Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development

Autores
Mazzei, Mariana Paola; Bianchi, Marta Beatriz; Nestares, Graciela María; Ochogavía, Ana Claudia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Capitate glandular trichomes (CGTs) of sunflower (Helianthus annuus L.) are epidermal structures that facilitate interactions between the plant and various floral visitors. This study aimed to investigate the essential oil composition of an anther´s CGTs during different reproductive stages of the sunflower capitulum. We examined the late development of CGTs using differential interference contrast and confocal microscopy to identify the secretory and post-secretory stages of glands during anthesis and post-anthesis of the disc flowers in a sunflower genotype with a high number of CGTs per anther (a fertility restorer, Rf975). Additionally, we analyzed, for the first time, the chemical composition of essential oils secreted by CGTs during anthesis and post-anthesis using gas chromatography–mass spectrometry (GC–MS) on plants grown under field conditions. The GC–MS analysis from CGTs identified 13 organic compounds and their varying proportions at disc flower´s anthesis and post-anthesis. Proportions of Phytol and Hexadecanal were significantly higher during anthesis than post-anthesis. Moreover, we observed an increase in monoterpenoids and sesquiterpenoids at post-anthesis. Among these, α-Pinene and β-Gurjunene were the most abundant compounds in their respective groups. Our investigation reveals phenophase-dependent changes in the essential oil composition of sunflower anther CGTs. These findings suggest a dynamic “chemical code” generated by these glands, which may enhance communication with beneficial insects during anthesis (e.g., pollinators) and subsequently transition to a defensive function against post-pollination threats (e.g., herbivores).
Fil: Mazzei, Mariana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Bianchi, Marta Beatriz. Universidad Nacional de Rosario; Argentina
Fil: Nestares, Graciela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
CAPITATE GLANDULAR TRICHOMES
PHYTOL
HELIANTHUS ANNUUS L.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279020
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive developmentMazzei, Mariana PaolaBianchi, Marta BeatrizNestares, Graciela MaríaOchogavía, Ana ClaudiaCAPITATE GLANDULAR TRICHOMESPHYTOLHELIANTHUS ANNUUS L.https://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Capitate glandular trichomes (CGTs) of sunflower (Helianthus annuus L.) are epidermal structures that facilitate interactions between the plant and various floral visitors. This study aimed to investigate the essential oil composition of an anther´s CGTs during different reproductive stages of the sunflower capitulum. We examined the late development of CGTs using differential interference contrast and confocal microscopy to identify the secretory and post-secretory stages of glands during anthesis and post-anthesis of the disc flowers in a sunflower genotype with a high number of CGTs per anther (a fertility restorer, Rf975). Additionally, we analyzed, for the first time, the chemical composition of essential oils secreted by CGTs during anthesis and post-anthesis using gas chromatography–mass spectrometry (GC–MS) on plants grown under field conditions. The GC–MS analysis from CGTs identified 13 organic compounds and their varying proportions at disc flower´s anthesis and post-anthesis. Proportions of Phytol and Hexadecanal were significantly higher during anthesis than post-anthesis. Moreover, we observed an increase in monoterpenoids and sesquiterpenoids at post-anthesis. Among these, α-Pinene and β-Gurjunene were the most abundant compounds in their respective groups. Our investigation reveals phenophase-dependent changes in the essential oil composition of sunflower anther CGTs. These findings suggest a dynamic “chemical code” generated by these glands, which may enhance communication with beneficial insects during anthesis (e.g., pollinators) and subsequently transition to a defensive function against post-pollination threats (e.g., herbivores).Fil: Mazzei, Mariana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Bianchi, Marta Beatriz. Universidad Nacional de Rosario; ArgentinaFil: Nestares, Graciela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaWiley Blackwell Publishing, Inc2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279020Mazzei, Mariana Paola; Bianchi, Marta Beatriz; Nestares, Graciela María; Ochogavía, Ana Claudia; Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development; Wiley Blackwell Publishing, Inc; Plant Species Biology; 40; 4; 5-2025; 391-4000913-557XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://esj-journals.onlinelibrary.wiley.com/doi/10.1111/1442-1984.70009info:eu-repo/semantics/altIdentifier/doi/10.1111/1442-1984.70009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T11:59:57Zoai:ri.conicet.gov.ar:11336/279020instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 11:59:58.024CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
title Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
spellingShingle Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
Mazzei, Mariana Paola
CAPITATE GLANDULAR TRICHOMES
PHYTOL
HELIANTHUS ANNUUS L.
title_short Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
title_full Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
title_fullStr Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
title_full_unstemmed Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
title_sort Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development
dc.creator.none.fl_str_mv Mazzei, Mariana Paola
Bianchi, Marta Beatriz
Nestares, Graciela María
Ochogavía, Ana Claudia
author Mazzei, Mariana Paola
author_facet Mazzei, Mariana Paola
Bianchi, Marta Beatriz
Nestares, Graciela María
Ochogavía, Ana Claudia
author_role author
author2 Bianchi, Marta Beatriz
Nestares, Graciela María
Ochogavía, Ana Claudia
author2_role author
author
author
dc.subject.none.fl_str_mv CAPITATE GLANDULAR TRICHOMES
PHYTOL
HELIANTHUS ANNUUS L.
topic CAPITATE GLANDULAR TRICHOMES
PHYTOL
HELIANTHUS ANNUUS L.
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Capitate glandular trichomes (CGTs) of sunflower (Helianthus annuus L.) are epidermal structures that facilitate interactions between the plant and various floral visitors. This study aimed to investigate the essential oil composition of an anther´s CGTs during different reproductive stages of the sunflower capitulum. We examined the late development of CGTs using differential interference contrast and confocal microscopy to identify the secretory and post-secretory stages of glands during anthesis and post-anthesis of the disc flowers in a sunflower genotype with a high number of CGTs per anther (a fertility restorer, Rf975). Additionally, we analyzed, for the first time, the chemical composition of essential oils secreted by CGTs during anthesis and post-anthesis using gas chromatography–mass spectrometry (GC–MS) on plants grown under field conditions. The GC–MS analysis from CGTs identified 13 organic compounds and their varying proportions at disc flower´s anthesis and post-anthesis. Proportions of Phytol and Hexadecanal were significantly higher during anthesis than post-anthesis. Moreover, we observed an increase in monoterpenoids and sesquiterpenoids at post-anthesis. Among these, α-Pinene and β-Gurjunene were the most abundant compounds in their respective groups. Our investigation reveals phenophase-dependent changes in the essential oil composition of sunflower anther CGTs. These findings suggest a dynamic “chemical code” generated by these glands, which may enhance communication with beneficial insects during anthesis (e.g., pollinators) and subsequently transition to a defensive function against post-pollination threats (e.g., herbivores).
Fil: Mazzei, Mariana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Bianchi, Marta Beatriz. Universidad Nacional de Rosario; Argentina
Fil: Nestares, Graciela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description Capitate glandular trichomes (CGTs) of sunflower (Helianthus annuus L.) are epidermal structures that facilitate interactions between the plant and various floral visitors. This study aimed to investigate the essential oil composition of an anther´s CGTs during different reproductive stages of the sunflower capitulum. We examined the late development of CGTs using differential interference contrast and confocal microscopy to identify the secretory and post-secretory stages of glands during anthesis and post-anthesis of the disc flowers in a sunflower genotype with a high number of CGTs per anther (a fertility restorer, Rf975). Additionally, we analyzed, for the first time, the chemical composition of essential oils secreted by CGTs during anthesis and post-anthesis using gas chromatography–mass spectrometry (GC–MS) on plants grown under field conditions. The GC–MS analysis from CGTs identified 13 organic compounds and their varying proportions at disc flower´s anthesis and post-anthesis. Proportions of Phytol and Hexadecanal were significantly higher during anthesis than post-anthesis. Moreover, we observed an increase in monoterpenoids and sesquiterpenoids at post-anthesis. Among these, α-Pinene and β-Gurjunene were the most abundant compounds in their respective groups. Our investigation reveals phenophase-dependent changes in the essential oil composition of sunflower anther CGTs. These findings suggest a dynamic “chemical code” generated by these glands, which may enhance communication with beneficial insects during anthesis (e.g., pollinators) and subsequently transition to a defensive function against post-pollination threats (e.g., herbivores).
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279020
Mazzei, Mariana Paola; Bianchi, Marta Beatriz; Nestares, Graciela María; Ochogavía, Ana Claudia; Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development; Wiley Blackwell Publishing, Inc; Plant Species Biology; 40; 4; 5-2025; 391-400
0913-557X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279020
identifier_str_mv Mazzei, Mariana Paola; Bianchi, Marta Beatriz; Nestares, Graciela María; Ochogavía, Ana Claudia; Dynamic essential oil composition of sunflower anther's capitate glandular trichomes during the reproductive development; Wiley Blackwell Publishing, Inc; Plant Species Biology; 40; 4; 5-2025; 391-400
0913-557X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://esj-journals.onlinelibrary.wiley.com/doi/10.1111/1442-1984.70009
info:eu-repo/semantics/altIdentifier/doi/10.1111/1442-1984.70009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.113929

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