Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection

Autores
Piloni, Natacha Estefanía; Perazzo, Juan C.; Fernandez, Virginia; Videla, Luis A.; Puntarulo, Susana Ángela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work was aimed to test the hypothesis that sub-chronic administration of iron-dextran (Fe-dextran) (six doses of 50 mg Fe-dextran/kg) to rats triggers a transient oxidative stress in brain and mechanisms of cellular antioxidant defence. After 2 h of administration of the 6th dose, a significant increase of total Fe, the labile Fe pool (LIP), the lipid radical (LR•)/α-tocopherol (α-T) content ratio were observed, as compared to values in control brain homogenates. The ascorbyl radical (A•)/ascorbate (AH-) content ratio and the oxidation rate of 2′,7′-dichlorodihidrofluorescein (DCFH-DA) were significantly higher in Fe-dextran treated rats, as compared to values in brain from control rats after 4 h treatment. An increase in both catalase (CAT) and superoxide dismutase (SOD) activity was observed at 8 and 1-2 h, respectively. No significant changes were detected in the nuclear factor-κB (NF-κB) levels in nuclear extracts from rat brains after 1-8 h of Fe-dextran administration. After 2 h of Fe administration Fe concentration in cortex, striatum and hippocampus was significantly increased as compared to the same areas from control animals. Both, CAT and SOD activities were significantly increased in cortex after Fe administration over control values, without changes in striatum and hippocampus. Taken as a whole, sub-chronic Fe administration enhances the steady state concentration of Fe in the brain LIP that favors the settlement of an initial oxidative stress condition, both at hydrophilic and lipophilic compartments, resulting in cellular protection evidenced by antioxidant enzyme upregulation.
Fil: Piloni, Natacha Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Perazzo, Juan C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Fernandez, Virginia. Universidad de Chile; Chile
Fil: Videla, Luis A.. Universidad de Chile; Chile
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
Brain
Catalase
Iron
Nf-Κb
Oxidative Stress
Superoxide Dismutase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38848
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38848
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protectionPiloni, Natacha EstefaníaPerazzo, Juan C.Fernandez, VirginiaVidela, Luis A.Puntarulo, Susana ÁngelaBrainCatalaseIronNf-ΚbOxidative StressSuperoxide Dismutasehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3This work was aimed to test the hypothesis that sub-chronic administration of iron-dextran (Fe-dextran) (six doses of 50 mg Fe-dextran/kg) to rats triggers a transient oxidative stress in brain and mechanisms of cellular antioxidant defence. After 2 h of administration of the 6th dose, a significant increase of total Fe, the labile Fe pool (LIP), the lipid radical (LR•)/α-tocopherol (α-T) content ratio were observed, as compared to values in control brain homogenates. The ascorbyl radical (A•)/ascorbate (AH-) content ratio and the oxidation rate of 2′,7′-dichlorodihidrofluorescein (DCFH-DA) were significantly higher in Fe-dextran treated rats, as compared to values in brain from control rats after 4 h treatment. An increase in both catalase (CAT) and superoxide dismutase (SOD) activity was observed at 8 and 1-2 h, respectively. No significant changes were detected in the nuclear factor-κB (NF-κB) levels in nuclear extracts from rat brains after 1-8 h of Fe-dextran administration. After 2 h of Fe administration Fe concentration in cortex, striatum and hippocampus was significantly increased as compared to the same areas from control animals. Both, CAT and SOD activities were significantly increased in cortex after Fe administration over control values, without changes in striatum and hippocampus. Taken as a whole, sub-chronic Fe administration enhances the steady state concentration of Fe in the brain LIP that favors the settlement of an initial oxidative stress condition, both at hydrophilic and lipophilic compartments, resulting in cellular protection evidenced by antioxidant enzyme upregulation.Fil: Piloni, Natacha Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Perazzo, Juan C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Fernandez, Virginia. Universidad de Chile; ChileFil: Videla, Luis A.. Universidad de Chile; ChileFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaSpringer2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38848Piloni, Natacha Estefanía; Perazzo, Juan C.; Fernandez, Virginia; Videla, Luis A.; Puntarulo, Susana Ángela; Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection; Springer; Biometals; 29; 1; 2-2016; 119-1300966-08441572-8773CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10534-015-9902-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10534-015-9902-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:45Zoai:ri.conicet.gov.ar:11336/38848instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:45.546CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
title Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
spellingShingle Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
Piloni, Natacha Estefanía
Brain
Catalase
Iron
Nf-Κb
Oxidative Stress
Superoxide Dismutase
title_short Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
title_full Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
title_fullStr Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
title_full_unstemmed Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
title_sort Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection
dc.creator.none.fl_str_mv Piloni, Natacha Estefanía
Perazzo, Juan C.
Fernandez, Virginia
Videla, Luis A.
Puntarulo, Susana Ángela
author Piloni, Natacha Estefanía
author_facet Piloni, Natacha Estefanía
Perazzo, Juan C.
Fernandez, Virginia
Videla, Luis A.
Puntarulo, Susana Ángela
author_role author
author2 Perazzo, Juan C.
Fernandez, Virginia
Videla, Luis A.
Puntarulo, Susana Ángela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Brain
Catalase
Iron
Nf-Κb
Oxidative Stress
Superoxide Dismutase
topic Brain
Catalase
Iron
Nf-Κb
Oxidative Stress
Superoxide Dismutase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This work was aimed to test the hypothesis that sub-chronic administration of iron-dextran (Fe-dextran) (six doses of 50 mg Fe-dextran/kg) to rats triggers a transient oxidative stress in brain and mechanisms of cellular antioxidant defence. After 2 h of administration of the 6th dose, a significant increase of total Fe, the labile Fe pool (LIP), the lipid radical (LR•)/α-tocopherol (α-T) content ratio were observed, as compared to values in control brain homogenates. The ascorbyl radical (A•)/ascorbate (AH-) content ratio and the oxidation rate of 2′,7′-dichlorodihidrofluorescein (DCFH-DA) were significantly higher in Fe-dextran treated rats, as compared to values in brain from control rats after 4 h treatment. An increase in both catalase (CAT) and superoxide dismutase (SOD) activity was observed at 8 and 1-2 h, respectively. No significant changes were detected in the nuclear factor-κB (NF-κB) levels in nuclear extracts from rat brains after 1-8 h of Fe-dextran administration. After 2 h of Fe administration Fe concentration in cortex, striatum and hippocampus was significantly increased as compared to the same areas from control animals. Both, CAT and SOD activities were significantly increased in cortex after Fe administration over control values, without changes in striatum and hippocampus. Taken as a whole, sub-chronic Fe administration enhances the steady state concentration of Fe in the brain LIP that favors the settlement of an initial oxidative stress condition, both at hydrophilic and lipophilic compartments, resulting in cellular protection evidenced by antioxidant enzyme upregulation.
Fil: Piloni, Natacha Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Perazzo, Juan C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Fernandez, Virginia. Universidad de Chile; Chile
Fil: Videla, Luis A.. Universidad de Chile; Chile
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
description This work was aimed to test the hypothesis that sub-chronic administration of iron-dextran (Fe-dextran) (six doses of 50 mg Fe-dextran/kg) to rats triggers a transient oxidative stress in brain and mechanisms of cellular antioxidant defence. After 2 h of administration of the 6th dose, a significant increase of total Fe, the labile Fe pool (LIP), the lipid radical (LR•)/α-tocopherol (α-T) content ratio were observed, as compared to values in control brain homogenates. The ascorbyl radical (A•)/ascorbate (AH-) content ratio and the oxidation rate of 2′,7′-dichlorodihidrofluorescein (DCFH-DA) were significantly higher in Fe-dextran treated rats, as compared to values in brain from control rats after 4 h treatment. An increase in both catalase (CAT) and superoxide dismutase (SOD) activity was observed at 8 and 1-2 h, respectively. No significant changes were detected in the nuclear factor-κB (NF-κB) levels in nuclear extracts from rat brains after 1-8 h of Fe-dextran administration. After 2 h of Fe administration Fe concentration in cortex, striatum and hippocampus was significantly increased as compared to the same areas from control animals. Both, CAT and SOD activities were significantly increased in cortex after Fe administration over control values, without changes in striatum and hippocampus. Taken as a whole, sub-chronic Fe administration enhances the steady state concentration of Fe in the brain LIP that favors the settlement of an initial oxidative stress condition, both at hydrophilic and lipophilic compartments, resulting in cellular protection evidenced by antioxidant enzyme upregulation.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38848
Piloni, Natacha Estefanía; Perazzo, Juan C.; Fernandez, Virginia; Videla, Luis A.; Puntarulo, Susana Ángela; Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection; Springer; Biometals; 29; 1; 2-2016; 119-130
0966-0844
1572-8773
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38848
identifier_str_mv Piloni, Natacha Estefanía; Perazzo, Juan C.; Fernandez, Virginia; Videla, Luis A.; Puntarulo, Susana Ángela; Sub-chronic iron overload triggers oxidative stress development in rat brain: Implications for cell protection; Springer; Biometals; 29; 1; 2-2016; 119-130
0966-0844
1572-8773
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10534-015-9902-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10534-015-9902-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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