Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promoti...

Autores
Castro, Gerardo Daniel; Delgado de Layño, Aurora M. A.; Costantini, Martin Hernan; Castro, Jose Alberto
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We recently showed that mammary cytosolic xanthineoxidoreductase had the ability to bioactivate ethanol (EtOH) to acetaldehyde (AC) and free radicals. In thepresent study, we report that the microsomal fraction also biotransforms EtOH to AC. One pathway requires NADPH and the others do not. Both need oxygen. TheNADPH-dependent pathway is not inhibited by CO:O(2) (80:20) or SKF 525A and that excludes the participation of cytochrome P450. It is inhibited bydiethyldithiocarbamate (DDTC), sodium azide, and diphenyleneiodonium (DPI) butnot by desferrioxamine, which suggests a possible role of a non-ironcopper-requiring flavoenzyme. The process was partially inhibited bythiobenzamide (TBA), methylmercaptoimidazole (MMI), and nordihydroguaiaretic acid(NDG) but not by dapsone, aminotriazole, or indomethacin. These results suggestthe potential participation of flavine monooxygenase and of lipooxygenase or ofperoxidases/oxidases having similar characteristics but not of lactoperoxidase orcyclooxygenase. The pathway not requiring NADPH could also be partially inhibitedby DDTC, NDG, azide, DPI, and TBA or MMI but not by the other chemicals. Littleactivity proceeds under nitrogen. Oxidases or peroxidases might be involved. Noformation of 1-hydroxyethyl radicals was detected either in the presence orabsence of NADPH. The nature of the EtOH bioactivating enzymes involved remainsto be established. However, the fact remains that an activation of EtOH to AC wasfound in mammary tissue and could have a significant effect in some stages of theprocess of breast tumor promotion by EtOH.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Delgado de Layño, Aurora M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Materia
Breast Cancer
Alcohol Drinking
Breast Biotransformation of Alcohol
Acetaldehyde
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82747
Acceder
id CONICETDig_2e7e0a4264d276c36f548249e4985690
oai_identifier_str oai:ri.conicet.gov.ar:11336/82747
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotionCastro, Gerardo DanielDelgado de Layño, Aurora M. A.Costantini, Martin HernanCastro, Jose AlbertoBreast CancerAlcohol DrinkingBreast Biotransformation of AlcoholAcetaldehydehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We recently showed that mammary cytosolic xanthineoxidoreductase had the ability to bioactivate ethanol (EtOH) to acetaldehyde (AC) and free radicals. In thepresent study, we report that the microsomal fraction also biotransforms EtOH to AC. One pathway requires NADPH and the others do not. Both need oxygen. TheNADPH-dependent pathway is not inhibited by CO:O(2) (80:20) or SKF 525A and that excludes the participation of cytochrome P450. It is inhibited bydiethyldithiocarbamate (DDTC), sodium azide, and diphenyleneiodonium (DPI) butnot by desferrioxamine, which suggests a possible role of a non-ironcopper-requiring flavoenzyme. The process was partially inhibited bythiobenzamide (TBA), methylmercaptoimidazole (MMI), and nordihydroguaiaretic acid(NDG) but not by dapsone, aminotriazole, or indomethacin. These results suggestthe potential participation of flavine monooxygenase and of lipooxygenase or ofperoxidases/oxidases having similar characteristics but not of lactoperoxidase orcyclooxygenase. The pathway not requiring NADPH could also be partially inhibitedby DDTC, NDG, azide, DPI, and TBA or MMI but not by the other chemicals. Littleactivity proceeds under nitrogen. Oxidases or peroxidases might be involved. Noformation of 1-hydroxyethyl radicals was detected either in the presence orabsence of NADPH. The nature of the EtOH bioactivating enzymes involved remainsto be established. However, the fact remains that an activation of EtOH to AC wasfound in mammary tissue and could have a significant effect in some stages of theprocess of breast tumor promotion by EtOH.Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Delgado de Layño, Aurora M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaWiley2003-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82747Castro, Gerardo Daniel; Delgado de Layño, Aurora M. A.; Costantini, Martin Hernan; Castro, Jose Alberto; Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion; Wiley; Teratogenesis Carcinogenesis And Mutagenesis; 23; S1; 12-2003; 61-700270-3211CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/tcm.10060info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/tcm.10060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:01:42Zoai:ri.conicet.gov.ar:11336/82747instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:01:43.112CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
title Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
spellingShingle Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
Castro, Gerardo Daniel
Breast Cancer
Alcohol Drinking
Breast Biotransformation of Alcohol
Acetaldehyde
title_short Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
title_full Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
title_fullStr Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
title_full_unstemmed Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
title_sort Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion
dc.creator.none.fl_str_mv Castro, Gerardo Daniel
Delgado de Layño, Aurora M. A.
Costantini, Martin Hernan
Castro, Jose Alberto
author Castro, Gerardo Daniel
author_facet Castro, Gerardo Daniel
Delgado de Layño, Aurora M. A.
Costantini, Martin Hernan
Castro, Jose Alberto
author_role author
author2 Delgado de Layño, Aurora M. A.
Costantini, Martin Hernan
Castro, Jose Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Breast Cancer
Alcohol Drinking
Breast Biotransformation of Alcohol
Acetaldehyde
topic Breast Cancer
Alcohol Drinking
Breast Biotransformation of Alcohol
Acetaldehyde
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We recently showed that mammary cytosolic xanthineoxidoreductase had the ability to bioactivate ethanol (EtOH) to acetaldehyde (AC) and free radicals. In thepresent study, we report that the microsomal fraction also biotransforms EtOH to AC. One pathway requires NADPH and the others do not. Both need oxygen. TheNADPH-dependent pathway is not inhibited by CO:O(2) (80:20) or SKF 525A and that excludes the participation of cytochrome P450. It is inhibited bydiethyldithiocarbamate (DDTC), sodium azide, and diphenyleneiodonium (DPI) butnot by desferrioxamine, which suggests a possible role of a non-ironcopper-requiring flavoenzyme. The process was partially inhibited bythiobenzamide (TBA), methylmercaptoimidazole (MMI), and nordihydroguaiaretic acid(NDG) but not by dapsone, aminotriazole, or indomethacin. These results suggestthe potential participation of flavine monooxygenase and of lipooxygenase or ofperoxidases/oxidases having similar characteristics but not of lactoperoxidase orcyclooxygenase. The pathway not requiring NADPH could also be partially inhibitedby DDTC, NDG, azide, DPI, and TBA or MMI but not by the other chemicals. Littleactivity proceeds under nitrogen. Oxidases or peroxidases might be involved. Noformation of 1-hydroxyethyl radicals was detected either in the presence orabsence of NADPH. The nature of the EtOH bioactivating enzymes involved remainsto be established. However, the fact remains that an activation of EtOH to AC wasfound in mammary tissue and could have a significant effect in some stages of theprocess of breast tumor promotion by EtOH.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Delgado de Layño, Aurora M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
description We recently showed that mammary cytosolic xanthineoxidoreductase had the ability to bioactivate ethanol (EtOH) to acetaldehyde (AC) and free radicals. In thepresent study, we report that the microsomal fraction also biotransforms EtOH to AC. One pathway requires NADPH and the others do not. Both need oxygen. TheNADPH-dependent pathway is not inhibited by CO:O(2) (80:20) or SKF 525A and that excludes the participation of cytochrome P450. It is inhibited bydiethyldithiocarbamate (DDTC), sodium azide, and diphenyleneiodonium (DPI) butnot by desferrioxamine, which suggests a possible role of a non-ironcopper-requiring flavoenzyme. The process was partially inhibited bythiobenzamide (TBA), methylmercaptoimidazole (MMI), and nordihydroguaiaretic acid(NDG) but not by dapsone, aminotriazole, or indomethacin. These results suggestthe potential participation of flavine monooxygenase and of lipooxygenase or ofperoxidases/oxidases having similar characteristics but not of lactoperoxidase orcyclooxygenase. The pathway not requiring NADPH could also be partially inhibitedby DDTC, NDG, azide, DPI, and TBA or MMI but not by the other chemicals. Littleactivity proceeds under nitrogen. Oxidases or peroxidases might be involved. Noformation of 1-hydroxyethyl radicals was detected either in the presence orabsence of NADPH. The nature of the EtOH bioactivating enzymes involved remainsto be established. However, the fact remains that an activation of EtOH to AC wasfound in mammary tissue and could have a significant effect in some stages of theprocess of breast tumor promotion by EtOH.
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82747
Castro, Gerardo Daniel; Delgado de Layño, Aurora M. A.; Costantini, Martin Hernan; Castro, Jose Alberto; Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion; Wiley; Teratogenesis Carcinogenesis And Mutagenesis; 23; S1; 12-2003; 61-70
0270-3211
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82747
identifier_str_mv Castro, Gerardo Daniel; Delgado de Layño, Aurora M. A.; Costantini, Martin Hernan; Castro, Jose Alberto; Rat breast microsomal biotransformation of ethanol to acetaldehyde but not to free radicals: Its potential role in the association between alcohol drinking and breast tumor promotion; Wiley; Teratogenesis Carcinogenesis And Mutagenesis; 23; S1; 12-2003; 61-70
0270-3211
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/tcm.10060
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/tcm.10060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083158207889408
score 13.22299

Publicaciones similares