Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
- Autores
- Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; Vay, Carlos Alberto; Barrios, Rubén; Gutkind, Gabriel Osvaldo; Di Conza, José Alejandro
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.
Fil: Figueroa Espinosa, Roque Arnulfo. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rumi, María Valeria. Universidad de Buenos Aires; Argentina
Fil: Marchisio, Martin Luis. Universidad Nacional del Litoral; Argentina
Fil: Cejas, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires; Argentina
Fil: Barrios, Rubén. Bd Life Sciences; Argentina
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
CMY-2
ESCHERICHIA COLI
MALDI-TOF
Β-LACTAMASE DETECTION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/114229
Ver los metadatos del registro completo
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spelling |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometryFigueroa Espinosa, Roque ArnulfoRumi, María ValeriaMarchisio, Martin LuisCejas, DanielaRadice, Marcela AlejandraVay, Carlos AlbertoBarrios, RubénGutkind, Gabriel OsvaldoDi Conza, José AlejandroCMY-2ESCHERICHIA COLIMALDI-TOFΒ-LACTAMASE DETECTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.Fil: Figueroa Espinosa, Roque Arnulfo. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rumi, María Valeria. Universidad de Buenos Aires; ArgentinaFil: Marchisio, Martin Luis. Universidad Nacional del Litoral; ArgentinaFil: Cejas, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires; ArgentinaFil: Barrios, Rubén. Bd Life Sciences; ArgentinaFil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Di Conza, José Alejandro. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114229Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-280167-7012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2018.04.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167701218300393info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:38Zoai:ri.conicet.gov.ar:11336/114229instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:38.301CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry |
title |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry |
spellingShingle |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry Figueroa Espinosa, Roque Arnulfo CMY-2 ESCHERICHIA COLI MALDI-TOF Β-LACTAMASE DETECTION |
title_short |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry |
title_full |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry |
title_fullStr |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry |
title_full_unstemmed |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry |
title_sort |
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry |
dc.creator.none.fl_str_mv |
Figueroa Espinosa, Roque Arnulfo Rumi, María Valeria Marchisio, Martin Luis Cejas, Daniela Radice, Marcela Alejandra Vay, Carlos Alberto Barrios, Rubén Gutkind, Gabriel Osvaldo Di Conza, José Alejandro |
author |
Figueroa Espinosa, Roque Arnulfo |
author_facet |
Figueroa Espinosa, Roque Arnulfo Rumi, María Valeria Marchisio, Martin Luis Cejas, Daniela Radice, Marcela Alejandra Vay, Carlos Alberto Barrios, Rubén Gutkind, Gabriel Osvaldo Di Conza, José Alejandro |
author_role |
author |
author2 |
Rumi, María Valeria Marchisio, Martin Luis Cejas, Daniela Radice, Marcela Alejandra Vay, Carlos Alberto Barrios, Rubén Gutkind, Gabriel Osvaldo Di Conza, José Alejandro |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
CMY-2 ESCHERICHIA COLI MALDI-TOF Β-LACTAMASE DETECTION |
topic |
CMY-2 ESCHERICHIA COLI MALDI-TOF Β-LACTAMASE DETECTION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers. Fil: Figueroa Espinosa, Roque Arnulfo. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rumi, María Valeria. Universidad de Buenos Aires; Argentina Fil: Marchisio, Martin Luis. Universidad Nacional del Litoral; Argentina Fil: Cejas, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina Fil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires; Argentina Fil: Barrios, Rubén. Bd Life Sciences; Argentina Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina Fil: Di Conza, José Alejandro. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/114229 Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-28 0167-7012 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/114229 |
identifier_str_mv |
Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-28 0167-7012 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2018.04.001 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167701218300393 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |