Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression

Autores
Nagymihály, Marianna; Veluchamy, Alaguraj; Györgypál, Zoltán; Ariel, Federico Damian; Jégu, Teddy; Benhamed, Moussa; Szücs, Attila; Kereszt, Attila; Mergaert, Peter; Kondorosi, Éva
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodulespecific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes´ active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplicationdependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.
Fil: Nagymihály, Marianna. Université Paris Sud; Francia. Hungarian Academy of Sciences; Hungría
Fil: Veluchamy, Alaguraj. King Abdullah University of Science and Technology; Arabia Saudita
Fil: Györgypál, Zoltán. Hungarian Academy of Sciences; Hungría
Fil: Ariel, Federico Damian. Instituto de Agrobiotecnología del Litoral (conicet-unl); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Jégu, Teddy. Université Paris Sud; Francia
Fil: Benhamed, Moussa. Université Paris Sud; Francia
Fil: Szücs, Attila. Hungarian Academy of Sciences; Hungría
Fil: Kereszt, Attila. Biological Research Center At Hungarian Academy Of Sciences;
Fil: Mergaert, Peter. Université Paris Sud; Francia
Fil: Kondorosi, Éva. Université Paris Sud; Francia. Hungarian Academy of Sciences; Hungría
Materia
Chromatin Structure
Dna Methylation
Medicago Truncatula
Nodule-Specific Cysteine-Rich Peptides
Symbiosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46428
Acceder
id CONICETDig_3061a5cd45ac4633572d442f97815b96
oai_identifier_str oai:ri.conicet.gov.ar:11336/46428
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expressionNagymihály, MariannaVeluchamy, AlagurajGyörgypál, ZoltánAriel, Federico DamianJégu, TeddyBenhamed, MoussaSzücs, AttilaKereszt, AttilaMergaert, PeterKondorosi, ÉvaChromatin StructureDna MethylationMedicago TruncatulaNodule-Specific Cysteine-Rich PeptidesSymbiosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodulespecific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes´ active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplicationdependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.Fil: Nagymihály, Marianna. Université Paris Sud; Francia. Hungarian Academy of Sciences; HungríaFil: Veluchamy, Alaguraj. King Abdullah University of Science and Technology; Arabia SauditaFil: Györgypál, Zoltán. Hungarian Academy of Sciences; HungríaFil: Ariel, Federico Damian. Instituto de Agrobiotecnología del Litoral (conicet-unl); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Jégu, Teddy. Université Paris Sud; FranciaFil: Benhamed, Moussa. Université Paris Sud; FranciaFil: Szücs, Attila. Hungarian Academy of Sciences; HungríaFil: Kereszt, Attila. Biological Research Center At Hungarian Academy Of Sciences;Fil: Mergaert, Peter. Université Paris Sud; FranciaFil: Kondorosi, Éva. Université Paris Sud; Francia. Hungarian Academy of Sciences; HungríaNational Academy of Sciences2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46428Nagymihály, Marianna; Veluchamy, Alaguraj; Györgypál, Zoltán; Ariel, Federico Damian; Jégu, Teddy; et al.; Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 114; 17; 4-2017; 4543-45480027-8424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1704211114info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:02:03Zoai:ri.conicet.gov.ar:11336/46428instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:02:03.689CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
title Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
spellingShingle Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
Nagymihály, Marianna
Chromatin Structure
Dna Methylation
Medicago Truncatula
Nodule-Specific Cysteine-Rich Peptides
Symbiosis
title_short Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
title_full Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
title_fullStr Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
title_full_unstemmed Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
title_sort Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
dc.creator.none.fl_str_mv Nagymihály, Marianna
Veluchamy, Alaguraj
Györgypál, Zoltán
Ariel, Federico Damian
Jégu, Teddy
Benhamed, Moussa
Szücs, Attila
Kereszt, Attila
Mergaert, Peter
Kondorosi, Éva
author Nagymihály, Marianna
author_facet Nagymihály, Marianna
Veluchamy, Alaguraj
Györgypál, Zoltán
Ariel, Federico Damian
Jégu, Teddy
Benhamed, Moussa
Szücs, Attila
Kereszt, Attila
Mergaert, Peter
Kondorosi, Éva
author_role author
author2 Veluchamy, Alaguraj
Györgypál, Zoltán
Ariel, Federico Damian
Jégu, Teddy
Benhamed, Moussa
Szücs, Attila
Kereszt, Attila
Mergaert, Peter
Kondorosi, Éva
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chromatin Structure
Dna Methylation
Medicago Truncatula
Nodule-Specific Cysteine-Rich Peptides
Symbiosis
topic Chromatin Structure
Dna Methylation
Medicago Truncatula
Nodule-Specific Cysteine-Rich Peptides
Symbiosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodulespecific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes´ active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplicationdependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.
Fil: Nagymihály, Marianna. Université Paris Sud; Francia. Hungarian Academy of Sciences; Hungría
Fil: Veluchamy, Alaguraj. King Abdullah University of Science and Technology; Arabia Saudita
Fil: Györgypál, Zoltán. Hungarian Academy of Sciences; Hungría
Fil: Ariel, Federico Damian. Instituto de Agrobiotecnología del Litoral (conicet-unl); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Jégu, Teddy. Université Paris Sud; Francia
Fil: Benhamed, Moussa. Université Paris Sud; Francia
Fil: Szücs, Attila. Hungarian Academy of Sciences; Hungría
Fil: Kereszt, Attila. Biological Research Center At Hungarian Academy Of Sciences;
Fil: Mergaert, Peter. Université Paris Sud; Francia
Fil: Kondorosi, Éva. Université Paris Sud; Francia. Hungarian Academy of Sciences; Hungría
description The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodulespecific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes´ active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplicationdependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46428
Nagymihály, Marianna; Veluchamy, Alaguraj; Györgypál, Zoltán; Ariel, Federico Damian; Jégu, Teddy; et al.; Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 114; 17; 4-2017; 4543-4548
0027-8424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46428
identifier_str_mv Nagymihály, Marianna; Veluchamy, Alaguraj; Györgypál, Zoltán; Ariel, Federico Damian; Jégu, Teddy; et al.; Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 114; 17; 4-2017; 4543-4548
0027-8424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1704211114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782355520356352
score 12.982451

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