Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets

Autores
González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; Dominici, Fernando Pablo; Garvin, Jeffrey L.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.
Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Hong, Nancy J.. Case Western Reserve University; Estados Unidos
Fil: Yang, Nianxin. Case Western Reserve University; Estados Unidos
Fil: Cabral, Pablo D.. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Merk Research Laboratories; Estados Unidos. Case Western Reserve University; Estados Unidos
Fil: Berthiaume, Jessica M.. Case Western Reserve University; Estados Unidos
Fil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Garvin, Jeffrey L.. Case Western Reserve University; Estados Unidos
Materia
ANGIOTENSIN
FRUCTOSE
Na+/K+-ATPase
SODIUM EXCRETION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93978
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/93978
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt DietsGonzález Vicente, AgustínHong, Nancy J.Yang, NianxinCabral, Pablo D.Berthiaume, Jessica M.Dominici, Fernando PabloGarvin, Jeffrey L.ANGIOTENSINFRUCTOSENa+/K+-ATPaseSODIUM EXCRETIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Hong, Nancy J.. Case Western Reserve University; Estados UnidosFil: Yang, Nianxin. Case Western Reserve University; Estados UnidosFil: Cabral, Pablo D.. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Merk Research Laboratories; Estados Unidos. Case Western Reserve University; Estados UnidosFil: Berthiaume, Jessica M.. Case Western Reserve University; Estados UnidosFil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Garvin, Jeffrey L.. Case Western Reserve University; Estados UnidosMultidisciplinary Digital Publishing Institute2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93978González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; et al.; Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets; Multidisciplinary Digital Publishing Institute; Nutrients; 9-20182072-6643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6643/10/9/1244info:eu-repo/semantics/altIdentifier/doi/10.3390/nu10091244info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:50:55Zoai:ri.conicet.gov.ar:11336/93978instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:50:55.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
title Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
spellingShingle Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
González Vicente, Agustín
ANGIOTENSIN
FRUCTOSE
Na+/K+-ATPase
SODIUM EXCRETION
title_short Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
title_full Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
title_fullStr Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
title_full_unstemmed Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
title_sort Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
dc.creator.none.fl_str_mv González Vicente, Agustín
Hong, Nancy J.
Yang, Nianxin
Cabral, Pablo D.
Berthiaume, Jessica M.
Dominici, Fernando Pablo
Garvin, Jeffrey L.
author González Vicente, Agustín
author_facet González Vicente, Agustín
Hong, Nancy J.
Yang, Nianxin
Cabral, Pablo D.
Berthiaume, Jessica M.
Dominici, Fernando Pablo
Garvin, Jeffrey L.
author_role author
author2 Hong, Nancy J.
Yang, Nianxin
Cabral, Pablo D.
Berthiaume, Jessica M.
Dominici, Fernando Pablo
Garvin, Jeffrey L.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANGIOTENSIN
FRUCTOSE
Na+/K+-ATPase
SODIUM EXCRETION
topic ANGIOTENSIN
FRUCTOSE
Na+/K+-ATPase
SODIUM EXCRETION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.
Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Hong, Nancy J.. Case Western Reserve University; Estados Unidos
Fil: Yang, Nianxin. Case Western Reserve University; Estados Unidos
Fil: Cabral, Pablo D.. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Merk Research Laboratories; Estados Unidos. Case Western Reserve University; Estados Unidos
Fil: Berthiaume, Jessica M.. Case Western Reserve University; Estados Unidos
Fil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Garvin, Jeffrey L.. Case Western Reserve University; Estados Unidos
description Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93978
González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; et al.; Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets; Multidisciplinary Digital Publishing Institute; Nutrients; 9-2018
2072-6643
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93978
identifier_str_mv González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; et al.; Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets; Multidisciplinary Digital Publishing Institute; Nutrients; 9-2018
2072-6643
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6643/10/9/1244
info:eu-repo/semantics/altIdentifier/doi/10.3390/nu10091244
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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