Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes
- Autores
- Ferreira, Maria Laura; Casabuono, Adriana Cristina; Stacchiotti, Sabrina Tamara; Couto, Alicia Susana; Ramírez, Silvana Andrea María; Vullo, Diana Lia
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l-1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 µmoles Cd(II) binding sites g LPS-1). This information is a valuable tool for the application of EPS in industrial wastewater treatment.
Fil: Ferreira, Maria Laura. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Casabuono, Adriana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Stacchiotti, Sabrina Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Ramírez, Silvana Andrea María. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Vullo, Diana Lia. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Extracellular Polymeric Substances (Eps)
Bacterial Lipopolysaccharide (Lps)
Pseudomonas Veronii 2e
Cadmium Retention
Biotreatment - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/40680
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Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastesFerreira, Maria LauraCasabuono, Adriana CristinaStacchiotti, Sabrina TamaraCouto, Alicia SusanaRamírez, Silvana Andrea MaríaVullo, Diana LiaExtracellular Polymeric Substances (Eps)Bacterial Lipopolysaccharide (Lps)Pseudomonas Veronii 2eCadmium RetentionBiotreatmenthttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l-1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 µmoles Cd(II) binding sites g LPS-1). This information is a valuable tool for the application of EPS in industrial wastewater treatment.Fil: Ferreira, Maria Laura. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Casabuono, Adriana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Stacchiotti, Sabrina Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Ramírez, Silvana Andrea María. Universidad Nacional de General Sarmiento. Instituto de Ciencias; ArgentinaFil: Vullo, Diana Lia. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40680Ferreira, Maria Laura; Casabuono, Adriana Cristina; Stacchiotti, Sabrina Tamara; Couto, Alicia Susana; Ramírez, Silvana Andrea María; et al.; Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes; Elsevier; International Biodeterioration and Biodegradation; 119; 3-2017; 605-6130964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2016.10.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830516305133info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:20Zoai:ri.conicet.gov.ar:11336/40680instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:21.004CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
title |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
spellingShingle |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes Ferreira, Maria Laura Extracellular Polymeric Substances (Eps) Bacterial Lipopolysaccharide (Lps) Pseudomonas Veronii 2e Cadmium Retention Biotreatment |
title_short |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
title_full |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
title_fullStr |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
title_full_unstemmed |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
title_sort |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
dc.creator.none.fl_str_mv |
Ferreira, Maria Laura Casabuono, Adriana Cristina Stacchiotti, Sabrina Tamara Couto, Alicia Susana Ramírez, Silvana Andrea María Vullo, Diana Lia |
author |
Ferreira, Maria Laura |
author_facet |
Ferreira, Maria Laura Casabuono, Adriana Cristina Stacchiotti, Sabrina Tamara Couto, Alicia Susana Ramírez, Silvana Andrea María Vullo, Diana Lia |
author_role |
author |
author2 |
Casabuono, Adriana Cristina Stacchiotti, Sabrina Tamara Couto, Alicia Susana Ramírez, Silvana Andrea María Vullo, Diana Lia |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Extracellular Polymeric Substances (Eps) Bacterial Lipopolysaccharide (Lps) Pseudomonas Veronii 2e Cadmium Retention Biotreatment |
topic |
Extracellular Polymeric Substances (Eps) Bacterial Lipopolysaccharide (Lps) Pseudomonas Veronii 2e Cadmium Retention Biotreatment |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l-1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 µmoles Cd(II) binding sites g LPS-1). This information is a valuable tool for the application of EPS in industrial wastewater treatment. Fil: Ferreira, Maria Laura. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Casabuono, Adriana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Stacchiotti, Sabrina Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Ramírez, Silvana Andrea María. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina Fil: Vullo, Diana Lia. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l-1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 µmoles Cd(II) binding sites g LPS-1). This information is a valuable tool for the application of EPS in industrial wastewater treatment. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/40680 Ferreira, Maria Laura; Casabuono, Adriana Cristina; Stacchiotti, Sabrina Tamara; Couto, Alicia Susana; Ramírez, Silvana Andrea María; et al.; Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes; Elsevier; International Biodeterioration and Biodegradation; 119; 3-2017; 605-613 0964-8305 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/40680 |
identifier_str_mv |
Ferreira, Maria Laura; Casabuono, Adriana Cristina; Stacchiotti, Sabrina Tamara; Couto, Alicia Susana; Ramírez, Silvana Andrea María; et al.; Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes; Elsevier; International Biodeterioration and Biodegradation; 119; 3-2017; 605-613 0964-8305 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2016.10.013 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830516305133 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613578697474048 |
score |
13.070432 |