EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness

Autores
Gimeno Valiente, F.; Riffo Campos, Á. L.; Ayala, G.; Tarazona, N.; Gambardella, V.; Rodríguez, Fernanda Mariel; Huerta, M.; Martínez-Ciarpaglini, C.; Montón Bueno, J.; Roselló, S.; Roda, D.; Cervantes, A.; Franco, L.; López Rodas, G.; Castillo, J.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The finding of novel molecular markers for prediction or prognosis of invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation of EPDR1 in a prospective cohort of 101 CRC patients, in a cDNA array of 43 patients and in in silico analyses. EPDR1 encodes a protein related to ependymins, a family of glycoproteins involved in intercellular contacts. A thorough statistical model allowed us to conclude that the gene is significantly up-regulated in tumour tissues when compared with normal mucosa. These results agree with those obtained by the analysis of three publicly available databases. EPDR1 up-regulation correlates with the TNM staging parameters, especially T and M. Studies with CRC cell lines revealed that the methylation of a CpG island controls EPDR1 expression. siRNA knocking-down and overexpression of the gene following transient plasmid transfection, showed that EPDR1 favours cell proliferation, migration, invasiveness and adhesion to type I collagen fibres, suggesting a role in epithelial to mesenchymal transition. Both statistical and functional analysis correlated EPDR1 overexpression with invasiveness and dissemination of tumour cells, supporting the inclusion of EPDR1 in panels of genes used to improve molecular subtyping of CRC. Eventually, EPDR1 may be an actionable target.
Fil: Gimeno Valiente, F.. No especifíca;
Fil: Riffo Campos, Á. L.. Universidad de La Frontera; Chile
Fil: Ayala, G.. Universidad de Valencia; España
Fil: Tarazona, N.. Universidad de Valencia; España
Fil: Gambardella, V.. Universidad de Valencia; España
Fil: Rodríguez, Fernanda Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Huerta, M.. Universidad de Valencia; España
Fil: Martínez-Ciarpaglini, C.. Universidad de Valencia; España
Fil: Montón Bueno, J.. Universidad de Valencia; España
Fil: Roselló, S.. Universidad de Valencia; España
Fil: Roda, D.. Universidad de Valencia; España
Fil: Cervantes, A.. Universidad de Valencia; España
Fil: Franco, L.. Universidad de Valencia; España
Fil: López Rodas, G.. Universidad de Valencia; España
Fil: Castillo, J.. Universidad de Valencia; España
Materia
EPDR1
colorectal
cancer
human
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/145682
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/145682
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasivenessGimeno Valiente, F.Riffo Campos, Á. L.Ayala, G.Tarazona, N.Gambardella, V.Rodríguez, Fernanda MarielHuerta, M.Martínez-Ciarpaglini, C.Montón Bueno, J.Roselló, S.Roda, D.Cervantes, A.Franco, L.López Rodas, G.Castillo, J.EPDR1colorectalcancerhumanhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The finding of novel molecular markers for prediction or prognosis of invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation of EPDR1 in a prospective cohort of 101 CRC patients, in a cDNA array of 43 patients and in in silico analyses. EPDR1 encodes a protein related to ependymins, a family of glycoproteins involved in intercellular contacts. A thorough statistical model allowed us to conclude that the gene is significantly up-regulated in tumour tissues when compared with normal mucosa. These results agree with those obtained by the analysis of three publicly available databases. EPDR1 up-regulation correlates with the TNM staging parameters, especially T and M. Studies with CRC cell lines revealed that the methylation of a CpG island controls EPDR1 expression. siRNA knocking-down and overexpression of the gene following transient plasmid transfection, showed that EPDR1 favours cell proliferation, migration, invasiveness and adhesion to type I collagen fibres, suggesting a role in epithelial to mesenchymal transition. Both statistical and functional analysis correlated EPDR1 overexpression with invasiveness and dissemination of tumour cells, supporting the inclusion of EPDR1 in panels of genes used to improve molecular subtyping of CRC. Eventually, EPDR1 may be an actionable target.Fil: Gimeno Valiente, F.. No especifíca;Fil: Riffo Campos, Á. L.. Universidad de La Frontera; ChileFil: Ayala, G.. Universidad de Valencia; EspañaFil: Tarazona, N.. Universidad de Valencia; EspañaFil: Gambardella, V.. Universidad de Valencia; EspañaFil: Rodríguez, Fernanda Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Huerta, M.. Universidad de Valencia; EspañaFil: Martínez-Ciarpaglini, C.. Universidad de Valencia; EspañaFil: Montón Bueno, J.. Universidad de Valencia; EspañaFil: Roselló, S.. Universidad de Valencia; EspañaFil: Roda, D.. Universidad de Valencia; EspañaFil: Cervantes, A.. Universidad de Valencia; EspañaFil: Franco, L.. Universidad de Valencia; EspañaFil: López Rodas, G.. Universidad de Valencia; EspañaFil: Castillo, J.. Universidad de Valencia; EspañaNature Publishing Group2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/145682Gimeno Valiente, F.; Riffo Campos, Á. L.; Ayala, G.; Tarazona, N.; Gambardella, V.; et al.; EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness; Nature Publishing Group; Scientific Reports; 10; 1; 2-2020; 1-142045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-60476-7info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-60476-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:21Zoai:ri.conicet.gov.ar:11336/145682instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:21.907CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
title EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
spellingShingle EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
Gimeno Valiente, F.
EPDR1
colorectal
cancer
human
title_short EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
title_full EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
title_fullStr EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
title_full_unstemmed EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
title_sort EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
dc.creator.none.fl_str_mv Gimeno Valiente, F.
Riffo Campos, Á. L.
Ayala, G.
Tarazona, N.
Gambardella, V.
Rodríguez, Fernanda Mariel
Huerta, M.
Martínez-Ciarpaglini, C.
Montón Bueno, J.
Roselló, S.
Roda, D.
Cervantes, A.
Franco, L.
López Rodas, G.
Castillo, J.
author Gimeno Valiente, F.
author_facet Gimeno Valiente, F.
Riffo Campos, Á. L.
Ayala, G.
Tarazona, N.
Gambardella, V.
Rodríguez, Fernanda Mariel
Huerta, M.
Martínez-Ciarpaglini, C.
Montón Bueno, J.
Roselló, S.
Roda, D.
Cervantes, A.
Franco, L.
López Rodas, G.
Castillo, J.
author_role author
author2 Riffo Campos, Á. L.
Ayala, G.
Tarazona, N.
Gambardella, V.
Rodríguez, Fernanda Mariel
Huerta, M.
Martínez-Ciarpaglini, C.
Montón Bueno, J.
Roselló, S.
Roda, D.
Cervantes, A.
Franco, L.
López Rodas, G.
Castillo, J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EPDR1
colorectal
cancer
human
topic EPDR1
colorectal
cancer
human
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The finding of novel molecular markers for prediction or prognosis of invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation of EPDR1 in a prospective cohort of 101 CRC patients, in a cDNA array of 43 patients and in in silico analyses. EPDR1 encodes a protein related to ependymins, a family of glycoproteins involved in intercellular contacts. A thorough statistical model allowed us to conclude that the gene is significantly up-regulated in tumour tissues when compared with normal mucosa. These results agree with those obtained by the analysis of three publicly available databases. EPDR1 up-regulation correlates with the TNM staging parameters, especially T and M. Studies with CRC cell lines revealed that the methylation of a CpG island controls EPDR1 expression. siRNA knocking-down and overexpression of the gene following transient plasmid transfection, showed that EPDR1 favours cell proliferation, migration, invasiveness and adhesion to type I collagen fibres, suggesting a role in epithelial to mesenchymal transition. Both statistical and functional analysis correlated EPDR1 overexpression with invasiveness and dissemination of tumour cells, supporting the inclusion of EPDR1 in panels of genes used to improve molecular subtyping of CRC. Eventually, EPDR1 may be an actionable target.
Fil: Gimeno Valiente, F.. No especifíca;
Fil: Riffo Campos, Á. L.. Universidad de La Frontera; Chile
Fil: Ayala, G.. Universidad de Valencia; España
Fil: Tarazona, N.. Universidad de Valencia; España
Fil: Gambardella, V.. Universidad de Valencia; España
Fil: Rodríguez, Fernanda Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Huerta, M.. Universidad de Valencia; España
Fil: Martínez-Ciarpaglini, C.. Universidad de Valencia; España
Fil: Montón Bueno, J.. Universidad de Valencia; España
Fil: Roselló, S.. Universidad de Valencia; España
Fil: Roda, D.. Universidad de Valencia; España
Fil: Cervantes, A.. Universidad de Valencia; España
Fil: Franco, L.. Universidad de Valencia; España
Fil: López Rodas, G.. Universidad de Valencia; España
Fil: Castillo, J.. Universidad de Valencia; España
description The finding of novel molecular markers for prediction or prognosis of invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation of EPDR1 in a prospective cohort of 101 CRC patients, in a cDNA array of 43 patients and in in silico analyses. EPDR1 encodes a protein related to ependymins, a family of glycoproteins involved in intercellular contacts. A thorough statistical model allowed us to conclude that the gene is significantly up-regulated in tumour tissues when compared with normal mucosa. These results agree with those obtained by the analysis of three publicly available databases. EPDR1 up-regulation correlates with the TNM staging parameters, especially T and M. Studies with CRC cell lines revealed that the methylation of a CpG island controls EPDR1 expression. siRNA knocking-down and overexpression of the gene following transient plasmid transfection, showed that EPDR1 favours cell proliferation, migration, invasiveness and adhesion to type I collagen fibres, suggesting a role in epithelial to mesenchymal transition. Both statistical and functional analysis correlated EPDR1 overexpression with invasiveness and dissemination of tumour cells, supporting the inclusion of EPDR1 in panels of genes used to improve molecular subtyping of CRC. Eventually, EPDR1 may be an actionable target.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/145682
Gimeno Valiente, F.; Riffo Campos, Á. L.; Ayala, G.; Tarazona, N.; Gambardella, V.; et al.; EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness; Nature Publishing Group; Scientific Reports; 10; 1; 2-2020; 1-14
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/145682
identifier_str_mv Gimeno Valiente, F.; Riffo Campos, Á. L.; Ayala, G.; Tarazona, N.; Gambardella, V.; et al.; EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness; Nature Publishing Group; Scientific Reports; 10; 1; 2-2020; 1-14
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-60476-7
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-60476-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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