In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

Autores
Pappalardo, Juan Sebastian; Langellotti, Cecilia Ana; Di Giacomo, Sebastián; Olivera, Valeria; Quattrocchi, Valeria; Zamorano, Patricia Ines; Hartner, William C.; Levchenko, Tatyana S.; Torchilin, Vladimir P.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dendritic cells (DC) are antigen presenting cells (APC) uniquely capable of priming naïve T cells and cross-presenting antigens and determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by an enhanced expression of the enhanced green fluorescent protein (EGFP) and bovine herpes virus type 1 (BoHV-1) glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L as indicated by the secretion of interleukin 6 (IL-6).
Fil: Pappalardo, Juan Sebastian. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Hartner, William C.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Levchenko, Tatyana S.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Torchilin, Vladimir P.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Materia
TAT peptide
Bovine herpes virus 1 glycoprotein D
Dendritic cell transfection
Green fluorescent protein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33327
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/33327
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomesPappalardo, Juan SebastianLangellotti, Cecilia AnaDi Giacomo, SebastiánOlivera, ValeriaQuattrocchi, ValeriaZamorano, Patricia InesHartner, William C.Levchenko, Tatyana S.Torchilin, Vladimir P.TAT peptideBovine herpes virus 1 glycoprotein DDendritic cell transfectionGreen fluorescent proteinhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Dendritic cells (DC) are antigen presenting cells (APC) uniquely capable of priming naïve T cells and cross-presenting antigens and determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by an enhanced expression of the enhanced green fluorescent protein (EGFP) and bovine herpes virus type 1 (BoHV-1) glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L as indicated by the secretion of interleukin 6 (IL-6).Fil: Pappalardo, Juan Sebastian. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Hartner, William C.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosFil: Levchenko, Tatyana S.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosFil: Torchilin, Vladimir P.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosDove Press2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33327Olivera, Valeria; Di Giacomo, Sebastián; Pappalardo, Juan Sebastian; Levchenko, Tatyana S.; Quattrocchi, Valeria; Hartner, William C.; et al.; In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes; Dove Press; International Journal of Nanomedicine; 9; 1; 2-2014; 963-9731176-91141178-2013CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928453/info:eu-repo/semantics/altIdentifier/doi/10.2147/IJN.S53432info:eu-repo/semantics/altIdentifier/url/https://www.dovepress.com/in-vitro-transfection-of-bone-marrow-derived-dendritic-cells-with-tatp-peer-reviewed-article-IJNinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T09:11:25Zoai:ri.conicet.gov.ar:11336/33327instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 09:11:25.97CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
spellingShingle In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
Pappalardo, Juan Sebastian
TAT peptide
Bovine herpes virus 1 glycoprotein D
Dendritic cell transfection
Green fluorescent protein
title_short In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_full In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_fullStr In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_full_unstemmed In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
title_sort In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
dc.creator.none.fl_str_mv Pappalardo, Juan Sebastian
Langellotti, Cecilia Ana
Di Giacomo, Sebastián
Olivera, Valeria
Quattrocchi, Valeria
Zamorano, Patricia Ines
Hartner, William C.
Levchenko, Tatyana S.
Torchilin, Vladimir P.
author Pappalardo, Juan Sebastian
author_facet Pappalardo, Juan Sebastian
Langellotti, Cecilia Ana
Di Giacomo, Sebastián
Olivera, Valeria
Quattrocchi, Valeria
Zamorano, Patricia Ines
Hartner, William C.
Levchenko, Tatyana S.
Torchilin, Vladimir P.
author_role author
author2 Langellotti, Cecilia Ana
Di Giacomo, Sebastián
Olivera, Valeria
Quattrocchi, Valeria
Zamorano, Patricia Ines
Hartner, William C.
Levchenko, Tatyana S.
Torchilin, Vladimir P.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TAT peptide
Bovine herpes virus 1 glycoprotein D
Dendritic cell transfection
Green fluorescent protein
topic TAT peptide
Bovine herpes virus 1 glycoprotein D
Dendritic cell transfection
Green fluorescent protein
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Dendritic cells (DC) are antigen presenting cells (APC) uniquely capable of priming naïve T cells and cross-presenting antigens and determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by an enhanced expression of the enhanced green fluorescent protein (EGFP) and bovine herpes virus type 1 (BoHV-1) glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L as indicated by the secretion of interleukin 6 (IL-6).
Fil: Pappalardo, Juan Sebastian. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Hartner, William C.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Levchenko, Tatyana S.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Torchilin, Vladimir P.. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
description Dendritic cells (DC) are antigen presenting cells (APC) uniquely capable of priming naïve T cells and cross-presenting antigens and determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by an enhanced expression of the enhanced green fluorescent protein (EGFP) and bovine herpes virus type 1 (BoHV-1) glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L as indicated by the secretion of interleukin 6 (IL-6).
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33327
Olivera, Valeria; Di Giacomo, Sebastián; Pappalardo, Juan Sebastian; Levchenko, Tatyana S.; Quattrocchi, Valeria; Hartner, William C.; et al.; In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes; Dove Press; International Journal of Nanomedicine; 9; 1; 2-2014; 963-973
1176-9114
1178-2013
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33327
identifier_str_mv Olivera, Valeria; Di Giacomo, Sebastián; Pappalardo, Juan Sebastian; Levchenko, Tatyana S.; Quattrocchi, Valeria; Hartner, William C.; et al.; In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes; Dove Press; International Journal of Nanomedicine; 9; 1; 2-2014; 963-973
1176-9114
1178-2013
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928453/
info:eu-repo/semantics/altIdentifier/doi/10.2147/IJN.S53432
info:eu-repo/semantics/altIdentifier/url/https://www.dovepress.com/in-vitro-transfection-of-bone-marrow-derived-dendritic-cells-with-tatp-peer-reviewed-article-IJN
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Dove Press
publisher.none.fl_str_mv Dove Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.011256

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