Endophytes from wheat as biocontrol agents against tan spot disease

Autores
Larran, Silvina; Simon, Maria Rosa; Moreno, Maria Virginia; Santamarina Siurana, M. P.; Perello, Analia Edith
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against Pyrenophora tritici-repentis (Died.) Drechsler (anamorph Drechslera tritici-repentis) (Died.) Shoem (Dtr), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced Dtr growth compared to the control except Rhodotorula rubra from 11 to 15 days post inoculation. Trichoderma hamatum, Penicillium sp., Bacillus sp. and Paecilomyces lilacinus significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of D. tritici-repentis. In addition, two endophytes, Bacillus sp. and Fusarium sp., reduced significantly the percent spore germination of Dtr compared to the control by 82% and 52% respectively. In greenhouse experiment T. hamatum, Chaetomium globosum and Fusarium sp. significantly (P ⩽ 0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with T. hamatum as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise, Bacillus sp. was other highlighted microorganism that antagonized the pathogen in in vitro assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by D. tritici-repentis, particularly T. hamatum and Bacillus sp.
Fil: Larran, Silvina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Simon, Maria Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Moreno, Maria Virginia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Santamarina Siurana, M. P.. Universidad Politécnica de Valencia; España
Fil: Perello, Analia Edith. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Endophytes
Drechslera Tritici Repentis
Pyrenophora Tritici Repentis
Wheat
Endophytic Fungi
Tan Spot
Triticum Aestivum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27694
Acceder
id CONICETDig_2c1762a358a17e2cb4394bab57857c10
oai_identifier_str oai:ri.conicet.gov.ar:11336/27694
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endophytes from wheat as biocontrol agents against tan spot diseaseLarran, SilvinaSimon, Maria RosaMoreno, Maria VirginiaSantamarina Siurana, M. P.Perello, Analia EdithEndophytesDrechslera Tritici RepentisPyrenophora Tritici RepentisWheatEndophytic FungiTan SpotTriticum Aestivumhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against Pyrenophora tritici-repentis (Died.) Drechsler (anamorph Drechslera tritici-repentis) (Died.) Shoem (Dtr), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced Dtr growth compared to the control except Rhodotorula rubra from 11 to 15 days post inoculation. Trichoderma hamatum, Penicillium sp., Bacillus sp. and Paecilomyces lilacinus significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of D. tritici-repentis. In addition, two endophytes, Bacillus sp. and Fusarium sp., reduced significantly the percent spore germination of Dtr compared to the control by 82% and 52% respectively. In greenhouse experiment T. hamatum, Chaetomium globosum and Fusarium sp. significantly (P ⩽ 0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with T. hamatum as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise, Bacillus sp. was other highlighted microorganism that antagonized the pathogen in in vitro assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by D. tritici-repentis, particularly T. hamatum and Bacillus sp.Fil: Larran, Silvina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; ArgentinaFil: Simon, Maria Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; ArgentinaFil: Moreno, Maria Virginia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Santamarina Siurana, M. P.. Universidad Politécnica de Valencia; EspañaFil: Perello, Analia Edith. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27694Larran, Silvina; Simon, Maria Rosa; Moreno, Maria Virginia; Santamarina Siurana, M. P.; Perello, Analia Edith; Endophytes from wheat as biocontrol agents against tan spot disease; Elsevier; Biological Control; 92; 9-2015; 17-231049-9644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1049964415300244info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2015.09.0021049-9644/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:52Zoai:ri.conicet.gov.ar:11336/27694instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:53.015CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endophytes from wheat as biocontrol agents against tan spot disease
title Endophytes from wheat as biocontrol agents against tan spot disease
spellingShingle Endophytes from wheat as biocontrol agents against tan spot disease
Larran, Silvina
Endophytes
Drechslera Tritici Repentis
Pyrenophora Tritici Repentis
Wheat
Endophytic Fungi
Tan Spot
Triticum Aestivum
title_short Endophytes from wheat as biocontrol agents against tan spot disease
title_full Endophytes from wheat as biocontrol agents against tan spot disease
title_fullStr Endophytes from wheat as biocontrol agents against tan spot disease
title_full_unstemmed Endophytes from wheat as biocontrol agents against tan spot disease
title_sort Endophytes from wheat as biocontrol agents against tan spot disease
dc.creator.none.fl_str_mv Larran, Silvina
Simon, Maria Rosa
Moreno, Maria Virginia
Santamarina Siurana, M. P.
Perello, Analia Edith
author Larran, Silvina
author_facet Larran, Silvina
Simon, Maria Rosa
Moreno, Maria Virginia
Santamarina Siurana, M. P.
Perello, Analia Edith
author_role author
author2 Simon, Maria Rosa
Moreno, Maria Virginia
Santamarina Siurana, M. P.
Perello, Analia Edith
author2_role author
author
author
author
dc.subject.none.fl_str_mv Endophytes
Drechslera Tritici Repentis
Pyrenophora Tritici Repentis
Wheat
Endophytic Fungi
Tan Spot
Triticum Aestivum
topic Endophytes
Drechslera Tritici Repentis
Pyrenophora Tritici Repentis
Wheat
Endophytic Fungi
Tan Spot
Triticum Aestivum
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against Pyrenophora tritici-repentis (Died.) Drechsler (anamorph Drechslera tritici-repentis) (Died.) Shoem (Dtr), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced Dtr growth compared to the control except Rhodotorula rubra from 11 to 15 days post inoculation. Trichoderma hamatum, Penicillium sp., Bacillus sp. and Paecilomyces lilacinus significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of D. tritici-repentis. In addition, two endophytes, Bacillus sp. and Fusarium sp., reduced significantly the percent spore germination of Dtr compared to the control by 82% and 52% respectively. In greenhouse experiment T. hamatum, Chaetomium globosum and Fusarium sp. significantly (P ⩽ 0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with T. hamatum as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise, Bacillus sp. was other highlighted microorganism that antagonized the pathogen in in vitro assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by D. tritici-repentis, particularly T. hamatum and Bacillus sp.
Fil: Larran, Silvina. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Simon, Maria Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Moreno, Maria Virginia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Santamarina Siurana, M. P.. Universidad Politécnica de Valencia; España
Fil: Perello, Analia Edith. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against Pyrenophora tritici-repentis (Died.) Drechsler (anamorph Drechslera tritici-repentis) (Died.) Shoem (Dtr), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced Dtr growth compared to the control except Rhodotorula rubra from 11 to 15 days post inoculation. Trichoderma hamatum, Penicillium sp., Bacillus sp. and Paecilomyces lilacinus significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of D. tritici-repentis. In addition, two endophytes, Bacillus sp. and Fusarium sp., reduced significantly the percent spore germination of Dtr compared to the control by 82% and 52% respectively. In greenhouse experiment T. hamatum, Chaetomium globosum and Fusarium sp. significantly (P ⩽ 0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with T. hamatum as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise, Bacillus sp. was other highlighted microorganism that antagonized the pathogen in in vitro assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by D. tritici-repentis, particularly T. hamatum and Bacillus sp.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27694
Larran, Silvina; Simon, Maria Rosa; Moreno, Maria Virginia; Santamarina Siurana, M. P.; Perello, Analia Edith; Endophytes from wheat as biocontrol agents against tan spot disease; Elsevier; Biological Control; 92; 9-2015; 17-23
1049-9644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27694
identifier_str_mv Larran, Silvina; Simon, Maria Rosa; Moreno, Maria Virginia; Santamarina Siurana, M. P.; Perello, Analia Edith; Endophytes from wheat as biocontrol agents against tan spot disease; Elsevier; Biological Control; 92; 9-2015; 17-23
1049-9644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1049964415300244
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2015.09.0021049-9644/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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