Endophytes from wheat as biocontrol agents against tan spot disease

Autores
Larran, S.; Simon, M.R.; Moreno, María Virginia; Santamarina Siurana, M.P.; Perelló, Analía
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents againstPyrenophora tritici-repentis(Died.) Drechsler (anamorphDrechslera tritici-repentis) (Died.) Shoem (Dtr), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reducedDtrgrowth compared to the control exceptRhodotorula rubrafrom 11 to 15days post inoculation.Trichoderma hamatum,Penicilliumsp.,Bacillussp. andPaecilomyces lilacinussignificantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia ofD. tritici-repentis. In addition, two endophytes,Bacillussp. andFusariumsp., reduced significantly the percent spore germination ofDtrcompared to the control by 82% and 52% respectively. In greenhouse experimentT. hamatum,Chaetomium globosumandFusariumsp. significantly (P⩽0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown withT. hamatumas it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise,Bacillussp. was other highlighted microorganism that antagonized the pathogen inin vitroassays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused byD. tritici-repentis, particularlyT. hamatumandBacillussp.
Materia
Agronomía, reproducción y protección de plantas
Endophytic fungi
Endophytes
Triticum aestivum
Pyrenophora tritici-repentis
Tan spot
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/6290

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/6290
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Endophytes from wheat as biocontrol agents against tan spot diseaseLarran, S.Simon, M.R.Moreno, María VirginiaSantamarina Siurana, M.P.Perelló, AnalíaAgronomía, reproducción y protección de plantasEndophytic fungiEndophytesTriticum aestivumPyrenophora tritici-repentisTan spotEndophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against<em>Pyrenophora tritici-repentis</em>(Died.) Drechsler (anamorph<em>Drechslera tritici-repentis</em>) (Died.) Shoem (<em>Dtr</em>), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced<em>Dtr</em>growth compared to the control except<em>Rhodotorula rubra</em>from 11 to 15days post inoculation.<em>Trichoderma hamatum</em>,<em>Penicillium</em>sp.,<em>Bacillus</em>sp. and<em>Paecilomyces lilacinus</em>significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of<em>D. tritici-repentis</em>. In addition, two endophytes,<em>Bacillus</em>sp. and<em>Fusarium</em>sp., reduced significantly the percent spore germination of<em>Dtr</em>compared to the control by 82% and 52% respectively. In greenhouse experiment<em>T. hamatum</em>,<em>Chaetomium globosum</em>and<em>Fusarium</em>sp. significantly (<em>P</em>⩽0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with<em>T. hamatum</em>as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise,<em>Bacillus</em>sp. was other highlighted microorganism that antagonized the pathogen in<em>in vitro</em>assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by<em>D. tritici-repentis</em>, particularly<em>T. hamatum</em>and<em>Bacillus</em>sp.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/6290enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2015.09.002info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:20Zoai:digital.cic.gba.gob.ar:11746/6290Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:20.677CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Endophytes from wheat as biocontrol agents against tan spot disease
title Endophytes from wheat as biocontrol agents against tan spot disease
spellingShingle Endophytes from wheat as biocontrol agents against tan spot disease
Larran, S.
Agronomía, reproducción y protección de plantas
Endophytic fungi
Endophytes
Triticum aestivum
Pyrenophora tritici-repentis
Tan spot
title_short Endophytes from wheat as biocontrol agents against tan spot disease
title_full Endophytes from wheat as biocontrol agents against tan spot disease
title_fullStr Endophytes from wheat as biocontrol agents against tan spot disease
title_full_unstemmed Endophytes from wheat as biocontrol agents against tan spot disease
title_sort Endophytes from wheat as biocontrol agents against tan spot disease
dc.creator.none.fl_str_mv Larran, S.
Simon, M.R.
Moreno, María Virginia
Santamarina Siurana, M.P.
Perelló, Analía
author Larran, S.
author_facet Larran, S.
Simon, M.R.
Moreno, María Virginia
Santamarina Siurana, M.P.
Perelló, Analía
author_role author
author2 Simon, M.R.
Moreno, María Virginia
Santamarina Siurana, M.P.
Perelló, Analía
author2_role author
author
author
author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
Endophytic fungi
Endophytes
Triticum aestivum
Pyrenophora tritici-repentis
Tan spot
topic Agronomía, reproducción y protección de plantas
Endophytic fungi
Endophytes
Triticum aestivum
Pyrenophora tritici-repentis
Tan spot
dc.description.none.fl_txt_mv Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against<em>Pyrenophora tritici-repentis</em>(Died.) Drechsler (anamorph<em>Drechslera tritici-repentis</em>) (Died.) Shoem (<em>Dtr</em>), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced<em>Dtr</em>growth compared to the control except<em>Rhodotorula rubra</em>from 11 to 15days post inoculation.<em>Trichoderma hamatum</em>,<em>Penicillium</em>sp.,<em>Bacillus</em>sp. and<em>Paecilomyces lilacinus</em>significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of<em>D. tritici-repentis</em>. In addition, two endophytes,<em>Bacillus</em>sp. and<em>Fusarium</em>sp., reduced significantly the percent spore germination of<em>Dtr</em>compared to the control by 82% and 52% respectively. In greenhouse experiment<em>T. hamatum</em>,<em>Chaetomium globosum</em>and<em>Fusarium</em>sp. significantly (<em>P</em>⩽0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with<em>T. hamatum</em>as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise,<em>Bacillus</em>sp. was other highlighted microorganism that antagonized the pathogen in<em>in vitro</em>assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by<em>D. tritici-repentis</em>, particularly<em>T. hamatum</em>and<em>Bacillus</em>sp.
description Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against<em>Pyrenophora tritici-repentis</em>(Died.) Drechsler (anamorph<em>Drechslera tritici-repentis</em>) (Died.) Shoem (<em>Dtr</em>), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced<em>Dtr</em>growth compared to the control except<em>Rhodotorula rubra</em>from 11 to 15days post inoculation.<em>Trichoderma hamatum</em>,<em>Penicillium</em>sp.,<em>Bacillus</em>sp. and<em>Paecilomyces lilacinus</em>significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of<em>D. tritici-repentis</em>. In addition, two endophytes,<em>Bacillus</em>sp. and<em>Fusarium</em>sp., reduced significantly the percent spore germination of<em>Dtr</em>compared to the control by 82% and 52% respectively. In greenhouse experiment<em>T. hamatum</em>,<em>Chaetomium globosum</em>and<em>Fusarium</em>sp. significantly (<em>P</em>⩽0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with<em>T. hamatum</em>as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise,<em>Bacillus</em>sp. was other highlighted microorganism that antagonized the pathogen in<em>in vitro</em>assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by<em>D. tritici-repentis</em>, particularly<em>T. hamatum</em>and<em>Bacillus</em>sp.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
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info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/6290
url https://digital.cic.gba.gob.ar/handle/11746/6290
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2015.09.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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