Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements

Autores
Masuelli, Martin Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The behavior of bovine serum albumin (BSA) in water is scarcely studied, and the thermodynamic properties arising from the experimental measurements have not been reported. Intrinsic viscosity measurements are very useful in assessing the interaction between the solute and solvent. This work discussed in a simple determination of the enthalpy of BSA in aqueous solution when the concentration ranges from 0.2 to 36.71% wt. and the temperature from 35 to 40ºC. The relationship between the concentration and intrinsic viscosity is determined according to the method of Huggins. The temperature increase reduces the ratio between inherent viscosity and concentration (/). This is reflected in the Van't Hoff curve. Furthermore, this work proposes hydrodynamic cohesion value as an indicator of the degree of affinity of protein with water and thermodynamic implications in conformational changes.
Fil: Masuelli, Martin Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Física Aplicada; Argentina. Universidad Nacional de San Luis. Departamento de Qui­mica; Argentina
Materia
Bovine Serum Albumin
Solubility
Aqueous Solutions
Intrinsic Viscosity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5698
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity MeasurementsMasuelli, Martin AlbertoBovine Serum AlbuminSolubilityAqueous SolutionsIntrinsic Viscosityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The behavior of bovine serum albumin (BSA) in water is scarcely studied, and the thermodynamic properties arising from the experimental measurements have not been reported. Intrinsic viscosity measurements are very useful in assessing the interaction between the solute and solvent. This work discussed in a simple determination of the enthalpy of BSA in aqueous solution when the concentration ranges from 0.2 to 36.71% wt. and the temperature from 35 to 40ºC. The relationship between the concentration and intrinsic viscosity is determined according to the method of Huggins. The temperature increase reduces the ratio between inherent viscosity and concentration (/). This is reflected in the Van't Hoff curve. Furthermore, this work proposes hydrodynamic cohesion value as an indicator of the degree of affinity of protein with water and thermodynamic implications in conformational changes.Fil: Masuelli, Martin Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Física Aplicada; Argentina. Universidad Nacional de San Luis. Departamento de Qui­mica; ArgentinaHindawi Publishing Corporation2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5698Masuelli, Martin Alberto; Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements; Hindawi Publishing Corporation; Advances in Physical Chemistry; 2013; 5-2013; 360239-3602391687-7993enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/360239info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/apc/2013/360239/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:19Zoai:ri.conicet.gov.ar:11336/5698instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:19.528CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
title Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
spellingShingle Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
Masuelli, Martin Alberto
Bovine Serum Albumin
Solubility
Aqueous Solutions
Intrinsic Viscosity
title_short Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
title_full Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
title_fullStr Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
title_full_unstemmed Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
title_sort Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements
dc.creator.none.fl_str_mv Masuelli, Martin Alberto
author Masuelli, Martin Alberto
author_facet Masuelli, Martin Alberto
author_role author
dc.subject.none.fl_str_mv Bovine Serum Albumin
Solubility
Aqueous Solutions
Intrinsic Viscosity
topic Bovine Serum Albumin
Solubility
Aqueous Solutions
Intrinsic Viscosity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The behavior of bovine serum albumin (BSA) in water is scarcely studied, and the thermodynamic properties arising from the experimental measurements have not been reported. Intrinsic viscosity measurements are very useful in assessing the interaction between the solute and solvent. This work discussed in a simple determination of the enthalpy of BSA in aqueous solution when the concentration ranges from 0.2 to 36.71% wt. and the temperature from 35 to 40ºC. The relationship between the concentration and intrinsic viscosity is determined according to the method of Huggins. The temperature increase reduces the ratio between inherent viscosity and concentration (/). This is reflected in the Van't Hoff curve. Furthermore, this work proposes hydrodynamic cohesion value as an indicator of the degree of affinity of protein with water and thermodynamic implications in conformational changes.
Fil: Masuelli, Martin Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Física Aplicada; Argentina. Universidad Nacional de San Luis. Departamento de Qui­mica; Argentina
description The behavior of bovine serum albumin (BSA) in water is scarcely studied, and the thermodynamic properties arising from the experimental measurements have not been reported. Intrinsic viscosity measurements are very useful in assessing the interaction between the solute and solvent. This work discussed in a simple determination of the enthalpy of BSA in aqueous solution when the concentration ranges from 0.2 to 36.71% wt. and the temperature from 35 to 40ºC. The relationship between the concentration and intrinsic viscosity is determined according to the method of Huggins. The temperature increase reduces the ratio between inherent viscosity and concentration (/). This is reflected in the Van't Hoff curve. Furthermore, this work proposes hydrodynamic cohesion value as an indicator of the degree of affinity of protein with water and thermodynamic implications in conformational changes.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5698
Masuelli, Martin Alberto; Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements; Hindawi Publishing Corporation; Advances in Physical Chemistry; 2013; 5-2013; 360239-360239
1687-7993
url http://hdl.handle.net/11336/5698
identifier_str_mv Masuelli, Martin Alberto; Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements; Hindawi Publishing Corporation; Advances in Physical Chemistry; 2013; 5-2013; 360239-360239
1687-7993
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/360239
info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/apc/2013/360239/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268967077412864
score 13.13397

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