Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene

Autores
Marliére, Newmar Pinto; Latorre Estivalis, Jose Manuel; Lorenzo, Marcelo Gustavo; Carrasco, David; Alves Silva, Juliana; Rodrigues, Juliana de Oliveira; Ferreira, Luciana de Lima; Lara, Luisa de Melo; Lowenberger, Carl; Guarneri, Alessandra Aparecida
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: As a result of evolution, the biology of triatomines must have been significantly adapted to accommodate trypanosome infection in a complex network of vector-vertebrate-parasite interactions. Arthropod-borne parasites have probably developed mechanisms, largely still unknown, to exploit the vector-vertebrate host interactions to ensure their transmission to suitable hosts. Triatomines exhibit a strong negative phototaxis and nocturnal activity, believed to be important for insect survival against its predators. Methodology/Principal Findings: In this study we quantified phototaxis and locomotion in starved fifth instar nymphs of Rhodnius prolixus infected with Trypanosoma cruzi or Trypanosoma rangeli. T. cruzi infection did not alter insect phototaxis, but induced an overall 20% decrease in the number of bug locomotory events. Furthermore, the significant differences induced by this parasite were concentrated at the beginning of the scotophase. Conversely, T. rangeli modified both behaviors, as it significantly decreased bug negative phototaxis, while it induced a 23% increase in the number of locomotory events in infected bugs. In this case, the significant effects were observed during the photophase. We also investigated the expression of Rpfor, the triatomine ortholog of the foraging gene known to modulate locomotion in other insects, and found a 4.8 fold increase for T. rangeli infected insects. Conclusions/Significance: We demonstrated for the first time that trypanosome infection modulates the locomotory activity of the invertebrate host. T. rangeli infection seems to be more broadly effective, as besides affecting the intensity of locomotion this parasite also diminished negative phototaxis and the expression of a behavior-associated gene in the triatomine vector.
Fil: Marliére, Newmar Pinto. Centro de Pesquisas René Rachou; Brasil
Fil: Latorre Estivalis, Jose Manuel. Centro de Pesquisas René Rachou; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzo, Marcelo Gustavo. Centro de Pesquisas René Rachou; Brasil
Fil: Carrasco, David. Swedish University of Agricultural Sciences; Suecia
Fil: Alves Silva, Juliana. Centro de Pesquisas René Rachou; Brasil. Universidade Federal de Minas Gerais; Brasil
Fil: Rodrigues, Juliana de Oliveira. Centro de Pesquisas René Rachou; Brasil
Fil: Ferreira, Luciana de Lima. Centro de Pesquisas René Rachou; Brasil
Fil: Lara, Luisa de Melo. Centro de Pesquisas René Rachou; Brasil
Fil: Lowenberger, Carl. University Fraser Simon; Canadá
Fil: Guarneri, Alessandra Aparecida. University Fraser Simon; Canadá. Centro de Pesquisas René Rachou; Brasil
Materia
Triatomines
Parasites
Behaviour
Foraging
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53484
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53484
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related geneMarliére, Newmar PintoLatorre Estivalis, Jose ManuelLorenzo, Marcelo GustavoCarrasco, DavidAlves Silva, JulianaRodrigues, Juliana de OliveiraFerreira, Luciana de LimaLara, Luisa de MeloLowenberger, CarlGuarneri, Alessandra AparecidaTriatominesParasitesBehaviourForaginghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: As a result of evolution, the biology of triatomines must have been significantly adapted to accommodate trypanosome infection in a complex network of vector-vertebrate-parasite interactions. Arthropod-borne parasites have probably developed mechanisms, largely still unknown, to exploit the vector-vertebrate host interactions to ensure their transmission to suitable hosts. Triatomines exhibit a strong negative phototaxis and nocturnal activity, believed to be important for insect survival against its predators. Methodology/Principal Findings: In this study we quantified phototaxis and locomotion in starved fifth instar nymphs of Rhodnius prolixus infected with Trypanosoma cruzi or Trypanosoma rangeli. T. cruzi infection did not alter insect phototaxis, but induced an overall 20% decrease in the number of bug locomotory events. Furthermore, the significant differences induced by this parasite were concentrated at the beginning of the scotophase. Conversely, T. rangeli modified both behaviors, as it significantly decreased bug negative phototaxis, while it induced a 23% increase in the number of locomotory events in infected bugs. In this case, the significant effects were observed during the photophase. We also investigated the expression of Rpfor, the triatomine ortholog of the foraging gene known to modulate locomotion in other insects, and found a 4.8 fold increase for T. rangeli infected insects. Conclusions/Significance: We demonstrated for the first time that trypanosome infection modulates the locomotory activity of the invertebrate host. T. rangeli infection seems to be more broadly effective, as besides affecting the intensity of locomotion this parasite also diminished negative phototaxis and the expression of a behavior-associated gene in the triatomine vector.Fil: Marliére, Newmar Pinto. Centro de Pesquisas René Rachou; BrasilFil: Latorre Estivalis, Jose Manuel. Centro de Pesquisas René Rachou; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lorenzo, Marcelo Gustavo. Centro de Pesquisas René Rachou; BrasilFil: Carrasco, David. Swedish University of Agricultural Sciences; SueciaFil: Alves Silva, Juliana. Centro de Pesquisas René Rachou; Brasil. Universidade Federal de Minas Gerais; BrasilFil: Rodrigues, Juliana de Oliveira. Centro de Pesquisas René Rachou; BrasilFil: Ferreira, Luciana de Lima. Centro de Pesquisas René Rachou; BrasilFil: Lara, Luisa de Melo. Centro de Pesquisas René Rachou; BrasilFil: Lowenberger, Carl. University Fraser Simon; CanadáFil: Guarneri, Alessandra Aparecida. University Fraser Simon; Canadá. Centro de Pesquisas René Rachou; BrasilPublic Library of Science2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53484Marliére, Newmar Pinto; Latorre Estivalis, Jose Manuel; Lorenzo, Marcelo Gustavo; Carrasco, David; Alves Silva, Juliana; et al.; Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene; Public Library of Science; Neglected Tropical Diseases; 9; 8; 8-2015; 1-20; e00039731935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003973info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003973info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:58Zoai:ri.conicet.gov.ar:11336/53484instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:58.452CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
title Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
spellingShingle Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
Marliére, Newmar Pinto
Triatomines
Parasites
Behaviour
Foraging
title_short Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
title_full Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
title_fullStr Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
title_full_unstemmed Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
title_sort Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene
dc.creator.none.fl_str_mv Marliére, Newmar Pinto
Latorre Estivalis, Jose Manuel
Lorenzo, Marcelo Gustavo
Carrasco, David
Alves Silva, Juliana
Rodrigues, Juliana de Oliveira
Ferreira, Luciana de Lima
Lara, Luisa de Melo
Lowenberger, Carl
Guarneri, Alessandra Aparecida
author Marliére, Newmar Pinto
author_facet Marliére, Newmar Pinto
Latorre Estivalis, Jose Manuel
Lorenzo, Marcelo Gustavo
Carrasco, David
Alves Silva, Juliana
Rodrigues, Juliana de Oliveira
Ferreira, Luciana de Lima
Lara, Luisa de Melo
Lowenberger, Carl
Guarneri, Alessandra Aparecida
author_role author
author2 Latorre Estivalis, Jose Manuel
Lorenzo, Marcelo Gustavo
Carrasco, David
Alves Silva, Juliana
Rodrigues, Juliana de Oliveira
Ferreira, Luciana de Lima
Lara, Luisa de Melo
Lowenberger, Carl
Guarneri, Alessandra Aparecida
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Triatomines
Parasites
Behaviour
Foraging
topic Triatomines
Parasites
Behaviour
Foraging
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: As a result of evolution, the biology of triatomines must have been significantly adapted to accommodate trypanosome infection in a complex network of vector-vertebrate-parasite interactions. Arthropod-borne parasites have probably developed mechanisms, largely still unknown, to exploit the vector-vertebrate host interactions to ensure their transmission to suitable hosts. Triatomines exhibit a strong negative phototaxis and nocturnal activity, believed to be important for insect survival against its predators. Methodology/Principal Findings: In this study we quantified phototaxis and locomotion in starved fifth instar nymphs of Rhodnius prolixus infected with Trypanosoma cruzi or Trypanosoma rangeli. T. cruzi infection did not alter insect phototaxis, but induced an overall 20% decrease in the number of bug locomotory events. Furthermore, the significant differences induced by this parasite were concentrated at the beginning of the scotophase. Conversely, T. rangeli modified both behaviors, as it significantly decreased bug negative phototaxis, while it induced a 23% increase in the number of locomotory events in infected bugs. In this case, the significant effects were observed during the photophase. We also investigated the expression of Rpfor, the triatomine ortholog of the foraging gene known to modulate locomotion in other insects, and found a 4.8 fold increase for T. rangeli infected insects. Conclusions/Significance: We demonstrated for the first time that trypanosome infection modulates the locomotory activity of the invertebrate host. T. rangeli infection seems to be more broadly effective, as besides affecting the intensity of locomotion this parasite also diminished negative phototaxis and the expression of a behavior-associated gene in the triatomine vector.
Fil: Marliére, Newmar Pinto. Centro de Pesquisas René Rachou; Brasil
Fil: Latorre Estivalis, Jose Manuel. Centro de Pesquisas René Rachou; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzo, Marcelo Gustavo. Centro de Pesquisas René Rachou; Brasil
Fil: Carrasco, David. Swedish University of Agricultural Sciences; Suecia
Fil: Alves Silva, Juliana. Centro de Pesquisas René Rachou; Brasil. Universidade Federal de Minas Gerais; Brasil
Fil: Rodrigues, Juliana de Oliveira. Centro de Pesquisas René Rachou; Brasil
Fil: Ferreira, Luciana de Lima. Centro de Pesquisas René Rachou; Brasil
Fil: Lara, Luisa de Melo. Centro de Pesquisas René Rachou; Brasil
Fil: Lowenberger, Carl. University Fraser Simon; Canadá
Fil: Guarneri, Alessandra Aparecida. University Fraser Simon; Canadá. Centro de Pesquisas René Rachou; Brasil
description Background: As a result of evolution, the biology of triatomines must have been significantly adapted to accommodate trypanosome infection in a complex network of vector-vertebrate-parasite interactions. Arthropod-borne parasites have probably developed mechanisms, largely still unknown, to exploit the vector-vertebrate host interactions to ensure their transmission to suitable hosts. Triatomines exhibit a strong negative phototaxis and nocturnal activity, believed to be important for insect survival against its predators. Methodology/Principal Findings: In this study we quantified phototaxis and locomotion in starved fifth instar nymphs of Rhodnius prolixus infected with Trypanosoma cruzi or Trypanosoma rangeli. T. cruzi infection did not alter insect phototaxis, but induced an overall 20% decrease in the number of bug locomotory events. Furthermore, the significant differences induced by this parasite were concentrated at the beginning of the scotophase. Conversely, T. rangeli modified both behaviors, as it significantly decreased bug negative phototaxis, while it induced a 23% increase in the number of locomotory events in infected bugs. In this case, the significant effects were observed during the photophase. We also investigated the expression of Rpfor, the triatomine ortholog of the foraging gene known to modulate locomotion in other insects, and found a 4.8 fold increase for T. rangeli infected insects. Conclusions/Significance: We demonstrated for the first time that trypanosome infection modulates the locomotory activity of the invertebrate host. T. rangeli infection seems to be more broadly effective, as besides affecting the intensity of locomotion this parasite also diminished negative phototaxis and the expression of a behavior-associated gene in the triatomine vector.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53484
Marliére, Newmar Pinto; Latorre Estivalis, Jose Manuel; Lorenzo, Marcelo Gustavo; Carrasco, David; Alves Silva, Juliana; et al.; Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene; Public Library of Science; Neglected Tropical Diseases; 9; 8; 8-2015; 1-20; e0003973
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53484
identifier_str_mv Marliére, Newmar Pinto; Latorre Estivalis, Jose Manuel; Lorenzo, Marcelo Gustavo; Carrasco, David; Alves Silva, Juliana; et al.; Trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene; Public Library of Science; Neglected Tropical Diseases; 9; 8; 8-2015; 1-20; e0003973
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003973
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003973
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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