Bioprospecting of the probiotic potential of yeasts isolated from a wine environment

Autores
Vergara Alvarez, Silvia Cristina; Leiva Alaniz, María José; Mestre Furlani, María Victoria; Vazquez, Fabio; Mancha Agresti, Pamela; Cristina Nally, María; Maturano, Yolanda Paola
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Autochthonous yeasts of oenological origin are adapted to highly stressful and selective environments, which makes them potential candidates for probiotics. The objective of the present study was to explore the probiotic potential of 96 native yeasts of oenological origin, their biosafety, resistance to gastrointestinal tract conditions and adhesion properties. Regarding biosafety, 66 isolates shown negative hemolytic activity, negative urease activity and susceptibility to 3 or more antifungals. After the gastrointestinal resistance test, 15 isolates were selected that showed growth at different temperatures, tolerance to low pH and the presence of bile salts in in vitro tests. In general, survival after simulated conditions of the gastrointestinal tract was high and more restrictive was the duodenal. The results of the adhesion properties showed highly variable hydrophobicity and a high percentage of autoaggregation at 24 h. The maximum production of biofilm was detected in the Pichia strains. Of a total of 96 yeast strains, 15 non-Saccharomyces yeasts presented suitable properties as probiotic candidates. The native winemaking strains performed better than the reference probiotic strain, Saccharomyces cerevisiae var. boulardii CNCM I-745, which reaffirms that these strains are promising probiotic candidates and further studies are necessary to confirm their probiosis.
Fil: Vergara Alvarez, Silvia Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Leiva Alaniz, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Mestre Furlani, María Victoria. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Mancha Agresti, Pamela. Centro Federal de Educação Tecnológica; Brasil
Fil: Cristina Nally, María. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Maturano, Yolanda Paola. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Materia
ADHESION PROPERTIES
BIOSAFETY TRAITS
GASTROINTESTINAL RESISTANCE
PROBIOTIC
SIMULATED GIT CONDITION
WINE NATIVE YEASTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222867
Acceder
id CONICETDig_32960ab3a2222f5b291ed55bf140d87a
oai_identifier_str oai:ri.conicet.gov.ar:11336/222867
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioprospecting of the probiotic potential of yeasts isolated from a wine environmentVergara Alvarez, Silvia CristinaLeiva Alaniz, María JoséMestre Furlani, María VictoriaVazquez, FabioMancha Agresti, PamelaCristina Nally, MaríaMaturano, Yolanda PaolaADHESION PROPERTIESBIOSAFETY TRAITSGASTROINTESTINAL RESISTANCEPROBIOTICSIMULATED GIT CONDITIONWINE NATIVE YEASTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Autochthonous yeasts of oenological origin are adapted to highly stressful and selective environments, which makes them potential candidates for probiotics. The objective of the present study was to explore the probiotic potential of 96 native yeasts of oenological origin, their biosafety, resistance to gastrointestinal tract conditions and adhesion properties. Regarding biosafety, 66 isolates shown negative hemolytic activity, negative urease activity and susceptibility to 3 or more antifungals. After the gastrointestinal resistance test, 15 isolates were selected that showed growth at different temperatures, tolerance to low pH and the presence of bile salts in in vitro tests. In general, survival after simulated conditions of the gastrointestinal tract was high and more restrictive was the duodenal. The results of the adhesion properties showed highly variable hydrophobicity and a high percentage of autoaggregation at 24 h. The maximum production of biofilm was detected in the Pichia strains. Of a total of 96 yeast strains, 15 non-Saccharomyces yeasts presented suitable properties as probiotic candidates. The native winemaking strains performed better than the reference probiotic strain, Saccharomyces cerevisiae var. boulardii CNCM I-745, which reaffirms that these strains are promising probiotic candidates and further studies are necessary to confirm their probiosis.Fil: Vergara Alvarez, Silvia Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Leiva Alaniz, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Mestre Furlani, María Victoria. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Mancha Agresti, Pamela. Centro Federal de Educação Tecnológica; BrasilFil: Cristina Nally, María. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Maturano, Yolanda Paola. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaAcademic Press Inc Elsevier Science2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222867Vergara Alvarez, Silvia Cristina; Leiva Alaniz, María José; Mestre Furlani, María Victoria; Vazquez, Fabio; Mancha Agresti, Pamela; et al.; Bioprospecting of the probiotic potential of yeasts isolated from a wine environment; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 164; 103767; 1-2023; 1-121087-1845CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fgb.2022.103767info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1087184522001128info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:23:47Zoai:ri.conicet.gov.ar:11336/222867instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:23:47.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
title Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
spellingShingle Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
Vergara Alvarez, Silvia Cristina
ADHESION PROPERTIES
BIOSAFETY TRAITS
GASTROINTESTINAL RESISTANCE
PROBIOTIC
SIMULATED GIT CONDITION
WINE NATIVE YEASTS
title_short Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
title_full Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
title_fullStr Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
title_full_unstemmed Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
title_sort Bioprospecting of the probiotic potential of yeasts isolated from a wine environment
dc.creator.none.fl_str_mv Vergara Alvarez, Silvia Cristina
Leiva Alaniz, María José
Mestre Furlani, María Victoria
Vazquez, Fabio
Mancha Agresti, Pamela
Cristina Nally, María
Maturano, Yolanda Paola
author Vergara Alvarez, Silvia Cristina
author_facet Vergara Alvarez, Silvia Cristina
Leiva Alaniz, María José
Mestre Furlani, María Victoria
Vazquez, Fabio
Mancha Agresti, Pamela
Cristina Nally, María
Maturano, Yolanda Paola
author_role author
author2 Leiva Alaniz, María José
Mestre Furlani, María Victoria
Vazquez, Fabio
Mancha Agresti, Pamela
Cristina Nally, María
Maturano, Yolanda Paola
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ADHESION PROPERTIES
BIOSAFETY TRAITS
GASTROINTESTINAL RESISTANCE
PROBIOTIC
SIMULATED GIT CONDITION
WINE NATIVE YEASTS
topic ADHESION PROPERTIES
BIOSAFETY TRAITS
GASTROINTESTINAL RESISTANCE
PROBIOTIC
SIMULATED GIT CONDITION
WINE NATIVE YEASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Autochthonous yeasts of oenological origin are adapted to highly stressful and selective environments, which makes them potential candidates for probiotics. The objective of the present study was to explore the probiotic potential of 96 native yeasts of oenological origin, their biosafety, resistance to gastrointestinal tract conditions and adhesion properties. Regarding biosafety, 66 isolates shown negative hemolytic activity, negative urease activity and susceptibility to 3 or more antifungals. After the gastrointestinal resistance test, 15 isolates were selected that showed growth at different temperatures, tolerance to low pH and the presence of bile salts in in vitro tests. In general, survival after simulated conditions of the gastrointestinal tract was high and more restrictive was the duodenal. The results of the adhesion properties showed highly variable hydrophobicity and a high percentage of autoaggregation at 24 h. The maximum production of biofilm was detected in the Pichia strains. Of a total of 96 yeast strains, 15 non-Saccharomyces yeasts presented suitable properties as probiotic candidates. The native winemaking strains performed better than the reference probiotic strain, Saccharomyces cerevisiae var. boulardii CNCM I-745, which reaffirms that these strains are promising probiotic candidates and further studies are necessary to confirm their probiosis.
Fil: Vergara Alvarez, Silvia Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Leiva Alaniz, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Mestre Furlani, María Victoria. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Mancha Agresti, Pamela. Centro Federal de Educação Tecnológica; Brasil
Fil: Cristina Nally, María. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Maturano, Yolanda Paola. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
description Autochthonous yeasts of oenological origin are adapted to highly stressful and selective environments, which makes them potential candidates for probiotics. The objective of the present study was to explore the probiotic potential of 96 native yeasts of oenological origin, their biosafety, resistance to gastrointestinal tract conditions and adhesion properties. Regarding biosafety, 66 isolates shown negative hemolytic activity, negative urease activity and susceptibility to 3 or more antifungals. After the gastrointestinal resistance test, 15 isolates were selected that showed growth at different temperatures, tolerance to low pH and the presence of bile salts in in vitro tests. In general, survival after simulated conditions of the gastrointestinal tract was high and more restrictive was the duodenal. The results of the adhesion properties showed highly variable hydrophobicity and a high percentage of autoaggregation at 24 h. The maximum production of biofilm was detected in the Pichia strains. Of a total of 96 yeast strains, 15 non-Saccharomyces yeasts presented suitable properties as probiotic candidates. The native winemaking strains performed better than the reference probiotic strain, Saccharomyces cerevisiae var. boulardii CNCM I-745, which reaffirms that these strains are promising probiotic candidates and further studies are necessary to confirm their probiosis.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222867
Vergara Alvarez, Silvia Cristina; Leiva Alaniz, María José; Mestre Furlani, María Victoria; Vazquez, Fabio; Mancha Agresti, Pamela; et al.; Bioprospecting of the probiotic potential of yeasts isolated from a wine environment; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 164; 103767; 1-2023; 1-12
1087-1845
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222867
identifier_str_mv Vergara Alvarez, Silvia Cristina; Leiva Alaniz, María José; Mestre Furlani, María Victoria; Vazquez, Fabio; Mancha Agresti, Pamela; et al.; Bioprospecting of the probiotic potential of yeasts isolated from a wine environment; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 164; 103767; 1-2023; 1-12
1087-1845
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fgb.2022.103767
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1087184522001128
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.589754

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