Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans

Autores
Veuthey, Tania Vanesa; Giunti, Sebastián; Alkema, Mark; de Rosa, Maria Jose; Rayes, Diego Hernán
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The ability of organisms to appropriately respond to physiological and environmental challenges requires conserved mechanisms, known as the “stress response”. The nervous system plays a key role in the coordination of systemic stress response. Recently, we demonstrated that in basal conditions tyramine released from the RIM neuron inhibits stress response in worms. Moreover, we demonstrated that expression of the GPCR TYRA-3 in the intestine is essential for this inhibition. When exposed to a stressor, such as heat, oxidative stress or lack of nutrients, tyramine signalling from the RIM should be stop to allow the animal to respond to these stressful conditions. We also observed that the insulin receptor DAF-2 is involved in this neural regulation of stress response, suggesting the compromise of insulin/insulin-like growth factor signaling (IIS) pathway. However, which are the signals that connect the intestine, where tyra-3 is expressed, with DAF-2 in several other cells are completely unknown. With this aim, we analyzed expression pattern of some of the 40 insulin like-peptides (ILPs) of C. elegans. We found that INS-3 and INS-7 co-localize with TYRA-3 in intestine. Moreover, expression of ins-7 in RIM neuron has been previously described. We also observed that null mutants of these ILPs are as resistant to thermal and oxidative stress as tdc-1 (incapable of sinthetizing tyramine) and tyra3 null mutants. We generated double null mutants of tdc-1 and tyra-3 with ins-3 and -7 in order to study epistatic relationships. Moreover, we are analizing the effects of exogenous tyramine on ins-3 and -7 expression. This study will contribute to understand molecular mechanisms involved in neuronal regulation of stress response in a multicellular organism.
Fil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giunti, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Alkema, Mark. University of Massachussets; Estados Unidos
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Expanding Caenorhabditis elegans research: First Latin American Worm Meeting
Montevideo
Uruguay
Latin American Society for Developmental Biology
Instituto Pasteur Montevideo
Materia
C. ELEGANS
INSULINE
STRESS RESPONSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238903
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegansVeuthey, Tania VanesaGiunti, SebastiánAlkema, Markde Rosa, Maria JoseRayes, Diego HernánC. ELEGANSINSULINESTRESS RESPONSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ability of organisms to appropriately respond to physiological and environmental challenges requires conserved mechanisms, known as the “stress response”. The nervous system plays a key role in the coordination of systemic stress response. Recently, we demonstrated that in basal conditions tyramine released from the RIM neuron inhibits stress response in worms. Moreover, we demonstrated that expression of the GPCR TYRA-3 in the intestine is essential for this inhibition. When exposed to a stressor, such as heat, oxidative stress or lack of nutrients, tyramine signalling from the RIM should be stop to allow the animal to respond to these stressful conditions. We also observed that the insulin receptor DAF-2 is involved in this neural regulation of stress response, suggesting the compromise of insulin/insulin-like growth factor signaling (IIS) pathway. However, which are the signals that connect the intestine, where tyra-3 is expressed, with DAF-2 in several other cells are completely unknown. With this aim, we analyzed expression pattern of some of the 40 insulin like-peptides (ILPs) of C. elegans. We found that INS-3 and INS-7 co-localize with TYRA-3 in intestine. Moreover, expression of ins-7 in RIM neuron has been previously described. We also observed that null mutants of these ILPs are as resistant to thermal and oxidative stress as tdc-1 (incapable of sinthetizing tyramine) and tyra3 null mutants. We generated double null mutants of tdc-1 and tyra-3 with ins-3 and -7 in order to study epistatic relationships. Moreover, we are analizing the effects of exogenous tyramine on ins-3 and -7 expression. This study will contribute to understand molecular mechanisms involved in neuronal regulation of stress response in a multicellular organism.Fil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Giunti, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Alkema, Mark. University of Massachussets; Estados UnidosFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaExpanding Caenorhabditis elegans research: First Latin American Worm MeetingMontevideoUruguayLatin American Society for Developmental BiologyInstituto Pasteur MontevideoInstituto Pasteur Montevideo2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238903Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans; Expanding Caenorhabditis elegans research: First Latin American Worm Meeting; Montevideo; Uruguay; 2017; 44-45CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:49Zoai:ri.conicet.gov.ar:11336/238903instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:49.872CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
title Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
spellingShingle Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
Veuthey, Tania Vanesa
C. ELEGANS
INSULINE
STRESS RESPONSE
title_short Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
title_full Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
title_fullStr Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
title_full_unstemmed Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
title_sort Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans
dc.creator.none.fl_str_mv Veuthey, Tania Vanesa
Giunti, Sebastián
Alkema, Mark
de Rosa, Maria Jose
Rayes, Diego Hernán
author Veuthey, Tania Vanesa
author_facet Veuthey, Tania Vanesa
Giunti, Sebastián
Alkema, Mark
de Rosa, Maria Jose
Rayes, Diego Hernán
author_role author
author2 Giunti, Sebastián
Alkema, Mark
de Rosa, Maria Jose
Rayes, Diego Hernán
author2_role author
author
author
author
dc.subject.none.fl_str_mv C. ELEGANS
INSULINE
STRESS RESPONSE
topic C. ELEGANS
INSULINE
STRESS RESPONSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ability of organisms to appropriately respond to physiological and environmental challenges requires conserved mechanisms, known as the “stress response”. The nervous system plays a key role in the coordination of systemic stress response. Recently, we demonstrated that in basal conditions tyramine released from the RIM neuron inhibits stress response in worms. Moreover, we demonstrated that expression of the GPCR TYRA-3 in the intestine is essential for this inhibition. When exposed to a stressor, such as heat, oxidative stress or lack of nutrients, tyramine signalling from the RIM should be stop to allow the animal to respond to these stressful conditions. We also observed that the insulin receptor DAF-2 is involved in this neural regulation of stress response, suggesting the compromise of insulin/insulin-like growth factor signaling (IIS) pathway. However, which are the signals that connect the intestine, where tyra-3 is expressed, with DAF-2 in several other cells are completely unknown. With this aim, we analyzed expression pattern of some of the 40 insulin like-peptides (ILPs) of C. elegans. We found that INS-3 and INS-7 co-localize with TYRA-3 in intestine. Moreover, expression of ins-7 in RIM neuron has been previously described. We also observed that null mutants of these ILPs are as resistant to thermal and oxidative stress as tdc-1 (incapable of sinthetizing tyramine) and tyra3 null mutants. We generated double null mutants of tdc-1 and tyra-3 with ins-3 and -7 in order to study epistatic relationships. Moreover, we are analizing the effects of exogenous tyramine on ins-3 and -7 expression. This study will contribute to understand molecular mechanisms involved in neuronal regulation of stress response in a multicellular organism.
Fil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giunti, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Alkema, Mark. University of Massachussets; Estados Unidos
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Expanding Caenorhabditis elegans research: First Latin American Worm Meeting
Montevideo
Uruguay
Latin American Society for Developmental Biology
Instituto Pasteur Montevideo
description The ability of organisms to appropriately respond to physiological and environmental challenges requires conserved mechanisms, known as the “stress response”. The nervous system plays a key role in the coordination of systemic stress response. Recently, we demonstrated that in basal conditions tyramine released from the RIM neuron inhibits stress response in worms. Moreover, we demonstrated that expression of the GPCR TYRA-3 in the intestine is essential for this inhibition. When exposed to a stressor, such as heat, oxidative stress or lack of nutrients, tyramine signalling from the RIM should be stop to allow the animal to respond to these stressful conditions. We also observed that the insulin receptor DAF-2 is involved in this neural regulation of stress response, suggesting the compromise of insulin/insulin-like growth factor signaling (IIS) pathway. However, which are the signals that connect the intestine, where tyra-3 is expressed, with DAF-2 in several other cells are completely unknown. With this aim, we analyzed expression pattern of some of the 40 insulin like-peptides (ILPs) of C. elegans. We found that INS-3 and INS-7 co-localize with TYRA-3 in intestine. Moreover, expression of ins-7 in RIM neuron has been previously described. We also observed that null mutants of these ILPs are as resistant to thermal and oxidative stress as tdc-1 (incapable of sinthetizing tyramine) and tyra3 null mutants. We generated double null mutants of tdc-1 and tyra-3 with ins-3 and -7 in order to study epistatic relationships. Moreover, we are analizing the effects of exogenous tyramine on ins-3 and -7 expression. This study will contribute to understand molecular mechanisms involved in neuronal regulation of stress response in a multicellular organism.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238903
Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans; Expanding Caenorhabditis elegans research: First Latin American Worm Meeting; Montevideo; Uruguay; 2017; 44-45
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238903
identifier_str_mv Screening of insuline like peptides (ILPs) involved in the neural coordination of stress response in c.elegans; Expanding Caenorhabditis elegans research: First Latin American Worm Meeting; Montevideo; Uruguay; 2017; 44-45
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Instituto Pasteur Montevideo
publisher.none.fl_str_mv Instituto Pasteur Montevideo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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