Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats

Autores
Interlandi, Victoria; Fontanetti, Pablo Alejandro; Ponce, Rubén Hugo; Gallará, Raquel Vivian; Centeno, Viviana Andrea
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We aimed to investigate the effect of maternal exposure to NaF on mandibular bone microarchitecture and phosphocalcic plasma parameters of the offspring. For this purpose, 10-, 15-, and 21-day-old pups (n = 6–8 per group) from two groups of mothers, control and NaF 50mg/L treated dams, were used. Plasma calcium (Ca) and phosphorus (P) levels and alkaline phosphatase activity (ALP) were measured. Fluoride concentration (F−) in bone and in stomach content was measured using potentiometry after isothermal distillation. Morphometric, histological, and histomorphometric analyses of the jaw bones were performed. Plasma Ca and P levels and ALP activity increased in 10-day and decreased in 21-day-old pups from NaF-treated mothers. Fluoride concentration in stomach content samples of 15- and 21-day-old nursing pups from mothers exposed to NaF in their drinking water was higher compared to that observed in control dam offspring. Mandibular F− content was higher in 21-day-old pups born to F−-exposed dams compared to those observed in age-matched control pups. Mandibular area increased in 21-day-old pups born to treated mothers as compared to controls. Mandibular bone volume BV/TV (%) was higher in offspring from NaF-exposed dams than in controls at all the studied times. The increase in bone volume after exposure to F− was concomitant with the increase in trabecular thickness and the decrease in trabecular separation. Altogether, our results showed that exposure to NaF during gestation and lactation increased mandibular area and bone volume of pups, with concomitant changes in phosphocalcic parameters associated with the bone modeling process.
Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina
Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina
Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
LACTATION
MANDIBULAR HISTOMORPHOMETRY
PREGNANCY
RAT
SODIUM FLUORIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210514
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/210514
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling RatsInterlandi, VictoriaFontanetti, Pablo AlejandroPonce, Rubén HugoGallará, Raquel VivianCenteno, Viviana AndreaLACTATIONMANDIBULAR HISTOMORPHOMETRYPREGNANCYRATSODIUM FLUORIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We aimed to investigate the effect of maternal exposure to NaF on mandibular bone microarchitecture and phosphocalcic plasma parameters of the offspring. For this purpose, 10-, 15-, and 21-day-old pups (n = 6–8 per group) from two groups of mothers, control and NaF 50mg/L treated dams, were used. Plasma calcium (Ca) and phosphorus (P) levels and alkaline phosphatase activity (ALP) were measured. Fluoride concentration (F−) in bone and in stomach content was measured using potentiometry after isothermal distillation. Morphometric, histological, and histomorphometric analyses of the jaw bones were performed. Plasma Ca and P levels and ALP activity increased in 10-day and decreased in 21-day-old pups from NaF-treated mothers. Fluoride concentration in stomach content samples of 15- and 21-day-old nursing pups from mothers exposed to NaF in their drinking water was higher compared to that observed in control dam offspring. Mandibular F− content was higher in 21-day-old pups born to F−-exposed dams compared to those observed in age-matched control pups. Mandibular area increased in 21-day-old pups born to treated mothers as compared to controls. Mandibular bone volume BV/TV (%) was higher in offspring from NaF-exposed dams than in controls at all the studied times. The increase in bone volume after exposure to F− was concomitant with the increase in trabecular thickness and the decrease in trabecular separation. Altogether, our results showed that exposure to NaF during gestation and lactation increased mandibular area and bone volume of pups, with concomitant changes in phosphocalcic parameters associated with the bone modeling process.Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología; ArgentinaFil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología; ArgentinaFil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaHumana Press2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210514Interlandi, Victoria; Fontanetti, Pablo Alejandro; Ponce, Rubén Hugo; Gallará, Raquel Vivian; Centeno, Viviana Andrea; Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats; Humana Press; Biological Trace Element Research; 185; 2; 2-2018; 395-4030163-4984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12011-018-1258-5info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-018-1258-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:40Zoai:ri.conicet.gov.ar:11336/210514instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:40.523CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
title Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
spellingShingle Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
Interlandi, Victoria
LACTATION
MANDIBULAR HISTOMORPHOMETRY
PREGNANCY
RAT
SODIUM FLUORIDE
title_short Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
title_full Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
title_fullStr Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
title_full_unstemmed Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
title_sort Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats
dc.creator.none.fl_str_mv Interlandi, Victoria
Fontanetti, Pablo Alejandro
Ponce, Rubén Hugo
Gallará, Raquel Vivian
Centeno, Viviana Andrea
author Interlandi, Victoria
author_facet Interlandi, Victoria
Fontanetti, Pablo Alejandro
Ponce, Rubén Hugo
Gallará, Raquel Vivian
Centeno, Viviana Andrea
author_role author
author2 Fontanetti, Pablo Alejandro
Ponce, Rubén Hugo
Gallará, Raquel Vivian
Centeno, Viviana Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv LACTATION
MANDIBULAR HISTOMORPHOMETRY
PREGNANCY
RAT
SODIUM FLUORIDE
topic LACTATION
MANDIBULAR HISTOMORPHOMETRY
PREGNANCY
RAT
SODIUM FLUORIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We aimed to investigate the effect of maternal exposure to NaF on mandibular bone microarchitecture and phosphocalcic plasma parameters of the offspring. For this purpose, 10-, 15-, and 21-day-old pups (n = 6–8 per group) from two groups of mothers, control and NaF 50mg/L treated dams, were used. Plasma calcium (Ca) and phosphorus (P) levels and alkaline phosphatase activity (ALP) were measured. Fluoride concentration (F−) in bone and in stomach content was measured using potentiometry after isothermal distillation. Morphometric, histological, and histomorphometric analyses of the jaw bones were performed. Plasma Ca and P levels and ALP activity increased in 10-day and decreased in 21-day-old pups from NaF-treated mothers. Fluoride concentration in stomach content samples of 15- and 21-day-old nursing pups from mothers exposed to NaF in their drinking water was higher compared to that observed in control dam offspring. Mandibular F− content was higher in 21-day-old pups born to F−-exposed dams compared to those observed in age-matched control pups. Mandibular area increased in 21-day-old pups born to treated mothers as compared to controls. Mandibular bone volume BV/TV (%) was higher in offspring from NaF-exposed dams than in controls at all the studied times. The increase in bone volume after exposure to F− was concomitant with the increase in trabecular thickness and the decrease in trabecular separation. Altogether, our results showed that exposure to NaF during gestation and lactation increased mandibular area and bone volume of pups, with concomitant changes in phosphocalcic parameters associated with the bone modeling process.
Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina
Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina
Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description We aimed to investigate the effect of maternal exposure to NaF on mandibular bone microarchitecture and phosphocalcic plasma parameters of the offspring. For this purpose, 10-, 15-, and 21-day-old pups (n = 6–8 per group) from two groups of mothers, control and NaF 50mg/L treated dams, were used. Plasma calcium (Ca) and phosphorus (P) levels and alkaline phosphatase activity (ALP) were measured. Fluoride concentration (F−) in bone and in stomach content was measured using potentiometry after isothermal distillation. Morphometric, histological, and histomorphometric analyses of the jaw bones were performed. Plasma Ca and P levels and ALP activity increased in 10-day and decreased in 21-day-old pups from NaF-treated mothers. Fluoride concentration in stomach content samples of 15- and 21-day-old nursing pups from mothers exposed to NaF in their drinking water was higher compared to that observed in control dam offspring. Mandibular F− content was higher in 21-day-old pups born to F−-exposed dams compared to those observed in age-matched control pups. Mandibular area increased in 21-day-old pups born to treated mothers as compared to controls. Mandibular bone volume BV/TV (%) was higher in offspring from NaF-exposed dams than in controls at all the studied times. The increase in bone volume after exposure to F− was concomitant with the increase in trabecular thickness and the decrease in trabecular separation. Altogether, our results showed that exposure to NaF during gestation and lactation increased mandibular area and bone volume of pups, with concomitant changes in phosphocalcic parameters associated with the bone modeling process.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210514
Interlandi, Victoria; Fontanetti, Pablo Alejandro; Ponce, Rubén Hugo; Gallará, Raquel Vivian; Centeno, Viviana Andrea; Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats; Humana Press; Biological Trace Element Research; 185; 2; 2-2018; 395-403
0163-4984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210514
identifier_str_mv Interlandi, Victoria; Fontanetti, Pablo Alejandro; Ponce, Rubén Hugo; Gallará, Raquel Vivian; Centeno, Viviana Andrea; Chronic Exposure to Fluoride During Gestation and Lactation Increases Mandibular Bone Volume of Suckling Rats; Humana Press; Biological Trace Element Research; 185; 2; 2-2018; 395-403
0163-4984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12011-018-1258-5
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-018-1258-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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