Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem

Autores
Huygens, D.; Diaz, Sandra Myrna; Urcelay, Roberto Carlos; Boeckx, P.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant uptake of dissolved organic nitrogen (DON) has been proposed to explain inconsistency in the ecosystem N balance of semi-arid systems. Nevertheless, direct evidence for an ecologically important role of DON in plant nutrition in these systems remains elusive under field conditions. Here, natural abundance 15N signatures of NO3−, NH4+, DON and whole plants from a semi-arid model forest were analyzed to provide robust estimates of plant N source partitioning and relative N cycling rates under in-situ conditions. Concurrently, architectural and symbiotic root traits were determined to assess their relationship to plant N acquisition strategies. Bayesian isotope mixing models indicated an insignificant contribution of DON to ecosystem plant N nutrition. Nitrate was the dominant plant N source in this ecosystem, while the contribution of NH4+ to plant nutrition varied between herbaceous (26%) and woody plants (8%). The low C:N ratio of the dissolved organic matter pool - ranging from 12.7 to 4.9 within the soil profile ? indicated microbial C-limitation in this ecosystem. Dissolved organic N was significantly enriched in 15N relative to NH4+ and NO3−, corroborating the importance of dissolved organic matter recycling as a cost-effective pathway that simultaneously supplies C and nutrients for microbial metabolism. Plants exclusively depend on inorganic N forms made available through microbial N mineralization and free-living atmospheric N2 fixation, followed by autotrophic nitrification.
Fil: Huygens, D.. Ghent University. Isotope Bioscience Laboratory; Países Bajos
Fil: Diaz, Sandra Myrna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Urcelay, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Boeckx, P.. Ghent University. Isotope Bioscience Laboratory; Países Bajos
Materia
Biological Nitrogen Fixation
Plant Microbial Competition
Chaco
Dissolved Organic Nitrogen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19267
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystemHuygens, D.Diaz, Sandra MyrnaUrcelay, Roberto CarlosBoeckx, P.Biological Nitrogen FixationPlant Microbial CompetitionChacoDissolved Organic Nitrogenhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant uptake of dissolved organic nitrogen (DON) has been proposed to explain inconsistency in the ecosystem N balance of semi-arid systems. Nevertheless, direct evidence for an ecologically important role of DON in plant nutrition in these systems remains elusive under field conditions. Here, natural abundance 15N signatures of NO3−, NH4+, DON and whole plants from a semi-arid model forest were analyzed to provide robust estimates of plant N source partitioning and relative N cycling rates under in-situ conditions. Concurrently, architectural and symbiotic root traits were determined to assess their relationship to plant N acquisition strategies. Bayesian isotope mixing models indicated an insignificant contribution of DON to ecosystem plant N nutrition. Nitrate was the dominant plant N source in this ecosystem, while the contribution of NH4+ to plant nutrition varied between herbaceous (26%) and woody plants (8%). The low C:N ratio of the dissolved organic matter pool - ranging from 12.7 to 4.9 within the soil profile ? indicated microbial C-limitation in this ecosystem. Dissolved organic N was significantly enriched in 15N relative to NH4+ and NO3−, corroborating the importance of dissolved organic matter recycling as a cost-effective pathway that simultaneously supplies C and nutrients for microbial metabolism. Plants exclusively depend on inorganic N forms made available through microbial N mineralization and free-living atmospheric N2 fixation, followed by autotrophic nitrification.Fil: Huygens, D.. Ghent University. Isotope Bioscience Laboratory; Países BajosFil: Diaz, Sandra Myrna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Urcelay, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Boeckx, P.. Ghent University. Isotope Bioscience Laboratory; Países BajosElsevier2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19267Huygens, D.; Diaz, Sandra Myrna; Urcelay, Roberto Carlos; Boeckx, P.; Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem; Elsevier; Soil Biology And Biochemistry; 101; 10-2016; 142-1510038-07171879-3428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0038071716301481info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2016.07.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:24Zoai:ri.conicet.gov.ar:11336/19267instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:24.924CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
title Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
spellingShingle Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
Huygens, D.
Biological Nitrogen Fixation
Plant Microbial Competition
Chaco
Dissolved Organic Nitrogen
title_short Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
title_full Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
title_fullStr Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
title_full_unstemmed Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
title_sort Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem
dc.creator.none.fl_str_mv Huygens, D.
Diaz, Sandra Myrna
Urcelay, Roberto Carlos
Boeckx, P.
author Huygens, D.
author_facet Huygens, D.
Diaz, Sandra Myrna
Urcelay, Roberto Carlos
Boeckx, P.
author_role author
author2 Diaz, Sandra Myrna
Urcelay, Roberto Carlos
Boeckx, P.
author2_role author
author
author
dc.subject.none.fl_str_mv Biological Nitrogen Fixation
Plant Microbial Competition
Chaco
Dissolved Organic Nitrogen
topic Biological Nitrogen Fixation
Plant Microbial Competition
Chaco
Dissolved Organic Nitrogen
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant uptake of dissolved organic nitrogen (DON) has been proposed to explain inconsistency in the ecosystem N balance of semi-arid systems. Nevertheless, direct evidence for an ecologically important role of DON in plant nutrition in these systems remains elusive under field conditions. Here, natural abundance 15N signatures of NO3−, NH4+, DON and whole plants from a semi-arid model forest were analyzed to provide robust estimates of plant N source partitioning and relative N cycling rates under in-situ conditions. Concurrently, architectural and symbiotic root traits were determined to assess their relationship to plant N acquisition strategies. Bayesian isotope mixing models indicated an insignificant contribution of DON to ecosystem plant N nutrition. Nitrate was the dominant plant N source in this ecosystem, while the contribution of NH4+ to plant nutrition varied between herbaceous (26%) and woody plants (8%). The low C:N ratio of the dissolved organic matter pool - ranging from 12.7 to 4.9 within the soil profile ? indicated microbial C-limitation in this ecosystem. Dissolved organic N was significantly enriched in 15N relative to NH4+ and NO3−, corroborating the importance of dissolved organic matter recycling as a cost-effective pathway that simultaneously supplies C and nutrients for microbial metabolism. Plants exclusively depend on inorganic N forms made available through microbial N mineralization and free-living atmospheric N2 fixation, followed by autotrophic nitrification.
Fil: Huygens, D.. Ghent University. Isotope Bioscience Laboratory; Países Bajos
Fil: Diaz, Sandra Myrna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Urcelay, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Boeckx, P.. Ghent University. Isotope Bioscience Laboratory; Países Bajos
description Plant uptake of dissolved organic nitrogen (DON) has been proposed to explain inconsistency in the ecosystem N balance of semi-arid systems. Nevertheless, direct evidence for an ecologically important role of DON in plant nutrition in these systems remains elusive under field conditions. Here, natural abundance 15N signatures of NO3−, NH4+, DON and whole plants from a semi-arid model forest were analyzed to provide robust estimates of plant N source partitioning and relative N cycling rates under in-situ conditions. Concurrently, architectural and symbiotic root traits were determined to assess their relationship to plant N acquisition strategies. Bayesian isotope mixing models indicated an insignificant contribution of DON to ecosystem plant N nutrition. Nitrate was the dominant plant N source in this ecosystem, while the contribution of NH4+ to plant nutrition varied between herbaceous (26%) and woody plants (8%). The low C:N ratio of the dissolved organic matter pool - ranging from 12.7 to 4.9 within the soil profile ? indicated microbial C-limitation in this ecosystem. Dissolved organic N was significantly enriched in 15N relative to NH4+ and NO3−, corroborating the importance of dissolved organic matter recycling as a cost-effective pathway that simultaneously supplies C and nutrients for microbial metabolism. Plants exclusively depend on inorganic N forms made available through microbial N mineralization and free-living atmospheric N2 fixation, followed by autotrophic nitrification.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19267
Huygens, D.; Diaz, Sandra Myrna; Urcelay, Roberto Carlos; Boeckx, P.; Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem; Elsevier; Soil Biology And Biochemistry; 101; 10-2016; 142-151
0038-0717
1879-3428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19267
identifier_str_mv Huygens, D.; Diaz, Sandra Myrna; Urcelay, Roberto Carlos; Boeckx, P.; Microbial recycling of dissolved organic matter confines plant nitrogen uptake to inorganic forms in a semi-arid ecosystem; Elsevier; Soil Biology And Biochemistry; 101; 10-2016; 142-151
0038-0717
1879-3428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0038071716301481
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2016.07.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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