Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective

Autores
Sarmiento Varon, Lindybeth; de Rosa, Javier Enrique; Fernandez, Pablo Mariano; Arabolaza, M. E.; Paoli, Bibiana Patricia; Vay, Carlos Alberto; Barberis, C. M.; Arana, Eloisa
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Obstructive sleep apnoea (OSAS) is a syndrome suffered by children with hypertrophied tonsils. We have previously demonstrated that these tonsils present a defective Breg compartment. Here, we extend those findings by evidencing the inflammatory cytokine pattern of tonsillar mononuclear cells (TMC) and investigating the grounds of such profile. OSAS TMC were the only cells used for this work. We showed the ability of Bcs to promote the loss of immune homeostatic control by promptly producing TNFα. Using FACS, we determined TNFα production by stimulated TMC in culture. Upon 24 hours, Bcs represented the majority of TNFα+ cells (52,4% ± SEM 4,2% CD20+/down cells vs 41,7% ± SEM 4,0% CD3+ cells, p < 0.05). Conversely, at the same time point, IL17 was produced primarily by CD4+ T cells (Th17) which comprised 90% of the IL17+ population. Also at 24 hs post stimulation, two thirds of the Th17 population (59% ± SEM 4%) co-expressed TNFα. Despite the pro-inflammatory profile displayed by TMC in culture, OSAS has long been considered of non-infectious etiology. We cultured the core tonsillar tissue of 31 children and identified 89 bacterial species by MALDI-TOF MS. The species identified had been previously found either causing ENT pathology or as harmless local flora, both situations in competent hosts. Pathogens differ from commensals in being able to penetrate the epithelial barriers. Hence, we performed fluorescence in situ hybridization (FISH) with a universal eubacterial (EUB338) probe followed by immune-fluorescence staining, on cryo-sections from excised tonsils. By confocal microscopy, we confirmed bacterial presence within the lymphoid compartment from OSAS biopsies. To conclude, while we cannot ascertain that the microorganisms detected in situ as well as through culture are the initiators of the ongoing inflammatory response characteristic of OSAS, the chronification of the process must be related to the evidenced bacterial spreading beyond the normal boundaries.
Fil: Sarmiento Varon, Lindybeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: de Rosa, Javier Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Fernandez, Pablo Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Arabolaza, M. E.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Paoli, Bibiana Patricia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Barberis, C. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Arana, Eloisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
tonsil
infection
colonization
osas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247615
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspectiveSarmiento Varon, Lindybethde Rosa, Javier EnriqueFernandez, Pablo MarianoArabolaza, M. E.Paoli, Bibiana PatriciaVay, Carlos AlbertoBarberis, C. M.Arana, Eloisatonsilinfectioncolonizationosashttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Obstructive sleep apnoea (OSAS) is a syndrome suffered by children with hypertrophied tonsils. We have previously demonstrated that these tonsils present a defective Breg compartment. Here, we extend those findings by evidencing the inflammatory cytokine pattern of tonsillar mononuclear cells (TMC) and investigating the grounds of such profile. OSAS TMC were the only cells used for this work. We showed the ability of Bcs to promote the loss of immune homeostatic control by promptly producing TNFα. Using FACS, we determined TNFα production by stimulated TMC in culture. Upon 24 hours, Bcs represented the majority of TNFα+ cells (52,4% ± SEM 4,2% CD20+/down cells vs 41,7% ± SEM 4,0% CD3+ cells, p < 0.05). Conversely, at the same time point, IL17 was produced primarily by CD4+ T cells (Th17) which comprised 90% of the IL17+ population. Also at 24 hs post stimulation, two thirds of the Th17 population (59% ± SEM 4%) co-expressed TNFα. Despite the pro-inflammatory profile displayed by TMC in culture, OSAS has long been considered of non-infectious etiology. We cultured the core tonsillar tissue of 31 children and identified 89 bacterial species by MALDI-TOF MS. The species identified had been previously found either causing ENT pathology or as harmless local flora, both situations in competent hosts. Pathogens differ from commensals in being able to penetrate the epithelial barriers. Hence, we performed fluorescence in situ hybridization (FISH) with a universal eubacterial (EUB338) probe followed by immune-fluorescence staining, on cryo-sections from excised tonsils. By confocal microscopy, we confirmed bacterial presence within the lymphoid compartment from OSAS biopsies. To conclude, while we cannot ascertain that the microorganisms detected in situ as well as through culture are the initiators of the ongoing inflammatory response characteristic of OSAS, the chronification of the process must be related to the evidenced bacterial spreading beyond the normal boundaries.Fil: Sarmiento Varon, Lindybeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: de Rosa, Javier Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Fernandez, Pablo Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Arabolaza, M. E.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Paoli, Bibiana Patricia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Barberis, C. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Arana, Eloisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/247615Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 1-50025-76801669-9106CONICET DigitalCONICETengNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:04Zoai:ri.conicet.gov.ar:11336/247615instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:04.536CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
title Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
spellingShingle Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
Sarmiento Varon, Lindybeth
tonsil
infection
colonization
osas
title_short Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
title_full Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
title_fullStr Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
title_full_unstemmed Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
title_sort Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective
dc.creator.none.fl_str_mv Sarmiento Varon, Lindybeth
de Rosa, Javier Enrique
Fernandez, Pablo Mariano
Arabolaza, M. E.
Paoli, Bibiana Patricia
Vay, Carlos Alberto
Barberis, C. M.
Arana, Eloisa
author Sarmiento Varon, Lindybeth
author_facet Sarmiento Varon, Lindybeth
de Rosa, Javier Enrique
Fernandez, Pablo Mariano
Arabolaza, M. E.
Paoli, Bibiana Patricia
Vay, Carlos Alberto
Barberis, C. M.
Arana, Eloisa
author_role author
author2 de Rosa, Javier Enrique
Fernandez, Pablo Mariano
Arabolaza, M. E.
Paoli, Bibiana Patricia
Vay, Carlos Alberto
Barberis, C. M.
Arana, Eloisa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv tonsil
infection
colonization
osas
topic tonsil
infection
colonization
osas
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Obstructive sleep apnoea (OSAS) is a syndrome suffered by children with hypertrophied tonsils. We have previously demonstrated that these tonsils present a defective Breg compartment. Here, we extend those findings by evidencing the inflammatory cytokine pattern of tonsillar mononuclear cells (TMC) and investigating the grounds of such profile. OSAS TMC were the only cells used for this work. We showed the ability of Bcs to promote the loss of immune homeostatic control by promptly producing TNFα. Using FACS, we determined TNFα production by stimulated TMC in culture. Upon 24 hours, Bcs represented the majority of TNFα+ cells (52,4% ± SEM 4,2% CD20+/down cells vs 41,7% ± SEM 4,0% CD3+ cells, p < 0.05). Conversely, at the same time point, IL17 was produced primarily by CD4+ T cells (Th17) which comprised 90% of the IL17+ population. Also at 24 hs post stimulation, two thirds of the Th17 population (59% ± SEM 4%) co-expressed TNFα. Despite the pro-inflammatory profile displayed by TMC in culture, OSAS has long been considered of non-infectious etiology. We cultured the core tonsillar tissue of 31 children and identified 89 bacterial species by MALDI-TOF MS. The species identified had been previously found either causing ENT pathology or as harmless local flora, both situations in competent hosts. Pathogens differ from commensals in being able to penetrate the epithelial barriers. Hence, we performed fluorescence in situ hybridization (FISH) with a universal eubacterial (EUB338) probe followed by immune-fluorescence staining, on cryo-sections from excised tonsils. By confocal microscopy, we confirmed bacterial presence within the lymphoid compartment from OSAS biopsies. To conclude, while we cannot ascertain that the microorganisms detected in situ as well as through culture are the initiators of the ongoing inflammatory response characteristic of OSAS, the chronification of the process must be related to the evidenced bacterial spreading beyond the normal boundaries.
Fil: Sarmiento Varon, Lindybeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: de Rosa, Javier Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Fernandez, Pablo Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Arabolaza, M. E.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Paoli, Bibiana Patricia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Barberis, C. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Arana, Eloisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Obstructive sleep apnoea (OSAS) is a syndrome suffered by children with hypertrophied tonsils. We have previously demonstrated that these tonsils present a defective Breg compartment. Here, we extend those findings by evidencing the inflammatory cytokine pattern of tonsillar mononuclear cells (TMC) and investigating the grounds of such profile. OSAS TMC were the only cells used for this work. We showed the ability of Bcs to promote the loss of immune homeostatic control by promptly producing TNFα. Using FACS, we determined TNFα production by stimulated TMC in culture. Upon 24 hours, Bcs represented the majority of TNFα+ cells (52,4% ± SEM 4,2% CD20+/down cells vs 41,7% ± SEM 4,0% CD3+ cells, p < 0.05). Conversely, at the same time point, IL17 was produced primarily by CD4+ T cells (Th17) which comprised 90% of the IL17+ population. Also at 24 hs post stimulation, two thirds of the Th17 population (59% ± SEM 4%) co-expressed TNFα. Despite the pro-inflammatory profile displayed by TMC in culture, OSAS has long been considered of non-infectious etiology. We cultured the core tonsillar tissue of 31 children and identified 89 bacterial species by MALDI-TOF MS. The species identified had been previously found either causing ENT pathology or as harmless local flora, both situations in competent hosts. Pathogens differ from commensals in being able to penetrate the epithelial barriers. Hence, we performed fluorescence in situ hybridization (FISH) with a universal eubacterial (EUB338) probe followed by immune-fluorescence staining, on cryo-sections from excised tonsils. By confocal microscopy, we confirmed bacterial presence within the lymphoid compartment from OSAS biopsies. To conclude, while we cannot ascertain that the microorganisms detected in situ as well as through culture are the initiators of the ongoing inflammatory response characteristic of OSAS, the chronification of the process must be related to the evidenced bacterial spreading beyond the normal boundaries.
publishDate 2020
dc.date.none.fl_str_mv 2020
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247615
Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 1-5
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247615
identifier_str_mv Inflammatory responses at the boundary between the host and the world beyond: the dilemma of infection versus colonization from a tonsillar perspective; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 1-5
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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