Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility

Autores
Puerta Suarez, Jenniffer; Sanchez, Leonardo Rodolfo; Salazar, Florencia; Saka, Hector Alex; Molina, Rosa; Tissera, Andrea Daniela; Rivero, Virginia Elena; Cardona Maya, Walter D.; Motrich, Ruben Dario
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively. Highly motile human or mouse spermatozoa were in vitro exposed to C. trachomatis (serovar E or LGV) or C. muridarum, respectively. Then, sperm quality parameters were analyzed. Moreover, male fertility of Chlamydia muridarum infected male C57BL/6 mice was assessed. Human or murine sperm in vitro exposed to increasing bacterial concentrations or soluble factors from C. trachomatis or C. muridarum, respectively, did not show differences in sperm motility and viability, apoptosis, mitochondrial membrane potential, DNA fragmentation, ROS production and lipid peroxidation levels, when compared with control sperm (p > 0.05). Moreover, no differences in fertility parameters (potency, fecundity, fertility index, pre- and post-implantation loss) were observed between control and infected males. In conclusion, our results indicate that Chlamydia spp. neither directly exerts deleterious effects on spermatozoa nor impairs male fertility.
Fil: Puerta Suarez, Jenniffer. Universidad de Antioquia; Colombia
Fil: Sanchez, Leonardo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Salazar, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Molina, Rosa. Laboratorio de Andrologia y Reproduccion; Argentina
Fil: Tissera, Andrea Daniela. Laboratorio de Andrologia y Reproduccion; Argentina
Fil: Rivero, Virginia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cardona Maya, Walter D.. Universidad de Antioquia; Colombia
Fil: Motrich, Ruben Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
Chlamydia
Sperm
Infection
Male Infertility
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47518
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47518
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertilityPuerta Suarez, JennifferSanchez, Leonardo RodolfoSalazar, FlorenciaSaka, Hector AlexMolina, RosaTissera, Andrea DanielaRivero, Virginia ElenaCardona Maya, Walter D.Motrich, Ruben DarioChlamydiaSpermInfectionMale Infertilityhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively. Highly motile human or mouse spermatozoa were in vitro exposed to C. trachomatis (serovar E or LGV) or C. muridarum, respectively. Then, sperm quality parameters were analyzed. Moreover, male fertility of Chlamydia muridarum infected male C57BL/6 mice was assessed. Human or murine sperm in vitro exposed to increasing bacterial concentrations or soluble factors from C. trachomatis or C. muridarum, respectively, did not show differences in sperm motility and viability, apoptosis, mitochondrial membrane potential, DNA fragmentation, ROS production and lipid peroxidation levels, when compared with control sperm (p > 0.05). Moreover, no differences in fertility parameters (potency, fecundity, fertility index, pre- and post-implantation loss) were observed between control and infected males. In conclusion, our results indicate that Chlamydia spp. neither directly exerts deleterious effects on spermatozoa nor impairs male fertility.Fil: Puerta Suarez, Jenniffer. Universidad de Antioquia; ColombiaFil: Sanchez, Leonardo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Salazar, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Molina, Rosa. Laboratorio de Andrologia y Reproduccion; ArgentinaFil: Tissera, Andrea Daniela. Laboratorio de Andrologia y Reproduccion; ArgentinaFil: Rivero, Virginia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cardona Maya, Walter D.. Universidad de Antioquia; ColombiaFil: Motrich, Ruben Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaNature Publishing Group2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47518Puerta Suarez, Jenniffer; Sanchez, Leonardo Rodolfo; Salazar, Florencia; Saka, Hector Alex; Molina, Rosa; et al.; Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility; Nature Publishing Group; Scientific Reports; 7; 1; 4-20172045-23222045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-017-01262-winfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-01262-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:29Zoai:ri.conicet.gov.ar:11336/47518instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:30.143CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
title Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
spellingShingle Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
Puerta Suarez, Jenniffer
Chlamydia
Sperm
Infection
Male Infertility
title_short Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
title_full Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
title_fullStr Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
title_full_unstemmed Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
title_sort Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
dc.creator.none.fl_str_mv Puerta Suarez, Jenniffer
Sanchez, Leonardo Rodolfo
Salazar, Florencia
Saka, Hector Alex
Molina, Rosa
Tissera, Andrea Daniela
Rivero, Virginia Elena
Cardona Maya, Walter D.
Motrich, Ruben Dario
author Puerta Suarez, Jenniffer
author_facet Puerta Suarez, Jenniffer
Sanchez, Leonardo Rodolfo
Salazar, Florencia
Saka, Hector Alex
Molina, Rosa
Tissera, Andrea Daniela
Rivero, Virginia Elena
Cardona Maya, Walter D.
Motrich, Ruben Dario
author_role author
author2 Sanchez, Leonardo Rodolfo
Salazar, Florencia
Saka, Hector Alex
Molina, Rosa
Tissera, Andrea Daniela
Rivero, Virginia Elena
Cardona Maya, Walter D.
Motrich, Ruben Dario
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chlamydia
Sperm
Infection
Male Infertility
topic Chlamydia
Sperm
Infection
Male Infertility
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively. Highly motile human or mouse spermatozoa were in vitro exposed to C. trachomatis (serovar E or LGV) or C. muridarum, respectively. Then, sperm quality parameters were analyzed. Moreover, male fertility of Chlamydia muridarum infected male C57BL/6 mice was assessed. Human or murine sperm in vitro exposed to increasing bacterial concentrations or soluble factors from C. trachomatis or C. muridarum, respectively, did not show differences in sperm motility and viability, apoptosis, mitochondrial membrane potential, DNA fragmentation, ROS production and lipid peroxidation levels, when compared with control sperm (p > 0.05). Moreover, no differences in fertility parameters (potency, fecundity, fertility index, pre- and post-implantation loss) were observed between control and infected males. In conclusion, our results indicate that Chlamydia spp. neither directly exerts deleterious effects on spermatozoa nor impairs male fertility.
Fil: Puerta Suarez, Jenniffer. Universidad de Antioquia; Colombia
Fil: Sanchez, Leonardo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Salazar, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Molina, Rosa. Laboratorio de Andrologia y Reproduccion; Argentina
Fil: Tissera, Andrea Daniela. Laboratorio de Andrologia y Reproduccion; Argentina
Fil: Rivero, Virginia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cardona Maya, Walter D.. Universidad de Antioquia; Colombia
Fil: Motrich, Ruben Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively. Highly motile human or mouse spermatozoa were in vitro exposed to C. trachomatis (serovar E or LGV) or C. muridarum, respectively. Then, sperm quality parameters were analyzed. Moreover, male fertility of Chlamydia muridarum infected male C57BL/6 mice was assessed. Human or murine sperm in vitro exposed to increasing bacterial concentrations or soluble factors from C. trachomatis or C. muridarum, respectively, did not show differences in sperm motility and viability, apoptosis, mitochondrial membrane potential, DNA fragmentation, ROS production and lipid peroxidation levels, when compared with control sperm (p > 0.05). Moreover, no differences in fertility parameters (potency, fecundity, fertility index, pre- and post-implantation loss) were observed between control and infected males. In conclusion, our results indicate that Chlamydia spp. neither directly exerts deleterious effects on spermatozoa nor impairs male fertility.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47518
Puerta Suarez, Jenniffer; Sanchez, Leonardo Rodolfo; Salazar, Florencia; Saka, Hector Alex; Molina, Rosa; et al.; Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility; Nature Publishing Group; Scientific Reports; 7; 1; 4-2017
2045-2322
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47518
identifier_str_mv Puerta Suarez, Jenniffer; Sanchez, Leonardo Rodolfo; Salazar, Florencia; Saka, Hector Alex; Molina, Rosa; et al.; Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility; Nature Publishing Group; Scientific Reports; 7; 1; 4-2017
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-017-01262-w
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-01262-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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