Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride

Autores
Lima Leite, Aline; Lobo, Janete Gualiume Vaz Madureira; Pereira, Heloísa Aparecida Barbosa Da Silva; Fernandes, Mileni Silva; Martini, Tatiani; Zucki, Fernanda; Hissako Sumida, Dóris; Rigalli, Alfredo; Rabelo Buzalaf, Marilia Afonso
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Administration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin- induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved.
Fil: Lima Leite, Aline. Universidade Federal do São Carlos; Brasil. Universidade de Sao Paulo; Brasil
Fil: Lobo, Janete Gualiume Vaz Madureira. Universidade de Sao Paulo; Brasil
Fil: Pereira, Heloísa Aparecida Barbosa Da Silva. Universidade Federal do São Carlos; Brasil
Fil: Fernandes, Mileni Silva. Universidade Federal do São Carlos; Brasil
Fil: Martini, Tatiani. Universidade de Sao Paulo; Brasil
Fil: Zucki, Fernanda. Universidade de Sao Paulo; Brasil
Fil: Hissako Sumida, Dóris. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Rabelo Buzalaf, Marilia Afonso. Universidade de Sao Paulo; Brasil
Materia
FLUORIDE
PROTEOMICS
INSULIN
INSULIN RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95163
Acceder
id CONICETDig_2faf63fdfc87a9fe8118b4e99154740b
oai_identifier_str oai:ri.conicet.gov.ar:11336/95163
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluorideLima Leite, AlineLobo, Janete Gualiume Vaz MadureiraPereira, Heloísa Aparecida Barbosa Da SilvaFernandes, Mileni SilvaMartini, TatianiZucki, FernandaHissako Sumida, DórisRigalli, AlfredoRabelo Buzalaf, Marilia AfonsoFLUORIDEPROTEOMICSINSULININSULIN RESISTANCEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Administration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin- induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved.Fil: Lima Leite, Aline. Universidade Federal do São Carlos; Brasil. Universidade de Sao Paulo; BrasilFil: Lobo, Janete Gualiume Vaz Madureira. Universidade de Sao Paulo; BrasilFil: Pereira, Heloísa Aparecida Barbosa Da Silva. Universidade Federal do São Carlos; BrasilFil: Fernandes, Mileni Silva. Universidade Federal do São Carlos; BrasilFil: Martini, Tatiani. Universidade de Sao Paulo; BrasilFil: Zucki, Fernanda. Universidade de Sao Paulo; BrasilFil: Hissako Sumida, Dóris. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Rabelo Buzalaf, Marilia Afonso. Universidade de Sao Paulo; BrasilPublic Library of Science2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95163Lima Leite, Aline; Lobo, Janete Gualiume Vaz Madureira; Pereira, Heloísa Aparecida Barbosa Da Silva; Fernandes, Mileni Silva; Martini, Tatiani; et al.; Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride; Public Library of Science; Plos One; 9; 8; 7-2014; 106646-1066561932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article/comments?id=10.1371/journal.pone.0106646info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0106646info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:34:45Zoai:ri.conicet.gov.ar:11336/95163instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:34:46.041CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
title Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
spellingShingle Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
Lima Leite, Aline
FLUORIDE
PROTEOMICS
INSULIN
INSULIN RESISTANCE
title_short Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
title_full Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
title_fullStr Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
title_full_unstemmed Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
title_sort Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride
dc.creator.none.fl_str_mv Lima Leite, Aline
Lobo, Janete Gualiume Vaz Madureira
Pereira, Heloísa Aparecida Barbosa Da Silva
Fernandes, Mileni Silva
Martini, Tatiani
Zucki, Fernanda
Hissako Sumida, Dóris
Rigalli, Alfredo
Rabelo Buzalaf, Marilia Afonso
author Lima Leite, Aline
author_facet Lima Leite, Aline
Lobo, Janete Gualiume Vaz Madureira
Pereira, Heloísa Aparecida Barbosa Da Silva
Fernandes, Mileni Silva
Martini, Tatiani
Zucki, Fernanda
Hissako Sumida, Dóris
Rigalli, Alfredo
Rabelo Buzalaf, Marilia Afonso
author_role author
author2 Lobo, Janete Gualiume Vaz Madureira
Pereira, Heloísa Aparecida Barbosa Da Silva
Fernandes, Mileni Silva
Martini, Tatiani
Zucki, Fernanda
Hissako Sumida, Dóris
Rigalli, Alfredo
Rabelo Buzalaf, Marilia Afonso
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FLUORIDE
PROTEOMICS
INSULIN
INSULIN RESISTANCE
topic FLUORIDE
PROTEOMICS
INSULIN
INSULIN RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Administration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin- induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved.
Fil: Lima Leite, Aline. Universidade Federal do São Carlos; Brasil. Universidade de Sao Paulo; Brasil
Fil: Lobo, Janete Gualiume Vaz Madureira. Universidade de Sao Paulo; Brasil
Fil: Pereira, Heloísa Aparecida Barbosa Da Silva. Universidade Federal do São Carlos; Brasil
Fil: Fernandes, Mileni Silva. Universidade Federal do São Carlos; Brasil
Fil: Martini, Tatiani. Universidade de Sao Paulo; Brasil
Fil: Zucki, Fernanda. Universidade de Sao Paulo; Brasil
Fil: Hissako Sumida, Dóris. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Rabelo Buzalaf, Marilia Afonso. Universidade de Sao Paulo; Brasil
description Administration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin- induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95163
Lima Leite, Aline; Lobo, Janete Gualiume Vaz Madureira; Pereira, Heloísa Aparecida Barbosa Da Silva; Fernandes, Mileni Silva; Martini, Tatiani; et al.; Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride; Public Library of Science; Plos One; 9; 8; 7-2014; 106646-106656
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95163
identifier_str_mv Lima Leite, Aline; Lobo, Janete Gualiume Vaz Madureira; Pereira, Heloísa Aparecida Barbosa Da Silva; Fernandes, Mileni Silva; Martini, Tatiani; et al.; Proteomic analysis of gastrocnemius muscle in rats with streptozotocin-induced diabetes and chronically exposed to fluoride; Public Library of Science; Plos One; 9; 8; 7-2014; 106646-106656
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article/comments?id=10.1371/journal.pone.0106646
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0106646
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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