In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces

Autores
Fernandez Juri, Maria Guillermina; Dalcero, Ana Maria; Magnoli, Carina Elizabeth
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: This study evaluated the binding capacity of aflatoxin B1 (AFB1) by two Enterococcus faecium strains (MF4 and GJ40) isolated from faeces from healthy dogs. Materials and Methods: The binding assay was performed using 50 and 100 ppb of AFB1 analysing the effects of the viability, incubation time and pH on AFB1 binding. Binding stability was determined by washing three times the bacteria-AFB1 complexes with phosphate buffer saline. Results: Both GJ40 and MF4 strains have the ability to remove AFB1 from aqueous solution. Viable cells were slightly more effective in AFB1 binding than nonviable ones for both strains. Enterococcus faeciumGJ40 removes 24–27% and 17–24%, and Ent. faeciumMF4 removes 36–42% and 27–32% of AFB1 (50 and 100 ppb, respectively) throughout a 48 h incubation period. In general, the removal of AFB1 was highest at pH 7·00 for both strains. The stability of the bacteria-AFB1 complex formed was found to be high (up to 50% of AFB1 remained bounded in bacterial cell after three washes with phosphate buffered saline). Conclusion: The Ent. faecium strains assayed are capable of removing AFB1 under different conditions in vitro. Significance and Impact of the Study: This is the first AFB1 binding assay performed with Ent. faecium strains isolated from dog faeces, being an interesting strategy for AFB1 decontamination of pet food.
Fil: Fernandez Juri, Maria Guillermina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Magnoli, Carina Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Aflatoxin B1
Binding Ability
Enterococcus Faecium
Food Safety
Healthy Dogs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34415
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/34415
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faecesFernandez Juri, Maria GuillerminaDalcero, Ana MariaMagnoli, Carina ElizabethAflatoxin B1Binding AbilityEnterococcus FaeciumFood SafetyHealthy Dogshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aim: This study evaluated the binding capacity of aflatoxin B1 (AFB1) by two Enterococcus faecium strains (MF4 and GJ40) isolated from faeces from healthy dogs. Materials and Methods: The binding assay was performed using 50 and 100 ppb of AFB1 analysing the effects of the viability, incubation time and pH on AFB1 binding. Binding stability was determined by washing three times the bacteria-AFB1 complexes with phosphate buffer saline. Results: Both GJ40 and MF4 strains have the ability to remove AFB1 from aqueous solution. Viable cells were slightly more effective in AFB1 binding than nonviable ones for both strains. Enterococcus faeciumGJ40 removes 24–27% and 17–24%, and Ent. faeciumMF4 removes 36–42% and 27–32% of AFB1 (50 and 100 ppb, respectively) throughout a 48 h incubation period. In general, the removal of AFB1 was highest at pH 7·00 for both strains. The stability of the bacteria-AFB1 complex formed was found to be high (up to 50% of AFB1 remained bounded in bacterial cell after three washes with phosphate buffered saline). Conclusion: The Ent. faecium strains assayed are capable of removing AFB1 under different conditions in vitro. Significance and Impact of the Study: This is the first AFB1 binding assay performed with Ent. faecium strains isolated from dog faeces, being an interesting strategy for AFB1 decontamination of pet food.Fil: Fernandez Juri, Maria Guillermina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Magnoli, Carina Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34415Fernandez Juri, Maria Guillermina; Dalcero, Ana Maria; Magnoli, Carina Elizabeth; In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces; Wiley; Journal of Applied Microbiology; 118; 3; 1-2015; 574-5821364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12726info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12726/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:14Zoai:ri.conicet.gov.ar:11336/34415instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:14.69CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
title In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
spellingShingle In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
Fernandez Juri, Maria Guillermina
Aflatoxin B1
Binding Ability
Enterococcus Faecium
Food Safety
Healthy Dogs
title_short In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
title_full In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
title_fullStr In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
title_full_unstemmed In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
title_sort In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces
dc.creator.none.fl_str_mv Fernandez Juri, Maria Guillermina
Dalcero, Ana Maria
Magnoli, Carina Elizabeth
author Fernandez Juri, Maria Guillermina
author_facet Fernandez Juri, Maria Guillermina
Dalcero, Ana Maria
Magnoli, Carina Elizabeth
author_role author
author2 Dalcero, Ana Maria
Magnoli, Carina Elizabeth
author2_role author
author
dc.subject.none.fl_str_mv Aflatoxin B1
Binding Ability
Enterococcus Faecium
Food Safety
Healthy Dogs
topic Aflatoxin B1
Binding Ability
Enterococcus Faecium
Food Safety
Healthy Dogs
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aim: This study evaluated the binding capacity of aflatoxin B1 (AFB1) by two Enterococcus faecium strains (MF4 and GJ40) isolated from faeces from healthy dogs. Materials and Methods: The binding assay was performed using 50 and 100 ppb of AFB1 analysing the effects of the viability, incubation time and pH on AFB1 binding. Binding stability was determined by washing three times the bacteria-AFB1 complexes with phosphate buffer saline. Results: Both GJ40 and MF4 strains have the ability to remove AFB1 from aqueous solution. Viable cells were slightly more effective in AFB1 binding than nonviable ones for both strains. Enterococcus faeciumGJ40 removes 24–27% and 17–24%, and Ent. faeciumMF4 removes 36–42% and 27–32% of AFB1 (50 and 100 ppb, respectively) throughout a 48 h incubation period. In general, the removal of AFB1 was highest at pH 7·00 for both strains. The stability of the bacteria-AFB1 complex formed was found to be high (up to 50% of AFB1 remained bounded in bacterial cell after three washes with phosphate buffered saline). Conclusion: The Ent. faecium strains assayed are capable of removing AFB1 under different conditions in vitro. Significance and Impact of the Study: This is the first AFB1 binding assay performed with Ent. faecium strains isolated from dog faeces, being an interesting strategy for AFB1 decontamination of pet food.
Fil: Fernandez Juri, Maria Guillermina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Magnoli, Carina Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aim: This study evaluated the binding capacity of aflatoxin B1 (AFB1) by two Enterococcus faecium strains (MF4 and GJ40) isolated from faeces from healthy dogs. Materials and Methods: The binding assay was performed using 50 and 100 ppb of AFB1 analysing the effects of the viability, incubation time and pH on AFB1 binding. Binding stability was determined by washing three times the bacteria-AFB1 complexes with phosphate buffer saline. Results: Both GJ40 and MF4 strains have the ability to remove AFB1 from aqueous solution. Viable cells were slightly more effective in AFB1 binding than nonviable ones for both strains. Enterococcus faeciumGJ40 removes 24–27% and 17–24%, and Ent. faeciumMF4 removes 36–42% and 27–32% of AFB1 (50 and 100 ppb, respectively) throughout a 48 h incubation period. In general, the removal of AFB1 was highest at pH 7·00 for both strains. The stability of the bacteria-AFB1 complex formed was found to be high (up to 50% of AFB1 remained bounded in bacterial cell after three washes with phosphate buffered saline). Conclusion: The Ent. faecium strains assayed are capable of removing AFB1 under different conditions in vitro. Significance and Impact of the Study: This is the first AFB1 binding assay performed with Ent. faecium strains isolated from dog faeces, being an interesting strategy for AFB1 decontamination of pet food.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34415
Fernandez Juri, Maria Guillermina; Dalcero, Ana Maria; Magnoli, Carina Elizabeth; In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces; Wiley; Journal of Applied Microbiology; 118; 3; 1-2015; 574-582
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34415
identifier_str_mv Fernandez Juri, Maria Guillermina; Dalcero, Ana Maria; Magnoli, Carina Elizabeth; In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces; Wiley; Journal of Applied Microbiology; 118; 3; 1-2015; 574-582
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12726
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12726/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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