Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation

Autores
Pomares, Maria Fernanda; Salomon, Raul Armando; Pavlova, Olga; Severinov, Konstantin; Farias, Ricardo Norberto; Vincent, Paula Andrea
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli, including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative. In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing its potential use in food preservation. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salomon, Raul Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pavlova, Olga. State University of New Jersey; Estados Unidos
Fil: Severinov, Konstantin. State University of New Jersey; Estados Unidos. Russian Academy of Science; Rusia
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Microcin J25
Chymotrypsin-Susceptible
Food Preservation
Potential Applicability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65852
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/65852
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservationPomares, Maria FernandaSalomon, Raul ArmandoPavlova, OlgaSeverinov, KonstantinFarias, Ricardo NorbertoVincent, Paula AndreaMicrocin J25Chymotrypsin-SusceptibleFood PreservationPotential Applicabilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli, including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative. In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing its potential use in food preservation. Copyright © 2009, American Society for Microbiology. All Rights Reserved.Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Salomon, Raul Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pavlova, Olga. State University of New Jersey; Estados UnidosFil: Severinov, Konstantin. State University of New Jersey; Estados Unidos. Russian Academy of Science; RusiaFil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaAmerican Society for Microbiology2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65852Pomares, Maria Fernanda; Salomon, Raul Armando; Pavlova, Olga; Severinov, Konstantin; Farias, Ricardo Norberto; et al.; Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation; American Society for Microbiology; Applied And Environmental Microbiology; 75; 17; 9-2009; 5734-57380099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/75/17/5734info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.01070-09info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:38Zoai:ri.conicet.gov.ar:11336/65852instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:38.733CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
title Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
spellingShingle Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
Pomares, Maria Fernanda
Microcin J25
Chymotrypsin-Susceptible
Food Preservation
Potential Applicability
title_short Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
title_full Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
title_fullStr Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
title_full_unstemmed Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
title_sort Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
dc.creator.none.fl_str_mv Pomares, Maria Fernanda
Salomon, Raul Armando
Pavlova, Olga
Severinov, Konstantin
Farias, Ricardo Norberto
Vincent, Paula Andrea
author Pomares, Maria Fernanda
author_facet Pomares, Maria Fernanda
Salomon, Raul Armando
Pavlova, Olga
Severinov, Konstantin
Farias, Ricardo Norberto
Vincent, Paula Andrea
author_role author
author2 Salomon, Raul Armando
Pavlova, Olga
Severinov, Konstantin
Farias, Ricardo Norberto
Vincent, Paula Andrea
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Microcin J25
Chymotrypsin-Susceptible
Food Preservation
Potential Applicability
topic Microcin J25
Chymotrypsin-Susceptible
Food Preservation
Potential Applicability
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli, including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative. In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing its potential use in food preservation. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salomon, Raul Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pavlova, Olga. State University of New Jersey; Estados Unidos
Fil: Severinov, Konstantin. State University of New Jersey; Estados Unidos. Russian Academy of Science; Rusia
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli, including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative. In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing its potential use in food preservation. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65852
Pomares, Maria Fernanda; Salomon, Raul Armando; Pavlova, Olga; Severinov, Konstantin; Farias, Ricardo Norberto; et al.; Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation; American Society for Microbiology; Applied And Environmental Microbiology; 75; 17; 9-2009; 5734-5738
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65852
identifier_str_mv Pomares, Maria Fernanda; Salomon, Raul Armando; Pavlova, Olga; Severinov, Konstantin; Farias, Ricardo Norberto; et al.; Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation; American Society for Microbiology; Applied And Environmental Microbiology; 75; 17; 9-2009; 5734-5738
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/75/17/5734
info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.01070-09
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613404419948544
score 13.070432

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