Tools to Study SUMO Conjugation in Caenorhabditis elegans

Autores
Pelisch, Federico Gaston; Hay, Ronald T.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cell biology of sumoylation has mostly been studied using transformed cultured cells and yeast. Inrecent years, genetic analysis has demonstrated important roles for sumoylation in the biology of C. elegans. Here, we expand the existing set of tools making it possible to address the role of sumoylation in thenematode C. elegans using a combination of genetics, imaging, and biochemistry. Most importantly, thedynamics of SUMO conjugation and deconjugation can be followed very precisely both in space and timewithin living worms. Additionally, the biochemistry of SUMO conjugation and deconjugation can beaddressed using recombinant purifi ed components of the C. elegans sumoylation machinery, including E3ligases and SUMO proteases. These tools and reagents will be useful to gain insights into the biologicalrole of SUMO in the context of a multicellular organism.
Fil: Pelisch, Federico Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. University of Dundee; Reino Unido
Fil: Hay, Ronald T.. University of Dundee; Reino Unido
Materia
Sumo
Caenorhabditis Elegans
Cell Cycle
Microscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45389
Acceder
id CONICETDig_2f42b87f711668f929a5c544ecf1ad7a
oai_identifier_str oai:ri.conicet.gov.ar:11336/45389
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tools to Study SUMO Conjugation in Caenorhabditis elegansPelisch, Federico GastonHay, Ronald T.SumoCaenorhabditis ElegansCell CycleMicroscopyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The cell biology of sumoylation has mostly been studied using transformed cultured cells and yeast. Inrecent years, genetic analysis has demonstrated important roles for sumoylation in the biology of C. elegans. Here, we expand the existing set of tools making it possible to address the role of sumoylation in thenematode C. elegans using a combination of genetics, imaging, and biochemistry. Most importantly, thedynamics of SUMO conjugation and deconjugation can be followed very precisely both in space and timewithin living worms. Additionally, the biochemistry of SUMO conjugation and deconjugation can beaddressed using recombinant purifi ed components of the C. elegans sumoylation machinery, including E3ligases and SUMO proteases. These tools and reagents will be useful to gain insights into the biologicalrole of SUMO in the context of a multicellular organism.Fil: Pelisch, Federico Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. University of Dundee; Reino UnidoFil: Hay, Ronald T.. University of Dundee; Reino UnidoSpringer2016-09-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45389Pelisch, Federico Gaston; Hay, Ronald T.; Tools to Study SUMO Conjugation in Caenorhabditis elegans; Springer; Methods in Molecular Biology; 1475; 6-9-2016; 233-2561064-3745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007/978-1-4939-6358-4_17info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-6358-4_17info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:38Zoai:ri.conicet.gov.ar:11336/45389instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:39.193CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tools to Study SUMO Conjugation in Caenorhabditis elegans
title Tools to Study SUMO Conjugation in Caenorhabditis elegans
spellingShingle Tools to Study SUMO Conjugation in Caenorhabditis elegans
Pelisch, Federico Gaston
Sumo
Caenorhabditis Elegans
Cell Cycle
Microscopy
title_short Tools to Study SUMO Conjugation in Caenorhabditis elegans
title_full Tools to Study SUMO Conjugation in Caenorhabditis elegans
title_fullStr Tools to Study SUMO Conjugation in Caenorhabditis elegans
title_full_unstemmed Tools to Study SUMO Conjugation in Caenorhabditis elegans
title_sort Tools to Study SUMO Conjugation in Caenorhabditis elegans
dc.creator.none.fl_str_mv Pelisch, Federico Gaston
Hay, Ronald T.
author Pelisch, Federico Gaston
author_facet Pelisch, Federico Gaston
Hay, Ronald T.
author_role author
author2 Hay, Ronald T.
author2_role author
dc.subject.none.fl_str_mv Sumo
Caenorhabditis Elegans
Cell Cycle
Microscopy
topic Sumo
Caenorhabditis Elegans
Cell Cycle
Microscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The cell biology of sumoylation has mostly been studied using transformed cultured cells and yeast. Inrecent years, genetic analysis has demonstrated important roles for sumoylation in the biology of C. elegans. Here, we expand the existing set of tools making it possible to address the role of sumoylation in thenematode C. elegans using a combination of genetics, imaging, and biochemistry. Most importantly, thedynamics of SUMO conjugation and deconjugation can be followed very precisely both in space and timewithin living worms. Additionally, the biochemistry of SUMO conjugation and deconjugation can beaddressed using recombinant purifi ed components of the C. elegans sumoylation machinery, including E3ligases and SUMO proteases. These tools and reagents will be useful to gain insights into the biologicalrole of SUMO in the context of a multicellular organism.
Fil: Pelisch, Federico Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. University of Dundee; Reino Unido
Fil: Hay, Ronald T.. University of Dundee; Reino Unido
description The cell biology of sumoylation has mostly been studied using transformed cultured cells and yeast. Inrecent years, genetic analysis has demonstrated important roles for sumoylation in the biology of C. elegans. Here, we expand the existing set of tools making it possible to address the role of sumoylation in thenematode C. elegans using a combination of genetics, imaging, and biochemistry. Most importantly, thedynamics of SUMO conjugation and deconjugation can be followed very precisely both in space and timewithin living worms. Additionally, the biochemistry of SUMO conjugation and deconjugation can beaddressed using recombinant purifi ed components of the C. elegans sumoylation machinery, including E3ligases and SUMO proteases. These tools and reagents will be useful to gain insights into the biologicalrole of SUMO in the context of a multicellular organism.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45389
Pelisch, Federico Gaston; Hay, Ronald T.; Tools to Study SUMO Conjugation in Caenorhabditis elegans; Springer; Methods in Molecular Biology; 1475; 6-9-2016; 233-256
1064-3745
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45389
identifier_str_mv Pelisch, Federico Gaston; Hay, Ronald T.; Tools to Study SUMO Conjugation in Caenorhabditis elegans; Springer; Methods in Molecular Biology; 1475; 6-9-2016; 233-256
1064-3745
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007/978-1-4939-6358-4_17
info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-6358-4_17
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613111849418752
score 13.070432

Publicaciones similares