature gradient gel electrophoresis revealed that maternal blood and milk cells contained the genetic material of a greater biodiversity of enteric bacteria. Some bacterial signatures were common to infant feces and to samples of maternal origin. Bacterial translocation from the gut to mesenteric lymph nodes and mammary gland occurred during late pregnancy and lactation in mice. CONCLUSIONS. Bacterial translocation is a unique physiologic event, which is increased during pregnancy and lactation in rodents. Human breast milk cells contain a limited number of viable bacteria but a range of bacterial DNA signatures, as also found in maternal peripheral blood mononuclear cells. Those peripheral blood mononuclear cells showed greater biodiversity than did peripheral blood mononuclear cells from control women. Taken together, our results suggest that intestinally derived bacterial components are transported to the lactating breast within mononuclear cells. We speculate that this programs the neonatal immune system to recognize specific bacterial molecular patterns and to respond appropriately to pathogens and commensal organisms. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/110456 Perez, Pablo Fernando; Doré, Joel; Leclerc, Marion; Levenez, Florence; Benyacoub, Jalil; et al.; Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells?; American Academy of Pediatrics; Pediatrics; 119; 3; 6-2007; e724-e732 0031-4005 1098-4275 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/110456 |
identifier_str_mv |
Perez, Pablo Fernando; Doré, Joel; Leclerc, Marion; Levenez, Florence; Benyacoub, Jalil; et al.; Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells?; American Academy of Pediatrics; Pediatrics; 119; 3; 6-2007; e724-e732 0031-4005 1098-4275 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://pediatrics.aappublications.org/content/119/3/e724 info:eu-repo/semantics/altIdentifier/doi/10.1542/peds.2006-1649 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Academy of Pediatrics |
publisher.none.fl_str_mv |
American Academy of Pediatrics |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613194612473856 |
score |
13.070432 |