Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism

Autores
Hapon, María Belén; Varas, Silvia Mabel; Giménez, María Soledad; Jahn, Graciela Alma
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The profound impairment in litter growth produced by untreated maternal hypothyroidism (HypoT) may be a consequence of maternal metabolic dysfunctions affecting lactation. In this work we studied the effects of HypoT on mammary and liver lipid metabolism and its consequences on milk quality. Design: We studied the effects of prolonged 6-propyl-2-thiouracil (PTU)-induced HypoT (0.01% PTU in drinking water starting 8 days before mating until sacrifice) on milk macronutrient composition, liver and mammary lipid metabolism and content and serum lipid, and glucose and insulin concentrations in rats on days 7, 15 (L15), and 20 (L20) of lactation. Mammary and hepatic mRNA abundances of lipogenic enzymes were measured using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) on L15 and L20. Main Outcome: Milk lactose and triglycerides (TG) were reduced by HypoT, as well as mammary acetyl CoA carboxylase (ACC) activity on L15 and L20, and ACC and lipoprotein lipase (LPL) mRNA on L20. HypoT also decreased hepatic ACC activity on both days, ACC mRNA on L15 and liver [3H]H2O incorporation to TGs and TG content on L20. HypoT diminished insulinemia, increased serum total lipids, and decreased serum TGs on some or all the days of lactation studied. Conclusion: HypoT produces a drastic decrease in milk TGs; the main cause for this seems to be the decreases in liver TG synthesis and in circulating TGs, which, along with reduced mammary uptake of fatty acids caused by decreased LPL expression and possibly diminished mammary lipogenesis, result in an impaired mammary output of TGs to the milk. Thus, the impaired growth of the litters of HypoT mothers can be largely attributed to the low milk quality along with the impaired milk ejection.
Fil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; Argentina
Fil: Varas, Silvia Mabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; Argentina
Fil: Giménez, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
Hypothyroidism
Milk quality
Triglycerides syntesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149508
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/149508
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidismHapon, María BelénVaras, Silvia MabelGiménez, María SoledadJahn, Graciela AlmaHypothyroidismMilk qualityTriglycerides syntesishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: The profound impairment in litter growth produced by untreated maternal hypothyroidism (HypoT) may be a consequence of maternal metabolic dysfunctions affecting lactation. In this work we studied the effects of HypoT on mammary and liver lipid metabolism and its consequences on milk quality. Design: We studied the effects of prolonged 6-propyl-2-thiouracil (PTU)-induced HypoT (0.01% PTU in drinking water starting 8 days before mating until sacrifice) on milk macronutrient composition, liver and mammary lipid metabolism and content and serum lipid, and glucose and insulin concentrations in rats on days 7, 15 (L15), and 20 (L20) of lactation. Mammary and hepatic mRNA abundances of lipogenic enzymes were measured using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) on L15 and L20. Main Outcome: Milk lactose and triglycerides (TG) were reduced by HypoT, as well as mammary acetyl CoA carboxylase (ACC) activity on L15 and L20, and ACC and lipoprotein lipase (LPL) mRNA on L20. HypoT also decreased hepatic ACC activity on both days, ACC mRNA on L15 and liver [3H]H2O incorporation to TGs and TG content on L20. HypoT diminished insulinemia, increased serum total lipids, and decreased serum TGs on some or all the days of lactation studied. Conclusion: HypoT produces a drastic decrease in milk TGs; the main cause for this seems to be the decreases in liver TG synthesis and in circulating TGs, which, along with reduced mammary uptake of fatty acids caused by decreased LPL expression and possibly diminished mammary lipogenesis, result in an impaired mammary output of TGs to the milk. Thus, the impaired growth of the litters of HypoT mothers can be largely attributed to the low milk quality along with the impaired milk ejection.Fil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; ArgentinaFil: Varas, Silvia Mabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; ArgentinaFil: Giménez, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; ArgentinaFil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaMary Ann Liebert2007-01-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149508Hapon, María Belén; Varas, Silvia Mabel; Giménez, María Soledad; Jahn, Graciela Alma; Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism; Mary Ann Liebert; Thyroid; 17; 1; 5-1-2007; 11-181050-72561557-9077CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/thy.2005.0267info:eu-repo/semantics/altIdentifier/doi/10.1089/thy.2005.0267info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:15Zoai:ri.conicet.gov.ar:11336/149508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:15.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
title Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
spellingShingle Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
Hapon, María Belén
Hypothyroidism
Milk quality
Triglycerides syntesis
title_short Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
title_full Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
title_fullStr Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
title_full_unstemmed Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
title_sort Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism
dc.creator.none.fl_str_mv Hapon, María Belén
Varas, Silvia Mabel
Giménez, María Soledad
Jahn, Graciela Alma
author Hapon, María Belén
author_facet Hapon, María Belén
Varas, Silvia Mabel
Giménez, María Soledad
Jahn, Graciela Alma
author_role author
author2 Varas, Silvia Mabel
Giménez, María Soledad
Jahn, Graciela Alma
author2_role author
author
author
dc.subject.none.fl_str_mv Hypothyroidism
Milk quality
Triglycerides syntesis
topic Hypothyroidism
Milk quality
Triglycerides syntesis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: The profound impairment in litter growth produced by untreated maternal hypothyroidism (HypoT) may be a consequence of maternal metabolic dysfunctions affecting lactation. In this work we studied the effects of HypoT on mammary and liver lipid metabolism and its consequences on milk quality. Design: We studied the effects of prolonged 6-propyl-2-thiouracil (PTU)-induced HypoT (0.01% PTU in drinking water starting 8 days before mating until sacrifice) on milk macronutrient composition, liver and mammary lipid metabolism and content and serum lipid, and glucose and insulin concentrations in rats on days 7, 15 (L15), and 20 (L20) of lactation. Mammary and hepatic mRNA abundances of lipogenic enzymes were measured using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) on L15 and L20. Main Outcome: Milk lactose and triglycerides (TG) were reduced by HypoT, as well as mammary acetyl CoA carboxylase (ACC) activity on L15 and L20, and ACC and lipoprotein lipase (LPL) mRNA on L20. HypoT also decreased hepatic ACC activity on both days, ACC mRNA on L15 and liver [3H]H2O incorporation to TGs and TG content on L20. HypoT diminished insulinemia, increased serum total lipids, and decreased serum TGs on some or all the days of lactation studied. Conclusion: HypoT produces a drastic decrease in milk TGs; the main cause for this seems to be the decreases in liver TG synthesis and in circulating TGs, which, along with reduced mammary uptake of fatty acids caused by decreased LPL expression and possibly diminished mammary lipogenesis, result in an impaired mammary output of TGs to the milk. Thus, the impaired growth of the litters of HypoT mothers can be largely attributed to the low milk quality along with the impaired milk ejection.
Fil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; Argentina
Fil: Varas, Silvia Mabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; Argentina
Fil: Giménez, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Cátedra de Bioquímica Molecular; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Objective: The profound impairment in litter growth produced by untreated maternal hypothyroidism (HypoT) may be a consequence of maternal metabolic dysfunctions affecting lactation. In this work we studied the effects of HypoT on mammary and liver lipid metabolism and its consequences on milk quality. Design: We studied the effects of prolonged 6-propyl-2-thiouracil (PTU)-induced HypoT (0.01% PTU in drinking water starting 8 days before mating until sacrifice) on milk macronutrient composition, liver and mammary lipid metabolism and content and serum lipid, and glucose and insulin concentrations in rats on days 7, 15 (L15), and 20 (L20) of lactation. Mammary and hepatic mRNA abundances of lipogenic enzymes were measured using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) on L15 and L20. Main Outcome: Milk lactose and triglycerides (TG) were reduced by HypoT, as well as mammary acetyl CoA carboxylase (ACC) activity on L15 and L20, and ACC and lipoprotein lipase (LPL) mRNA on L20. HypoT also decreased hepatic ACC activity on both days, ACC mRNA on L15 and liver [3H]H2O incorporation to TGs and TG content on L20. HypoT diminished insulinemia, increased serum total lipids, and decreased serum TGs on some or all the days of lactation studied. Conclusion: HypoT produces a drastic decrease in milk TGs; the main cause for this seems to be the decreases in liver TG synthesis and in circulating TGs, which, along with reduced mammary uptake of fatty acids caused by decreased LPL expression and possibly diminished mammary lipogenesis, result in an impaired mammary output of TGs to the milk. Thus, the impaired growth of the litters of HypoT mothers can be largely attributed to the low milk quality along with the impaired milk ejection.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149508
Hapon, María Belén; Varas, Silvia Mabel; Giménez, María Soledad; Jahn, Graciela Alma; Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism; Mary Ann Liebert; Thyroid; 17; 1; 5-1-2007; 11-18
1050-7256
1557-9077
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149508
identifier_str_mv Hapon, María Belén; Varas, Silvia Mabel; Giménez, María Soledad; Jahn, Graciela Alma; Reduction of mammary and liver lipogenesis and alteration of milk composition during lactation in rats by hypothyroidism; Mary Ann Liebert; Thyroid; 17; 1; 5-1-2007; 11-18
1050-7256
1557-9077
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/thy.2005.0267
info:eu-repo/semantics/altIdentifier/doi/10.1089/thy.2005.0267
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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