Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina

Autores
Casero, Rodolfo Daniel; Mongi, Florencia; Laconte, Laura; Rivero, Fernando David; Sastre, Dario; Teherán, Aníbal; Herrera, Giovanny; Ramírez, Juan David
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection.
Fil: Casero, Rodolfo Daniel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Mongi, Florencia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Laconte, Laura. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Rivero, Fernando David. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sastre, Dario. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Teherán, Aníbal. Universidad Colegio Mayor de Nuestra Señora del Rosario; Colombia
Fil: Herrera, Giovanny. Universidad Colegio Mayor de Nuestra Señora del Rosario; Colombia
Fil: Ramírez, Juan David. Universidad Colegio Mayor de Nuestra Señora del Rosario; Colombia
Materia
Acanthamoeba
Argentina
Contact Lens
Genotype
Keratitis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41217
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41217
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in ArgentinaCasero, Rodolfo DanielMongi, FlorenciaLaconte, LauraRivero, Fernando DavidSastre, DarioTeherán, AníbalHerrera, GiovannyRamírez, Juan DavidAcanthamoebaArgentinaContact LensGenotypeKeratitishttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection.Fil: Casero, Rodolfo Daniel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Mongi, Florencia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Laconte, Laura. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Rivero, Fernando David. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sastre, Dario. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Teherán, Aníbal. Universidad Colegio Mayor de Nuestra Señora del Rosario; ColombiaFil: Herrera, Giovanny. Universidad Colegio Mayor de Nuestra Señora del Rosario; ColombiaFil: Ramírez, Juan David. Universidad Colegio Mayor de Nuestra Señora del Rosario; ColombiaElsevier Science2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41217Casero, Rodolfo Daniel; Mongi, Florencia; Laconte, Laura; Rivero, Fernando David; Sastre, Dario; et al.; Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina; Elsevier Science; Infection, Genetics and Evolution; 54; 10-2017; 170-1751567-1348CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2017.06.031info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134817302290info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:52Zoai:ri.conicet.gov.ar:11336/41217instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:52.904CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
spellingShingle Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
Casero, Rodolfo Daniel
Acanthamoeba
Argentina
Contact Lens
Genotype
Keratitis
title_short Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_full Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_fullStr Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_full_unstemmed Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
title_sort Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina
dc.creator.none.fl_str_mv Casero, Rodolfo Daniel
Mongi, Florencia
Laconte, Laura
Rivero, Fernando David
Sastre, Dario
Teherán, Aníbal
Herrera, Giovanny
Ramírez, Juan David
author Casero, Rodolfo Daniel
author_facet Casero, Rodolfo Daniel
Mongi, Florencia
Laconte, Laura
Rivero, Fernando David
Sastre, Dario
Teherán, Aníbal
Herrera, Giovanny
Ramírez, Juan David
author_role author
author2 Mongi, Florencia
Laconte, Laura
Rivero, Fernando David
Sastre, Dario
Teherán, Aníbal
Herrera, Giovanny
Ramírez, Juan David
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Acanthamoeba
Argentina
Contact Lens
Genotype
Keratitis
topic Acanthamoeba
Argentina
Contact Lens
Genotype
Keratitis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection.
Fil: Casero, Rodolfo Daniel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Mongi, Florencia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Laconte, Laura. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Rivero, Fernando David. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sastre, Dario. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Teherán, Aníbal. Universidad Colegio Mayor de Nuestra Señora del Rosario; Colombia
Fil: Herrera, Giovanny. Universidad Colegio Mayor de Nuestra Señora del Rosario; Colombia
Fil: Ramírez, Juan David. Universidad Colegio Mayor de Nuestra Señora del Rosario; Colombia
description In this study, we describe the frequency of Acanthamoeba keratitis (AK) in patients that assisted in the Ophthalmology Department and determine the species/genotypes of free living amoebas (FLA) isolates. FLA from Corneal scrapes (CS) and contact lens (CL) wearers were studied by morphological and molecular characterization. A database was constructed with sociodemographic, clinical findings and history of use of CL variables. During January 2000 and September 2016 patients with corneal pathology admitted to the Ophthalmology Service of the University Hospital in Córdoba city, Argentina were included in the study. FLA were detected in 1.5% (11/739) and in 17% (11/65) of CS and CL analyzed respectively. FLA isolates from CL users evidenced an 80.9% of inappropriate lens maintenance, 4.8% (1/21) were not CL users that have been in contact with waters in outdoor environment and 14,3% (3/21) with no data about CL users. Acanthamoeba was confirmed in 100% and 82% of CS and LC respectively. The most frequent symptom associated with AK was red eye and photophobia. FLA from CS belonged to group II but 82% (9/11) and 18% (2/11) from CL belonged to group II and III respectively. T4 genotype and A. polyphaga species were detected in 100% of Acanthamoeba isolates. Poor CL hygiene practices, highlights the need for improved education about the severity of AK and consequences of improper CL hygiene. Genotype T4 detected in 100% of both CS and CL samples, consistently with previous findings indicating that this genotype is by far the most prevalent isolated from ocular infection.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41217
Casero, Rodolfo Daniel; Mongi, Florencia; Laconte, Laura; Rivero, Fernando David; Sastre, Dario; et al.; Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina; Elsevier Science; Infection, Genetics and Evolution; 54; 10-2017; 170-175
1567-1348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41217
identifier_str_mv Casero, Rodolfo Daniel; Mongi, Florencia; Laconte, Laura; Rivero, Fernando David; Sastre, Dario; et al.; Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina; Elsevier Science; Infection, Genetics and Evolution; 54; 10-2017; 170-175
1567-1348
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2017.06.031
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134817302290
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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