Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?

Autores
Padula, Gisel; Ponzinibbio, Maria Virginia; Gambaro, Rocío Celeste; Seoane, Analia Isabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Micronutrients are important for the prevention of degenerative diseases due to their role in maintaining genomic stability. Therefore, there is international concern about the need to redefine the optimal mineral and vitamin requirements to prevent DNA damage. We analyzed the cytostatic, cytotoxic, and genotoxic effect of in vitro zinc supplementation to determine the effects of zinc deficiency and excess and whether the upper estimate of the physiological requirement recommended for children is safe. To achieve zinc deficiency, DMEM/Ham’s F12 medium (HF12) was chelated (HF12Q). Lymphocytes were isolated from healthy female donors (age range, 5–10 yr) and cultured for 7 d as follows: negative control (HF12, 60 μg/dl ZnSO4); deficient (HF12Q, 12 μg/dl ZnSO4); lower level (HF12Q + 80 μg/dl ZnSO4); average level (HF12Q + 180 μg/dl ZnSO4); upper limit (HF12Q + 280 μg/dl ZnSO4); and excess (HF12Q + 380 μg/dl ZnSO4). The comet (quantitative analysis) and cytokinesis-block micronucleus cytome assays were used. Differences were evaluated with Kruskal-Wallis and ANOVA (p < 0.05). Olive tail moment, tail length, micronuclei frequency, and apoptotic and necrotic percentages were significantly higher in the deficient, upper limit, and excess cultures compared with the negative control, lower, and average limit ones. In vitro zinc supplementation at the lower and average limit (80 and 180 μg/dl ZnSO4) of the physiological requirement recommended for children proved to be the most beneficial in avoiding genomic instability, whereas the deficient, upper limit, and excess (12, 280, and 380 μg/dl) cultures increased DNA and chromosomal damage and apoptotic and necrotic frequencies.
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
Fil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
Materia
CHILDREN
GENOMIC STABILITY
HEALTH
RECOMMENDED DIETARY ALLOWANCES
ZINC SUPPLEMENTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62619
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62619
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?Padula, GiselPonzinibbio, Maria VirginiaGambaro, Rocío CelesteSeoane, Analia IsabelCHILDRENGENOMIC STABILITYHEALTHRECOMMENDED DIETARY ALLOWANCESZINC SUPPLEMENTATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Micronutrients are important for the prevention of degenerative diseases due to their role in maintaining genomic stability. Therefore, there is international concern about the need to redefine the optimal mineral and vitamin requirements to prevent DNA damage. We analyzed the cytostatic, cytotoxic, and genotoxic effect of in vitro zinc supplementation to determine the effects of zinc deficiency and excess and whether the upper estimate of the physiological requirement recommended for children is safe. To achieve zinc deficiency, DMEM/Ham’s F12 medium (HF12) was chelated (HF12Q). Lymphocytes were isolated from healthy female donors (age range, 5–10 yr) and cultured for 7 d as follows: negative control (HF12, 60 μg/dl ZnSO4); deficient (HF12Q, 12 μg/dl ZnSO4); lower level (HF12Q + 80 μg/dl ZnSO4); average level (HF12Q + 180 μg/dl ZnSO4); upper limit (HF12Q + 280 μg/dl ZnSO4); and excess (HF12Q + 380 μg/dl ZnSO4). The comet (quantitative analysis) and cytokinesis-block micronucleus cytome assays were used. Differences were evaluated with Kruskal-Wallis and ANOVA (p < 0.05). Olive tail moment, tail length, micronuclei frequency, and apoptotic and necrotic percentages were significantly higher in the deficient, upper limit, and excess cultures compared with the negative control, lower, and average limit ones. In vitro zinc supplementation at the lower and average limit (80 and 180 μg/dl ZnSO4) of the physiological requirement recommended for children proved to be the most beneficial in avoiding genomic instability, whereas the deficient, upper limit, and excess (12, 280, and 380 μg/dl) cultures increased DNA and chromosomal damage and apoptotic and necrotic frequencies.Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; ArgentinaFil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; ArgentinaFil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; ArgentinaSpringer2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62619Padula, Gisel; Ponzinibbio, Maria Virginia; Gambaro, Rocío Celeste; Seoane, Analia Isabel; Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?; Springer; In Vitro Cellular & Developmental Biology. Animal.; 53; 7; 8-2017; 586-5921071-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11626-017-0146-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11626-017-0146-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:57Zoai:ri.conicet.gov.ar:11336/62619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:58.347CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
title Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
spellingShingle Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
Padula, Gisel
CHILDREN
GENOMIC STABILITY
HEALTH
RECOMMENDED DIETARY ALLOWANCES
ZINC SUPPLEMENTATION
title_short Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
title_full Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
title_fullStr Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
title_full_unstemmed Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
title_sort Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?
dc.creator.none.fl_str_mv Padula, Gisel
Ponzinibbio, Maria Virginia
Gambaro, Rocío Celeste
Seoane, Analia Isabel
author Padula, Gisel
author_facet Padula, Gisel
Ponzinibbio, Maria Virginia
Gambaro, Rocío Celeste
Seoane, Analia Isabel
author_role author
author2 Ponzinibbio, Maria Virginia
Gambaro, Rocío Celeste
Seoane, Analia Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv CHILDREN
GENOMIC STABILITY
HEALTH
RECOMMENDED DIETARY ALLOWANCES
ZINC SUPPLEMENTATION
topic CHILDREN
GENOMIC STABILITY
HEALTH
RECOMMENDED DIETARY ALLOWANCES
ZINC SUPPLEMENTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Micronutrients are important for the prevention of degenerative diseases due to their role in maintaining genomic stability. Therefore, there is international concern about the need to redefine the optimal mineral and vitamin requirements to prevent DNA damage. We analyzed the cytostatic, cytotoxic, and genotoxic effect of in vitro zinc supplementation to determine the effects of zinc deficiency and excess and whether the upper estimate of the physiological requirement recommended for children is safe. To achieve zinc deficiency, DMEM/Ham’s F12 medium (HF12) was chelated (HF12Q). Lymphocytes were isolated from healthy female donors (age range, 5–10 yr) and cultured for 7 d as follows: negative control (HF12, 60 μg/dl ZnSO4); deficient (HF12Q, 12 μg/dl ZnSO4); lower level (HF12Q + 80 μg/dl ZnSO4); average level (HF12Q + 180 μg/dl ZnSO4); upper limit (HF12Q + 280 μg/dl ZnSO4); and excess (HF12Q + 380 μg/dl ZnSO4). The comet (quantitative analysis) and cytokinesis-block micronucleus cytome assays were used. Differences were evaluated with Kruskal-Wallis and ANOVA (p < 0.05). Olive tail moment, tail length, micronuclei frequency, and apoptotic and necrotic percentages were significantly higher in the deficient, upper limit, and excess cultures compared with the negative control, lower, and average limit ones. In vitro zinc supplementation at the lower and average limit (80 and 180 μg/dl ZnSO4) of the physiological requirement recommended for children proved to be the most beneficial in avoiding genomic instability, whereas the deficient, upper limit, and excess (12, 280, and 380 μg/dl) cultures increased DNA and chromosomal damage and apoptotic and necrotic frequencies.
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
Fil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
description Micronutrients are important for the prevention of degenerative diseases due to their role in maintaining genomic stability. Therefore, there is international concern about the need to redefine the optimal mineral and vitamin requirements to prevent DNA damage. We analyzed the cytostatic, cytotoxic, and genotoxic effect of in vitro zinc supplementation to determine the effects of zinc deficiency and excess and whether the upper estimate of the physiological requirement recommended for children is safe. To achieve zinc deficiency, DMEM/Ham’s F12 medium (HF12) was chelated (HF12Q). Lymphocytes were isolated from healthy female donors (age range, 5–10 yr) and cultured for 7 d as follows: negative control (HF12, 60 μg/dl ZnSO4); deficient (HF12Q, 12 μg/dl ZnSO4); lower level (HF12Q + 80 μg/dl ZnSO4); average level (HF12Q + 180 μg/dl ZnSO4); upper limit (HF12Q + 280 μg/dl ZnSO4); and excess (HF12Q + 380 μg/dl ZnSO4). The comet (quantitative analysis) and cytokinesis-block micronucleus cytome assays were used. Differences were evaluated with Kruskal-Wallis and ANOVA (p < 0.05). Olive tail moment, tail length, micronuclei frequency, and apoptotic and necrotic percentages were significantly higher in the deficient, upper limit, and excess cultures compared with the negative control, lower, and average limit ones. In vitro zinc supplementation at the lower and average limit (80 and 180 μg/dl ZnSO4) of the physiological requirement recommended for children proved to be the most beneficial in avoiding genomic instability, whereas the deficient, upper limit, and excess (12, 280, and 380 μg/dl) cultures increased DNA and chromosomal damage and apoptotic and necrotic frequencies.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62619
Padula, Gisel; Ponzinibbio, Maria Virginia; Gambaro, Rocío Celeste; Seoane, Analia Isabel; Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?; Springer; In Vitro Cellular & Developmental Biology. Animal.; 53; 7; 8-2017; 586-592
1071-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62619
identifier_str_mv Padula, Gisel; Ponzinibbio, Maria Virginia; Gambaro, Rocío Celeste; Seoane, Analia Isabel; Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?; Springer; In Vitro Cellular & Developmental Biology. Animal.; 53; 7; 8-2017; 586-592
1071-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11626-017-0146-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11626-017-0146-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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