Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility

Autores
Rivera, Bárbara; Aroca, Claudia; González, Braian; Guzmán, Neftalí; Letelier, Pablo; Uribe, Pamela; Fornes, Miguel Walter; Villegas, Juana Valentina; Boguen, Rodrigo
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Male infertility is often linked to sperm quality issues; however, the mechanisms behind these alterations remain unclear in certain contexts. This study investigates the impact of anomalous Rho GTPase activation-a process triggered by bacterial toxins-on human sperm structure and function. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions. The results showed increased RhoA GTPase activity in non-capacitating conditions, without affecting viability or mitochondrial membrane potential. However, progressive motility decreased across both conditions, while non-progressive motility and acrosome reaction rates increased. Additionally, intracellular calcium levels rose exclusively in non-capacitating conditions. Structural analysis revealed an increase in abnormal sperm morphology, particularly vacuoles in the sperm head. These findings highlight that anomalous Rho GTPase activation disrupts essential processes like motility and capacitation, which are crucial for successful fertilization. This study provides novel insights into how bacterial infections may induce sperm damage, proposing that Rho GTPase activity could serve as a biomarker for evaluating sperm quality in cases of infertility linked to urogenital infections. Understanding these mechanisms may improve diagnostic and therapeutic approaches for male infertility associated with bacterial pathogens. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions.
Fil: Rivera, Bárbara. Universidad Catolica de Temuco.; Chile
Fil: Aroca, Claudia. Universidad Catolica de Temuco.; Chile
Fil: González, Braian. Universidad Catolica de Temuco.; Chile
Fil: Guzmán, Neftalí. Universidad Catolica de Temuco.; Chile
Fil: Letelier, Pablo. Universidad Catolica de Temuco.; Chile
Fil: Uribe, Pamela. Universidad de La Frontera; Chile
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Villegas, Juana Valentina. Universidad de La Frontera; Chile
Fil: Boguen, Rodrigo. Universidad Catolica de Temuco.; Chile
Materia
cytotoxic necrotizing factor-1
human spermatozoa
Rho GTPases
acrosome reaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276818
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276818
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of InfertilityRivera, BárbaraAroca, ClaudiaGonzález, BraianGuzmán, NeftalíLetelier, PabloUribe, PamelaFornes, Miguel WalterVillegas, Juana ValentinaBoguen, Rodrigocytotoxic necrotizing factor-1human spermatozoaRho GTPasesacrosome reactionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Male infertility is often linked to sperm quality issues; however, the mechanisms behind these alterations remain unclear in certain contexts. This study investigates the impact of anomalous Rho GTPase activation-a process triggered by bacterial toxins-on human sperm structure and function. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions. The results showed increased RhoA GTPase activity in non-capacitating conditions, without affecting viability or mitochondrial membrane potential. However, progressive motility decreased across both conditions, while non-progressive motility and acrosome reaction rates increased. Additionally, intracellular calcium levels rose exclusively in non-capacitating conditions. Structural analysis revealed an increase in abnormal sperm morphology, particularly vacuoles in the sperm head. These findings highlight that anomalous Rho GTPase activation disrupts essential processes like motility and capacitation, which are crucial for successful fertilization. This study provides novel insights into how bacterial infections may induce sperm damage, proposing that Rho GTPase activity could serve as a biomarker for evaluating sperm quality in cases of infertility linked to urogenital infections. Understanding these mechanisms may improve diagnostic and therapeutic approaches for male infertility associated with bacterial pathogens. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions.Fil: Rivera, Bárbara. Universidad Catolica de Temuco.; ChileFil: Aroca, Claudia. Universidad Catolica de Temuco.; ChileFil: González, Braian. Universidad Catolica de Temuco.; ChileFil: Guzmán, Neftalí. Universidad Catolica de Temuco.; ChileFil: Letelier, Pablo. Universidad Catolica de Temuco.; ChileFil: Uribe, Pamela. Universidad de La Frontera; ChileFil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Villegas, Juana Valentina. Universidad de La Frontera; ChileFil: Boguen, Rodrigo. Universidad Catolica de Temuco.; ChileMolecular Diversity Preservation International2025-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276818Rivera, Bárbara; Aroca, Claudia; González, Braian; Guzmán, Neftalí; Letelier, Pablo; et al.; Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 8; 4-2025; 1-141422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/8/3783info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26083783info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:59:05Zoai:ri.conicet.gov.ar:11336/276818instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:59:05.788CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
title Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
spellingShingle Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
Rivera, Bárbara
cytotoxic necrotizing factor-1
human spermatozoa
Rho GTPases
acrosome reaction
title_short Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
title_full Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
title_fullStr Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
title_full_unstemmed Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
title_sort Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility
dc.creator.none.fl_str_mv Rivera, Bárbara
Aroca, Claudia
González, Braian
Guzmán, Neftalí
Letelier, Pablo
Uribe, Pamela
Fornes, Miguel Walter
Villegas, Juana Valentina
Boguen, Rodrigo
author Rivera, Bárbara
author_facet Rivera, Bárbara
Aroca, Claudia
González, Braian
Guzmán, Neftalí
Letelier, Pablo
Uribe, Pamela
Fornes, Miguel Walter
Villegas, Juana Valentina
Boguen, Rodrigo
author_role author
author2 Aroca, Claudia
González, Braian
Guzmán, Neftalí
Letelier, Pablo
Uribe, Pamela
Fornes, Miguel Walter
Villegas, Juana Valentina
Boguen, Rodrigo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cytotoxic necrotizing factor-1
human spermatozoa
Rho GTPases
acrosome reaction
topic cytotoxic necrotizing factor-1
human spermatozoa
Rho GTPases
acrosome reaction
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Male infertility is often linked to sperm quality issues; however, the mechanisms behind these alterations remain unclear in certain contexts. This study investigates the impact of anomalous Rho GTPase activation-a process triggered by bacterial toxins-on human sperm structure and function. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions. The results showed increased RhoA GTPase activity in non-capacitating conditions, without affecting viability or mitochondrial membrane potential. However, progressive motility decreased across both conditions, while non-progressive motility and acrosome reaction rates increased. Additionally, intracellular calcium levels rose exclusively in non-capacitating conditions. Structural analysis revealed an increase in abnormal sperm morphology, particularly vacuoles in the sperm head. These findings highlight that anomalous Rho GTPase activation disrupts essential processes like motility and capacitation, which are crucial for successful fertilization. This study provides novel insights into how bacterial infections may induce sperm damage, proposing that Rho GTPase activity could serve as a biomarker for evaluating sperm quality in cases of infertility linked to urogenital infections. Understanding these mechanisms may improve diagnostic and therapeutic approaches for male infertility associated with bacterial pathogens. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions.
Fil: Rivera, Bárbara. Universidad Catolica de Temuco.; Chile
Fil: Aroca, Claudia. Universidad Catolica de Temuco.; Chile
Fil: González, Braian. Universidad Catolica de Temuco.; Chile
Fil: Guzmán, Neftalí. Universidad Catolica de Temuco.; Chile
Fil: Letelier, Pablo. Universidad Catolica de Temuco.; Chile
Fil: Uribe, Pamela. Universidad de La Frontera; Chile
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Villegas, Juana Valentina. Universidad de La Frontera; Chile
Fil: Boguen, Rodrigo. Universidad Catolica de Temuco.; Chile
description Male infertility is often linked to sperm quality issues; however, the mechanisms behind these alterations remain unclear in certain contexts. This study investigates the impact of anomalous Rho GTPase activation-a process triggered by bacterial toxins-on human sperm structure and function. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions. The results showed increased RhoA GTPase activity in non-capacitating conditions, without affecting viability or mitochondrial membrane potential. However, progressive motility decreased across both conditions, while non-progressive motility and acrosome reaction rates increased. Additionally, intracellular calcium levels rose exclusively in non-capacitating conditions. Structural analysis revealed an increase in abnormal sperm morphology, particularly vacuoles in the sperm head. These findings highlight that anomalous Rho GTPase activation disrupts essential processes like motility and capacitation, which are crucial for successful fertilization. This study provides novel insights into how bacterial infections may induce sperm damage, proposing that Rho GTPase activity could serve as a biomarker for evaluating sperm quality in cases of infertility linked to urogenital infections. Understanding these mechanisms may improve diagnostic and therapeutic approaches for male infertility associated with bacterial pathogens. Human spermatozoa were exposed in vitro to a Rho GTPase activator derived from Escherichia coli under both capacitating and non-capacitating conditions.
publishDate 2025
dc.date.none.fl_str_mv 2025-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276818
Rivera, Bárbara; Aroca, Claudia; González, Braian; Guzmán, Neftalí; Letelier, Pablo; et al.; Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 8; 4-2025; 1-14
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276818
identifier_str_mv Rivera, Bárbara; Aroca, Claudia; González, Braian; Guzmán, Neftalí; Letelier, Pablo; et al.; Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 8; 4-2025; 1-14
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/8/3783
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26083783
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1852335493593694208
score 12.952241

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