Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System

Autores
Mazzeo, Marcelo Adrián; Linares, Jorge Alberto; López, María Marcela; Bachmeier, Evelin; Wietz, Fernando Martín; Galván Josa, Víctor Martín; Valentinuzzi, Maria Cecilia; Riveros de la Vega, Jose Alberto; Finkelberg, Ana Beatriz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work presents a chemical and morphological analysis of samples of saliva taken from patients who were under treatment with intravenous chemotherapy with 5-fluorouracil and leucovorin calcium. Samples of saliva were extracted from fifteen patients during the three stages of the treatment: The initial stage (previous to the chemotherapy), the intermediate stage (during the chemotherapy), and the final stage (twenty-one days after finishing the treatment). An amount of 50 ll was collected in each visit. Chemical contrast images were taken by means of scanning electron microscopy, and X-ray characteristic spectra were obtained from all the studied samples by using an energy dispersive system from all the studied samples. Images that correspond to the intermediate stage showed important differences with respect to the initial and final stages. In addition, X-ray spectra provided information about the present elements in saliva and their relative abundance allowed us to determine variations in the chemical composition. The backscattered electron images and X-ray spectra from the intermediate stage showed clusters of crystals with fluorine content higher than those obtained in initial and final stages. This fact probably indicates the passage of metabolites of 5-fluorouracil and leucovorin calcium from the plasma to the oral cavity. This finding enhances the hypothesis proposed by other authors about the secondary effects of the drugs on the stomatognathic system such as oral mucositis, dysgeusia, and xerostomia with or without hyposalivation.
Fil: Mazzeo, Marcelo Adrián. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Linares, Jorge Alberto. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: López, María Marcela. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Bachmeier, Evelin. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Wietz, Fernando Martín. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Galván Josa, Víctor Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Valentinuzzi, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Riveros de la Vega, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Finkelberg, Ana Beatriz. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Materia
5 Fluorouracil
Saliva
Sem-Eds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11041
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11041
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive SystemMazzeo, Marcelo AdriánLinares, Jorge AlbertoLópez, María MarcelaBachmeier, EvelinWietz, Fernando MartínGalván Josa, Víctor MartínValentinuzzi, Maria CeciliaRiveros de la Vega, Jose AlbertoFinkelberg, Ana Beatriz5 FluorouracilSalivaSem-Edshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3This work presents a chemical and morphological analysis of samples of saliva taken from patients who were under treatment with intravenous chemotherapy with 5-fluorouracil and leucovorin calcium. Samples of saliva were extracted from fifteen patients during the three stages of the treatment: The initial stage (previous to the chemotherapy), the intermediate stage (during the chemotherapy), and the final stage (twenty-one days after finishing the treatment). An amount of 50 ll was collected in each visit. Chemical contrast images were taken by means of scanning electron microscopy, and X-ray characteristic spectra were obtained from all the studied samples by using an energy dispersive system from all the studied samples. Images that correspond to the intermediate stage showed important differences with respect to the initial and final stages. In addition, X-ray spectra provided information about the present elements in saliva and their relative abundance allowed us to determine variations in the chemical composition. The backscattered electron images and X-ray spectra from the intermediate stage showed clusters of crystals with fluorine content higher than those obtained in initial and final stages. This fact probably indicates the passage of metabolites of 5-fluorouracil and leucovorin calcium from the plasma to the oral cavity. This finding enhances the hypothesis proposed by other authors about the secondary effects of the drugs on the stomatognathic system such as oral mucositis, dysgeusia, and xerostomia with or without hyposalivation.Fil: Mazzeo, Marcelo Adrián. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; ArgentinaFil: Linares, Jorge Alberto. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; ArgentinaFil: López, María Marcela. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; ArgentinaFil: Bachmeier, Evelin. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; ArgentinaFil: Wietz, Fernando Martín. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; ArgentinaFil: Galván Josa, Víctor Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Valentinuzzi, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Riveros de la Vega, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Finkelberg, Ana Beatriz. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; ArgentinaWiley2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11041Mazzeo, Marcelo Adrián; Linares, Jorge Alberto; López, María Marcela; Bachmeier, Evelin; Wietz, Fernando Martín; et al.; Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System; Wiley; Journal Of Oral Pathology And Medicine; 42; 10; 11-2013; 788-7920904-25121600-0714enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jop.12078/fullinfo:eu-repo/semantics/altIdentifier/doi/10.1111/jop.12078info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:13:23Zoai:ri.conicet.gov.ar:11336/11041instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:13:23.284CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
title Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
spellingShingle Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
Mazzeo, Marcelo Adrián
5 Fluorouracil
Saliva
Sem-Eds
title_short Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
title_full Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
title_fullStr Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
title_full_unstemmed Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
title_sort Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System
dc.creator.none.fl_str_mv Mazzeo, Marcelo Adrián
Linares, Jorge Alberto
López, María Marcela
Bachmeier, Evelin
Wietz, Fernando Martín
Galván Josa, Víctor Martín
Valentinuzzi, Maria Cecilia
Riveros de la Vega, Jose Alberto
Finkelberg, Ana Beatriz
author Mazzeo, Marcelo Adrián
author_facet Mazzeo, Marcelo Adrián
Linares, Jorge Alberto
López, María Marcela
Bachmeier, Evelin
Wietz, Fernando Martín
Galván Josa, Víctor Martín
Valentinuzzi, Maria Cecilia
Riveros de la Vega, Jose Alberto
Finkelberg, Ana Beatriz
author_role author
author2 Linares, Jorge Alberto
López, María Marcela
Bachmeier, Evelin
Wietz, Fernando Martín
Galván Josa, Víctor Martín
Valentinuzzi, Maria Cecilia
Riveros de la Vega, Jose Alberto
Finkelberg, Ana Beatriz
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 5 Fluorouracil
Saliva
Sem-Eds
topic 5 Fluorouracil
Saliva
Sem-Eds
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This work presents a chemical and morphological analysis of samples of saliva taken from patients who were under treatment with intravenous chemotherapy with 5-fluorouracil and leucovorin calcium. Samples of saliva were extracted from fifteen patients during the three stages of the treatment: The initial stage (previous to the chemotherapy), the intermediate stage (during the chemotherapy), and the final stage (twenty-one days after finishing the treatment). An amount of 50 ll was collected in each visit. Chemical contrast images were taken by means of scanning electron microscopy, and X-ray characteristic spectra were obtained from all the studied samples by using an energy dispersive system from all the studied samples. Images that correspond to the intermediate stage showed important differences with respect to the initial and final stages. In addition, X-ray spectra provided information about the present elements in saliva and their relative abundance allowed us to determine variations in the chemical composition. The backscattered electron images and X-ray spectra from the intermediate stage showed clusters of crystals with fluorine content higher than those obtained in initial and final stages. This fact probably indicates the passage of metabolites of 5-fluorouracil and leucovorin calcium from the plasma to the oral cavity. This finding enhances the hypothesis proposed by other authors about the secondary effects of the drugs on the stomatognathic system such as oral mucositis, dysgeusia, and xerostomia with or without hyposalivation.
Fil: Mazzeo, Marcelo Adrián. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Linares, Jorge Alberto. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: López, María Marcela. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Bachmeier, Evelin. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Wietz, Fernando Martín. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
Fil: Galván Josa, Víctor Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Valentinuzzi, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Riveros de la Vega, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Finkelberg, Ana Beatriz. Universidad Nacional de Cordoba. Facultad de Odontologia. Catedra de Fisiologia; Argentina
description This work presents a chemical and morphological analysis of samples of saliva taken from patients who were under treatment with intravenous chemotherapy with 5-fluorouracil and leucovorin calcium. Samples of saliva were extracted from fifteen patients during the three stages of the treatment: The initial stage (previous to the chemotherapy), the intermediate stage (during the chemotherapy), and the final stage (twenty-one days after finishing the treatment). An amount of 50 ll was collected in each visit. Chemical contrast images were taken by means of scanning electron microscopy, and X-ray characteristic spectra were obtained from all the studied samples by using an energy dispersive system from all the studied samples. Images that correspond to the intermediate stage showed important differences with respect to the initial and final stages. In addition, X-ray spectra provided information about the present elements in saliva and their relative abundance allowed us to determine variations in the chemical composition. The backscattered electron images and X-ray spectra from the intermediate stage showed clusters of crystals with fluorine content higher than those obtained in initial and final stages. This fact probably indicates the passage of metabolites of 5-fluorouracil and leucovorin calcium from the plasma to the oral cavity. This finding enhances the hypothesis proposed by other authors about the secondary effects of the drugs on the stomatognathic system such as oral mucositis, dysgeusia, and xerostomia with or without hyposalivation.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11041
Mazzeo, Marcelo Adrián; Linares, Jorge Alberto; López, María Marcela; Bachmeier, Evelin; Wietz, Fernando Martín; et al.; Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System; Wiley; Journal Of Oral Pathology And Medicine; 42; 10; 11-2013; 788-792
0904-2512
1600-0714
url http://hdl.handle.net/11336/11041
identifier_str_mv Mazzeo, Marcelo Adrián; Linares, Jorge Alberto; López, María Marcela; Bachmeier, Evelin; Wietz, Fernando Martín; et al.; Analysis of Saliva Samples from Oncological Patients Treated with 5-Fluorouracil and Leucovorin Calcium by Scanning Electron Microscopy with Energy Dispersive System; Wiley; Journal Of Oral Pathology And Medicine; 42; 10; 11-2013; 788-792
0904-2512
1600-0714
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jop.12078/full
info:eu-repo/semantics/altIdentifier/doi/10.1111/jop.12078
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.004268

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