Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium

Autores
Carretero, Maria Ignacia; Giuliano, S. M.; Arraztoa, Claudia Cecilia; Santa Cruz, R.; Fumuso, Fernanda Gabriela; Neild, Debora Margarita
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seminal plasma (SP) of South American Camelids could interfere with the interaction of spermatozoa with the extenders; therefore it becomes necessary to improve semen management using enzymatic treatment. Our objective was to compare two cooling protocols for llama semen. Twelve ejaculates were incubated in 0.1% collagenase and then were divided into two aliquots. One was extended in lactose and egg yolk (LEY) (Protocol A: collagenase and SP present). The other aliquot was centrifuged, and the pellet was resuspended in LEY (Protocol B: collagenase and SP absent). Both samples were maintained at 5°C during 24 hr. Routine and DNA evaluations were carried out in raw and cooled semen. Both cooling protocols maintained sperm viability, membrane function and DNA fragmentation, with Protocol A showing a significantly lowered total and progressive motility (p < .05) and Protocol B showing a significant increase in chromatin decondensation (p < .05). Protocol A avoids centrifugation, reducing processing times and making application in the field simpler. However, as neither protocol showed a significant superiority over the other, studies should be carried out in vivo to evaluate the effect on pregnancy rates of the presence of collagenase and SP in semen samples prior to either cooling or freeze-thawing.
Fil: Carretero, Maria Ignacia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Giuliano, S. M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santa Cruz, R.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Materia
LLAMA
COLAGENASA
REFIGERADO
SEMEN
PLASMA SEMINAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112412
Acceder
id CONICETDig_2fe37ad465b0f33414bc686bfadd6772
oai_identifier_str oai:ri.conicet.gov.ar:11336/112412
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the mediumCarretero, Maria IgnaciaGiuliano, S. M.Arraztoa, Claudia CeciliaSanta Cruz, R.Fumuso, Fernanda GabrielaNeild, Debora MargaritaLLAMACOLAGENASAREFIGERADOSEMENPLASMA SEMINALhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Seminal plasma (SP) of South American Camelids could interfere with the interaction of spermatozoa with the extenders; therefore it becomes necessary to improve semen management using enzymatic treatment. Our objective was to compare two cooling protocols for llama semen. Twelve ejaculates were incubated in 0.1% collagenase and then were divided into two aliquots. One was extended in lactose and egg yolk (LEY) (Protocol A: collagenase and SP present). The other aliquot was centrifuged, and the pellet was resuspended in LEY (Protocol B: collagenase and SP absent). Both samples were maintained at 5°C during 24 hr. Routine and DNA evaluations were carried out in raw and cooled semen. Both cooling protocols maintained sperm viability, membrane function and DNA fragmentation, with Protocol A showing a significantly lowered total and progressive motility (p < .05) and Protocol B showing a significant increase in chromatin decondensation (p < .05). Protocol A avoids centrifugation, reducing processing times and making application in the field simpler. However, as neither protocol showed a significant superiority over the other, studies should be carried out in vivo to evaluate the effect on pregnancy rates of the presence of collagenase and SP in semen samples prior to either cooling or freeze-thawing.Fil: Carretero, Maria Ignacia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Giuliano, S. M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Santa Cruz, R.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; ArgentinaFil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaWiley Blackwell Publishing, Inc2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112412Carretero, Maria Ignacia; Giuliano, S. M.; Arraztoa, Claudia Cecilia; Santa Cruz, R.; Fumuso, Fernanda Gabriela; et al.; Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium; Wiley Blackwell Publishing, Inc; Andrologia; 48; 6-2017; 1-80303-4569CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.1111/and.12691info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12691info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:25Zoai:ri.conicet.gov.ar:11336/112412instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:25.529CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
title Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
spellingShingle Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
Carretero, Maria Ignacia
LLAMA
COLAGENASA
REFIGERADO
SEMEN
PLASMA SEMINAL
title_short Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
title_full Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
title_fullStr Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
title_full_unstemmed Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
title_sort Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium
dc.creator.none.fl_str_mv Carretero, Maria Ignacia
Giuliano, S. M.
Arraztoa, Claudia Cecilia
Santa Cruz, R.
Fumuso, Fernanda Gabriela
Neild, Debora Margarita
author Carretero, Maria Ignacia
author_facet Carretero, Maria Ignacia
Giuliano, S. M.
Arraztoa, Claudia Cecilia
Santa Cruz, R.
Fumuso, Fernanda Gabriela
Neild, Debora Margarita
author_role author
author2 Giuliano, S. M.
Arraztoa, Claudia Cecilia
Santa Cruz, R.
Fumuso, Fernanda Gabriela
Neild, Debora Margarita
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv LLAMA
COLAGENASA
REFIGERADO
SEMEN
PLASMA SEMINAL
topic LLAMA
COLAGENASA
REFIGERADO
SEMEN
PLASMA SEMINAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Seminal plasma (SP) of South American Camelids could interfere with the interaction of spermatozoa with the extenders; therefore it becomes necessary to improve semen management using enzymatic treatment. Our objective was to compare two cooling protocols for llama semen. Twelve ejaculates were incubated in 0.1% collagenase and then were divided into two aliquots. One was extended in lactose and egg yolk (LEY) (Protocol A: collagenase and SP present). The other aliquot was centrifuged, and the pellet was resuspended in LEY (Protocol B: collagenase and SP absent). Both samples were maintained at 5°C during 24 hr. Routine and DNA evaluations were carried out in raw and cooled semen. Both cooling protocols maintained sperm viability, membrane function and DNA fragmentation, with Protocol A showing a significantly lowered total and progressive motility (p < .05) and Protocol B showing a significant increase in chromatin decondensation (p < .05). Protocol A avoids centrifugation, reducing processing times and making application in the field simpler. However, as neither protocol showed a significant superiority over the other, studies should be carried out in vivo to evaluate the effect on pregnancy rates of the presence of collagenase and SP in semen samples prior to either cooling or freeze-thawing.
Fil: Carretero, Maria Ignacia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Giuliano, S. M.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santa Cruz, R.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina
Fil: Fumuso, Fernanda Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
description Seminal plasma (SP) of South American Camelids could interfere with the interaction of spermatozoa with the extenders; therefore it becomes necessary to improve semen management using enzymatic treatment. Our objective was to compare two cooling protocols for llama semen. Twelve ejaculates were incubated in 0.1% collagenase and then were divided into two aliquots. One was extended in lactose and egg yolk (LEY) (Protocol A: collagenase and SP present). The other aliquot was centrifuged, and the pellet was resuspended in LEY (Protocol B: collagenase and SP absent). Both samples were maintained at 5°C during 24 hr. Routine and DNA evaluations were carried out in raw and cooled semen. Both cooling protocols maintained sperm viability, membrane function and DNA fragmentation, with Protocol A showing a significantly lowered total and progressive motility (p < .05) and Protocol B showing a significant increase in chromatin decondensation (p < .05). Protocol A avoids centrifugation, reducing processing times and making application in the field simpler. However, as neither protocol showed a significant superiority over the other, studies should be carried out in vivo to evaluate the effect on pregnancy rates of the presence of collagenase and SP in semen samples prior to either cooling or freeze-thawing.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112412
Carretero, Maria Ignacia; Giuliano, S. M.; Arraztoa, Claudia Cecilia; Santa Cruz, R.; Fumuso, Fernanda Gabriela; et al.; Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium; Wiley Blackwell Publishing, Inc; Andrologia; 48; 6-2017; 1-8
0303-4569
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112412
identifier_str_mv Carretero, Maria Ignacia; Giuliano, S. M.; Arraztoa, Claudia Cecilia; Santa Cruz, R.; Fumuso, Fernanda Gabriela; et al.; Comparison of two cooling protocols for llama semen: with and without collagenase and seminal plasma in the medium; Wiley Blackwell Publishing, Inc; Andrologia; 48; 6-2017; 1-8
0303-4569
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.1111/and.12691
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12691
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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