XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode

Autores
Scodeller, Pablo David; Williams, Federico José; Calvo, Ernesto Julio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High potential purified Trametes trogii laccase has been deposited in mono- and multilayer thin films on gold surfaces by layer-by-layer electrostatic adsorption self-assembly. The osmium bipyridil redox relay sites on polycation poly(allylamine) backbone efficiently work as a molecular “wire” in oxygen cathodes for biofuel cells. X-ray photoelectron spectroscopy of Cu 2p3/2 and Os 4f signals provided chemical information on the enzyme and redox mediator surface concentrations after different adsorption steps. The electrical charge involved in oxidation–reduction cycles of the osmium sites, the ellipsometric enzyme film thickness, and the mass uptake from quartz crystal microbalance experiments, correlate with the XPS surface concentration, which provides unique evidence on the chemical identity of the composition in the topmost layers. XPS is shown to be an important analytical tool to investigate stratified copper and osmium distribution in LbL thin films relevant to biosensors and biofuel cells.
Fil: Scodeller, Pablo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Williams, Federico José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Calvo, Ernesto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
Xps
Laccase
Layer by Layer
Osmium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31725
Acceder
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network_name_str CONICET Digital (CONICET)
spelling XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme ElectrodeScodeller, Pablo DavidWilliams, Federico JoséCalvo, Ernesto JulioXpsLaccaseLayer by LayerOsmiumhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1High potential purified Trametes trogii laccase has been deposited in mono- and multilayer thin films on gold surfaces by layer-by-layer electrostatic adsorption self-assembly. The osmium bipyridil redox relay sites on polycation poly(allylamine) backbone efficiently work as a molecular “wire” in oxygen cathodes for biofuel cells. X-ray photoelectron spectroscopy of Cu 2p3/2 and Os 4f signals provided chemical information on the enzyme and redox mediator surface concentrations after different adsorption steps. The electrical charge involved in oxidation–reduction cycles of the osmium sites, the ellipsometric enzyme film thickness, and the mass uptake from quartz crystal microbalance experiments, correlate with the XPS surface concentration, which provides unique evidence on the chemical identity of the composition in the topmost layers. XPS is shown to be an important analytical tool to investigate stratified copper and osmium distribution in LbL thin films relevant to biosensors and biofuel cells.Fil: Scodeller, Pablo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Williams, Federico José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Calvo, Ernesto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaAmerican Chemical Society2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31725Calvo, Ernesto Julio; Williams, Federico José; Scodeller, Pablo David; XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode; American Chemical Society; Analytical Chemistry; 86; 24; 11-2014; 12180-121840003-2700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/ac503147cinfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/ac503147cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:26Zoai:ri.conicet.gov.ar:11336/31725instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:26.746CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
title XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
spellingShingle XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
Scodeller, Pablo David
Xps
Laccase
Layer by Layer
Osmium
title_short XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
title_full XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
title_fullStr XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
title_full_unstemmed XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
title_sort XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode
dc.creator.none.fl_str_mv Scodeller, Pablo David
Williams, Federico José
Calvo, Ernesto Julio
author Scodeller, Pablo David
author_facet Scodeller, Pablo David
Williams, Federico José
Calvo, Ernesto Julio
author_role author
author2 Williams, Federico José
Calvo, Ernesto Julio
author2_role author
author
dc.subject.none.fl_str_mv Xps
Laccase
Layer by Layer
Osmium
topic Xps
Laccase
Layer by Layer
Osmium
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High potential purified Trametes trogii laccase has been deposited in mono- and multilayer thin films on gold surfaces by layer-by-layer electrostatic adsorption self-assembly. The osmium bipyridil redox relay sites on polycation poly(allylamine) backbone efficiently work as a molecular “wire” in oxygen cathodes for biofuel cells. X-ray photoelectron spectroscopy of Cu 2p3/2 and Os 4f signals provided chemical information on the enzyme and redox mediator surface concentrations after different adsorption steps. The electrical charge involved in oxidation–reduction cycles of the osmium sites, the ellipsometric enzyme film thickness, and the mass uptake from quartz crystal microbalance experiments, correlate with the XPS surface concentration, which provides unique evidence on the chemical identity of the composition in the topmost layers. XPS is shown to be an important analytical tool to investigate stratified copper and osmium distribution in LbL thin films relevant to biosensors and biofuel cells.
Fil: Scodeller, Pablo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Williams, Federico José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Calvo, Ernesto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description High potential purified Trametes trogii laccase has been deposited in mono- and multilayer thin films on gold surfaces by layer-by-layer electrostatic adsorption self-assembly. The osmium bipyridil redox relay sites on polycation poly(allylamine) backbone efficiently work as a molecular “wire” in oxygen cathodes for biofuel cells. X-ray photoelectron spectroscopy of Cu 2p3/2 and Os 4f signals provided chemical information on the enzyme and redox mediator surface concentrations after different adsorption steps. The electrical charge involved in oxidation–reduction cycles of the osmium sites, the ellipsometric enzyme film thickness, and the mass uptake from quartz crystal microbalance experiments, correlate with the XPS surface concentration, which provides unique evidence on the chemical identity of the composition in the topmost layers. XPS is shown to be an important analytical tool to investigate stratified copper and osmium distribution in LbL thin films relevant to biosensors and biofuel cells.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31725
Calvo, Ernesto Julio; Williams, Federico José; Scodeller, Pablo David; XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode; American Chemical Society; Analytical Chemistry; 86; 24; 11-2014; 12180-12184
0003-2700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31725
identifier_str_mv Calvo, Ernesto Julio; Williams, Federico José; Scodeller, Pablo David; XPS Analysis of Enzyme and Mediator at the Surface of a Layer-by-Layer Self-Assembled Wired Enzyme Electrode; American Chemical Society; Analytical Chemistry; 86; 24; 11-2014; 12180-12184
0003-2700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/ac503147c
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/ac503147c
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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