Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets
- Autores
- González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; Dominici, Fernando Pablo; Garvin, Jeffrey L.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.
Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Hong, Nancy J.. Case Western Reserve University; Estados Unidos
Fil: Yang, Nianxin. Case Western Reserve University; Estados Unidos
Fil: Cabral, Pablo D.. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Merk Research Laboratories; Estados Unidos. Case Western Reserve University; Estados Unidos
Fil: Berthiaume, Jessica M.. Case Western Reserve University; Estados Unidos
Fil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Garvin, Jeffrey L.. Case Western Reserve University; Estados Unidos - Materia
-
ANGIOTENSIN
FRUCTOSE
Na+/K+-ATPase
SODIUM EXCRETION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/93978
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Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt DietsGonzález Vicente, AgustínHong, Nancy J.Yang, NianxinCabral, Pablo D.Berthiaume, Jessica M.Dominici, Fernando PabloGarvin, Jeffrey L.ANGIOTENSINFRUCTOSENa+/K+-ATPaseSODIUM EXCRETIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Hong, Nancy J.. Case Western Reserve University; Estados UnidosFil: Yang, Nianxin. Case Western Reserve University; Estados UnidosFil: Cabral, Pablo D.. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Merk Research Laboratories; Estados Unidos. Case Western Reserve University; Estados UnidosFil: Berthiaume, Jessica M.. Case Western Reserve University; Estados UnidosFil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Garvin, Jeffrey L.. Case Western Reserve University; Estados UnidosMultidisciplinary Digital Publishing Institute2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93978González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; et al.; Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets; Multidisciplinary Digital Publishing Institute; Nutrients; 9-20182072-6643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6643/10/9/1244info:eu-repo/semantics/altIdentifier/doi/10.3390/nu10091244info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:42:54Zoai:ri.conicet.gov.ar:11336/93978instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:42:54.528CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets |
title |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets |
spellingShingle |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets González Vicente, Agustín ANGIOTENSIN FRUCTOSE Na+/K+-ATPase SODIUM EXCRETION |
title_short |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets |
title_full |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets |
title_fullStr |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets |
title_full_unstemmed |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets |
title_sort |
Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets |
dc.creator.none.fl_str_mv |
González Vicente, Agustín Hong, Nancy J. Yang, Nianxin Cabral, Pablo D. Berthiaume, Jessica M. Dominici, Fernando Pablo Garvin, Jeffrey L. |
author |
González Vicente, Agustín |
author_facet |
González Vicente, Agustín Hong, Nancy J. Yang, Nianxin Cabral, Pablo D. Berthiaume, Jessica M. Dominici, Fernando Pablo Garvin, Jeffrey L. |
author_role |
author |
author2 |
Hong, Nancy J. Yang, Nianxin Cabral, Pablo D. Berthiaume, Jessica M. Dominici, Fernando Pablo Garvin, Jeffrey L. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
ANGIOTENSIN FRUCTOSE Na+/K+-ATPase SODIUM EXCRETION |
topic |
ANGIOTENSIN FRUCTOSE Na+/K+-ATPase SODIUM EXCRETION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension. Fil: González Vicente, Agustín. Case Western Reserve University; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Hong, Nancy J.. Case Western Reserve University; Estados Unidos Fil: Yang, Nianxin. Case Western Reserve University; Estados Unidos Fil: Cabral, Pablo D.. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Merk Research Laboratories; Estados Unidos. Case Western Reserve University; Estados Unidos Fil: Berthiaume, Jessica M.. Case Western Reserve University; Estados Unidos Fil: Dominici, Fernando Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Garvin, Jeffrey L.. Case Western Reserve University; Estados Unidos |
description |
Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/93978 González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; et al.; Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets; Multidisciplinary Digital Publishing Institute; Nutrients; 9-2018 2072-6643 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/93978 |
identifier_str_mv |
González Vicente, Agustín; Hong, Nancy J.; Yang, Nianxin; Cabral, Pablo D.; Berthiaume, Jessica M.; et al.; Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets; Multidisciplinary Digital Publishing Institute; Nutrients; 9-2018 2072-6643 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6643/10/9/1244 info:eu-repo/semantics/altIdentifier/doi/10.3390/nu10091244 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083536023453696 |
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13.22299 |