Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption

Autores
González Vicente, Agustín; Cabral, Pablo; Hong, Nancy J.; Asirwatham, Jessica; Yang, Nianxin; Berthiaume, Jessica; Dominici, Fernando Pablo; Garvin, Jeffrey L.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fructose-enriched diets cause salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the water and salt filtered through the glomerulus. Angiotensin II (Ang II) regulates this process. Normally, dietary salt reduces Ang II allowing the kidney to excrete more salt, thereby preventing hypertension. We hypothesized that fructose-enriched diets enhance the ability of low concentrations of Ang II to stimulate PT transport. We measured the effects of a low concentration of Ang II (10−12 mol/L) on transport-related oxygen consumption (QO2), and Na/K-ATPase and Na/H-exchange (NHE) activities and expression in PTs from rats consuming tap water (Control) or 20% fructose (FRUC). In FRUC-treated PTs, Ang II increased QO2 by 14.9 ± 1.3 nmol/mg/min (p < 0.01) but had no effect in Controls. FRUC elevated NHE3 expression by 19 ± 3% (p < 0.004) but not Na/K-ATPase expression. Ang II stimulated NHE activity in FRUC PT (Δ + 0.7 ± 0.1 Arbitrary Fluorescent units (AFU)/s, p < 0.01) but not in Controls. Na/K-ATPase activity was not affected. The PKC inhibitor Gö6976 blocked the ability of FRUC to augment the actions of Ang II. FRUC did not alter the inhibitory effect of dopamine on NHE activity. We conclude that dietary fructose increases the ability of low concentrations of Ang II to stimulate PT Na reabsorption via effects on NHE.
Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cabral, Pablo. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Hong, Nancy J.. Case Western Reserve University; Estados Unidos
Fil: Asirwatham, Jessica. Case Western Reserve University; Estados Unidos
Fil: Yang, Nianxin. Case Western Reserve University; Estados Unidos
Fil: Berthiaume, Jessica. Case Western Reserve University; Estados Unidos
Fil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Garvin, Jeffrey L.. Case Western Reserve University; Estados Unidos
Materia
ANGIOTENSIN II
FRUCTOSE
SODIUM
PROXIMAL TUBULES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47359
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47359
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ ReabsorptionGonzález Vicente, AgustínCabral, PabloHong, Nancy J.Asirwatham, JessicaYang, NianxinBerthiaume, JessicaDominici, Fernando PabloGarvin, Jeffrey L.ANGIOTENSIN IIFRUCTOSESODIUMPROXIMAL TUBULEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fructose-enriched diets cause salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the water and salt filtered through the glomerulus. Angiotensin II (Ang II) regulates this process. Normally, dietary salt reduces Ang II allowing the kidney to excrete more salt, thereby preventing hypertension. We hypothesized that fructose-enriched diets enhance the ability of low concentrations of Ang II to stimulate PT transport. We measured the effects of a low concentration of Ang II (10−12 mol/L) on transport-related oxygen consumption (QO2), and Na/K-ATPase and Na/H-exchange (NHE) activities and expression in PTs from rats consuming tap water (Control) or 20% fructose (FRUC). In FRUC-treated PTs, Ang II increased QO2 by 14.9 ± 1.3 nmol/mg/min (p < 0.01) but had no effect in Controls. FRUC elevated NHE3 expression by 19 ± 3% (p < 0.004) but not Na/K-ATPase expression. Ang II stimulated NHE activity in FRUC PT (Δ + 0.7 ± 0.1 Arbitrary Fluorescent units (AFU)/s, p < 0.01) but not in Controls. Na/K-ATPase activity was not affected. The PKC inhibitor Gö6976 blocked the ability of FRUC to augment the actions of Ang II. FRUC did not alter the inhibitory effect of dopamine on NHE activity. We conclude that dietary fructose increases the ability of low concentrations of Ang II to stimulate PT Na reabsorption via effects on NHE.Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Cabral, Pablo. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Hong, Nancy J.. Case Western Reserve University; Estados UnidosFil: Asirwatham, Jessica. Case Western Reserve University; Estados UnidosFil: Yang, Nianxin. Case Western Reserve University; Estados UnidosFil: Berthiaume, Jessica. Case Western Reserve University; Estados UnidosFil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Garvin, Jeffrey L.. Case Western Reserve University; Estados UnidosMDPI2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47359González Vicente, Agustín; Cabral, Pablo; Hong, Nancy J.; Asirwatham, Jessica; Yang, Nianxin; et al.; Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption; MDPI; Nutrients; 9; 8; 8-2017; 1-152072-6643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/nu9080885info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6643/9/8/885info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:20Zoai:ri.conicet.gov.ar:11336/47359instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:21.113CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
title Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
spellingShingle Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
González Vicente, Agustín
ANGIOTENSIN II
FRUCTOSE
SODIUM
PROXIMAL TUBULES
title_short Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
title_full Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
title_fullStr Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
title_full_unstemmed Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
title_sort Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption
dc.creator.none.fl_str_mv González Vicente, Agustín
Cabral, Pablo
Hong, Nancy J.
Asirwatham, Jessica
Yang, Nianxin
Berthiaume, Jessica
Dominici, Fernando Pablo
Garvin, Jeffrey L.
author González Vicente, Agustín
author_facet González Vicente, Agustín
Cabral, Pablo
Hong, Nancy J.
Asirwatham, Jessica
Yang, Nianxin
Berthiaume, Jessica
Dominici, Fernando Pablo
Garvin, Jeffrey L.
author_role author
author2 Cabral, Pablo
Hong, Nancy J.
Asirwatham, Jessica
Yang, Nianxin
Berthiaume, Jessica
Dominici, Fernando Pablo
Garvin, Jeffrey L.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANGIOTENSIN II
FRUCTOSE
SODIUM
PROXIMAL TUBULES
topic ANGIOTENSIN II
FRUCTOSE
SODIUM
PROXIMAL TUBULES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fructose-enriched diets cause salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the water and salt filtered through the glomerulus. Angiotensin II (Ang II) regulates this process. Normally, dietary salt reduces Ang II allowing the kidney to excrete more salt, thereby preventing hypertension. We hypothesized that fructose-enriched diets enhance the ability of low concentrations of Ang II to stimulate PT transport. We measured the effects of a low concentration of Ang II (10−12 mol/L) on transport-related oxygen consumption (QO2), and Na/K-ATPase and Na/H-exchange (NHE) activities and expression in PTs from rats consuming tap water (Control) or 20% fructose (FRUC). In FRUC-treated PTs, Ang II increased QO2 by 14.9 ± 1.3 nmol/mg/min (p < 0.01) but had no effect in Controls. FRUC elevated NHE3 expression by 19 ± 3% (p < 0.004) but not Na/K-ATPase expression. Ang II stimulated NHE activity in FRUC PT (Δ + 0.7 ± 0.1 Arbitrary Fluorescent units (AFU)/s, p < 0.01) but not in Controls. Na/K-ATPase activity was not affected. The PKC inhibitor Gö6976 blocked the ability of FRUC to augment the actions of Ang II. FRUC did not alter the inhibitory effect of dopamine on NHE activity. We conclude that dietary fructose increases the ability of low concentrations of Ang II to stimulate PT Na reabsorption via effects on NHE.
Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cabral, Pablo. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Hong, Nancy J.. Case Western Reserve University; Estados Unidos
Fil: Asirwatham, Jessica. Case Western Reserve University; Estados Unidos
Fil: Yang, Nianxin. Case Western Reserve University; Estados Unidos
Fil: Berthiaume, Jessica. Case Western Reserve University; Estados Unidos
Fil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Garvin, Jeffrey L.. Case Western Reserve University; Estados Unidos
description Fructose-enriched diets cause salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the water and salt filtered through the glomerulus. Angiotensin II (Ang II) regulates this process. Normally, dietary salt reduces Ang II allowing the kidney to excrete more salt, thereby preventing hypertension. We hypothesized that fructose-enriched diets enhance the ability of low concentrations of Ang II to stimulate PT transport. We measured the effects of a low concentration of Ang II (10−12 mol/L) on transport-related oxygen consumption (QO2), and Na/K-ATPase and Na/H-exchange (NHE) activities and expression in PTs from rats consuming tap water (Control) or 20% fructose (FRUC). In FRUC-treated PTs, Ang II increased QO2 by 14.9 ± 1.3 nmol/mg/min (p < 0.01) but had no effect in Controls. FRUC elevated NHE3 expression by 19 ± 3% (p < 0.004) but not Na/K-ATPase expression. Ang II stimulated NHE activity in FRUC PT (Δ + 0.7 ± 0.1 Arbitrary Fluorescent units (AFU)/s, p < 0.01) but not in Controls. Na/K-ATPase activity was not affected. The PKC inhibitor Gö6976 blocked the ability of FRUC to augment the actions of Ang II. FRUC did not alter the inhibitory effect of dopamine on NHE activity. We conclude that dietary fructose increases the ability of low concentrations of Ang II to stimulate PT Na reabsorption via effects on NHE.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47359
González Vicente, Agustín; Cabral, Pablo; Hong, Nancy J.; Asirwatham, Jessica; Yang, Nianxin; et al.; Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption; MDPI; Nutrients; 9; 8; 8-2017; 1-15
2072-6643
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47359
identifier_str_mv González Vicente, Agustín; Cabral, Pablo; Hong, Nancy J.; Asirwatham, Jessica; Yang, Nianxin; et al.; Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption; MDPI; Nutrients; 9; 8; 8-2017; 1-15
2072-6643
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/nu9080885
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6643/9/8/885
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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