The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke

Autores
Martina, Matteo; Zayas, Aldana; Portis, Ezio; Di Nardo, Giovanna; Polli, Maria Francesca; Comino, Cinzia; Gilardi, Gianfranco; Martin, Eugenia Alejandra; Acquadro, Alberto
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Globe artichoke (Cynara cardunculus var. scolymus; 2n = 2x = 34) is a food crop consumed for its immature flower heads. Traditionally, globe artichoke varietal types are vegetatively propagated. However, seed propagation makes it possible to treat the crop as annual, increasing field uniformity and reducing farmers costs, as well as pathogens diffusion. Despite globe artichoke’s significant agricultural value and the critical role of heterosis inthe development of superior varieties, the production of hybrids remains challenging without a reliable system for large-scale industrial seed production. Male sterility (MS) presents a promising avenue for overcoming these challenges by simplifying the hybridization process and enabling cost-effective seed production. However, withinthe Cynara genus, genic male sterility has been linked to three recessive loci in globe artichoke, with no definitive genetic mechanism elucidated to date. A 250 offsprings F2 population, derived from a cross between a MS globe artichoke and a male fertile (MF) cultivated cardoon (C. cardunculus var. altilis) and fitting a monogenic segregationmodel (3:1), was analyzed through BSA-seq, aiming at the identification of genomic regions/genes affecting male sterility. Four QTL regions were identified on chromosomes 4, 12, and 14. By analyzing the sequence around the highest pick on chromosome 14, a cytochrome P450 (CYP703A2) was identified, carrying a deleterious substitution(R/Q) fixed in the male sterile parent. A single dCAPS marker was developed around this SNP, allowing the discrimination between MS and MF genotypes within the population, suitable for applications in plant breeding programs. A 3D model of the protein was generated by homology modeling, revealing that the mutated amino acid is part of a highly conserved motif crucial for protein folding.
Fil: Martina, Matteo. Università di Torino; Italia
Fil: Zayas, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Portis, Ezio. Università di Torino; Italia
Fil: Di Nardo, Giovanna. Università di Torino; Italia
Fil: Polli, Maria Francesca. Università di Torino; Italia
Fil: Comino, Cinzia. Università di Torino; Italia
Fil: Gilardi, Gianfranco. Università di Torino; Italia
Fil: Martin, Eugenia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Acquadro, Alberto. Università di Torino; Italia
Materia
GLOBE ARTICHOKE
BSA-seq
MALE STERILITY
CYP702A3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262691
Acceder
id CONICETDig_2a5749978b00885430f30f49338edbad
oai_identifier_str oai:ri.conicet.gov.ar:11336/262691
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichokeMartina, MatteoZayas, AldanaPortis, EzioDi Nardo, GiovannaPolli, Maria FrancescaComino, CinziaGilardi, GianfrancoMartin, Eugenia AlejandraAcquadro, AlbertoGLOBE ARTICHOKEBSA-seqMALE STERILITYCYP702A3https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Globe artichoke (Cynara cardunculus var. scolymus; 2n = 2x = 34) is a food crop consumed for its immature flower heads. Traditionally, globe artichoke varietal types are vegetatively propagated. However, seed propagation makes it possible to treat the crop as annual, increasing field uniformity and reducing farmers costs, as well as pathogens diffusion. Despite globe artichoke’s significant agricultural value and the critical role of heterosis inthe development of superior varieties, the production of hybrids remains challenging without a reliable system for large-scale industrial seed production. Male sterility (MS) presents a promising avenue for overcoming these challenges by simplifying the hybridization process and enabling cost-effective seed production. However, withinthe Cynara genus, genic male sterility has been linked to three recessive loci in globe artichoke, with no definitive genetic mechanism elucidated to date. A 250 offsprings F2 population, derived from a cross between a MS globe artichoke and a male fertile (MF) cultivated cardoon (C. cardunculus var. altilis) and fitting a monogenic segregationmodel (3:1), was analyzed through BSA-seq, aiming at the identification of genomic regions/genes affecting male sterility. Four QTL regions were identified on chromosomes 4, 12, and 14. By analyzing the sequence around the highest pick on chromosome 14, a cytochrome P450 (CYP703A2) was identified, carrying a deleterious substitution(R/Q) fixed in the male sterile parent. A single dCAPS marker was developed around this SNP, allowing the discrimination between MS and MF genotypes within the population, suitable for applications in plant breeding programs. A 3D model of the protein was generated by homology modeling, revealing that the mutated amino acid is part of a highly conserved motif crucial for protein folding.Fil: Martina, Matteo. Università di Torino; ItaliaFil: Zayas, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Portis, Ezio. Università di Torino; ItaliaFil: Di Nardo, Giovanna. Università di Torino; ItaliaFil: Polli, Maria Francesca. Università di Torino; ItaliaFil: Comino, Cinzia. Università di Torino; ItaliaFil: Gilardi, Gianfranco. Università di Torino; ItaliaFil: Martin, Eugenia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Acquadro, Alberto. Università di Torino; ItaliaBioMed Central2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262691Martina, Matteo; Zayas, Aldana; Portis, Ezio; Di Nardo, Giovanna; Polli, Maria Francesca; et al.; The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke; BioMed Central; BMC Plant Biology; 24; 1; 5-2024; 1-131471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-024-05119-zinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-024-05119-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:17Zoai:ri.conicet.gov.ar:11336/262691instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:17.376CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
title The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
spellingShingle The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
Martina, Matteo
GLOBE ARTICHOKE
BSA-seq
MALE STERILITY
CYP702A3
title_short The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
title_full The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
title_fullStr The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
title_full_unstemmed The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
title_sort The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke
dc.creator.none.fl_str_mv Martina, Matteo
Zayas, Aldana
Portis, Ezio
Di Nardo, Giovanna
Polli, Maria Francesca
Comino, Cinzia
Gilardi, Gianfranco
Martin, Eugenia Alejandra
Acquadro, Alberto
author Martina, Matteo
author_facet Martina, Matteo
Zayas, Aldana
Portis, Ezio
Di Nardo, Giovanna
Polli, Maria Francesca
Comino, Cinzia
Gilardi, Gianfranco
Martin, Eugenia Alejandra
Acquadro, Alberto
author_role author
author2 Zayas, Aldana
Portis, Ezio
Di Nardo, Giovanna
Polli, Maria Francesca
Comino, Cinzia
Gilardi, Gianfranco
Martin, Eugenia Alejandra
Acquadro, Alberto
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GLOBE ARTICHOKE
BSA-seq
MALE STERILITY
CYP702A3
topic GLOBE ARTICHOKE
BSA-seq
MALE STERILITY
CYP702A3
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Globe artichoke (Cynara cardunculus var. scolymus; 2n = 2x = 34) is a food crop consumed for its immature flower heads. Traditionally, globe artichoke varietal types are vegetatively propagated. However, seed propagation makes it possible to treat the crop as annual, increasing field uniformity and reducing farmers costs, as well as pathogens diffusion. Despite globe artichoke’s significant agricultural value and the critical role of heterosis inthe development of superior varieties, the production of hybrids remains challenging without a reliable system for large-scale industrial seed production. Male sterility (MS) presents a promising avenue for overcoming these challenges by simplifying the hybridization process and enabling cost-effective seed production. However, withinthe Cynara genus, genic male sterility has been linked to three recessive loci in globe artichoke, with no definitive genetic mechanism elucidated to date. A 250 offsprings F2 population, derived from a cross between a MS globe artichoke and a male fertile (MF) cultivated cardoon (C. cardunculus var. altilis) and fitting a monogenic segregationmodel (3:1), was analyzed through BSA-seq, aiming at the identification of genomic regions/genes affecting male sterility. Four QTL regions were identified on chromosomes 4, 12, and 14. By analyzing the sequence around the highest pick on chromosome 14, a cytochrome P450 (CYP703A2) was identified, carrying a deleterious substitution(R/Q) fixed in the male sterile parent. A single dCAPS marker was developed around this SNP, allowing the discrimination between MS and MF genotypes within the population, suitable for applications in plant breeding programs. A 3D model of the protein was generated by homology modeling, revealing that the mutated amino acid is part of a highly conserved motif crucial for protein folding.
Fil: Martina, Matteo. Università di Torino; Italia
Fil: Zayas, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Portis, Ezio. Università di Torino; Italia
Fil: Di Nardo, Giovanna. Università di Torino; Italia
Fil: Polli, Maria Francesca. Università di Torino; Italia
Fil: Comino, Cinzia. Università di Torino; Italia
Fil: Gilardi, Gianfranco. Università di Torino; Italia
Fil: Martin, Eugenia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Acquadro, Alberto. Università di Torino; Italia
description Globe artichoke (Cynara cardunculus var. scolymus; 2n = 2x = 34) is a food crop consumed for its immature flower heads. Traditionally, globe artichoke varietal types are vegetatively propagated. However, seed propagation makes it possible to treat the crop as annual, increasing field uniformity and reducing farmers costs, as well as pathogens diffusion. Despite globe artichoke’s significant agricultural value and the critical role of heterosis inthe development of superior varieties, the production of hybrids remains challenging without a reliable system for large-scale industrial seed production. Male sterility (MS) presents a promising avenue for overcoming these challenges by simplifying the hybridization process and enabling cost-effective seed production. However, withinthe Cynara genus, genic male sterility has been linked to three recessive loci in globe artichoke, with no definitive genetic mechanism elucidated to date. A 250 offsprings F2 population, derived from a cross between a MS globe artichoke and a male fertile (MF) cultivated cardoon (C. cardunculus var. altilis) and fitting a monogenic segregationmodel (3:1), was analyzed through BSA-seq, aiming at the identification of genomic regions/genes affecting male sterility. Four QTL regions were identified on chromosomes 4, 12, and 14. By analyzing the sequence around the highest pick on chromosome 14, a cytochrome P450 (CYP703A2) was identified, carrying a deleterious substitution(R/Q) fixed in the male sterile parent. A single dCAPS marker was developed around this SNP, allowing the discrimination between MS and MF genotypes within the population, suitable for applications in plant breeding programs. A 3D model of the protein was generated by homology modeling, revealing that the mutated amino acid is part of a highly conserved motif crucial for protein folding.
publishDate 2024
dc.date.none.fl_str_mv 2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262691
Martina, Matteo; Zayas, Aldana; Portis, Ezio; Di Nardo, Giovanna; Polli, Maria Francesca; et al.; The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke; BioMed Central; BMC Plant Biology; 24; 1; 5-2024; 1-13
1471-2229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262691
identifier_str_mv Martina, Matteo; Zayas, Aldana; Portis, Ezio; Di Nardo, Giovanna; Polli, Maria Francesca; et al.; The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke; BioMed Central; BMC Plant Biology; 24; 1; 5-2024; 1-13
1471-2229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-024-05119-z
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-024-05119-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614455351050240
score 13.070432

Publicaciones similares