Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina

Autores
Monetti, Marina Soledad; Molina, Rosa Alejandra; Estofan, Patricia; Frutos, Maria Celia; Kiguen, Ana Ximena; Venezuela, Raul Fernando; Paglini, Maria Gabriela; Cuffini, Cecilia Gabriela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To detect and characterize Chlamydia trachomatis (C. trachomatis) genotypes in infertile patients of Córdoba, Argentina; 660 endocervical and urethral swabs and semen samples were collected from infertile patients for detection of C. trachomatis by omp A gene with Hemi Nested-PCR and cryptic plasmid-PCR. Sequencing methods of omp A gene were used to identify C. trachomatis genotypes. The sequences obtained were aligned with chlamydial sequences currently available in the GenBank, for the design of the phylogenetic tree. The prevalence of C. trachomatis was 7.27% (48/660). We did not detect C. trachomatis cryptic plasmid free strains. According to the results of nucleotide sequences, the distribution of genotypes was L1 (50 %) followed by G (25 %), E (12.5%) and D (12.5%). Patients who tested positive to genotype L1 had no symptoms of lymphogranuloma venereum (LGV). This is the first study that provides information about the distribution of C. trachomatis genotypes and the circulation of cryptic plasmid negative strains of C. trachomatis among patients with infertility in Córdoba, Argentina.
Fil: Monetti, Marina Soledad. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molina, Rosa Alejandra. Laboratorio de Andrología y Reproducción; Argentina
Fil: Estofan, Patricia. Centro Integral de Ginecología, Obstericia y Reproducción; Argentina
Fil: Frutos, Maria Celia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kiguen, Ana Ximena. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Venezuela, Raul Fernando. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Fil: Paglini, Maria Gabriela. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuffini, Cecilia Gabriela. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CLAMIDIA
GENOTYPE
INFERTILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25998
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25998
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, ArgentinaMonetti, Marina SoledadMolina, Rosa AlejandraEstofan, PatriciaFrutos, Maria CeliaKiguen, Ana XimenaVenezuela, Raul FernandoPaglini, Maria GabrielaCuffini, Cecilia GabrielaCLAMIDIAGENOTYPEINFERTILITYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3To detect and characterize Chlamydia trachomatis (C. trachomatis) genotypes in infertile patients of Córdoba, Argentina; 660 endocervical and urethral swabs and semen samples were collected from infertile patients for detection of C. trachomatis by omp A gene with Hemi Nested-PCR and cryptic plasmid-PCR. Sequencing methods of omp A gene were used to identify C. trachomatis genotypes. The sequences obtained were aligned with chlamydial sequences currently available in the GenBank, for the design of the phylogenetic tree. The prevalence of C. trachomatis was 7.27% (48/660). We did not detect C. trachomatis cryptic plasmid free strains. According to the results of nucleotide sequences, the distribution of genotypes was L1 (50 %) followed by G (25 %), E (12.5%) and D (12.5%). Patients who tested positive to genotype L1 had no symptoms of lymphogranuloma venereum (LGV). This is the first study that provides information about the distribution of C. trachomatis genotypes and the circulation of cryptic plasmid negative strains of C. trachomatis among patients with infertility in Córdoba, Argentina.Fil: Monetti, Marina Soledad. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Molina, Rosa Alejandra. Laboratorio de Andrología y Reproducción; ArgentinaFil: Estofan, Patricia. Centro Integral de Ginecología, Obstericia y Reproducción; ArgentinaFil: Frutos, Maria Celia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kiguen, Ana Ximena. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Venezuela, Raul Fernando. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; ArgentinaFil: Paglini, Maria Gabriela. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cuffini, Cecilia Gabriela. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaScientific & Academic Publishing2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25998Monetti, Marina Soledad; Molina, Rosa Alejandra; Estofan, Patricia; Frutos, Maria Celia; Kiguen, Ana Ximena; et al.; Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina; Scientific & Academic Publishing; International Journal of Virology and Molecular Biology; 2; 1; 5-2013; 1-62163-2212163-2227CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5923/j.ijvmb.20130201.01info:eu-repo/semantics/altIdentifier/url/http://article.sapub.org/10.5923.j.ijvmb.20130201.01.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:56Zoai:ri.conicet.gov.ar:11336/25998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:57.206CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
title Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
spellingShingle Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
Monetti, Marina Soledad
CLAMIDIA
GENOTYPE
INFERTILITY
title_short Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
title_full Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
title_fullStr Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
title_full_unstemmed Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
title_sort Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina
dc.creator.none.fl_str_mv Monetti, Marina Soledad
Molina, Rosa Alejandra
Estofan, Patricia
Frutos, Maria Celia
Kiguen, Ana Ximena
Venezuela, Raul Fernando
Paglini, Maria Gabriela
Cuffini, Cecilia Gabriela
author Monetti, Marina Soledad
author_facet Monetti, Marina Soledad
Molina, Rosa Alejandra
Estofan, Patricia
Frutos, Maria Celia
Kiguen, Ana Ximena
Venezuela, Raul Fernando
Paglini, Maria Gabriela
Cuffini, Cecilia Gabriela
author_role author
author2 Molina, Rosa Alejandra
Estofan, Patricia
Frutos, Maria Celia
Kiguen, Ana Ximena
Venezuela, Raul Fernando
Paglini, Maria Gabriela
Cuffini, Cecilia Gabriela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CLAMIDIA
GENOTYPE
INFERTILITY
topic CLAMIDIA
GENOTYPE
INFERTILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv To detect and characterize Chlamydia trachomatis (C. trachomatis) genotypes in infertile patients of Córdoba, Argentina; 660 endocervical and urethral swabs and semen samples were collected from infertile patients for detection of C. trachomatis by omp A gene with Hemi Nested-PCR and cryptic plasmid-PCR. Sequencing methods of omp A gene were used to identify C. trachomatis genotypes. The sequences obtained were aligned with chlamydial sequences currently available in the GenBank, for the design of the phylogenetic tree. The prevalence of C. trachomatis was 7.27% (48/660). We did not detect C. trachomatis cryptic plasmid free strains. According to the results of nucleotide sequences, the distribution of genotypes was L1 (50 %) followed by G (25 %), E (12.5%) and D (12.5%). Patients who tested positive to genotype L1 had no symptoms of lymphogranuloma venereum (LGV). This is the first study that provides information about the distribution of C. trachomatis genotypes and the circulation of cryptic plasmid negative strains of C. trachomatis among patients with infertility in Córdoba, Argentina.
Fil: Monetti, Marina Soledad. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molina, Rosa Alejandra. Laboratorio de Andrología y Reproducción; Argentina
Fil: Estofan, Patricia. Centro Integral de Ginecología, Obstericia y Reproducción; Argentina
Fil: Frutos, Maria Celia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kiguen, Ana Ximena. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Venezuela, Raul Fernando. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Fil: Paglini, Maria Gabriela. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuffini, Cecilia Gabriela. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description To detect and characterize Chlamydia trachomatis (C. trachomatis) genotypes in infertile patients of Córdoba, Argentina; 660 endocervical and urethral swabs and semen samples were collected from infertile patients for detection of C. trachomatis by omp A gene with Hemi Nested-PCR and cryptic plasmid-PCR. Sequencing methods of omp A gene were used to identify C. trachomatis genotypes. The sequences obtained were aligned with chlamydial sequences currently available in the GenBank, for the design of the phylogenetic tree. The prevalence of C. trachomatis was 7.27% (48/660). We did not detect C. trachomatis cryptic plasmid free strains. According to the results of nucleotide sequences, the distribution of genotypes was L1 (50 %) followed by G (25 %), E (12.5%) and D (12.5%). Patients who tested positive to genotype L1 had no symptoms of lymphogranuloma venereum (LGV). This is the first study that provides information about the distribution of C. trachomatis genotypes and the circulation of cryptic plasmid negative strains of C. trachomatis among patients with infertility in Córdoba, Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25998
Monetti, Marina Soledad; Molina, Rosa Alejandra; Estofan, Patricia; Frutos, Maria Celia; Kiguen, Ana Ximena; et al.; Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina; Scientific & Academic Publishing; International Journal of Virology and Molecular Biology; 2; 1; 5-2013; 1-6
2163-221
2163-2227
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25998
identifier_str_mv Monetti, Marina Soledad; Molina, Rosa Alejandra; Estofan, Patricia; Frutos, Maria Celia; Kiguen, Ana Ximena; et al.; Distribution of Chlamydia trachomatis genotypes in infertile patients of Cordoba, Argentina; Scientific & Academic Publishing; International Journal of Virology and Molecular Biology; 2; 1; 5-2013; 1-6
2163-221
2163-2227
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5923/j.ijvmb.20130201.01
info:eu-repo/semantics/altIdentifier/url/http://article.sapub.org/10.5923.j.ijvmb.20130201.01.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific & Academic Publishing
publisher.none.fl_str_mv Scientific & Academic Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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