Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome
- Autores
- Panfilio, Kristen A.; Vargas Jentzsch, Iris M.; Benoit, Joshua B.; Erezyilmaz, Deniz; Suzuki, Yuichiro; Colella, Stefano; Robertson, Hugh M.; Poelchau, Monica F.; Waterhouse, Robert M.; Ioannidis, Panagiotis; Weirauch, Matthew T.; Hughes, Daniel S. T.; Murali, Shwetha C.; Werren, John H.; Jacobs, Chris G. C.; Duncan, Elizabeth J.; Armisén, David; Vreede, Barbara M. I.; Baa-Puyoulet, Patrice; Berger, Chloé S.; Chang, Chun Che; Chao, Hsu; Chen, Mei Ju M.; Chen, Yen Ta; Childers, Christopher P.; Chipman, Ariel D.; Cridge, Andrew G.; Crumière, Antonin J. J.; Dearden, Peter K.; Didion, Elise M.; Dinh, Huyen; Doddapaneni, Harsha Vardhan; Dolan, Amanda; Dugan, Shannon; Extavour, Cassandra G.; Febvay, Gérard; Friedrich, Markus; Ginzburg, Neta; Han, Yi; Heger, Peter; Holmes, Christopher J.; Horn, Thorsten; Hsiao, Yi Min; Jennings, Emily C.; Johnston, J. Spencer; Jones, Tamsin E.; Jones, Jeffery W.; Khila, Abderrahman; Koelzer, Stefan; Kovacova, Viera; Leask, Megan; Lee, Sandra L.; Lee, Chien Yueh; Lovegrove, MacKenzie R.; Lu, Hsiao Ling; Lu, Yong; Moore, Patricia J.; Munoz-Torres, Monica C.; Muzny, Donna M.; Palli, Subba R.; Parisot, Nicolas; Pick, Leslie; Porter, Megan L.; Qu, Jiaxin; Refki, Peter N.; Richter, Rose; Rivera Pomar, Rolando; Rosendale, Andrew J.; Roth, Siegfried; Sachs, Lena; Santos, M. Emília; Seibert, Jan; Sghaier, Essia; Shukla, Jayendra N.; Stancliffe, Richard J.; Tidswell, Olivia; Traverso, Lucila María; Van Der Zee, Maurijn; Viala, Séverine; Worley, Kim C.; Zdobnov, Evgeny M.; Gibbs, Richard A.; Richards, Stephen
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: The Hemiptera (aphids, cicadas, and true bugs) are a key insect order, with high diversity for feeding ecology and excellent experimental tractability for molecular genetics. Building upon recent sequencing of hemipteran pests such as phloem-feeding aphids and blood-feeding bed bugs, we present the genome sequence and comparative analyses centered on the milkweed bug Oncopeltus fasciatus, a seed feeder of the family Lygaeidae. Results: The 926-Mb Oncopeltus genome is well represented by the current assembly and official gene set. We use our genomic and RNA-seq data not only to characterize the protein-coding gene repertoire and perform isoform-specific RNAi, but also to elucidate patterns of molecular evolution and physiology. We find ongoing, lineage-specific expansion and diversification of repressive C2H2 zinc finger proteins. The discovery of intron gain and turnover specific to the Hemiptera also prompted the evaluation of lineage and genome size as predictors of gene structure evolution. Furthermore, we identify enzymatic gains and losses that correlate with feeding biology, particularly for reductions associated with derived, fluid nutrition feeding. Conclusions: With the milkweed bug, we now have a critical mass of sequenced species for a hemimetabolous insect order and close outgroup to the Holometabola, substantially improving the diversity of insect genomics. We thereby define commonalities among the Hemiptera and delve into how hemipteran genomes reflect distinct feeding ecologies. Given Oncopeltus's strength as an experimental model, these new sequence resources bolster the foundation for molecular research and highlight technical considerations for the analysis of medium-sized invertebrate genomes.
Fil: Panfilio, Kristen A.. Universitat zu Köln; Alemania. University of Warwick; Reino Unido
Fil: Vargas Jentzsch, Iris M.. Universitat zu Köln; Alemania
Fil: Benoit, Joshua B.. University of Cincinnati; Estados Unidos
Fil: Erezyilmaz, Deniz. Stony Brook University; Estados Unidos. University of Oxford; Reino Unido
Fil: Suzuki, Yuichiro. Wellesley College; Estados Unidos
Fil: Colella, Stefano. Institut National Des Sciences Appliquées de Lyon; Francia. University of Montpellier. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia
Fil: Robertson, Hugh M.. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Poelchau, Monica F.. National Agricultural Library; Estados Unidos
Fil: Waterhouse, Robert M.. Universidad de Ginebra; Suiza. Universite de Lausanne; Suiza
Fil: Ioannidis, Panagiotis. Universidad de Ginebra; Suiza
Fil: Weirauch, Matthew T.. University of Cincinnati; Estados Unidos
Fil: Hughes, Daniel S. T.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Murali, Shwetha C.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos. University of Washington. School of Medicine; Estados Unidos. University of Washington. Howard Hughes Medical Institute; Estados Unidos
Fil: Werren, John H.. University of Rochester; Estados Unidos
Fil: Jacobs, Chris G. C.. Leiden University; Países Bajos. Instituto Max Planck Institut für Chemische Okologie; Alemania
Fil: Duncan, Elizabeth J.. University of Otago; Nueva Zelanda. University of Leeds; Reino Unido
Fil: Armisén, David. Université Claude Bernard Lyon 1; Francia
Fil: Vreede, Barbara M. I.. The Hebrew University of Jerusalem; Israel
Fil: Baa-Puyoulet, Patrice. Institut National des Sciences Appliquées de Lyon; Francia
Fil: Berger, Chloé S.. Université Claude Bernard Lyon 1; Francia
Fil: Chang, Chun Che. National Taiwan University; República de China
Fil: Chao, Hsu. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Chen, Mei Ju M.. National Agricultural Library; Estados Unidos. University of Otago; Nueva Zelanda
Fil: Chen, Yen Ta. Universitat zu Köln; Alemania
Fil: Childers, Christopher P.. National Agricultural Library; Estados Unidos
Fil: Chipman, Ariel D.. The Hebrew University of Jerusalem; Israel
Fil: Cridge, Andrew G.. University of Otago; Nueva Zelanda
Fil: Crumière, Antonin J. J.. Université Claude Bernard Lyon 1; Francia
Fil: Dearden, Peter K.. University of Otago; Nueva Zelanda
Fil: Didion, Elise M.. University of Cincinnati; Estados Unidos
Fil: Dinh, Huyen. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Doddapaneni, Harsha Vardhan. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Dolan, Amanda. University of Rochester; Estados Unidos. Rochester Institute of Technology; Estados Unidos
Fil: Dugan, Shannon. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Extavour, Cassandra G.. Harvard University; Estados Unidos
Fil: Febvay, Gérard. Institut National des Sciences Appliquées de Lyon; Francia
Fil: Friedrich, Markus. Wayne State University; Estados Unidos
Fil: Ginzburg, Neta. The Hebrew University of Jerusalem; Israel
Fil: Han, Yi. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Heger, Peter. Universitat zu Köln; Alemania
Fil: Holmes, Christopher J.. University of Cincinnati; Estados Unidos
Fil: Horn, Thorsten. Universitat zu Köln; Alemania
Fil: Hsiao, Yi Min. National Taiwan University; República de China
Fil: Jennings, Emily C.. University of Cincinnati; Estados Unidos
Fil: Johnston, J. Spencer. Texas A&M University; Estados Unidos
Fil: Jones, Tamsin E.. Harvard University; Estados Unidos
Fil: Jones, Jeffery W.. Wayne State University; Estados Unidos
Fil: Khila, Abderrahman. Université Claude Bernard Lyon 1; Francia
Fil: Koelzer, Stefan. Universitat zu Köln; Alemania
Fil: Kovacova, Viera. Universitat zu Köln; Alemania
Fil: Leask, Megan. University of Otago; Nueva Zelanda
Fil: Lee, Sandra L.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Lee, Chien Yueh. National Agricultural Library; Estados Unidos
Fil: Lovegrove, MacKenzie R.. University of Otago; Nueva Zelanda
Fil: Lu, Hsiao Ling. National Taiwan University; República de China
Fil: Lu, Yong. University of Maryland; Estados Unidos
Fil: Moore, Patricia J.. University of Georgia; Estados Unidos
Fil: Munoz-Torres, Monica C.. Lawrence Berkeley National Laboratory; Estados Unidos
Fil: Muzny, Donna M.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Palli, Subba R.. University of Kentucky; Estados Unidos
Fil: Parisot, Nicolas. Institut National Des Sciences Appliquées de Lyon; Francia
Fil: Pick, Leslie. University of Maryland; Estados Unidos
Fil: Porter, Megan L.. University of Hawaii at Manoa; Estados Unidos
Fil: Qu, Jiaxin. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Refki, Peter N.. Université Claude Bernard Lyon 1; Francia. Max Planck Institut für Evolutionsbiologie; Alemania
Fil: Richter, Rose. University of Rochester; Estados Unidos. Earthworks Institute; Estados Unidos
Fil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Bioinvestigaciones (Sede Pergamino); Argentina
Fil: Rosendale, Andrew J.. University of Cincinnati; Estados Unidos
Fil: Roth, Siegfried. Universitat zu Köln; Alemania
Fil: Sachs, Lena. Universitat zu Köln; Alemania
Fil: Santos, M. Emília. Université Claude Bernard Lyon 1; Francia
Fil: Seibert, Jan. Universitat zu Köln; Alemania
Fil: Sghaier, Essia. Université Claude Bernard Lyon 1; Francia
Fil: Shukla, Jayendra N.. University of Kentucky; Estados Unidos. Central University Of Rajasthan; India
Fil: Stancliffe, Richard J.. University of Hull; Reino Unido. Universitat Bonn; Alemania
Fil: Tidswell, Olivia. University of Cambridge; Estados Unidos. University of Otago; Nueva Zelanda
Fil: Traverso, Lucila María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Van Der Zee, Maurijn. Leiden University; Países Bajos
Fil: Viala, Séverine. Université Claude Bernard Lyon 1; Francia
Fil: Worley, Kim C.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Zdobnov, Evgeny M.. Universidad de Ginebra; Suiza
Fil: Gibbs, Richard A.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos
Fil: Richards, Stephen. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos - Materia
-
EVOLUTION OF DEVELOPMENT
GENE FAMILY EVOLUTION
GENE STRUCTURE
LATERAL GENE TRANSFER
PHYTOPHAGY
RNAI
TRANSCRIPTION FACTORS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/126091
Ver los metadatos del registro completo
| id |
CONICETDig_31d2ce56a6c9f9b8f40380a2c8696c0d |
|---|---|
| oai_identifier_str |
oai:ri.conicet.gov.ar:11336/126091 |
| network_acronym_str |
CONICETDig |
| repository_id_str |
3498 |
| network_name_str |
CONICET Digital (CONICET) |
| spelling |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genomePanfilio, Kristen A.Vargas Jentzsch, Iris M.Benoit, Joshua B.Erezyilmaz, DenizSuzuki, YuichiroColella, StefanoRobertson, Hugh M.Poelchau, Monica F.Waterhouse, Robert M.Ioannidis, PanagiotisWeirauch, Matthew T.Hughes, Daniel S. T.Murali, Shwetha C.Werren, John H.Jacobs, Chris G. C.Duncan, Elizabeth J.Armisén, DavidVreede, Barbara M. I.Baa-Puyoulet, PatriceBerger, Chloé S.Chang, Chun CheChao, HsuChen, Mei Ju M.Chen, Yen TaChilders, Christopher P.Chipman, Ariel D.Cridge, Andrew G.Crumière, Antonin J. J.Dearden, Peter K.Didion, Elise M.Dinh, HuyenDoddapaneni, Harsha VardhanDolan, AmandaDugan, ShannonExtavour, Cassandra G.Febvay, GérardFriedrich, MarkusGinzburg, NetaHan, YiHeger, PeterHolmes, Christopher J.Horn, ThorstenHsiao, Yi MinJennings, Emily C.Johnston, J. SpencerJones, Tamsin E.Jones, Jeffery W.Khila, AbderrahmanKoelzer, StefanKovacova, VieraLeask, MeganLee, Sandra L.Lee, Chien YuehLovegrove, MacKenzie R.Lu, Hsiao LingLu, YongMoore, Patricia J.Munoz-Torres, Monica C.Muzny, Donna M.Palli, Subba R.Parisot, NicolasPick, LesliePorter, Megan L.Qu, JiaxinRefki, Peter N.Richter, RoseRivera Pomar, RolandoRosendale, Andrew J.Roth, SiegfriedSachs, LenaSantos, M. EmíliaSeibert, JanSghaier, EssiaShukla, Jayendra N.Stancliffe, Richard J.Tidswell, OliviaTraverso, Lucila MaríaVan Der Zee, MaurijnViala, SéverineWorley, Kim C.Zdobnov, Evgeny M.Gibbs, Richard A.Richards, StephenEVOLUTION OF DEVELOPMENTGENE FAMILY EVOLUTIONGENE STRUCTURELATERAL GENE TRANSFERPHYTOPHAGYRNAITRANSCRIPTION FACTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: The Hemiptera (aphids, cicadas, and true bugs) are a key insect order, with high diversity for feeding ecology and excellent experimental tractability for molecular genetics. Building upon recent sequencing of hemipteran pests such as phloem-feeding aphids and blood-feeding bed bugs, we present the genome sequence and comparative analyses centered on the milkweed bug Oncopeltus fasciatus, a seed feeder of the family Lygaeidae. Results: The 926-Mb Oncopeltus genome is well represented by the current assembly and official gene set. We use our genomic and RNA-seq data not only to characterize the protein-coding gene repertoire and perform isoform-specific RNAi, but also to elucidate patterns of molecular evolution and physiology. We find ongoing, lineage-specific expansion and diversification of repressive C2H2 zinc finger proteins. The discovery of intron gain and turnover specific to the Hemiptera also prompted the evaluation of lineage and genome size as predictors of gene structure evolution. Furthermore, we identify enzymatic gains and losses that correlate with feeding biology, particularly for reductions associated with derived, fluid nutrition feeding. Conclusions: With the milkweed bug, we now have a critical mass of sequenced species for a hemimetabolous insect order and close outgroup to the Holometabola, substantially improving the diversity of insect genomics. We thereby define commonalities among the Hemiptera and delve into how hemipteran genomes reflect distinct feeding ecologies. Given Oncopeltus's strength as an experimental model, these new sequence resources bolster the foundation for molecular research and highlight technical considerations for the analysis of medium-sized invertebrate genomes.Fil: Panfilio, Kristen A.. Universitat zu Köln; Alemania. University of Warwick; Reino UnidoFil: Vargas Jentzsch, Iris M.. Universitat zu Köln; AlemaniaFil: Benoit, Joshua B.. University of Cincinnati; Estados UnidosFil: Erezyilmaz, Deniz. Stony Brook University; Estados Unidos. University of Oxford; Reino UnidoFil: Suzuki, Yuichiro. Wellesley College; Estados UnidosFil: Colella, Stefano. Institut National Des Sciences Appliquées de Lyon; Francia. University of Montpellier. Laboratoire des Symbioses Tropicales et Méditerranéennes; FranciaFil: Robertson, Hugh M.. University of Illinois. Urbana - Champaign; Estados UnidosFil: Poelchau, Monica F.. National Agricultural Library; Estados UnidosFil: Waterhouse, Robert M.. Universidad de Ginebra; Suiza. Universite de Lausanne; SuizaFil: Ioannidis, Panagiotis. Universidad de Ginebra; SuizaFil: Weirauch, Matthew T.. University of Cincinnati; Estados UnidosFil: Hughes, Daniel S. T.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Murali, Shwetha C.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos. University of Washington. School of Medicine; Estados Unidos. University of Washington. Howard Hughes Medical Institute; Estados UnidosFil: Werren, John H.. University of Rochester; Estados UnidosFil: Jacobs, Chris G. C.. Leiden University; Países Bajos. Instituto Max Planck Institut für Chemische Okologie; AlemaniaFil: Duncan, Elizabeth J.. University of Otago; Nueva Zelanda. University of Leeds; Reino UnidoFil: Armisén, David. Université Claude Bernard Lyon 1; FranciaFil: Vreede, Barbara M. I.. The Hebrew University of Jerusalem; IsraelFil: Baa-Puyoulet, Patrice. Institut National des Sciences Appliquées de Lyon; FranciaFil: Berger, Chloé S.. Université Claude Bernard Lyon 1; FranciaFil: Chang, Chun Che. National Taiwan University; República de ChinaFil: Chao, Hsu. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Chen, Mei Ju M.. National Agricultural Library; Estados Unidos. University of Otago; Nueva ZelandaFil: Chen, Yen Ta. Universitat zu Köln; AlemaniaFil: Childers, Christopher P.. National Agricultural Library; Estados UnidosFil: Chipman, Ariel D.. The Hebrew University of Jerusalem; IsraelFil: Cridge, Andrew G.. University of Otago; Nueva ZelandaFil: Crumière, Antonin J. J.. Université Claude Bernard Lyon 1; FranciaFil: Dearden, Peter K.. University of Otago; Nueva ZelandaFil: Didion, Elise M.. University of Cincinnati; Estados UnidosFil: Dinh, Huyen. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Doddapaneni, Harsha Vardhan. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Dolan, Amanda. University of Rochester; Estados Unidos. Rochester Institute of Technology; Estados UnidosFil: Dugan, Shannon. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Extavour, Cassandra G.. Harvard University; Estados UnidosFil: Febvay, Gérard. Institut National des Sciences Appliquées de Lyon; FranciaFil: Friedrich, Markus. Wayne State University; Estados UnidosFil: Ginzburg, Neta. The Hebrew University of Jerusalem; IsraelFil: Han, Yi. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Heger, Peter. Universitat zu Köln; AlemaniaFil: Holmes, Christopher J.. University of Cincinnati; Estados UnidosFil: Horn, Thorsten. Universitat zu Köln; AlemaniaFil: Hsiao, Yi Min. National Taiwan University; República de ChinaFil: Jennings, Emily C.. University of Cincinnati; Estados UnidosFil: Johnston, J. Spencer. Texas A&M University; Estados UnidosFil: Jones, Tamsin E.. Harvard University; Estados UnidosFil: Jones, Jeffery W.. Wayne State University; Estados UnidosFil: Khila, Abderrahman. Université Claude Bernard Lyon 1; FranciaFil: Koelzer, Stefan. Universitat zu Köln; AlemaniaFil: Kovacova, Viera. Universitat zu Köln; AlemaniaFil: Leask, Megan. University of Otago; Nueva ZelandaFil: Lee, Sandra L.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Lee, Chien Yueh. National Agricultural Library; Estados UnidosFil: Lovegrove, MacKenzie R.. University of Otago; Nueva ZelandaFil: Lu, Hsiao Ling. National Taiwan University; República de ChinaFil: Lu, Yong. University of Maryland; Estados UnidosFil: Moore, Patricia J.. University of Georgia; Estados UnidosFil: Munoz-Torres, Monica C.. Lawrence Berkeley National Laboratory; Estados UnidosFil: Muzny, Donna M.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Palli, Subba R.. University of Kentucky; Estados UnidosFil: Parisot, Nicolas. Institut National Des Sciences Appliquées de Lyon; FranciaFil: Pick, Leslie. University of Maryland; Estados UnidosFil: Porter, Megan L.. University of Hawaii at Manoa; Estados UnidosFil: Qu, Jiaxin. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Refki, Peter N.. Université Claude Bernard Lyon 1; Francia. Max Planck Institut für Evolutionsbiologie; AlemaniaFil: Richter, Rose. University of Rochester; Estados Unidos. Earthworks Institute; Estados UnidosFil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Bioinvestigaciones (Sede Pergamino); ArgentinaFil: Rosendale, Andrew J.. University of Cincinnati; Estados UnidosFil: Roth, Siegfried. Universitat zu Köln; AlemaniaFil: Sachs, Lena. Universitat zu Köln; AlemaniaFil: Santos, M. Emília. Université Claude Bernard Lyon 1; FranciaFil: Seibert, Jan. Universitat zu Köln; AlemaniaFil: Sghaier, Essia. Université Claude Bernard Lyon 1; FranciaFil: Shukla, Jayendra N.. University of Kentucky; Estados Unidos. Central University Of Rajasthan; IndiaFil: Stancliffe, Richard J.. University of Hull; Reino Unido. Universitat Bonn; AlemaniaFil: Tidswell, Olivia. University of Cambridge; Estados Unidos. University of Otago; Nueva ZelandaFil: Traverso, Lucila María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Van Der Zee, Maurijn. Leiden University; Países BajosFil: Viala, Séverine. Université Claude Bernard Lyon 1; FranciaFil: Worley, Kim C.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Zdobnov, Evgeny M.. Universidad de Ginebra; SuizaFil: Gibbs, Richard A.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosFil: Richards, Stephen. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados UnidosBioMed Central2019-04-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/126091Panfilio, Kristen A.; Vargas Jentzsch, Iris M.; Benoit, Joshua B.; Erezyilmaz, Deniz; Suzuki, Yuichiro; et al.; Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome; BioMed Central; Genome Biology; 20; 1; 2-4-2019; 1-261474-75961474-760XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1660-0info:eu-repo/semantics/altIdentifier/doi/10.1186/s13059-019-1660-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:51Zoai:ri.conicet.gov.ar:11336/126091instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:51.863CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome |
| title |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome |
| spellingShingle |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome Panfilio, Kristen A. EVOLUTION OF DEVELOPMENT GENE FAMILY EVOLUTION GENE STRUCTURE LATERAL GENE TRANSFER PHYTOPHAGY RNAI TRANSCRIPTION FACTORS |
| title_short |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome |
| title_full |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome |
| title_fullStr |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome |
| title_full_unstemmed |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome |
| title_sort |
Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome |
| dc.creator.none.fl_str_mv |
Panfilio, Kristen A. Vargas Jentzsch, Iris M. Benoit, Joshua B. Erezyilmaz, Deniz Suzuki, Yuichiro Colella, Stefano Robertson, Hugh M. Poelchau, Monica F. Waterhouse, Robert M. Ioannidis, Panagiotis Weirauch, Matthew T. Hughes, Daniel S. T. Murali, Shwetha C. Werren, John H. Jacobs, Chris G. C. Duncan, Elizabeth J. Armisén, David Vreede, Barbara M. I. Baa-Puyoulet, Patrice Berger, Chloé S. Chang, Chun Che Chao, Hsu Chen, Mei Ju M. Chen, Yen Ta Childers, Christopher P. Chipman, Ariel D. Cridge, Andrew G. Crumière, Antonin J. J. Dearden, Peter K. Didion, Elise M. Dinh, Huyen Doddapaneni, Harsha Vardhan Dolan, Amanda Dugan, Shannon Extavour, Cassandra G. Febvay, Gérard Friedrich, Markus Ginzburg, Neta Han, Yi Heger, Peter Holmes, Christopher J. Horn, Thorsten Hsiao, Yi Min Jennings, Emily C. Johnston, J. Spencer Jones, Tamsin E. Jones, Jeffery W. Khila, Abderrahman Koelzer, Stefan Kovacova, Viera Leask, Megan Lee, Sandra L. Lee, Chien Yueh Lovegrove, MacKenzie R. Lu, Hsiao Ling Lu, Yong Moore, Patricia J. Munoz-Torres, Monica C. Muzny, Donna M. Palli, Subba R. Parisot, Nicolas Pick, Leslie Porter, Megan L. Qu, Jiaxin Refki, Peter N. Richter, Rose Rivera Pomar, Rolando Rosendale, Andrew J. Roth, Siegfried Sachs, Lena Santos, M. Emília Seibert, Jan Sghaier, Essia Shukla, Jayendra N. Stancliffe, Richard J. Tidswell, Olivia Traverso, Lucila María Van Der Zee, Maurijn Viala, Séverine Worley, Kim C. Zdobnov, Evgeny M. Gibbs, Richard A. Richards, Stephen |
| author |
Panfilio, Kristen A. |
| author_facet |
Panfilio, Kristen A. Vargas Jentzsch, Iris M. Benoit, Joshua B. Erezyilmaz, Deniz Suzuki, Yuichiro Colella, Stefano Robertson, Hugh M. Poelchau, Monica F. Waterhouse, Robert M. Ioannidis, Panagiotis Weirauch, Matthew T. Hughes, Daniel S. T. Murali, Shwetha C. Werren, John H. Jacobs, Chris G. C. Duncan, Elizabeth J. Armisén, David Vreede, Barbara M. I. Baa-Puyoulet, Patrice Berger, Chloé S. Chang, Chun Che Chao, Hsu Chen, Mei Ju M. Chen, Yen Ta Childers, Christopher P. Chipman, Ariel D. Cridge, Andrew G. Crumière, Antonin J. J. Dearden, Peter K. Didion, Elise M. Dinh, Huyen Doddapaneni, Harsha Vardhan Dolan, Amanda Dugan, Shannon Extavour, Cassandra G. Febvay, Gérard Friedrich, Markus Ginzburg, Neta Han, Yi Heger, Peter Holmes, Christopher J. Horn, Thorsten Hsiao, Yi Min Jennings, Emily C. Johnston, J. Spencer Jones, Tamsin E. Jones, Jeffery W. Khila, Abderrahman Koelzer, Stefan Kovacova, Viera Leask, Megan Lee, Sandra L. Lee, Chien Yueh Lovegrove, MacKenzie R. Lu, Hsiao Ling Lu, Yong Moore, Patricia J. Munoz-Torres, Monica C. Muzny, Donna M. Palli, Subba R. Parisot, Nicolas Pick, Leslie Porter, Megan L. Qu, Jiaxin Refki, Peter N. Richter, Rose Rivera Pomar, Rolando Rosendale, Andrew J. Roth, Siegfried Sachs, Lena Santos, M. Emília Seibert, Jan Sghaier, Essia Shukla, Jayendra N. Stancliffe, Richard J. Tidswell, Olivia Traverso, Lucila María Van Der Zee, Maurijn Viala, Séverine Worley, Kim C. Zdobnov, Evgeny M. Gibbs, Richard A. Richards, Stephen |
| author_role |
author |
| author2 |
Vargas Jentzsch, Iris M. Benoit, Joshua B. Erezyilmaz, Deniz Suzuki, Yuichiro Colella, Stefano Robertson, Hugh M. Poelchau, Monica F. Waterhouse, Robert M. Ioannidis, Panagiotis Weirauch, Matthew T. Hughes, Daniel S. T. Murali, Shwetha C. Werren, John H. Jacobs, Chris G. C. Duncan, Elizabeth J. Armisén, David Vreede, Barbara M. I. Baa-Puyoulet, Patrice Berger, Chloé S. Chang, Chun Che Chao, Hsu Chen, Mei Ju M. Chen, Yen Ta Childers, Christopher P. Chipman, Ariel D. Cridge, Andrew G. Crumière, Antonin J. J. Dearden, Peter K. Didion, Elise M. Dinh, Huyen Doddapaneni, Harsha Vardhan Dolan, Amanda Dugan, Shannon Extavour, Cassandra G. Febvay, Gérard Friedrich, Markus Ginzburg, Neta Han, Yi Heger, Peter Holmes, Christopher J. Horn, Thorsten Hsiao, Yi Min Jennings, Emily C. Johnston, J. Spencer Jones, Tamsin E. Jones, Jeffery W. Khila, Abderrahman Koelzer, Stefan Kovacova, Viera Leask, Megan Lee, Sandra L. Lee, Chien Yueh Lovegrove, MacKenzie R. Lu, Hsiao Ling Lu, Yong Moore, Patricia J. Munoz-Torres, Monica C. Muzny, Donna M. Palli, Subba R. Parisot, Nicolas Pick, Leslie Porter, Megan L. Qu, Jiaxin Refki, Peter N. Richter, Rose Rivera Pomar, Rolando Rosendale, Andrew J. Roth, Siegfried Sachs, Lena Santos, M. Emília Seibert, Jan Sghaier, Essia Shukla, Jayendra N. Stancliffe, Richard J. Tidswell, Olivia Traverso, Lucila María Van Der Zee, Maurijn Viala, Séverine Worley, Kim C. Zdobnov, Evgeny M. Gibbs, Richard A. Richards, Stephen |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
EVOLUTION OF DEVELOPMENT GENE FAMILY EVOLUTION GENE STRUCTURE LATERAL GENE TRANSFER PHYTOPHAGY RNAI TRANSCRIPTION FACTORS |
| topic |
EVOLUTION OF DEVELOPMENT GENE FAMILY EVOLUTION GENE STRUCTURE LATERAL GENE TRANSFER PHYTOPHAGY RNAI TRANSCRIPTION FACTORS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Background: The Hemiptera (aphids, cicadas, and true bugs) are a key insect order, with high diversity for feeding ecology and excellent experimental tractability for molecular genetics. Building upon recent sequencing of hemipteran pests such as phloem-feeding aphids and blood-feeding bed bugs, we present the genome sequence and comparative analyses centered on the milkweed bug Oncopeltus fasciatus, a seed feeder of the family Lygaeidae. Results: The 926-Mb Oncopeltus genome is well represented by the current assembly and official gene set. We use our genomic and RNA-seq data not only to characterize the protein-coding gene repertoire and perform isoform-specific RNAi, but also to elucidate patterns of molecular evolution and physiology. We find ongoing, lineage-specific expansion and diversification of repressive C2H2 zinc finger proteins. The discovery of intron gain and turnover specific to the Hemiptera also prompted the evaluation of lineage and genome size as predictors of gene structure evolution. Furthermore, we identify enzymatic gains and losses that correlate with feeding biology, particularly for reductions associated with derived, fluid nutrition feeding. Conclusions: With the milkweed bug, we now have a critical mass of sequenced species for a hemimetabolous insect order and close outgroup to the Holometabola, substantially improving the diversity of insect genomics. We thereby define commonalities among the Hemiptera and delve into how hemipteran genomes reflect distinct feeding ecologies. Given Oncopeltus's strength as an experimental model, these new sequence resources bolster the foundation for molecular research and highlight technical considerations for the analysis of medium-sized invertebrate genomes. Fil: Panfilio, Kristen A.. Universitat zu Köln; Alemania. University of Warwick; Reino Unido Fil: Vargas Jentzsch, Iris M.. Universitat zu Köln; Alemania Fil: Benoit, Joshua B.. University of Cincinnati; Estados Unidos Fil: Erezyilmaz, Deniz. Stony Brook University; Estados Unidos. University of Oxford; Reino Unido Fil: Suzuki, Yuichiro. Wellesley College; Estados Unidos Fil: Colella, Stefano. Institut National Des Sciences Appliquées de Lyon; Francia. University of Montpellier. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia Fil: Robertson, Hugh M.. University of Illinois. Urbana - Champaign; Estados Unidos Fil: Poelchau, Monica F.. National Agricultural Library; Estados Unidos Fil: Waterhouse, Robert M.. Universidad de Ginebra; Suiza. Universite de Lausanne; Suiza Fil: Ioannidis, Panagiotis. Universidad de Ginebra; Suiza Fil: Weirauch, Matthew T.. University of Cincinnati; Estados Unidos Fil: Hughes, Daniel S. T.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Murali, Shwetha C.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos. University of Washington. School of Medicine; Estados Unidos. University of Washington. Howard Hughes Medical Institute; Estados Unidos Fil: Werren, John H.. University of Rochester; Estados Unidos Fil: Jacobs, Chris G. C.. Leiden University; Países Bajos. Instituto Max Planck Institut für Chemische Okologie; Alemania Fil: Duncan, Elizabeth J.. University of Otago; Nueva Zelanda. University of Leeds; Reino Unido Fil: Armisén, David. Université Claude Bernard Lyon 1; Francia Fil: Vreede, Barbara M. I.. The Hebrew University of Jerusalem; Israel Fil: Baa-Puyoulet, Patrice. Institut National des Sciences Appliquées de Lyon; Francia Fil: Berger, Chloé S.. Université Claude Bernard Lyon 1; Francia Fil: Chang, Chun Che. National Taiwan University; República de China Fil: Chao, Hsu. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Chen, Mei Ju M.. National Agricultural Library; Estados Unidos. University of Otago; Nueva Zelanda Fil: Chen, Yen Ta. Universitat zu Köln; Alemania Fil: Childers, Christopher P.. National Agricultural Library; Estados Unidos Fil: Chipman, Ariel D.. The Hebrew University of Jerusalem; Israel Fil: Cridge, Andrew G.. University of Otago; Nueva Zelanda Fil: Crumière, Antonin J. J.. Université Claude Bernard Lyon 1; Francia Fil: Dearden, Peter K.. University of Otago; Nueva Zelanda Fil: Didion, Elise M.. University of Cincinnati; Estados Unidos Fil: Dinh, Huyen. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Doddapaneni, Harsha Vardhan. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Dolan, Amanda. University of Rochester; Estados Unidos. Rochester Institute of Technology; Estados Unidos Fil: Dugan, Shannon. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Extavour, Cassandra G.. Harvard University; Estados Unidos Fil: Febvay, Gérard. Institut National des Sciences Appliquées de Lyon; Francia Fil: Friedrich, Markus. Wayne State University; Estados Unidos Fil: Ginzburg, Neta. The Hebrew University of Jerusalem; Israel Fil: Han, Yi. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Heger, Peter. Universitat zu Köln; Alemania Fil: Holmes, Christopher J.. University of Cincinnati; Estados Unidos Fil: Horn, Thorsten. Universitat zu Köln; Alemania Fil: Hsiao, Yi Min. National Taiwan University; República de China Fil: Jennings, Emily C.. University of Cincinnati; Estados Unidos Fil: Johnston, J. Spencer. Texas A&M University; Estados Unidos Fil: Jones, Tamsin E.. Harvard University; Estados Unidos Fil: Jones, Jeffery W.. Wayne State University; Estados Unidos Fil: Khila, Abderrahman. Université Claude Bernard Lyon 1; Francia Fil: Koelzer, Stefan. Universitat zu Köln; Alemania Fil: Kovacova, Viera. Universitat zu Köln; Alemania Fil: Leask, Megan. University of Otago; Nueva Zelanda Fil: Lee, Sandra L.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Lee, Chien Yueh. National Agricultural Library; Estados Unidos Fil: Lovegrove, MacKenzie R.. University of Otago; Nueva Zelanda Fil: Lu, Hsiao Ling. National Taiwan University; República de China Fil: Lu, Yong. University of Maryland; Estados Unidos Fil: Moore, Patricia J.. University of Georgia; Estados Unidos Fil: Munoz-Torres, Monica C.. Lawrence Berkeley National Laboratory; Estados Unidos Fil: Muzny, Donna M.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Palli, Subba R.. University of Kentucky; Estados Unidos Fil: Parisot, Nicolas. Institut National Des Sciences Appliquées de Lyon; Francia Fil: Pick, Leslie. University of Maryland; Estados Unidos Fil: Porter, Megan L.. University of Hawaii at Manoa; Estados Unidos Fil: Qu, Jiaxin. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Refki, Peter N.. Université Claude Bernard Lyon 1; Francia. Max Planck Institut für Evolutionsbiologie; Alemania Fil: Richter, Rose. University of Rochester; Estados Unidos. Earthworks Institute; Estados Unidos Fil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Bioinvestigaciones (Sede Pergamino); Argentina Fil: Rosendale, Andrew J.. University of Cincinnati; Estados Unidos Fil: Roth, Siegfried. Universitat zu Köln; Alemania Fil: Sachs, Lena. Universitat zu Köln; Alemania Fil: Santos, M. Emília. Université Claude Bernard Lyon 1; Francia Fil: Seibert, Jan. Universitat zu Köln; Alemania Fil: Sghaier, Essia. Université Claude Bernard Lyon 1; Francia Fil: Shukla, Jayendra N.. University of Kentucky; Estados Unidos. Central University Of Rajasthan; India Fil: Stancliffe, Richard J.. University of Hull; Reino Unido. Universitat Bonn; Alemania Fil: Tidswell, Olivia. University of Cambridge; Estados Unidos. University of Otago; Nueva Zelanda Fil: Traverso, Lucila María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina Fil: Van Der Zee, Maurijn. Leiden University; Países Bajos Fil: Viala, Séverine. Université Claude Bernard Lyon 1; Francia Fil: Worley, Kim C.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Zdobnov, Evgeny M.. Universidad de Ginebra; Suiza Fil: Gibbs, Richard A.. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos Fil: Richards, Stephen. Baylor College of Medicine. Department of Human and Molecular Genetics. Human Genome Sequencing Center; Estados Unidos |
| description |
Background: The Hemiptera (aphids, cicadas, and true bugs) are a key insect order, with high diversity for feeding ecology and excellent experimental tractability for molecular genetics. Building upon recent sequencing of hemipteran pests such as phloem-feeding aphids and blood-feeding bed bugs, we present the genome sequence and comparative analyses centered on the milkweed bug Oncopeltus fasciatus, a seed feeder of the family Lygaeidae. Results: The 926-Mb Oncopeltus genome is well represented by the current assembly and official gene set. We use our genomic and RNA-seq data not only to characterize the protein-coding gene repertoire and perform isoform-specific RNAi, but also to elucidate patterns of molecular evolution and physiology. We find ongoing, lineage-specific expansion and diversification of repressive C2H2 zinc finger proteins. The discovery of intron gain and turnover specific to the Hemiptera also prompted the evaluation of lineage and genome size as predictors of gene structure evolution. Furthermore, we identify enzymatic gains and losses that correlate with feeding biology, particularly for reductions associated with derived, fluid nutrition feeding. Conclusions: With the milkweed bug, we now have a critical mass of sequenced species for a hemimetabolous insect order and close outgroup to the Holometabola, substantially improving the diversity of insect genomics. We thereby define commonalities among the Hemiptera and delve into how hemipteran genomes reflect distinct feeding ecologies. Given Oncopeltus's strength as an experimental model, these new sequence resources bolster the foundation for molecular research and highlight technical considerations for the analysis of medium-sized invertebrate genomes. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-04-02 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/126091 Panfilio, Kristen A.; Vargas Jentzsch, Iris M.; Benoit, Joshua B.; Erezyilmaz, Deniz; Suzuki, Yuichiro; et al.; Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome; BioMed Central; Genome Biology; 20; 1; 2-4-2019; 1-26 1474-7596 1474-760X CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/126091 |
| identifier_str_mv |
Panfilio, Kristen A.; Vargas Jentzsch, Iris M.; Benoit, Joshua B.; Erezyilmaz, Deniz; Suzuki, Yuichiro; et al.; Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome; BioMed Central; Genome Biology; 20; 1; 2-4-2019; 1-26 1474-7596 1474-760X CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1660-0 info:eu-repo/semantics/altIdentifier/doi/10.1186/s13059-019-1660-0 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
| dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
| reponame_str |
CONICET Digital (CONICET) |
| collection |
CONICET Digital (CONICET) |
| instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
| _version_ |
1844614437642698752 |
| score |
13.070432 |