A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload

Autores
Piloni, Natacha Estefanía; Puntarulo, Susana Ángela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
All biological systems, contain many antioxidants; including water-soluble compounds, such as ascorbic acid. The one-electron oxidation of ascorbate (AH¯produces the ascorbyl radical (A•), that has a relatively long lifetime compared to other species, such as hydroxyl radical, peroxyl, alkoxyl, and carbon-centered lipid free radicals. A• has been proposed as a marker of oxidative stress either in vitro, or in vivo in numerous systems. The application of a simple kinetic analysis allowed us to estimate the steady state concentration of A•. These data were successfully compared to experimental values obtained using Electron Paramagnetic Resonance (EPR) in rat brain under physiological conditions. The model was also applied to estimate the response to stress due to subchronic Fe overload.
Fil: Piloni, Natacha Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
Ascorbyl radical
Brain
Kinetic model
Iron
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47511
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe OverloadPiloni, Natacha EstefaníaPuntarulo, Susana ÁngelaAscorbyl radicalBrainKinetic modelIronhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1All biological systems, contain many antioxidants; including water-soluble compounds, such as ascorbic acid. The one-electron oxidation of ascorbate (AH¯produces the ascorbyl radical (A•), that has a relatively long lifetime compared to other species, such as hydroxyl radical, peroxyl, alkoxyl, and carbon-centered lipid free radicals. A• has been proposed as a marker of oxidative stress either in vitro, or in vivo in numerous systems. The application of a simple kinetic analysis allowed us to estimate the steady state concentration of A•. These data were successfully compared to experimental values obtained using Electron Paramagnetic Resonance (EPR) in rat brain under physiological conditions. The model was also applied to estimate the response to stress due to subchronic Fe overload.Fil: Piloni, Natacha Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaOMICS International2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47511Piloni, Natacha Estefanía; Puntarulo, Susana Ángela; A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload; OMICS International; Bioenergetics: Open Access; 5; 2; 11-2016; 1-3; 1252167-7662CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2167-7662.1000e125info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/a-simple-kinetic-model-to-estimate-ascorbyl-radical-steady-state-concentration-in-rat-central-nervous-system-effect-of-subchronic-2167-7662-1000e125.php?aid=83544info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:19:26Zoai:ri.conicet.gov.ar:11336/47511instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:19:27.284CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
title A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
spellingShingle A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
Piloni, Natacha Estefanía
Ascorbyl radical
Brain
Kinetic model
Iron
title_short A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
title_full A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
title_fullStr A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
title_full_unstemmed A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
title_sort A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload
dc.creator.none.fl_str_mv Piloni, Natacha Estefanía
Puntarulo, Susana Ángela
author Piloni, Natacha Estefanía
author_facet Piloni, Natacha Estefanía
Puntarulo, Susana Ángela
author_role author
author2 Puntarulo, Susana Ángela
author2_role author
dc.subject.none.fl_str_mv Ascorbyl radical
Brain
Kinetic model
Iron
topic Ascorbyl radical
Brain
Kinetic model
Iron
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv All biological systems, contain many antioxidants; including water-soluble compounds, such as ascorbic acid. The one-electron oxidation of ascorbate (AH¯produces the ascorbyl radical (A•), that has a relatively long lifetime compared to other species, such as hydroxyl radical, peroxyl, alkoxyl, and carbon-centered lipid free radicals. A• has been proposed as a marker of oxidative stress either in vitro, or in vivo in numerous systems. The application of a simple kinetic analysis allowed us to estimate the steady state concentration of A•. These data were successfully compared to experimental values obtained using Electron Paramagnetic Resonance (EPR) in rat brain under physiological conditions. The model was also applied to estimate the response to stress due to subchronic Fe overload.
Fil: Piloni, Natacha Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description All biological systems, contain many antioxidants; including water-soluble compounds, such as ascorbic acid. The one-electron oxidation of ascorbate (AH¯produces the ascorbyl radical (A•), that has a relatively long lifetime compared to other species, such as hydroxyl radical, peroxyl, alkoxyl, and carbon-centered lipid free radicals. A• has been proposed as a marker of oxidative stress either in vitro, or in vivo in numerous systems. The application of a simple kinetic analysis allowed us to estimate the steady state concentration of A•. These data were successfully compared to experimental values obtained using Electron Paramagnetic Resonance (EPR) in rat brain under physiological conditions. The model was also applied to estimate the response to stress due to subchronic Fe overload.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47511
Piloni, Natacha Estefanía; Puntarulo, Susana Ángela; A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload; OMICS International; Bioenergetics: Open Access; 5; 2; 11-2016; 1-3; 125
2167-7662
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47511
identifier_str_mv Piloni, Natacha Estefanía; Puntarulo, Susana Ángela; A Simple Kinetic Model to Estimate Ascorbyl Radical Steady State Concentration in Rat Central Nervous System. Effect of Subchronic Fe Overload; OMICS International; Bioenergetics: Open Access; 5; 2; 11-2016; 1-3; 125
2167-7662
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2167-7662.1000e125
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/a-simple-kinetic-model-to-estimate-ascorbyl-radical-steady-state-concentration-in-rat-central-nervous-system-effect-of-subchronic-2167-7662-1000e125.php?aid=83544
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS International
publisher.none.fl_str_mv OMICS International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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