Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes

Autores
Grasso, Daniel Hector; Sacchetti, Maria L.; Bruno, Lidia; Lo Ré, Andrea Emilia; lovanna, Juan L.; González, Claudio Daniel; Vaccaro, Maria Ines
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Aims: We have described VMP1 as a new protein which expression triggers autophagy in mammalian cells. Here we show that experimental diabetes activates VMP1 expression and autophagy in pancreas beta cells as a direct response to streptozotocin (STZ). Methods: Male Wistar rats were treated with 65 mg/kg STZ and pancreas islets from untreated rats were incubated with 1 mM STZ. Results: RT-PCR analysis shows early VMP1 induction after STZ treatment. In situ hybridization reveals VMP1 mRNA in islet beta cells. Electron microscopy shows chromatin aggregation and autophagy morphology that was confirmed by LC3 expression and LC3-VMP1 co-localization. Apoptotic cell death and the reduction of beta cell pool are evident after 24 h treatment, while VMP1 is still expressed in the remaining cells.VMPI-Beclini colocalization in pancreastissuefrom STZ-treated rats suggests that VMPI-Beclini interaction is involved in the autophagic process activation during experimental diabetes. Results were confirmed using pancreas islets, showing VMP1 expression and autophagy in beta cells as a direct effect of STZ treatment. Conclusion: Pancreas beta cells trigger VMP1 expression and autophagy during the early cellular events in response to experimental diabetes.
Fil: Grasso, Daniel Hector. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sacchetti, Maria L.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Bruno, Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Lo Ré, Andrea Emilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: lovanna, Juan L.. Inserm; Francia
Fil: González, Claudio Daniel. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: Vaccaro, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Materia
PANCREAS
DIABETES
AUTOPHAGY
VMP1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242091
Acceder
id CONICETDig_2b6383b1cff64a3cf90a472cc2ddf663
oai_identifier_str oai:ri.conicet.gov.ar:11336/242091
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Autophagy and VMP1 Expression Are Early Cellular Events in Experimental DiabetesGrasso, Daniel HectorSacchetti, Maria L.Bruno, LidiaLo Ré, Andrea Emilialovanna, Juan L.González, Claudio DanielVaccaro, Maria InesPANCREASDIABETESAUTOPHAGYVMP1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background/Aims: We have described VMP1 as a new protein which expression triggers autophagy in mammalian cells. Here we show that experimental diabetes activates VMP1 expression and autophagy in pancreas beta cells as a direct response to streptozotocin (STZ). Methods: Male Wistar rats were treated with 65 mg/kg STZ and pancreas islets from untreated rats were incubated with 1 mM STZ. Results: RT-PCR analysis shows early VMP1 induction after STZ treatment. In situ hybridization reveals VMP1 mRNA in islet beta cells. Electron microscopy shows chromatin aggregation and autophagy morphology that was confirmed by LC3 expression and LC3-VMP1 co-localization. Apoptotic cell death and the reduction of beta cell pool are evident after 24 h treatment, while VMP1 is still expressed in the remaining cells.VMPI-Beclini colocalization in pancreastissuefrom STZ-treated rats suggests that VMPI-Beclini interaction is involved in the autophagic process activation during experimental diabetes. Results were confirmed using pancreas islets, showing VMP1 expression and autophagy in beta cells as a direct effect of STZ treatment. Conclusion: Pancreas beta cells trigger VMP1 expression and autophagy during the early cellular events in response to experimental diabetes.Fil: Grasso, Daniel Hector. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sacchetti, Maria L.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; ArgentinaFil: Bruno, Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; ArgentinaFil: Lo Ré, Andrea Emilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: lovanna, Juan L.. Inserm; FranciaFil: González, Claudio Daniel. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; ArgentinaFil: Vaccaro, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; ArgentinaKarger2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242091Grasso, Daniel Hector; Sacchetti, Maria L.; Bruno, Lidia; Lo Ré, Andrea Emilia; lovanna, Juan L.; et al.; Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes; Karger; Pancreatology; 9; 1-2; 12-2009; 81-881424-3903CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000178878info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1424390309800732info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:42Zoai:ri.conicet.gov.ar:11336/242091instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:43.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
title Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
spellingShingle Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
Grasso, Daniel Hector
PANCREAS
DIABETES
AUTOPHAGY
VMP1
title_short Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
title_full Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
title_fullStr Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
title_full_unstemmed Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
title_sort Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes
dc.creator.none.fl_str_mv Grasso, Daniel Hector
Sacchetti, Maria L.
Bruno, Lidia
Lo Ré, Andrea Emilia
lovanna, Juan L.
González, Claudio Daniel
Vaccaro, Maria Ines
author Grasso, Daniel Hector
author_facet Grasso, Daniel Hector
Sacchetti, Maria L.
Bruno, Lidia
Lo Ré, Andrea Emilia
lovanna, Juan L.
González, Claudio Daniel
Vaccaro, Maria Ines
author_role author
author2 Sacchetti, Maria L.
Bruno, Lidia
Lo Ré, Andrea Emilia
lovanna, Juan L.
González, Claudio Daniel
Vaccaro, Maria Ines
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PANCREAS
DIABETES
AUTOPHAGY
VMP1
topic PANCREAS
DIABETES
AUTOPHAGY
VMP1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background/Aims: We have described VMP1 as a new protein which expression triggers autophagy in mammalian cells. Here we show that experimental diabetes activates VMP1 expression and autophagy in pancreas beta cells as a direct response to streptozotocin (STZ). Methods: Male Wistar rats were treated with 65 mg/kg STZ and pancreas islets from untreated rats were incubated with 1 mM STZ. Results: RT-PCR analysis shows early VMP1 induction after STZ treatment. In situ hybridization reveals VMP1 mRNA in islet beta cells. Electron microscopy shows chromatin aggregation and autophagy morphology that was confirmed by LC3 expression and LC3-VMP1 co-localization. Apoptotic cell death and the reduction of beta cell pool are evident after 24 h treatment, while VMP1 is still expressed in the remaining cells.VMPI-Beclini colocalization in pancreastissuefrom STZ-treated rats suggests that VMPI-Beclini interaction is involved in the autophagic process activation during experimental diabetes. Results were confirmed using pancreas islets, showing VMP1 expression and autophagy in beta cells as a direct effect of STZ treatment. Conclusion: Pancreas beta cells trigger VMP1 expression and autophagy during the early cellular events in response to experimental diabetes.
Fil: Grasso, Daniel Hector. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sacchetti, Maria L.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Bruno, Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Lo Ré, Andrea Emilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: lovanna, Juan L.. Inserm; Francia
Fil: González, Claudio Daniel. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: Vaccaro, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
description Background/Aims: We have described VMP1 as a new protein which expression triggers autophagy in mammalian cells. Here we show that experimental diabetes activates VMP1 expression and autophagy in pancreas beta cells as a direct response to streptozotocin (STZ). Methods: Male Wistar rats were treated with 65 mg/kg STZ and pancreas islets from untreated rats were incubated with 1 mM STZ. Results: RT-PCR analysis shows early VMP1 induction after STZ treatment. In situ hybridization reveals VMP1 mRNA in islet beta cells. Electron microscopy shows chromatin aggregation and autophagy morphology that was confirmed by LC3 expression and LC3-VMP1 co-localization. Apoptotic cell death and the reduction of beta cell pool are evident after 24 h treatment, while VMP1 is still expressed in the remaining cells.VMPI-Beclini colocalization in pancreastissuefrom STZ-treated rats suggests that VMPI-Beclini interaction is involved in the autophagic process activation during experimental diabetes. Results were confirmed using pancreas islets, showing VMP1 expression and autophagy in beta cells as a direct effect of STZ treatment. Conclusion: Pancreas beta cells trigger VMP1 expression and autophagy during the early cellular events in response to experimental diabetes.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242091
Grasso, Daniel Hector; Sacchetti, Maria L.; Bruno, Lidia; Lo Ré, Andrea Emilia; lovanna, Juan L.; et al.; Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes; Karger; Pancreatology; 9; 1-2; 12-2009; 81-88
1424-3903
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242091
identifier_str_mv Grasso, Daniel Hector; Sacchetti, Maria L.; Bruno, Lidia; Lo Ré, Andrea Emilia; lovanna, Juan L.; et al.; Autophagy and VMP1 Expression Are Early Cellular Events in Experimental Diabetes; Karger; Pancreatology; 9; 1-2; 12-2009; 81-88
1424-3903
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000178878
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1424390309800732
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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