Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing

Autores
Morcillo, N.; Imperiale, Belén Rocío; Di Giulio, B.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
SETTING: Dr Cetrángolo Hospital, Buenos Aires Province, Argentina. OBJECTIVE: Evaluation of the BACTEC? Mycobacteria Growth Indicator Tube (MGIT)? 960 system and the colorimetric-based method (CMM) for fi rst- and second line drug susceptibility testing (FL-DST, SL-DST) against Mycobacterium tuberculosis. DESIGN: FL-DST was studied using SIRE MGIT 960. Minimal inhibitory concentrations (MICs) for isoniazid (INH), streptomycin, rifampicin (RMP), ethambutol (EMB) and levofl oxacin (LVX) were also determined by CMM using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT). MICs for amikacin (AMK), kanamycin (KM), capreomycin (CPM), ethionamide (ETH), cycloserine, ofl oxacin (OFX), linezolide (LZ) and moxifl oxacin (MFX) were determined on 94 multidrug resistant M. tuberculosis isolates by MGIT 960 and CMM. Statistical methods were applied to define drug susceptible and drug-resistant isolates on the basis of the comparison between results obtained by gold standards. RESULTS: A total of 1626 clinical isolates were studied. Critical drug concentrations could be defi ned in less than 10 days for both CMM and MGIT 960. CMM was cheaper but more laborious than MGIT 960. The highest performances of both methods were achieved for AMK, RMP, OFX, LZ and MFX, followed by INH, ETH, KM, CPM and LVX (tested only by CMM). CONCLUSIONS: Both methods could be implemented as rapid diagnostic tools to detect drug-resistant isolates in clinical practice.
Fil: Morcillo, N.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giulio, B.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Materia
TUBERCULOSIS
DRUG- SUSCEPTIBILITY
MGIT960
COLORIMETRIC METHODS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190897
Acceder
id CONICETDig_2fea0f8eeb496e6075e8b432edeac5d4
oai_identifier_str oai:ri.conicet.gov.ar:11336/190897
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testingMorcillo, N.Imperiale, Belén RocíoDi Giulio, B.TUBERCULOSISDRUG- SUSCEPTIBILITYMGIT960COLORIMETRIC METHODShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1SETTING: Dr Cetrángolo Hospital, Buenos Aires Province, Argentina. OBJECTIVE: Evaluation of the BACTEC? Mycobacteria Growth Indicator Tube (MGIT)? 960 system and the colorimetric-based method (CMM) for fi rst- and second line drug susceptibility testing (FL-DST, SL-DST) against Mycobacterium tuberculosis. DESIGN: FL-DST was studied using SIRE MGIT 960. Minimal inhibitory concentrations (MICs) for isoniazid (INH), streptomycin, rifampicin (RMP), ethambutol (EMB) and levofl oxacin (LVX) were also determined by CMM using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT). MICs for amikacin (AMK), kanamycin (KM), capreomycin (CPM), ethionamide (ETH), cycloserine, ofl oxacin (OFX), linezolide (LZ) and moxifl oxacin (MFX) were determined on 94 multidrug resistant M. tuberculosis isolates by MGIT 960 and CMM. Statistical methods were applied to define drug susceptible and drug-resistant isolates on the basis of the comparison between results obtained by gold standards. RESULTS: A total of 1626 clinical isolates were studied. Critical drug concentrations could be defi ned in less than 10 days for both CMM and MGIT 960. CMM was cheaper but more laborious than MGIT 960. The highest performances of both methods were achieved for AMK, RMP, OFX, LZ and MFX, followed by INH, ETH, KM, CPM and LVX (tested only by CMM). CONCLUSIONS: Both methods could be implemented as rapid diagnostic tools to detect drug-resistant isolates in clinical practice.Fil: Morcillo, N.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Giulio, B.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaInternational Union Against Tuberculosis and Lung Disease2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190897Morcillo, N.; Imperiale, Belén Rocío; Di Giulio, B.; Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 14; 9; 9-2010; 1169-11751027-3719CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2010/00000014/00000009/art00017;jsessionid=6m97djap025ii.x-ic-live-02info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:57Zoai:ri.conicet.gov.ar:11336/190897instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:57.471CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
title Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
spellingShingle Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
Morcillo, N.
TUBERCULOSIS
DRUG- SUSCEPTIBILITY
MGIT960
COLORIMETRIC METHODS
title_short Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
title_full Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
title_fullStr Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
title_full_unstemmed Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
title_sort Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
dc.creator.none.fl_str_mv Morcillo, N.
Imperiale, Belén Rocío
Di Giulio, B.
author Morcillo, N.
author_facet Morcillo, N.
Imperiale, Belén Rocío
Di Giulio, B.
author_role author
author2 Imperiale, Belén Rocío
Di Giulio, B.
author2_role author
author
dc.subject.none.fl_str_mv TUBERCULOSIS
DRUG- SUSCEPTIBILITY
MGIT960
COLORIMETRIC METHODS
topic TUBERCULOSIS
DRUG- SUSCEPTIBILITY
MGIT960
COLORIMETRIC METHODS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv SETTING: Dr Cetrángolo Hospital, Buenos Aires Province, Argentina. OBJECTIVE: Evaluation of the BACTEC? Mycobacteria Growth Indicator Tube (MGIT)? 960 system and the colorimetric-based method (CMM) for fi rst- and second line drug susceptibility testing (FL-DST, SL-DST) against Mycobacterium tuberculosis. DESIGN: FL-DST was studied using SIRE MGIT 960. Minimal inhibitory concentrations (MICs) for isoniazid (INH), streptomycin, rifampicin (RMP), ethambutol (EMB) and levofl oxacin (LVX) were also determined by CMM using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT). MICs for amikacin (AMK), kanamycin (KM), capreomycin (CPM), ethionamide (ETH), cycloserine, ofl oxacin (OFX), linezolide (LZ) and moxifl oxacin (MFX) were determined on 94 multidrug resistant M. tuberculosis isolates by MGIT 960 and CMM. Statistical methods were applied to define drug susceptible and drug-resistant isolates on the basis of the comparison between results obtained by gold standards. RESULTS: A total of 1626 clinical isolates were studied. Critical drug concentrations could be defi ned in less than 10 days for both CMM and MGIT 960. CMM was cheaper but more laborious than MGIT 960. The highest performances of both methods were achieved for AMK, RMP, OFX, LZ and MFX, followed by INH, ETH, KM, CPM and LVX (tested only by CMM). CONCLUSIONS: Both methods could be implemented as rapid diagnostic tools to detect drug-resistant isolates in clinical practice.
Fil: Morcillo, N.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giulio, B.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
description SETTING: Dr Cetrángolo Hospital, Buenos Aires Province, Argentina. OBJECTIVE: Evaluation of the BACTEC? Mycobacteria Growth Indicator Tube (MGIT)? 960 system and the colorimetric-based method (CMM) for fi rst- and second line drug susceptibility testing (FL-DST, SL-DST) against Mycobacterium tuberculosis. DESIGN: FL-DST was studied using SIRE MGIT 960. Minimal inhibitory concentrations (MICs) for isoniazid (INH), streptomycin, rifampicin (RMP), ethambutol (EMB) and levofl oxacin (LVX) were also determined by CMM using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT). MICs for amikacin (AMK), kanamycin (KM), capreomycin (CPM), ethionamide (ETH), cycloserine, ofl oxacin (OFX), linezolide (LZ) and moxifl oxacin (MFX) were determined on 94 multidrug resistant M. tuberculosis isolates by MGIT 960 and CMM. Statistical methods were applied to define drug susceptible and drug-resistant isolates on the basis of the comparison between results obtained by gold standards. RESULTS: A total of 1626 clinical isolates were studied. Critical drug concentrations could be defi ned in less than 10 days for both CMM and MGIT 960. CMM was cheaper but more laborious than MGIT 960. The highest performances of both methods were achieved for AMK, RMP, OFX, LZ and MFX, followed by INH, ETH, KM, CPM and LVX (tested only by CMM). CONCLUSIONS: Both methods could be implemented as rapid diagnostic tools to detect drug-resistant isolates in clinical practice.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190897
Morcillo, N.; Imperiale, Belén Rocío; Di Giulio, B.; Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 14; 9; 9-2010; 1169-1175
1027-3719
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190897
identifier_str_mv Morcillo, N.; Imperiale, Belén Rocío; Di Giulio, B.; Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 14; 9; 9-2010; 1169-1175
1027-3719
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2010/00000014/00000009/art00017;jsessionid=6m97djap025ii.x-ic-live-02
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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