Two direct targets of cytokinin signaling control symbiotic nodulation

Autores
Ariel, Federico Damian; Brault Hernandez, Marianne; Laffont, Carole; Huault, Emeline; Brault, Mathias; Plet, Julie; Moison, Michael; Blanchet, Sandrine; Ichanté, Jean Laurent; Chabaud, Mireille; Carrere, Sébastien; Crespi, Martin; Chan, Raquel Lia; Frugier, Florian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytokinin regulates many aspects of plant development, and in legume crops, this  phytohormone is necessary and sufficient for symbiotic nodule organogenesis, allowing them to fix atmospheric nitrogen. To identify direct links between cytokinins and nodule organogenesis, we determined a consensus sequence bound in vitro by a transcription factor (TF) acting in cytokinin signaling, the nodule-enhanced Medicago truncatula Mt RR1 response regulator (RR). Among genes rapidly regulated by cytokinins and containing this so-called RR binding site (RRBS) in their promoters, we found the nodulationrelatedType-A RR Mt RR4 and the Nodulation Signaling Pathway 2 (NSP2) TF. Site-directed mutagenesis revealed that RRBS cis-elements in the RR4 and NSP2 promoters are essential for expression during nodule development and for cytokinin induction. Furthermore, a microRNA targeting NSP2 (miR171 h) is also rapidly induced by cytokinins and then shows an expression pattern anticorrelated with NSP2. Other primary targets regulated by cytokinins depending on the Cytokinin Response1 (CRE1) receptor were a cytokinin oxidase/dehydrogenase (CKX1) and a basic Helix-Loop-Helix TF (bHLH476). RNA interference constructs as well as insertion of a Tnt1 retrotransposon in the bHLH gene led to reduced nodulation. Hence, we identified two TFs, NSP2 and bHLH476, as direct cytokinin targets acting at the convergence of phytohormonal and symbiotic cues.
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Centre National de la Recherche Scientifique; Francia
Fil: Brault Hernandez, Marianne. Centre National de la Recherche Scientifique; Francia
Fil: Laffont, Carole. Centre National de la Recherche Scientifique; Francia
Fil: Huault, Emeline. Centre National de la Recherche Scientifique; Francia
Fil: Brault, Mathias. Centre National de la Recherche Scientifique; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Plet, Julie. Centre National de la Recherche Scientifique; Francia
Fil: Moison, Michael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Centre National de la Recherche Scientifique; Francia
Fil: Blanchet, Sandrine. Centre National de la Recherche Scientifique; Francia
Fil: Ichanté, Jean Laurent. Centre National de la Recherche Scientifique; Francia
Fil: Chabaud, Mireille. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Carrere, Sébastien. Centre National de la Recherche Scientifique; Francia
Fil: Crespi, Martin. Centre National de la Recherche Scientifique; Francia
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Frugier, Florian. Centre National de la Recherche Scientifique; Francia
Materia
Factores de transcripción
MiRNA
Medicago truncatula
Citoquinas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272139
Acceder
id CONICETDig_27f888328ca21fa995963735400dfd08
oai_identifier_str oai:ri.conicet.gov.ar:11336/272139
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Two direct targets of cytokinin signaling control symbiotic nodulationAriel, Federico DamianBrault Hernandez, MarianneLaffont, CaroleHuault, EmelineBrault, MathiasPlet, JulieMoison, MichaelBlanchet, SandrineIchanté, Jean LaurentChabaud, MireilleCarrere, SébastienCrespi, MartinChan, Raquel LiaFrugier, FlorianFactores de transcripciónMiRNAMedicago truncatulaCitoquinashttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cytokinin regulates many aspects of plant development, and in legume crops, this  phytohormone is necessary and sufficient for symbiotic nodule organogenesis, allowing them to fix atmospheric nitrogen. To identify direct links between cytokinins and nodule organogenesis, we determined a consensus sequence bound in vitro by a transcription factor (TF) acting in cytokinin signaling, the nodule-enhanced Medicago truncatula Mt RR1 response regulator (RR). Among genes rapidly regulated by cytokinins and containing this so-called RR binding site (RRBS) in their promoters, we found the nodulationrelatedType-A RR Mt RR4 and the Nodulation Signaling Pathway 2 (NSP2) TF. Site-directed mutagenesis revealed that RRBS cis-elements in the RR4 and NSP2 promoters are essential for expression during nodule development and for cytokinin induction. Furthermore, a microRNA targeting NSP2 (miR171 h) is also rapidly induced by cytokinins and then shows an expression pattern anticorrelated with NSP2. Other primary targets regulated by cytokinins depending on the Cytokinin Response1 (CRE1) receptor were a cytokinin oxidase/dehydrogenase (CKX1) and a basic Helix-Loop-Helix TF (bHLH476). RNA interference constructs as well as insertion of a Tnt1 retrotransposon in the bHLH gene led to reduced nodulation. Hence, we identified two TFs, NSP2 and bHLH476, as direct cytokinin targets acting at the convergence of phytohormonal and symbiotic cues.Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Centre National de la Recherche Scientifique; FranciaFil: Brault Hernandez, Marianne. Centre National de la Recherche Scientifique; FranciaFil: Laffont, Carole. Centre National de la Recherche Scientifique; FranciaFil: Huault, Emeline. Centre National de la Recherche Scientifique; FranciaFil: Brault, Mathias. Centre National de la Recherche Scientifique; Francia. Université Paris Diderot - Paris 7; FranciaFil: Plet, Julie. Centre National de la Recherche Scientifique; FranciaFil: Moison, Michael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Centre National de la Recherche Scientifique; FranciaFil: Blanchet, Sandrine. Centre National de la Recherche Scientifique; FranciaFil: Ichanté, Jean Laurent. Centre National de la Recherche Scientifique; FranciaFil: Chabaud, Mireille. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; FranciaFil: Carrere, Sébastien. Centre National de la Recherche Scientifique; FranciaFil: Crespi, Martin. Centre National de la Recherche Scientifique; FranciaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Frugier, Florian. Centre National de la Recherche Scientifique; FranciaAmerican Society of Plant Biologist2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272139Ariel, Federico Damian; Brault Hernandez, Marianne; Laffont, Carole; Huault, Emeline; Brault, Mathias; et al.; Two direct targets of cytokinin signaling control symbiotic nodulation; American Society of Plant Biologist; Plant Cell; 24; 9; 9-2012; 3838-38521040-4651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/24/9/3838/6100604info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.112.103267info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:11:30Zoai:ri.conicet.gov.ar:11336/272139instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:11:30.44CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Two direct targets of cytokinin signaling control symbiotic nodulation
title Two direct targets of cytokinin signaling control symbiotic nodulation
spellingShingle Two direct targets of cytokinin signaling control symbiotic nodulation
Ariel, Federico Damian
Factores de transcripción
MiRNA
Medicago truncatula
Citoquinas
title_short Two direct targets of cytokinin signaling control symbiotic nodulation
title_full Two direct targets of cytokinin signaling control symbiotic nodulation
title_fullStr Two direct targets of cytokinin signaling control symbiotic nodulation
title_full_unstemmed Two direct targets of cytokinin signaling control symbiotic nodulation
title_sort Two direct targets of cytokinin signaling control symbiotic nodulation
dc.creator.none.fl_str_mv Ariel, Federico Damian
Brault Hernandez, Marianne
Laffont, Carole
Huault, Emeline
Brault, Mathias
Plet, Julie
Moison, Michael
Blanchet, Sandrine
Ichanté, Jean Laurent
Chabaud, Mireille
Carrere, Sébastien
Crespi, Martin
Chan, Raquel Lia
Frugier, Florian
author Ariel, Federico Damian
author_facet Ariel, Federico Damian
Brault Hernandez, Marianne
Laffont, Carole
Huault, Emeline
Brault, Mathias
Plet, Julie
Moison, Michael
Blanchet, Sandrine
Ichanté, Jean Laurent
Chabaud, Mireille
Carrere, Sébastien
Crespi, Martin
Chan, Raquel Lia
Frugier, Florian
author_role author
author2 Brault Hernandez, Marianne
Laffont, Carole
Huault, Emeline
Brault, Mathias
Plet, Julie
Moison, Michael
Blanchet, Sandrine
Ichanté, Jean Laurent
Chabaud, Mireille
Carrere, Sébastien
Crespi, Martin
Chan, Raquel Lia
Frugier, Florian
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Factores de transcripción
MiRNA
Medicago truncatula
Citoquinas
topic Factores de transcripción
MiRNA
Medicago truncatula
Citoquinas
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cytokinin regulates many aspects of plant development, and in legume crops, this  phytohormone is necessary and sufficient for symbiotic nodule organogenesis, allowing them to fix atmospheric nitrogen. To identify direct links between cytokinins and nodule organogenesis, we determined a consensus sequence bound in vitro by a transcription factor (TF) acting in cytokinin signaling, the nodule-enhanced Medicago truncatula Mt RR1 response regulator (RR). Among genes rapidly regulated by cytokinins and containing this so-called RR binding site (RRBS) in their promoters, we found the nodulationrelatedType-A RR Mt RR4 and the Nodulation Signaling Pathway 2 (NSP2) TF. Site-directed mutagenesis revealed that RRBS cis-elements in the RR4 and NSP2 promoters are essential for expression during nodule development and for cytokinin induction. Furthermore, a microRNA targeting NSP2 (miR171 h) is also rapidly induced by cytokinins and then shows an expression pattern anticorrelated with NSP2. Other primary targets regulated by cytokinins depending on the Cytokinin Response1 (CRE1) receptor were a cytokinin oxidase/dehydrogenase (CKX1) and a basic Helix-Loop-Helix TF (bHLH476). RNA interference constructs as well as insertion of a Tnt1 retrotransposon in the bHLH gene led to reduced nodulation. Hence, we identified two TFs, NSP2 and bHLH476, as direct cytokinin targets acting at the convergence of phytohormonal and symbiotic cues.
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Centre National de la Recherche Scientifique; Francia
Fil: Brault Hernandez, Marianne. Centre National de la Recherche Scientifique; Francia
Fil: Laffont, Carole. Centre National de la Recherche Scientifique; Francia
Fil: Huault, Emeline. Centre National de la Recherche Scientifique; Francia
Fil: Brault, Mathias. Centre National de la Recherche Scientifique; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Plet, Julie. Centre National de la Recherche Scientifique; Francia
Fil: Moison, Michael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Centre National de la Recherche Scientifique; Francia
Fil: Blanchet, Sandrine. Centre National de la Recherche Scientifique; Francia
Fil: Ichanté, Jean Laurent. Centre National de la Recherche Scientifique; Francia
Fil: Chabaud, Mireille. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Carrere, Sébastien. Centre National de la Recherche Scientifique; Francia
Fil: Crespi, Martin. Centre National de la Recherche Scientifique; Francia
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Frugier, Florian. Centre National de la Recherche Scientifique; Francia
description Cytokinin regulates many aspects of plant development, and in legume crops, this  phytohormone is necessary and sufficient for symbiotic nodule organogenesis, allowing them to fix atmospheric nitrogen. To identify direct links between cytokinins and nodule organogenesis, we determined a consensus sequence bound in vitro by a transcription factor (TF) acting in cytokinin signaling, the nodule-enhanced Medicago truncatula Mt RR1 response regulator (RR). Among genes rapidly regulated by cytokinins and containing this so-called RR binding site (RRBS) in their promoters, we found the nodulationrelatedType-A RR Mt RR4 and the Nodulation Signaling Pathway 2 (NSP2) TF. Site-directed mutagenesis revealed that RRBS cis-elements in the RR4 and NSP2 promoters are essential for expression during nodule development and for cytokinin induction. Furthermore, a microRNA targeting NSP2 (miR171 h) is also rapidly induced by cytokinins and then shows an expression pattern anticorrelated with NSP2. Other primary targets regulated by cytokinins depending on the Cytokinin Response1 (CRE1) receptor were a cytokinin oxidase/dehydrogenase (CKX1) and a basic Helix-Loop-Helix TF (bHLH476). RNA interference constructs as well as insertion of a Tnt1 retrotransposon in the bHLH gene led to reduced nodulation. Hence, we identified two TFs, NSP2 and bHLH476, as direct cytokinin targets acting at the convergence of phytohormonal and symbiotic cues.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272139
Ariel, Federico Damian; Brault Hernandez, Marianne; Laffont, Carole; Huault, Emeline; Brault, Mathias; et al.; Two direct targets of cytokinin signaling control symbiotic nodulation; American Society of Plant Biologist; Plant Cell; 24; 9; 9-2012; 3838-3852
1040-4651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272139
identifier_str_mv Ariel, Federico Damian; Brault Hernandez, Marianne; Laffont, Carole; Huault, Emeline; Brault, Mathias; et al.; Two direct targets of cytokinin signaling control symbiotic nodulation; American Society of Plant Biologist; Plant Cell; 24; 9; 9-2012; 3838-3852
1040-4651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/24/9/3838/6100604
info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.112.103267
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781493369634816
score 12.982451

Publicaciones similares