Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model

Autores
Silva, Jessica Alejandra; de Gregorio, Priscilla Romina; Nader, Maria Elena Fatima
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The design of probiotic hygiene products for daily use is considered an adequate alternativefor the restoration of the vaginal microbiome, maintaining health, and/or preventing infections ofthe female urogenital tract. Most of these probiotic products are available on the world market, buttheir efficacy and safety are not sufficiently documented. One of the requirements to transfer novelprobiotic formulas/products to the productive sector is to demonstrate their innocuity and the absenceof adverse or collateral effects on the host, mainly assayed in experimental models. The inclusion ofbeneficial lactobacilli in nanofibers by electrospinning technique has shown promising applicationpossibilities, and the immobilization of Lacticaseibacillus rhamnosus CRL1332 in nanofibers withand without bioprotective substances and their characterization were previously performed by ourresearch group. In this work, the safety of the intravaginal (i.va.) administration of these functionalnanofibers in a murine experimental model was evaluated. L. rhamnosus CRL1332 immobilized indifferent nanofibers was intravaginally inoculated into mice (seven daily doses). Vaginal washeswere taken for microbiological (cultivable lactobacilli) and cytological techniques, and the vagina wasused for histological and morphological-ultrastructural evaluation. Our results demonstrated thatthe intravaginal administration of L. rhamnosus CRL1332 immobilized in nanofibers is safe in murinemodels, given the absence of an inflammatory response at the cytological and histological levels, withminor modifications at the ultrastructural level, and also related to the normal cultivable vaginalmicrobiota. On the other hand, the number of cultivable lactobacilli increased in the vagina of micereceiving L. rhamnosus CRL1332 nanofibers. The results indicate the safety of lactobacilli-functionalnanofibers and support their inclusion in the design of vaginal probiotic products to prevent/treaturogenital infections and reconstitute the women’s vaginal microbiota.
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
VAGINAL PROBIOTIC
FUNCTIONAL NANOFIBER
SAFETY
MURINE EXPERIMENTAL MODEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/219473
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental ModelSilva, Jessica Alejandrade Gregorio, Priscilla RominaNader, Maria Elena FatimaVAGINAL PROBIOTICFUNCTIONAL NANOFIBERSAFETYMURINE EXPERIMENTAL MODELhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The design of probiotic hygiene products for daily use is considered an adequate alternativefor the restoration of the vaginal microbiome, maintaining health, and/or preventing infections ofthe female urogenital tract. Most of these probiotic products are available on the world market, buttheir efficacy and safety are not sufficiently documented. One of the requirements to transfer novelprobiotic formulas/products to the productive sector is to demonstrate their innocuity and the absenceof adverse or collateral effects on the host, mainly assayed in experimental models. The inclusion ofbeneficial lactobacilli in nanofibers by electrospinning technique has shown promising applicationpossibilities, and the immobilization of Lacticaseibacillus rhamnosus CRL1332 in nanofibers withand without bioprotective substances and their characterization were previously performed by ourresearch group. In this work, the safety of the intravaginal (i.va.) administration of these functionalnanofibers in a murine experimental model was evaluated. L. rhamnosus CRL1332 immobilized indifferent nanofibers was intravaginally inoculated into mice (seven daily doses). Vaginal washeswere taken for microbiological (cultivable lactobacilli) and cytological techniques, and the vagina wasused for histological and morphological-ultrastructural evaluation. Our results demonstrated thatthe intravaginal administration of L. rhamnosus CRL1332 immobilized in nanofibers is safe in murinemodels, given the absence of an inflammatory response at the cytological and histological levels, withminor modifications at the ultrastructural level, and also related to the normal cultivable vaginalmicrobiota. On the other hand, the number of cultivable lactobacilli increased in the vagina of micereceiving L. rhamnosus CRL1332 nanofibers. The results indicate the safety of lactobacilli-functionalnanofibers and support their inclusion in the design of vaginal probiotic products to prevent/treaturogenital infections and reconstitute the women’s vaginal microbiota.Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMDPI2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/219473Silva, Jessica Alejandra; de Gregorio, Priscilla Romina; Nader, Maria Elena Fatima; Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model; MDPI; Applied Microbiology; 3; 3; 8-2023; 1013-10262673-8007CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/applmicrobiol3030069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:54Zoai:ri.conicet.gov.ar:11336/219473instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:55.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
title Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
spellingShingle Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
Silva, Jessica Alejandra
VAGINAL PROBIOTIC
FUNCTIONAL NANOFIBER
SAFETY
MURINE EXPERIMENTAL MODEL
title_short Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
title_full Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
title_fullStr Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
title_full_unstemmed Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
title_sort Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
dc.creator.none.fl_str_mv Silva, Jessica Alejandra
de Gregorio, Priscilla Romina
Nader, Maria Elena Fatima
author Silva, Jessica Alejandra
author_facet Silva, Jessica Alejandra
de Gregorio, Priscilla Romina
Nader, Maria Elena Fatima
author_role author
author2 de Gregorio, Priscilla Romina
Nader, Maria Elena Fatima
author2_role author
author
dc.subject.none.fl_str_mv VAGINAL PROBIOTIC
FUNCTIONAL NANOFIBER
SAFETY
MURINE EXPERIMENTAL MODEL
topic VAGINAL PROBIOTIC
FUNCTIONAL NANOFIBER
SAFETY
MURINE EXPERIMENTAL MODEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The design of probiotic hygiene products for daily use is considered an adequate alternativefor the restoration of the vaginal microbiome, maintaining health, and/or preventing infections ofthe female urogenital tract. Most of these probiotic products are available on the world market, buttheir efficacy and safety are not sufficiently documented. One of the requirements to transfer novelprobiotic formulas/products to the productive sector is to demonstrate their innocuity and the absenceof adverse or collateral effects on the host, mainly assayed in experimental models. The inclusion ofbeneficial lactobacilli in nanofibers by electrospinning technique has shown promising applicationpossibilities, and the immobilization of Lacticaseibacillus rhamnosus CRL1332 in nanofibers withand without bioprotective substances and their characterization were previously performed by ourresearch group. In this work, the safety of the intravaginal (i.va.) administration of these functionalnanofibers in a murine experimental model was evaluated. L. rhamnosus CRL1332 immobilized indifferent nanofibers was intravaginally inoculated into mice (seven daily doses). Vaginal washeswere taken for microbiological (cultivable lactobacilli) and cytological techniques, and the vagina wasused for histological and morphological-ultrastructural evaluation. Our results demonstrated thatthe intravaginal administration of L. rhamnosus CRL1332 immobilized in nanofibers is safe in murinemodels, given the absence of an inflammatory response at the cytological and histological levels, withminor modifications at the ultrastructural level, and also related to the normal cultivable vaginalmicrobiota. On the other hand, the number of cultivable lactobacilli increased in the vagina of micereceiving L. rhamnosus CRL1332 nanofibers. The results indicate the safety of lactobacilli-functionalnanofibers and support their inclusion in the design of vaginal probiotic products to prevent/treaturogenital infections and reconstitute the women’s vaginal microbiota.
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description The design of probiotic hygiene products for daily use is considered an adequate alternativefor the restoration of the vaginal microbiome, maintaining health, and/or preventing infections ofthe female urogenital tract. Most of these probiotic products are available on the world market, buttheir efficacy and safety are not sufficiently documented. One of the requirements to transfer novelprobiotic formulas/products to the productive sector is to demonstrate their innocuity and the absenceof adverse or collateral effects on the host, mainly assayed in experimental models. The inclusion ofbeneficial lactobacilli in nanofibers by electrospinning technique has shown promising applicationpossibilities, and the immobilization of Lacticaseibacillus rhamnosus CRL1332 in nanofibers withand without bioprotective substances and their characterization were previously performed by ourresearch group. In this work, the safety of the intravaginal (i.va.) administration of these functionalnanofibers in a murine experimental model was evaluated. L. rhamnosus CRL1332 immobilized indifferent nanofibers was intravaginally inoculated into mice (seven daily doses). Vaginal washeswere taken for microbiological (cultivable lactobacilli) and cytological techniques, and the vagina wasused for histological and morphological-ultrastructural evaluation. Our results demonstrated thatthe intravaginal administration of L. rhamnosus CRL1332 immobilized in nanofibers is safe in murinemodels, given the absence of an inflammatory response at the cytological and histological levels, withminor modifications at the ultrastructural level, and also related to the normal cultivable vaginalmicrobiota. On the other hand, the number of cultivable lactobacilli increased in the vagina of micereceiving L. rhamnosus CRL1332 nanofibers. The results indicate the safety of lactobacilli-functionalnanofibers and support their inclusion in the design of vaginal probiotic products to prevent/treaturogenital infections and reconstitute the women’s vaginal microbiota.
publishDate 2023
dc.date.none.fl_str_mv 2023-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/219473
Silva, Jessica Alejandra; de Gregorio, Priscilla Romina; Nader, Maria Elena Fatima; Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model; MDPI; Applied Microbiology; 3; 3; 8-2023; 1013-1026
2673-8007
CONICET Digital
CONICET
url http://hdl.handle.net/11336/219473
identifier_str_mv Silva, Jessica Alejandra; de Gregorio, Priscilla Romina; Nader, Maria Elena Fatima; Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model; MDPI; Applied Microbiology; 3; 3; 8-2023; 1013-1026
2673-8007
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/applmicrobiol3030069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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