Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry

Autores
Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; Vay, Carlos Alberto; Barrios, Rubén; Gutkind, Gabriel Osvaldo; Di Conza, José Alejandro
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.
Fil: Figueroa Espinosa, Roque Arnulfo. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rumi, María Valeria. Universidad de Buenos Aires; Argentina
Fil: Marchisio, Martin Luis. Universidad Nacional del Litoral; Argentina
Fil: Cejas, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires; Argentina
Fil: Barrios, Rubén. Bd Life Sciences; Argentina
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CMY-2
ESCHERICHIA COLI
MALDI-TOF
Β-LACTAMASE DETECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114229

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oai_identifier_str oai:ri.conicet.gov.ar:11336/114229
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometryFigueroa Espinosa, Roque ArnulfoRumi, María ValeriaMarchisio, Martin LuisCejas, DanielaRadice, Marcela AlejandraVay, Carlos AlbertoBarrios, RubénGutkind, Gabriel OsvaldoDi Conza, José AlejandroCMY-2ESCHERICHIA COLIMALDI-TOFΒ-LACTAMASE DETECTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.Fil: Figueroa Espinosa, Roque Arnulfo. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rumi, María Valeria. Universidad de Buenos Aires; ArgentinaFil: Marchisio, Martin Luis. Universidad Nacional del Litoral; ArgentinaFil: Cejas, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires; ArgentinaFil: Barrios, Rubén. Bd Life Sciences; ArgentinaFil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Di Conza, José Alejandro. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114229Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-280167-7012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2018.04.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167701218300393info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:38Zoai:ri.conicet.gov.ar:11336/114229instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:38.301CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
title Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
spellingShingle Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
Figueroa Espinosa, Roque Arnulfo
CMY-2
ESCHERICHIA COLI
MALDI-TOF
Β-LACTAMASE DETECTION
title_short Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
title_full Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
title_fullStr Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
title_full_unstemmed Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
title_sort Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
dc.creator.none.fl_str_mv Figueroa Espinosa, Roque Arnulfo
Rumi, María Valeria
Marchisio, Martin Luis
Cejas, Daniela
Radice, Marcela Alejandra
Vay, Carlos Alberto
Barrios, Rubén
Gutkind, Gabriel Osvaldo
Di Conza, José Alejandro
author Figueroa Espinosa, Roque Arnulfo
author_facet Figueroa Espinosa, Roque Arnulfo
Rumi, María Valeria
Marchisio, Martin Luis
Cejas, Daniela
Radice, Marcela Alejandra
Vay, Carlos Alberto
Barrios, Rubén
Gutkind, Gabriel Osvaldo
Di Conza, José Alejandro
author_role author
author2 Rumi, María Valeria
Marchisio, Martin Luis
Cejas, Daniela
Radice, Marcela Alejandra
Vay, Carlos Alberto
Barrios, Rubén
Gutkind, Gabriel Osvaldo
Di Conza, José Alejandro
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CMY-2
ESCHERICHIA COLI
MALDI-TOF
Β-LACTAMASE DETECTION
topic CMY-2
ESCHERICHIA COLI
MALDI-TOF
Β-LACTAMASE DETECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.
Fil: Figueroa Espinosa, Roque Arnulfo. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rumi, María Valeria. Universidad de Buenos Aires; Argentina
Fil: Marchisio, Martin Luis. Universidad Nacional del Litoral; Argentina
Fil: Cejas, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires; Argentina
Fil: Barrios, Rubén. Bd Life Sciences; Argentina
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114229
Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-28
0167-7012
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114229
identifier_str_mv Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-28
0167-7012
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2018.04.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167701218300393
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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