Electron-transfer processes induced by the triplet state of pterins in aqueous solutions

Autores
Dantola, Maria Laura; Vignoni, Mariana; González, Constanza; Lorente, Carolina; Vicendo, Patricia; Oliveros, Esther; Thomas, Andrés Héctor
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pterins (Pt) are heterocyclic compounds widespread in living systems. They participate in relevant biological processes, such as metabolic redox reactions, and can photoinduce the oxidation of biomolecules through electron transfer mechanisms. We have investigated the electron transfer pathways initiated by excited states of pterins. The study was performed with pterin (Ptr) and 6-methylpterin (Mep), selected as model compounds. The experiments were carried out in aqueous solutions under continuous UV irradiation, in the presence and in the absence of ethylenediaminetetraacetic acid (EDTA), used as an electron donor. The reactions were followed by UV/VIS spectrophotometry, HPLC and an enzymatic method for H2O2 determination. The formation of the superoxide anion (O2•‒) was investigated by electron paramagnetic resonance. The triplet excited states of Ptr and Mep are efficient electron acceptors, able to oxidize a Pt molecule in its ground state. The resulting radical anion (Pt•‒) reacts with dissolved O2 to yield O2•‒, regenerating the pterin. In the presence of EDTA, this reaction competes efficiently with the anaerobic reaction between Pt•- and EDTA•+, yielding the corresponding stable dihydroderivatives H2Pt. The effects of EDTA and dissolved O2 concentrations on the efficiencies of the different competing pathways were analyzed.
Fil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Vignoni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: González, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Lorente, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Vicendo, Patricia. Université Paul Sabatier; Francia
Fil: Oliveros, Esther. Université Paul Sabatier; Francia
Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Materia
Pterins
Photoinduced electron transfer
Superoxide anion
Free radicals
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/248536
Acceder
id CONICETDig_2e758fd38823b2aeed1546934564bb2f
oai_identifier_str oai:ri.conicet.gov.ar:11336/248536
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electron-transfer processes induced by the triplet state of pterins in aqueous solutionsDantola, Maria LauraVignoni, MarianaGonzález, ConstanzaLorente, CarolinaVicendo, PatriciaOliveros, EstherThomas, Andrés HéctorPterinsPhotoinduced electron transferSuperoxide anionFree radicalshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Pterins (Pt) are heterocyclic compounds widespread in living systems. They participate in relevant biological processes, such as metabolic redox reactions, and can photoinduce the oxidation of biomolecules through electron transfer mechanisms. We have investigated the electron transfer pathways initiated by excited states of pterins. The study was performed with pterin (Ptr) and 6-methylpterin (Mep), selected as model compounds. The experiments were carried out in aqueous solutions under continuous UV irradiation, in the presence and in the absence of ethylenediaminetetraacetic acid (EDTA), used as an electron donor. The reactions were followed by UV/VIS spectrophotometry, HPLC and an enzymatic method for H2O2 determination. The formation of the superoxide anion (O2•‒) was investigated by electron paramagnetic resonance. The triplet excited states of Ptr and Mep are efficient electron acceptors, able to oxidize a Pt molecule in its ground state. The resulting radical anion (Pt•‒) reacts with dissolved O2 to yield O2•‒, regenerating the pterin. In the presence of EDTA, this reaction competes efficiently with the anaerobic reaction between Pt•- and EDTA•+, yielding the corresponding stable dihydroderivatives H2Pt. The effects of EDTA and dissolved O2 concentrations on the efficiencies of the different competing pathways were analyzed.Fil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Vignoni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: González, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Lorente, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Vicendo, Patricia. Université Paul Sabatier; FranciaFil: Oliveros, Esther. Université Paul Sabatier; FranciaFil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaElsevier Science Inc.2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/248536Dantola, Maria Laura; Vignoni, Mariana; González, Constanza; Lorente, Carolina; Vicendo, Patricia; et al.; Electron-transfer processes induced by the triplet state of pterins in aqueous solutions; Elsevier Science Inc.; Free Radical Biology and Medicine; 49; 6; 9-2010; 1014-10220891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0891584910003631info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2010.06.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:44Zoai:ri.conicet.gov.ar:11336/248536instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:44.513CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
title Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
spellingShingle Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
Dantola, Maria Laura
Pterins
Photoinduced electron transfer
Superoxide anion
Free radicals
title_short Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
title_full Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
title_fullStr Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
title_full_unstemmed Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
title_sort Electron-transfer processes induced by the triplet state of pterins in aqueous solutions
dc.creator.none.fl_str_mv Dantola, Maria Laura
Vignoni, Mariana
González, Constanza
Lorente, Carolina
Vicendo, Patricia
Oliveros, Esther
Thomas, Andrés Héctor
author Dantola, Maria Laura
author_facet Dantola, Maria Laura
Vignoni, Mariana
González, Constanza
Lorente, Carolina
Vicendo, Patricia
Oliveros, Esther
Thomas, Andrés Héctor
author_role author
author2 Vignoni, Mariana
González, Constanza
Lorente, Carolina
Vicendo, Patricia
Oliveros, Esther
Thomas, Andrés Héctor
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Pterins
Photoinduced electron transfer
Superoxide anion
Free radicals
topic Pterins
Photoinduced electron transfer
Superoxide anion
Free radicals
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pterins (Pt) are heterocyclic compounds widespread in living systems. They participate in relevant biological processes, such as metabolic redox reactions, and can photoinduce the oxidation of biomolecules through electron transfer mechanisms. We have investigated the electron transfer pathways initiated by excited states of pterins. The study was performed with pterin (Ptr) and 6-methylpterin (Mep), selected as model compounds. The experiments were carried out in aqueous solutions under continuous UV irradiation, in the presence and in the absence of ethylenediaminetetraacetic acid (EDTA), used as an electron donor. The reactions were followed by UV/VIS spectrophotometry, HPLC and an enzymatic method for H2O2 determination. The formation of the superoxide anion (O2•‒) was investigated by electron paramagnetic resonance. The triplet excited states of Ptr and Mep are efficient electron acceptors, able to oxidize a Pt molecule in its ground state. The resulting radical anion (Pt•‒) reacts with dissolved O2 to yield O2•‒, regenerating the pterin. In the presence of EDTA, this reaction competes efficiently with the anaerobic reaction between Pt•- and EDTA•+, yielding the corresponding stable dihydroderivatives H2Pt. The effects of EDTA and dissolved O2 concentrations on the efficiencies of the different competing pathways were analyzed.
Fil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Vignoni, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: González, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Lorente, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Vicendo, Patricia. Université Paul Sabatier; Francia
Fil: Oliveros, Esther. Université Paul Sabatier; Francia
Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
description Pterins (Pt) are heterocyclic compounds widespread in living systems. They participate in relevant biological processes, such as metabolic redox reactions, and can photoinduce the oxidation of biomolecules through electron transfer mechanisms. We have investigated the electron transfer pathways initiated by excited states of pterins. The study was performed with pterin (Ptr) and 6-methylpterin (Mep), selected as model compounds. The experiments were carried out in aqueous solutions under continuous UV irradiation, in the presence and in the absence of ethylenediaminetetraacetic acid (EDTA), used as an electron donor. The reactions were followed by UV/VIS spectrophotometry, HPLC and an enzymatic method for H2O2 determination. The formation of the superoxide anion (O2•‒) was investigated by electron paramagnetic resonance. The triplet excited states of Ptr and Mep are efficient electron acceptors, able to oxidize a Pt molecule in its ground state. The resulting radical anion (Pt•‒) reacts with dissolved O2 to yield O2•‒, regenerating the pterin. In the presence of EDTA, this reaction competes efficiently with the anaerobic reaction between Pt•- and EDTA•+, yielding the corresponding stable dihydroderivatives H2Pt. The effects of EDTA and dissolved O2 concentrations on the efficiencies of the different competing pathways were analyzed.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/248536
Dantola, Maria Laura; Vignoni, Mariana; González, Constanza; Lorente, Carolina; Vicendo, Patricia; et al.; Electron-transfer processes induced by the triplet state of pterins in aqueous solutions; Elsevier Science Inc.; Free Radical Biology and Medicine; 49; 6; 9-2010; 1014-1022
0891-5849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/248536
identifier_str_mv Dantola, Maria Laura; Vignoni, Mariana; González, Constanza; Lorente, Carolina; Vicendo, Patricia; et al.; Electron-transfer processes induced by the triplet state of pterins in aqueous solutions; Elsevier Science Inc.; Free Radical Biology and Medicine; 49; 6; 9-2010; 1014-1022
0891-5849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0891584910003631
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2010.06.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc.
publisher.none.fl_str_mv Elsevier Science Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269244217098240
score 13.13397

Publicaciones similares